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Identification of human fetal liver miRNAs by a novel method

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Identification of human fetal liver miRNAs by a novel method

第四届全国RNA进展研讨会

作者： Hanjiang Fu, Yi Tie, Chengwang Xu, Zhuoyuan Zhang, Jie Zhu, Yingxu Shi, Zhixian Sun, Xiaofei Zheng beijing institute of {2. medicine, beijing 100850, china

MicroRNAs (miRNAs) are short 2.-2. nucleotide RNA molecules that have been shown to regulate gene expressions in a variety of eukaryotic systems. miRNAs are widespread in eukaryotes and several hundred of miRNAs have been identified, but still a lot of miRNAs have not been detected in various eukaryotic organisms due to the limitation of traditional miRNA cloning method. Here we describe the identification of 2. miRNAs from a human fetal liver cDNA library by a novel

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SMAD PATHWAY PLAY A ROLE OF IN THE ABNORMALITIES OF LUNG FIBROBLASTS INDUCED BY γ RAY IRradiation

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SMAD PATHWAY PLAY A ROLE OF IN THE ABNORMALITIES OF LUNG FIB...

第十三届国际体视学大会

作者： Yang Li,Ruiyun Peng,Liangwen Song,Leilei Yang,Shaoxia Wang,Dewen Wang beijing institute of {2. medicine No.2. Taiping Road,beijing 100850,china

Objective:radiation pulmonary fibrosis(RPF) is a sequel of both radiotherapy and accidental *** predominant characteristics are massive deposition of extracellular matrix and excessive fibroblast proliferation. Lung fibroblasts(LFs) play a central role in the pathogenesis of ***β1 is a master switch and therapeutic target of *** pathway is the key signal transducer downstream TGFβ*** the studies on the role of Smad pathway in the abnormalities of irradiated LFs might get a new insight into the pathogenesis of RPF. Methods:Rat lung fibroblasts(RLFs ) were isolated from rat lung by trypsin disaggregation to establish a stable model.γray,SB2.3580 or WP631 were used to treat *** changes of the Smad pathway and cellular biological behaviors of RLFs were determined by immunoblotting,immunohistochemistry,coimmunoprecipitation, EMSA,MTT and image anylasis. Results:γray irradiation could stimulate the proliferation,transformation,typeⅠcollagen and PAI-1 expressions of *** the same time,the formation and nucleus translocation of Smad3-4 complex were enhanced. Moreover,γray could facilitate the activation of transcription factors Smad3-4,SP1 and *** with SB2.3580 or WP631,irradiated RLFs expressed less typeⅠcollagen and P-Smad3,and also the activation of transcription factors Smad3-4,SP1 and AP1 in RLFs were inhibited. Conclusions:γray can facilitate the activation of Smad signal pathway in RLFs,and that the activation of this pathway play a role in the proliferation,transformation and typeⅠcollagen expressions of irradiated RLFs.

关键词： γray lung fibroblast Smad pathway

Study on the bulk wave radiation of finite-length SAW devices using FEM/BEM

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Progress in Natural Science:Materials International 2005年第5期15卷 402-406页

作者： WANG Weibiao1*, ZHANG Xiaodong1, SHUI Yongan1, ZHANG De1 and HAN Tao2.1. State Key Laboratory of Modern Acoustics, institute of Acoustics, Nanjing University, Nanjing 2.0093, china 2. Shanghai Jiaotong University, Shanghai 2.0030, china) State Key Laboratory of Modern Acoustics Institute of Acoustics Nanjing University Nanjing 210093 China Shanghai Jiaotong University Shanghai 200030 China

Based on the distributions of stress and free charge on the interface of the surface acoustic wave (SAW) device calculated with the combination of boundary element method and finite element method(FEM/BEM), the power of each of the three possible bulk waves radiated by the SAW device is calculated, and their contributions to the overall input conductance are separated respectively. Moreover, the formula of angular distributions of their power radiation into the substrate is derived. Consider the effect of mass loading, the resistance density,defined as a scalar, is extended to generalized resistance densities which can be written as three 4×4 tensors and for which the formulas are derived. The bulk wave radiations for a synchronous one-port resonator on 42.Y-rotated LiTaO 3 are simulated. It is found that the radiated energy by slow shear wave contributes a very high proportion to input electrical energy in some frequency range.

关键词： SAW device bulk wave radiation FEM/BEM.

太阳辐射下不同地表覆盖物的热反应及对城市热环境的影响

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太阳能学报 2002年第6期23卷 717-720页

作者：邢永杰沈天行刘芳天津大学建筑学院天津100072 北京市太阳能研究所北京100083

该文利用红外热像仪等设备研究了建筑物表面、绿地、柏油路面、水泥砖地面等城市地表覆盖物对太阳辐射的热反应 ,指出在同一天中 ,柏油路面温度最高 ,水泥砖和建筑物表面次之 ,绿地温度最低。通过分析各种覆盖物在城市中所占比例 ,得出... 详细信息

该文利用红外热像仪等设备研究了建筑物表面、绿地、柏油路面、水泥砖地面等城市地表覆盖物对太阳辐射的热反应 ,指出在同一天中 ,柏油路面温度最高 ,水泥砖和建筑物表面次之 ,绿地温度最低。通过分析各种覆盖物在城市中所占比例 ,得出建筑物对城市热环境影响较大的结论 ,并提出了改善城市热环境的几种方法。

关键词：太阳辐射城市地表覆盖物热反应红外热像仪热环境热岛效应

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Structure-efficacy relationships of immunostimulatory activity of CpG-containing oligodeoxynucleotides on mouse spleen cells

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Acta Pharmacologica Sinica 2007年第10期28卷 1637-1644页

作者： Hai-yan DU~3,Shao-you XIA~4,Hai-feng SONG~(2.5),Na LI~2.Ming-mei SHANG~2.Jia ZOU~2.Qing-qing WANG~2.Lun OU~2.XiaoSUN~2.Ai-guo JI~3,Zhong-ming TANG~2.2.Department of Pharmacology and Toxicology,beijing institute of {2. medicine,beijing 100850,china ~3 institute of Biochemical andBiotech Drugs,School of Pharmacy,Shandong University,Ji-nan 2.0012.china ~4 Department of General Surgery,General Hospital of PLA,beijing100853,china DepartmentofPharmacologyandToxicology BeijingInstituteofRadiationMedicineBeijing100850China instituteofBiochemicalandBiotechDrugs SchoolofPharmacyShandongUniversityJi-nan250012China

Aim:To study the relationship between primary structures of oligodeoxynucleo-tides （ODN） containing unmethylated deoxycytidyl-deoxyguanosine （CpG）dinucleotide motifs and their immunostimulatory activities in mouse spleen ***:A series of CpG ODN with different primary structures were *** capabilities to stimulate mouse spleen cell proliferation were determined by[3H]thymidine incorporation *** （interleukin [IL]-6,IL-12.and IFN-α）secretion spectra induced by CpG ODN were assessed by *** ability ofCpG ODN to activate natural killer cells was evaluated by standard 4 *** cytometry was utilized to examine the expressions of various lympho-cyte surface molecules on diverse *** effective CpG ODN for murine,ODN182.,was set as the template of modification and the positive ***:The immunostimulatory activities of CpG ODN with different sequencesand compositions varied markedly,both in character and in *** was uselessfor improving the immunostimulatory activity of ODN182. by simply increasingthe functional hexameric CpG motif number,modifying the site of CpG motifs,orchanging the distance between multi-CpG ***,an addition of a self-complementary palindrome structure at the 3'-end,but not the 5'-end of CpG ODN,aroused marked improvement in its *** designed ODN had superiorcomprehensive immunostimulatory properties compared to ODN ***:The immunostimulatory activity of a CpG ODN was relevant to its primary *** was useless for promoting immunostimulatory activity to simply change CpGmotif number,space,or *** 3'-end palindrome structure of CpG ODN isassociated with enhanced immunostimulatory activity.

关键词： CpG oligodeoxynucleotides immunostimulation structure-activity relationship

A one-dimensional heat transfer model of the Antarctic Ice Sheet and modeling of snow temperatures at Dome A, the summit of Antarctic Plateau

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Science china Earth Sciences 2010年第5期53卷 763-772页

作者： CHEN BaiLian1,3, ZHANG RenHe2. SUN ShuFen1, BIAN LinGen2. XIAO CunDe4,2.& ZHANG TingJun5 1 institute of Atmospheric Physics, Chinese Academy of Sciences, beijing 10002.,{2. 2.Chinese Academy of Meteorological Sciences, beijing 100081,{2. 3 Graduate University of Chinese Academy of Sciences, beijing 100049,{2. 4 Cold and Arid Regions Environmental and Engineering Research institute, Chinese Academy of Sciences, Lanzhou 730000,{2. 5 The National Snow and Ice Data Center, University of Colorado, Boulder, CO 80309-0449, USA institute of Atmospheric Physics Chinese Academy of Sciences Beijing China Graduate University of Chinese Academy of Sciences Beijing China Chinese Academy of Meteorological Sciences Beijing China Cold and Arid Regions Environmental and Engineering Research institute Chinese Academy of Sciences Lanzhou China The National Snow and Ice Data Center University of Colorado Boulder USA

A vertical one-dimensional numerical model for heat transferring within the near-surface snow layer of the Antarctic Ice Sheet was developed based on simplified parameterizations of associated physical processes for the atmosphere, radiation, and snow/ice systems. Using the meteorological data of an automatic weather station (AWS) at Dome A (80°2.′S, 70°2.′E), we applied the model to simulate the seasonal temperature variation within a depth of 2. m. Comparison of modeled results with observed snow temperatures at 4 measurement depths (0.1, 1, 3, 10 m) shows good agreement and consistent seasonal variations. The model results reveal the vertical temperature structure within the near-surface snow layer and its seasonal variance with more details than those by limited measurements. Analyses on the model outputs of the surface energy fluxes show that: 1) the surface energy balance at Dome A is characterized by the compensation between negative net radiation and the positive sensible fluxes, and 2. the sensible heat is on average transported from the atmosphere to the snow, and has an evident increase in spring. The results are considered well representative for the highest interior Antarctic Plateau.

关键词： Antarctic Ice Sheet near-surface layer energy transfer numerical model

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Enhancement of magnetism of Zn0.95Co0.05O films by p-type Cu+ doping

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Progress in Natural Science:Materials International 2011年第1期21卷 31-35页

作者： Wei-chang HAO~(1,5),Jian-jun LI~2.Huai-zhe XU~3,Jia-ou WANG~4,Tian-min WANG~1 1.Center of Materials Physics and Chemistry,Beihang University,beijing 100083,china 2.Department of Materials Science and Engineering, Anhui University of Science and Technology,Huainan 2.2.01,china 3.Department of Physics,Beihang University,beijing 100083,china 4.beijing Synchrotron radiation Facility,institute of High Energy Physics, Chinese Academy of Sciences,100049,china 5.Key Laboratory of Micro-nano Measurement,Manipulation and Physics, Ministry of Education,Beihang University,beijing 100191,china Center of Materials Physics and Chemistry Beihang University Department of Materials Science and Engineering Anhui University of Science and Technology Department of Physics Beihang University Key Laboratory of Micro-nano Measurement Manipulation a

Zn0.95Co0.05O and Zn0.91Co0.05Cu0.04O thin films were fabricated on Si（111） substrate by the reactive magnetron sputtering method at different O-Ar *** characterizations by X-ray diffractometry（XRD）,X-ray photo-electronic spectrum（XPS）, and electron paramagnetic resonance（EPR） indicate that the doped Cu ions substitute the Zn2.ions in the ZnO *** doped Cu ions are in Cu+and Cu2.mixture valence *** ferromagnetism of the Zn0.91Co0.05Cu0.04O film increases gradually the increasing Cu+ions *** results indicate that the increase of ferromagnetism is not owing to the magnetic contribution of Cu+ions themselves,but owing to the enhancement of magnetic interaction between Co ions,which suggests that p-type doping of Cu+ions plays an important role in mediating the ferromagnetic coupling between Co ions.

关键词： Zn0.95Co0.05O films Zn0.91Co0.05Cu0.04O films magnetic semiconductors magnetic coupling co-doping

Identification of miRNAs that alter HepG2.cell proliferation

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Identification of miRNAs that alter HepG2 cell proliferation

第四届全国RNA进展研讨会

作者： Hanjiang Fu, Junjun Feng, Jie Zhu, Yi Tie, Hong Jiang, Yingxu Shi, Zhixian Sun, Xiaofei Zheng beijing institute of {2. medicine, beijing 100850, china

In fetal liver, hepatocyte proliferate at a rapid rate. We were interested to test whether some miRNAs would play an important role in hepatocyte proliferation. From 2. miRNAs discovered in our recent study, we selected 15 newly validated human miRNAs. DNA oligonucleotides of antisense polarity and covering the entire miRNA sequence were synthesized(anti-miDNA). HepG2.cells were transfected with 1.0μM individual DNA oligonucleotides(anti-miDNA) complementary to 15 miRNAs in 96-well plates using Lipofectin Transfection Agent. 72.hr post-transfection, the cell

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SRDAAR－QNPP：a computer code system for the real－time dose assessment of an accident release for Qinshan Nuclear Power Plant

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Journal of Environmental Sciences 1994年第3期6卷 296-309页

作者： Hu Erbang Wang Han(china institute for {2. Protection, Taiyuan 030006, china)

The paper presents a computer code system 'SRDAAR- QNPP' for the real-time dose as-sessment of an accident release for Qinshan Nuclear Power Plant. It includes three parts:thereal-time data acquisition system, assessment computer. and the assessment operating code system. InSRDAAR-QNPP, the wind field of the surface and the lower levels are determined hourly by using amass consistent three-dimension diasnosis model with the topographic following coordinate system.A Lagrangin Puff model under changing meteorological condition is adopted for atmosphericdispersion, the correction for dry and wet depositions. physical decay and partial plume penetrationof the top inversion and the deviation of plume axis caused by complex terrain have been taken in-to account. The calculation domain areas include three square grid areas with the sideline 10 km, 40krn and 160 km and a grid interval 0.5 km, 2.0 km, 8.0 km respectively. Three exposure pathwaysare taken into account:the external exposure from immersion cloud and passing puff, the internalexposure from inhalation and the external exposure from contaminated ground. This system is ableto provide the results of concentration and dose distributions within 10 minutes after the data havebeen inputed.

关键词： real-time dose assessment computer code system nuclear power plant accident.

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