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Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster,Pinctada fucata

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Progress in Natural science:Materials International 2008年第2期18卷 233-238页

作者： Yaopeng Fu a,Liping Xie a,b,,Rongqing Zhang a,b,a Institute of Marine Biotechnology,Department of Biological sciences and Biotechnology,Tsinghua University,beijing 100084,china b Protein science laboratory of the Ministry of Education,Tsinghua University,beijing 100084,china Institute of Marine Biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein science laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster,Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long,with a calculated molec-ular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge,suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.

关键词： 37 kDa laminin receptor precursor Mantle epithelial cell Pearl oyster

Measuring-basis-encrypted six-state quantum key distribution scheme

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Progress in Natural science:Materials International 2006年第1期16卷 84-89页

作者： CHEN Pan1, DENG Fuguo1, WANG Pingxiao1 and LONG Guilu1,2 (Key laboratory for Quantum information and Measurements and Department of Physics, Tsinghua University, beijing 100084, china 2. Key laboratory for Atomic and Molecular Nanosciences, Tsinghua University, beijing 100084, china) [a]Department of Physics Tsinghua University Key Laboratory for Quantum Information and Measurements Beijing China [b]Tsinghua University Key Laboratory for Atomic and Molecular NanoSciences Beijing China

A six-state quantum key distribution scheme with measuring-basis encryption technique, which is based on the six-state protocol and the MBE protocol, is proposed. In this modified six-state quantum key distribution protocol, Alice and Bob use a control key to synchronize the use of their measuring-basis so that they always use the same measuring-basis. This modified six-state quantum key distribution protocol retains the advantage of higher security and at the same time has a higher efficiency.

关键词： quantum key distribution measuring-basis encrypted six-state security.

Comparison of the RF MEMS switches with dielectric layers on the bridge’s lower surface and on the transmission line

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science china(information sciences) 2011年第2期54卷 396-406页

作者： CHEN KenLe & ZHANG JinWen national Key laboratory of Micro/Nano Fabrication technology, Institute of Microelectronics, Peking University, beijing 100871, china 1. national Key laboratory of Micro/Nano Fabrication technology Institute of Microelectronics Peking University Beijing 100871 China

Previously, we proposed that the dielectric layer of RF MEMS switch can be fabricated either on the transmission line, as traditional switches, or on the lower surface of the bridge. This paper presents a detailed comparison of the RF MEMS switches with different positions of dielectric layer. Through theoretically analyzing the physical model of fringing capacitance, it is revealed that different positions of dielectric layer can result in different switch capacitances. Therefore, the change of dielectric-layer position can reduce the switch capacitance and insertion loss when the bridge is much wider than t-line. The theoretical analysis is demonstrated by Maxwell 3D and HFSS simulation, showing that the insertion loss is reduced by around 13% when the bridge width is three times of transmission line width. Further, the inffuence of switch’s structural parameters, such as dielectric material and dimensions of dielectric layer, on the differences between these two kinds of switches is discussed. To achieve high integration together with low-loss and low-actuation-voltage design, the novel switch with dielectric layer on bridge’s lower surface can be expected to have broad applications in the future.

关键词： RF MEMS switch insertion loss fringing capacitance dielectric layer

Facile synthesis of dendrimer-like star-branched poly(isopropylacrylamide) via combination of click chemistry and atom transfer radical polymerization

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science china Chemistry 2010年第12期53卷 2520-2527页

作者： WAN XueJuan,XU Jian & LIU ShiYong CAS Key laboratory of Soft Matter Chemistry Department of Polymer science and Engineering,Hefei national laboratory for Physical sciences at the Microscale,University of science and technology of china,Hefei 230026,china 1. CAS Key laboratory of Soft Matter Chemistry Department of Polymer science and Engineering Hefei National Laboratory for Physical Sciences at the Microscale University of Science and Technology of China Hefei 230026 China

We report a facile synthesis method of dendrimer-like star-branched poly(N-isopropylacrylamide) (PNIPAM) via the combination of click chemistry and atom transfer radical polymerization (ATRP) by employing the arm-first ***,the α-azido-ω-chloro-heterodifunctionalized building block,N3-PNIPAM-Cl (G0-Cl),was synthesized via ATRP by 3-azidopropyl 2-chloropropionate as the *** advantage of click chemistry,the first generation (G1) of dendrimer-like star-branched PNIPAM,G1-(Cl)3,was facilely prepared via the click coupling reaction between G0-Cl and *** the construction of second generation (G2) dendrimer-like star-branched PNIPAM,G2-(Cl)6,terminal chloride moieties of G1-(Cl)3 were first converted to azide,and then reacted with excess tripropargylamine to give G1-(alkynyl)6 ;G2-(Cl)6 was subsequently prepared via click reaction between G1-(alkynyl) 6 and *** permeation chromatography (GPC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry were employed to confirm the successful construction of dendrimer-like star-branched *** unique thermal phase transition behavior of this dendrimer-like star-branched polymer in aqueous solutions was further investigated by turbidimetry and micro-differential scanning calorimetry (Micro-DSC).

关键词： dendrimer-like branched ATRP click chemistry

Crystallization and preliminary X-ray studies of porcine kidney aminoacylase Ⅰ

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Chinese science Bulletin 1997年第20期42卷 1750-1752页

作者： ZHAOHaiyan, MENGWuyi, LIN Zhengjiong and ZHOU Haimeng1. national laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of sciences, beijing 100101, china 2. Department of Biological science and Biotechnology, Tsinghua University, beijing 100084, china national laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing China Department of Biological science and Biotechnology Tsinghua University Beijing China

AMINOACYLASE Ⅰ（ACY-1）（E. C. 3. 5. 1. 14 ） participated in amino acid metabolism existingin mammalian kidney and microorganisms, and catalyzes reversible hydrolysis of acylaminoacids. ACY-1 consists of 772 amino acids, and the molecular weight is 85 500 daltons. It is adimeric metalloprotein having two Zn2+ in the molecule. The nucleotide and the amino acid se-quence of porcine and human ACY-1 have been determined. The nucleotide sequence and

关键词： aminoacylase I crystallization preliminary crystallography.

The influence of thermal radiation on MHD station-point flow past a stretching sheet with heat generation

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Acta Mechanica Sinica 2011年第4期27卷 502-509页

作者： Jing Zhu.Lian-Cun Zheng.Xin-Xin Zhang Department of Mathematics and Mechanics,University of science and technology beijing,100083 beijing,china Thermal Engineering Department,University of science and technology beijing,100083 beijing,china Zheng Department of Mathematics and Mechanics University of Science and Technology Beijing Beijing China Thermal Engineering Department University of Science and Technology Beijing Beijing China

This letter is concerned with the plane and axisymmetric stagnation-point flows and heat transfer of an electrically-conducting fluid past a stretching sheet in the presence of the thermal radiation and heat generation or absorption. The analytical solutions for the velocity distribution and dimensionless temperature profiles are obtained for the various values of the ratio of free stream velocity and stretching velocity, heat source parameter, Prandtl number, thermal radiation parameter, the suction and injection velocity parameter and magnetic parameter and dimensionality index in the series form with the help of homotopy analysis method （HAM）. Convergence of the series is explicitly dis- cussed. In addition, shear stress and heat flux at the surface are calculated.

关键词： Thermal radiation MHD Laminar boundarylayers - Homotopy analysis method

Degradation of 4-chlorophenol in aqueous solution by γ-radiation and ozone oxidation

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science china Chemistry 2006年第2期49卷 186-192页

作者： HU Jun1, WANG Jianlong1,2 & CHEN Rong1 1. laboratory of Environmental technology, Institute of Nuclear Energy technology, Tsinghua University, beijing 100084, china 2. State Key Joint laboratory of Environmental Simulation and Pollution Control, Tsinghua University, beijing 100084, china Correspondence should be addressed to Wang Jianlong 1. laboratory of Environmental technology Institute of Nuclear Energy Technology Tsinghua University Beijing 100084 China 2. State Key Joint laboratory of Environmental Simulation and Pollution Control Tsinghua University Beijing 100084 China

The degradation of 4-chlorophenol (4-CP) by using gamma rays generated by a 60Co source in the presence of O3 was investigated. The radiolysis of 4-CP and the kinetics of 4-CP min-eralization were analyzed based on the determination of total organic carbon (TOC). The influence of initial 4-CP concentration and the free radicals scavengers (such as NaHCO3 and t-butanol) on the 4-CP degradation was also studied. The results showed that when the radiation rate was 336 Gy·min?1, 4-chlorophenol at concentration of 10 mg·L?1 could be completely degraded at the radiation dose of 2 kGy. The degradation of 4-chlorophenol could be described by a first-order reaction model, the rate constant of 4-CP degradation by combined ozonation and radiation was 0.1016 min?1, which was 2.4 times higher than the sum of radiation (0.0294 min?1) and ozonation (0.0137 min?1). It re-vealed that the combination of radiation and ozonation resulted in synergistic effect, which can re-markably increase the degradation efficiency of 4-CP.

关键词： radiolysis, 4-chlorophenol, priority pollutant, degradation, O3, synergistic effect, kinetics.

Antibodies induced by multi-epitope vaccine showed inhibitory activity against heterologous influenza A virus (H3N2)

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Progress in Natural science:Materials International 2006年第1期16卷 105-108页

作者： DING Jian, WU Fan, WEI Wei and CHEN Yinghua (laboratory of Molecular Immunology, Department of Biology, Tsinghua University Protein science laboratory of the Ministry of Education, beijing 100084, china) [a]laboratory of Molecular Immunology Department of Biology Tsinghua University Protein science laboratory of Ministry of Education Beijing China

In this study, recognition of 4 recombinant viral proteins (GST-NHA1) by the antibodies induced by multi-epitope vaccine was testified. Inhibitory activities of these antibodies were also investigated in vitro against four heterologous influenza A viruses (H3N2). Three epitope-specific antibodies purified by affinity chromatography could reduce the plaque formation. Interestingly, the three neutralizing antibodies in combination showed obvious enhancement of inhibitory activity, suggesting that the development of recombinant multi-epitope vaccine might be an effective way against viral mutation.

关键词： influenza A virus recombinant multi-epitope vaccine neutralizing antibody plaque reduction assay.

A new DNA algorithm to solve graph coloring problem

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Progress in Natural science:Materials International 2007年第6期17卷 733-738页

作者： Jiang Xingpeng1, Li Yin2, Meng Ya3 and Meng Dazhi2(1. national laboratory of Pattern Recognition,Institute of Automation, Chinese Academy of sciences, beijing 100080, china 2. College of Applied sciences, beijing University of technology, beijing 100022, china 3. School of science, beijing Institute of technology, beijing 100081, china) national laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences Beijing 100080 China College of Applied sciences Beijing University of Technology Beijing 100022 China School of science Beijing Institute of Technology Beijing 100081 China

Using a small quantity of DNA molecules and little experimental time to solve complex problems successfully is a goal of DNA computing. Some NP-hard problems have been solved by DNA computing with lower time complexity than conventional computing. However, this advantage often brings higher space complexity and needs a large number of DNA encoding molecules. One example is graph coloring problem. Current DNA algorithms need exponentially increasing DNA encoding strands with the growing of problem size. Here we propose a new DNA algorithm of graph coloring problem based on the proof of four-color theorem. This algorithm has good properties of needing a relatively small number of operations in polynomial time and needing a small number of DNA encoding molecules (we need only 6R DNA encoding molecules if the number of regions in a graph is R).

关键词： DNA computing NP-hard problem graph coloring problem.