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Technical development of proteomics

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Chinese Science Bulletin 1999年第16期44卷 1441-1447页

作者： WAN Jinghong and HE Fuchu1.Chongqing Medical University, Chongqing 400046, china 2. beijing {2. of radiation medicine, beijing 100850, china Corresponding author Chongqing Medical University Chongqing China beijing institute of {2. medicine Beijing China

Proteome research is an important complementarity for the genome research, which uses quantitative protein-level measurements to characterize biological process. Proteome techniques include two aspects. One is two-dimensional electrophoresis, which is the unique technology to separate the components of proteome. The other is identification of the

关键词： proteome two-dimensional electrophoresis image analysis peptide-mass fingerprinting database.

Effect of wortmannin and 3-AB on G_2.phase arrest and transient failure to activate MPF induced by ionizing irradiation

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Chinese Science Bulletin 1998年第18期43卷 1550-1555页

作者： MENG Xiangbing and SUN Zhixian beijing institute of {2. medicine, beijing 100850, china beijing institute of {2. medicine Beijing China

Active mitosis promoting factor (MPF) composed of p34 cdc2.with cyclin B1 is required for the cell cycle transition from G 2.to M phase. Recent studies have demonstrated that ionizing radiation (IR) exposures associated with inhibition of p34 cdc2.activity was the mechanism of G 2.arrest. At low dose, IR causes transient failure to dephosphorylate p34 cdc2.instead of suppression of cyclin B or MPF formation of cyclin B with p34 cdc2.complex. But the signaling events that regulate p34 cdc2.in irradiated cells remain unclear. This note demonstrates that PI3 kinase family inhibitor wortmannin (wort) and PARP specific inhibitor 3_AB can reduce the G 2.arrest induced by 2.y γ_ray. Immunoprecipitation studies demonstrate that wort and 3_AB can facilitate dephosphorylation of p34 cdc2.in G 2.phase arrest induced by radiation. These findings suggest that wort sensitive pathway and PARP may be involved in initiating the signal transduction of G 2.phase arrest caused by IR.

关键词： G 2 phase arrest ionizing radiation MPF wortmannin 3_AB.

Properties and applications of embryonic stem cells

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Chinese Science Bulletin 2000年第14期45卷 1258-1265页

作者： GUO Xiaoxia & HE Fuchu1. beijing institute of {2. medicine, beijing 100850, china 2. Department of Life Science, Shandong University, Ji’nan 2.0100, china Correspondence should he addressed to He Fuchu beijing institute of {2. medicine Beijing China Department of Life Science Shandong University Ji’nan China

Mouse embryonic stem (ES) cells are pluripotent cells derived from the early embryo and can be propagated stably in undifferentiated state in vitro. They retain the ability to differentiate into all cell types found in the embryonic and adult body in vivo, and can be induced to differentiate into many cell types under appropriate culture conditions in vitro. Using these properties, people have set up various differentiated systems of many cell types and tissues in vitro. Through analysis of these systems, one can identify novel bioactive factors and reveal mechanisms of cell differentiation and organogenesis. ES cell-derived differentiated cells can also be applied to cell transplantation therapy. In addition, we summarized the features and potential applications of human ES cells.

关键词： embryonic stem cell differentiation in vitro cell transplantation therapy gene targeting.

DNA Damage, Signaling and Repair: Protecting genomic integrity and reducing the risk of human disease

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Chinese Science Bulletin 2011年第30期56卷 3119-3121页

作者： ZHOU PingKun Department of radiation Toxicology and Oncology, beijing {2. of radiation medicine, beijing 100850, china Special Topic Editor Department of radiation Toxicology and Oncology Beijing Institute of Radiation Medicine Beijing China

DNA is the genetic material that contains the 'instructions' to not only guide the continuation of life but also govern the development, metabolism and functioning of living organisms. DNA damage occurs daily due to v... 详细信息

关键词： DNA损伤基因组伤信号人类疾病整性修复风险保护

Protein Product Encoded by a Human Novel Gene E9730 Enhances AP-1 Activity through Interacting with Jab1

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 11-15页

作者： Zhao-Qing WANG, Han-Dong WEI, and Fu-Chu HE(Department of System Biology, beijing institute of {2. medicine, Chinese Human National Center at beijing, beijing 100850, china) Department of System Biology Beijing Institute of Radiation Medicine Chinese Human National Center at Beijing Beijing 100850 China

A novel human gene, named E9730 (a clone number of fetal liver cDNA library), has been identified from more than 14,000 expressed sequence tags (ESTs) based on our large scale sequencing of human fetal liver cDNA libraries. Although sequencing of this novel human gene indicates that it is a leucine zipper protein, the function of E9730 and its homologous genes among species is unknown yet. To find out physiological functional clue of E9730, the yeast two-hybrid system was used to screen the E9730-interac-ting protein(s), and one clone containing a cDNA insert with almost the entire coding sequence (amino acids 39-335) of human Jabl (Jun-activating domain binding protein 1) that interacted specifically with E9730 was identified. A specific association between Jabl and E9730 was shown by co-immunoprecipitation and co-localization experiments. Furthermore, E9730 appeared to enhance Jab 1 -induced AP-1 activity in a concentration-dependent manner and Jabl may be involved in the intracellular signaling trans

关键词： human E9730 gene CSN5/Jabl protein-protein interaction signaling transduction

A Putative NES Mediates Cytoplasmic Localization of Apoptin in Normal Cells

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 817-823页

作者： Qing-Ming WANG*, Guo-Cai FAN, Ji-Zhong CHEN, Hui-Peng CHEN, and Fu-Chu HE* Department of Genomics and Proteomics, beijing institute of {2. medicine, beijing 100850, china Department of Genomics and Proteomics Beijing Institute of Radiation MedicineBeijing 100850 China

Apoptin, a protein expressed by chicken anemia virus, is found predominantly in the cytoplasm in normal cells, whereas it localizes in the nucleus in transformed and malignant cells. However, the mecha- nisms that regulate the different subcellular localization of Apoptin in normal and tumor cells have not been fully clarified. In this work, a putative nuclear export signal (NES) in Apoptin was predicted. It was testified that the putative NES (pNES) of Apoptin was not a functional NES, but actually acted as a cytoplasmic retention signal. Deletion of the pNES led to the nuclear accumulation of Apoptin in normal cells. In addition, when a strong nuclear localization signal was introduced into Apoptin, it exclusively translocated to the nucleus in normal cells. These observations indicated that the cytoplasmic localization of Apoptin in normal cells results from the balance between cytoplasmic retention and nuclear import. On the other hand, the pNES was also proved to be necessary for Apoptin multimerization. Mutants lacking the pNES did not form obviously visible globular aggregates in normal or tumor cells.

关键词： Apoptin nuclear localization signal cytoplasmic retention signal nuclear export signal

Expert consensus on radiotherapy combined with immunotherapy for esophageal cancer(2.2. edition)

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radiation medicine and Protection 2024年第2期5卷 59-67页

作者： Professional Committee of radiation Oncology china Anti-Cancer Association Branch of radiation Oncology Chinese Medical Association Branch of radiation Oncology TreatmentPhysician Chinese Medical Doctor Association Qingsong Pang Jun Wang Zhouguang Hui Wencheng Zhang 不详 Tianjin Medical University Cancer institute&Hospital Tianjin 300060China the Fourth Hospital of Hebei Medical University Shijiazhuang 050011China National Cancer Center National Clinical Research Center for CancerCancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Tianjin Medical University Cancer Hospital China

Radiotherapy represents an essential treatment approach for esophageal *** recent years,immunotherapy combined with chemotherapy has become the first-line standard treatment for patients with advanced esophageal *** phase III studies on immunotherapy combined with radiotherapy for locally advanced esophageal cancer are currently *** evidence-based medical data are urgently needed to support the integration of immunotherapy and chemoradiotherapy as a new treatment strategy for patients with locally advanced esophageal *** consensus,formulated based on the latest study results,in-depth research,and thorough discussions,provides a comprehensive set of *** document extensively covers treatment strategies and evaluation methods for radiotherapy combined with immunotherapy across patients with operable esophageal cancer,inoperable locally advanced esophageal cancer,and advanced esophageal ***,common complications and radiation-related issues associated with radiotherapy combined with immunotherapy are discussed,serving as clinical *** expert group comprised members from the Professional Committee of radiation Oncology,{2. Anti-Cancer Association,the Branch of radiation Oncology,Chinese Medical Association,and the Branch of radiation Oncology Treatment Physician,Chinese Medical Doctor Association.

关键词： Esophageal squamous cell carcinoma Esophageal adenocarcinoma Immunotherapy Chemoradiotherapy Radiotherapy Expert consensus

Detection and quantitation of PS2., a phosphorothioate oligodeoxynucleotides in monkey plasma

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Acta Pharmacologica Sinica 2004年第6期25卷 99-104页

作者： Ming-mei SHANG, Hai-feng SONG2. Xiu-wen LIU, Zhong-ming TANG Department of Pharmacology and Toxicology, the institute of radiation {2., Academy of Military Medical Sciences, beijing 100850, china Department of Pharmacology and Toxicology the Institute of Radiation Medicine Academy of Military Medical Sciences

AIM: To establish the method for quantitation of the phosphorothioate oligodeoxynucleotides (S-ODNs) in plasma. METHODS: Two solid-phase extraction columns combined with a strong anion-exchange column were utilized to remove proteins and lipids in plasma, and the salts were removed by a reverse-phase column followed by dialysis with a 2.00 Da-cutoff membrane. The concentration of the tested S-ODNs, PS2., and its metabolites extracted from the plasma were determined by the method of non-gel sieving capillary electrophoresis (NGCE) with diode array detection in the presence of internal standard (IS). RESULTS: The method was with good base number specificity. Relative standard deviation % of both intra and inter assay were all less than 10 %, and the total mean recovery was about 91 %. The methodology was successfully used to determine the PK behavior of an anti-tumor antisense S-ODNs in monkeys and identify the metabolites with single base difference. CONCLUSION: The combined method of solid-phase extraction and NGCE could be used to study the pharmacokinetics of S-ODNs, and the main parameters of the methodology met the requirement of PK study.

关键词： antisense oligodeoxyribonucleotides thionucleotides rhesus monkey plasma solid-phase extraction capillary electrophoresis non-gel sieving internal standard

Therapeutic angiogenesis induced by human hepatocyte growth factor gene in hindlimb ischemia of dogs

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Chinese Science Bulletin 2003年第7期48卷 676-680页

作者： HA Xiaoqin, REN Jianping, BI Jianjin, ZHANG Jinhui & WU Zuze (Wu Chu-tse) beijing institute of {2. medicine, beijing 100850, china beijing institute of {2. medicine

A preclinical study of treating peripheral ar-tery occlusive disease (PAD) was performed by using ahepatocyte growth factor (HGF) gene-expressing vector,plasmid pUDKH, in a dog model with complete ischemia of one hindlimb. After ligation of femoral artery of onehindlimb, pUDKH was transferred directly into the ischemic limb muscles. The angiogenic activity of the plasmid pUDKH was evaluated. On D 30 after injecting once of pUDKH at different doses into local muscles immediately after opera-tion, the degree of augmentation of collateral vessel forma-tion was significantly greater than that treated by blankvector. In addition, the blood flow rate of femoral artery in dogs treated with pUDKH was recovered on D 90, while the flow rate was only 1/5 to 1/3 in control dogs. The pulse am-plitude of pUDKH-treated dogs was recovered on D 90, but it was hardly detectable in most of the control dogs. The sideeffects of intramuscular transfection of pUDKH were alsoinvestigated, and no significant positive change was found. It is suggested that angiogenesis induced by HGF gene has the potential for clinical use in the treatment of peripheral arte-rial diseases.

关键词：外周动脉闭塞疾病肝细胞生长因子基因治疗动物模型下肢局部缺血血管生成