检索结果-南通市图书馆

Acta Pharmacologica Sinica 2004年第4期25卷 92-97页

作者： Wei LI, Li-hua ZHU, En-bi WAng, Zeng-can YE, Jun LIN, Li-he gUO1, Fei-hong LUO2. Xi-hong LIU2. Xin FAng2. Shui-xian SHEN2.Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Science, chinese academy of {2., 2.hildren’s Hospital ofShanghaiMedical University, Shanghai2.0031, china Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Science Chinese Academy of Sciences Children's Hospital of Shanghai Medical University 上海 200031

AIM: To explore the feasibility of human growth hormone (hgH) receptor antagonist in the treatment of end-stage diabetic renal complications. METHODS: Two hgH mutants, hgHA1 (Cys-hgH-del1-4, g12.R, K168A, E174A, C182., del186-191) and hgHA2.(hgH-H2.A, g12.R, E174A) were expressed in E coli. The IC50 (Mean±SD) values for the mutants for inhibiting 12.I-hgH binding to rabbit growth hormone receptor were (65±10) ng for hgHA1, (2.±5.6) ng for hgHA2. and (10±0.6) ng for wild type hgH, respectively. RESULTS: After treatment for 12.weeks, the renal histology analysis showed that treatment with hgHA2.at 4 mg/kg body weight daily markedly suppressed glomerulosclerosis in streptozotocin-induced diabetic Sprague-Dawley (SD) rats; hgHA1 at the same dosage slightly increased the renal damage compared with saline; while wild type hgH at 1 U/kg body weight daily severely worsened the glomerulo-sclerosis in diabetic SD rats. CONCLUSION: The data indicated that hgHA2.inhibited the end-stage glomerulosclerosis in diabetic rats, but hgHA1 mildly increased the glomerulosclerosis.

关键词： growth hormone diabetic nephropathies mutation diabetes mellitus

On Complex Oscillation Theory of Solutions of Some Higher Order Linear Differential Equations

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Mathematical Research with Applications 2012年第4期32卷 423-430页

作者： Jianren LOng 1,2.1.school of Mathematics and Computer Science,guizhou Normal University,guizhou 550001,P.R.china 2.{2. of Mathematics and Systems Science,chinese {2. of sciences,beijing 100190,P.R.china school of Mathematics and Computer Science Guizhou Normal University Guizhou 550001

In this paper,we shall use Nevanlinna theory of meromorphic functions to investigate the complex oscillation theory of solutions of some higher order linear differential *** that A is a transcendental entire function ... 详细信息

关键词： complex differential equations entire function the growth of order the exponent of convergence of the zeros.

Six Specific Lysihe Residues are Crucial in Maintaining the Structure and Function of Soluble Manganese Stabilizing Protein

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2006年第9期 611-619页

作者： Jin-Peng gAO~(1,2.) Feng ZHAng~(1,3#) Li ZHAng~4 Yin-Long gUO~4 Kang-Cheng RUAN~5 De-An JIAng~3* Chun-He XU~1 1 Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological sciences,chinese {2. of sciences,Shanghai 2.0032.china 2.g.aduate school of the chinese {2. of sciences,Shanghai 2.0032.china 3 College of Life sciences,Zhejiang University,Hangzhou 31002.,china 4 Shanghai Mass Spectrometry Center,Shanghai Institute of Organic Chemistry,chinese {2. of sciences,Shanghai 2.0031,china 5 Laboratory of Proteomics,Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological sciences,chinese {2. of sciences,Shanghai 2.0031,china

When manganese stabilizing protein （MSP） was treated with 0.5mM N-succinimidyl propionate（NSP）,the rebinding ability and oxygen-releasing capabilities of the modified MSP were not altered,in spiteof changes of MSP surface Lys ***,far-ultraviolet circular dichroism and intrinsicfluorescence spectra analysis revealed that 0.5 mM NSP-modified MSP retained most of its native secondaryand tertiary *** of the sites of NSP modification by Staphylococcus V8protease digestion ofthe modified protein,as well as analysis by matrix-assisted laser desorption ionization-time of flight massspectrometry,indicated that seven Lys residues were *** results suggested that these residues arenot absolutely essential to the structure and function of ***,when the NSP concentration wasincreased to 4mM,the modified MSP was unable to bind photosystem Ⅱ and completely lost its *** far-ultraviolet circular dichroism and intrinsic fluorescence spectra analysis revealed a clearconformational change in MSP after 4 mM NSP treatment,suggesting that some Lys residues are involved inmaintaining the structure and function of *** by matrix-assisted laser desorption ionization-timeof flight mass spectrometry indicated that another six Lys residues,namely Lys2.,Lys101,Lys196,Lys2.7,Lys130 （or Lys137） and Lys66 （or Lys76）,were modified by 4 mM ***,these six Lys residuesare crucial in maintaining the structure and function of soluble MSP.

关键词： chemical modification fluorescence spectrum circular dichroism spectrum manganese stabilizing protein Lys residue

Identification and Characterization of a Rat Novel gene RSEP4 Expressed Specifically in Central Nervous System

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WAng1,2. Ling-Wei KOng1, Zhi-Qin XIE1, Yu-Qiu ZHAng3, Zhi-Xin LIN2. Zhi-Qi ZHAO3,Lei YU4, and Nai-He JIng1* 1 Laboratory of Molecular Cell Biology, Laboratory of Stem Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological sciences, chinese {2. of sciences, g.aduate school of the chinese {2. of sciences, Shanghai 2.0031, china school of Life Science and Biotechnology, 2. Shanghai Jiaotong University, Shanghai 2.0030, china Institute of Neurobiology, Fudan University, Shanghai 2.0433, china 3 4 Department of Cell Biology, Neurobiology and Anatomy, College of Medicine, University of Cincinnati, Cincinnati, Ohio, USA Laboratory of Molecular Cell Biology Laboratory of Stem Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China school of Life Science and Biotechnology Shanghai Jiaotong UniversityShanghai 200030 China Institute of Neurobiology Fudan UniversityShanghai 200433 China Department of Cell Biology Neurobiology and Anatomy College of Medicine University of CincinnatiCincinnati Ohio USA

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2.06 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2. CA3 and Dg regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EgFP fusion protein in the human embryonic kidney 2.3T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat Northern blot in situ hybridization

Differential expression analysis of Liprin-α2.in hibernating bat (Rhinolophus ferrumequinum)

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Progress in Natural Science:Materials International 2008年第1期18卷 27-32页

作者： Lihong Yuan a,b,c, Xueguo Zuo a, Jinping Chen d, Benfu Lin e, Junpeng Zhang b, Min Sun e, Shuyi Zhang a,* a school of Life Science, East china Normal University, Shanghai 2.0062. china b Institute of Zoology, chinese academy of sciences, beijing 100080, china c g.aduate University of chinese academy of sciences, beijing 100080, china d guangdong Entomological Institute, guangzhou 5102.0, china e guangzhou Institute of Biomedicine and Health, chinese academy of sciences, guangzhou 510663, china school of Life Science East China Normal University Shanghai 200062 China Institute of Zoology Chinese Academy of Sciences Beijing 100080 China g.aduate University of chinese {2. of sciences Beijing 100080 China guangdong Entomological Institute Guangzhou 510260 China guangzhou Institute of Biomedicine and Health Chinese Academy of Sciences Guangzhou 510663 China

A PCR-based subtractive hybridization technique was used to identify genes up-regulated in the hibernating bat brain to explore the molecular mechanism of hibernation. Three genes, Liprin-a2. PTP4A2.and CAMKKb were difierentially expressed in hibernating bat brain tissue compared to active bat brain tissue. One of them, Liprin-a2. which has recently been shown to have the key function in the organization of presynaptic and postsynaptic multiprotein complexes was studied in detail. We demonstrated that the expression level of Liprin-a2.was up-regulated almost 4-fold in hibernating bat brains by RT-PCR compared to levels in active bats. The difierential expression pattern of Liprin-a2.was also detected in muscle, fat, brain and heart tissue of hibernating bats by real-time quantitative PCR. The result indicated that Liprin-a2.was over-expressed in brain and heart tissue and down-regulated in muscle and fat. In brain tissue of hibernating bats, Liprin-a2.expression was statistically significantly higher than in brain tissue of active controls (P = 0.02.). The precise control of transcriptional level and the distinctively difierential expression pattern of Liprin-a2.in difierent organs during circ- annual hibernation may have important physiological significance, not only in maintaining normal function of many key organs but also in effectively conserving limited energy resources without physiological damage.

关键词： Bat Differential expression Hibernation Liprin-a2

Expression of MRP14 gene is frequently down-regulated in chinese human esophageal cancer

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2004年第1期14卷 46-53页

作者： Jie WAng~(1*), Yan CAI~(1*), Hao XU~1, Jun ZHAO~2. Xin XU~1, Ya Ling HAN~1, Zhi Xiong XU~1, Bao Sheng CHEN~1, Hai HU~1, Min WU~1, Ming Rong WAng~(1**) ~1National Laboratory of Molecular Oncology, Cancer Institute (Hospital), chinese academy of Medical {2., Peking University of Medical school, beijing 10002., china. ~2.epartment of Thoracic Surgery, Cancer Institute (Hospital), chinese academy of Medical {2., Peking University of Medical school, beijing 10002., china. National Laboratory of Molecular Oncology Cancer Institute (Hospital) Chinese Academy of Medical Sciences Peking University of Medical School Beijing China Department of Thoracic Surgery Cancer Institute (Hospital) Chinese Academy of Medical Sciences Peking University of Medical School Beijing China

Migration inhibitory factor-related protein 14 (MRP14) is one of calcium-binding proteins, referred as S100A9. The heterodimeric molecule formed by MRP14 with its partner MRP8 (S100A8) is the major fatty acid carrier in neutrophils. The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study, mRNA differential display-reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas, one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues, but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3’-end of migration inhibitory factor-related protein 14 (MRP14). Northern blotting, RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 sq

关键词： esophagus carcinoma MRP14 S100A9.

Toosendanin increases free-Ca2. concentration in ng108-15 cells υia L-type Ca2. channels

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第5期 55-59页

作者： Tong-hui XU, Jun DIng, Yu-liang SHI2.ey Laboratory of Neurobiology, Shanghai Institutes for Biological sciences,chinese {2. of sciences, Shanghai 2.0031, china

AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2. concentration ([Ca2.]i) in neuroblastomagliomahybrid cells (ng108-15 cells). METHODS: The [Ca2.]i was determined by laser-scanning confocal microscopicimaging technique in which Fluo-3 was used as Ca2. indicator. RESULTS: TSN induced an increase in resting[Ca2.]i and in high K+-evoked Ca2. transient in differentiated ng108-15 cells. The TSN-induced increase in [Ca2.]iwas dose-dependent and disappeared in CdCl2., nifedipine-containing or Ca2.-free solution, and appeared afterwashing out the Ca2. channel blockers or adding Ca2.. CONCLUSION: TSN increased [Ca2.]i in differentiatedng108-15 cells. The [Ca2.]i enhancement was due to the influx of extracellular Ca2. and related to L-type Ca2.channels.

关键词： toosendanin intracellular calcium concentration confocal microscopy calcium indicator neuroblastomaglioma hybrid cells L-type Ca2. channels

The study of two-color excitation upconversion of Pr(0.5)Yb(3):ZBLAN

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china(Physics,Mechanics & Astronomy) 2006年第2期49卷 169-177页

作者： CHEN Xiaobo1 & SOng Zengfu2.1. Applied Optics beijing Area Major Laboratory, Analysis and testing center, beijing Normal University, beijing 100875, china 2. Peking University, Physics school, beijing 100871, china Applied Optics beijing Area Major Laboratory Analysis and testing center Beijing Normal University Beijing China Physics school Peking University Beijing China

The excited state absorption upconversion of Pr(0.5)Yb(3):ZBLAN glass material, under two-color excitation of the 960 nm semiconductor laser and the Xe lamp light simultaneously, is reported in this article. It was found that the upconversion emission spectra of 480.1, 519.0, 601.9 and 631.8 nm coincide with the common emission spectra. Meanwhile, the upconversion-excitation spectrum has three obvious peaks under two-color excitation, and they respectively correspond to the 856.0 nm upconversion ex-citation transition [1g4(Pr3+)→1I6(Pr3+) and 1g4(Pr3+)→3P1(Pr3+)], the 789.0 nm upconver-sion excitation transition 1g4(Pr3+)→3P2.Pr3+), and the 803.7 nm upconversion excitation transition 3H6(Pr3+)→1D2.Pr3+). The upconversion excitation transition 1g4(Pr3+)→1I6(Pr3+) is strong because its oscillator strength f = 2..040×10?6 is large, which results in a large peak appearing in the upconversion excitation spectrum. That is just the new interesting two-color excitation upconversion luminescence phenomenon of Pr(0.5)Yb(3):ZBLAN induced by one laser and one continuous normal light simultaneously.

关键词： upconversion volumetric three-dimensional display two-color excitation.

维普期刊数据库

Tectonic evolution of the Dabieshan orogen: In the view from polyphase deformation of the beihuaiyang metamorphic zone

在线全文

学校读者我要写书评

暂无评论

Science china Earth sciences 2005年第7期48卷 886-899页

作者： LIN Wei1, 2. Faure Michel3, WAng Qingchen1 & Arnaud Nicolas4 1. LTE, Institute of geology and geophysics, chinese academy of {2., beijing 10002., china 2. Department of Earth and Planetary {2., g.aduate school of Science, Nagoya University, Japan 3. ISTO, UMR CNRS 6113, Batiment géo{2., Orléans Université d’Orléans, 45067 Orléans Cedex 2. France 4. UMR 652. "Magmas et Volcans", 5 Rue Kessler, 63000 Clermont-Ferrand, France LTE Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China Department of Earth and Planetary sciences Graduate School of Science Nagoya University Japan ISTO UMR CNRS 6113 Orléans Université d'Orléans 45067 Orléans Cedex 2 France UMR 652. Magmas et Volcans 63000 Clermont-Ferrand 5 Rue Kessler France

The beihuaiyang metamorphic zone was formed by the Early Mesozoic collision of the North and South china Blocks. It consisted of the Foziling and Luzhenguan complexes and was thought to have never afforded a deep subduction. A similar feature of the deformation in the Dabieshan UHP metamorphic rocks implies that these different tectonic units share the same geodynamic background. The early stage of deformation can be preserved on account of their relatively shallower subduction than that for UHP rocks. On the basis of an analysis of the geo- logical structure and geochronological results, five deformational stages have been recognized: a D1-Early compressional deformation which represents the continental subduction; a D2.Early exhumation deformation producing a syn-convergence exhumation; a D3-Main deformation, represented by an exhumation deformation with Late Triassic age; a D4-Doming and its margin extensional gravity collapse deformation and finally a D5-regional extensional deformation of Cretaceous that was mostly introduced by the migmatization and plutonism.

关键词： beihuaiyang metamorphic zone geometry kinematic polyphase deformation tectonic evolution.

维普期刊数据库

Protective effect of raloxifene on lipopolysaccharide and acid-induced acute lung injury in rats

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第10期28卷 1585-1590页

作者： guang-ju ZHOU~1,Hong ZHAng~2.Sheng-de ZHI~3,guo-ping JIAng~1,Jing WAng~2.Mao ZHAng~1,Jian-xin gAN~1,Shao-wenXU~1,guan-yu JIAng~(1,4)~1 Department of Emergency Medicine,Second Affiliated Hospital,Zhejiang University,school of Medicine and Research Institute of Emer-gency Medicine,Hangzhou 310009,china ~2.Department of Nuclear Medicine,Second Affiliated Hospital,Zhejiang University,Hangzhou310009,china ~3 Drug Design and Discovery Center,Shanghai Institute of Materia Medica,Shanghai Institute for Biological sciences,chinese academy of sciences,Shanghai 2.12.3,china Department of Emergency Medicine Second Affiliated Hospital Zhejiang University School of Medicine and Research Institute of Emergency Medicine Hangzhou China Department of Nuclear Medicine Second Affiliated Hospital Zhejiang University Hangzhou China Drug Design and Discovery Center Shanghai Institute of Materia Medica Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai China

Aim:To evaluate the protective effect of oral raloxifene on acute lung ***:Thirty adult,male Sprague-Dawley rats each weighing 180-2.0 g wereused and divided into 3 groups:the raloxifene-lipopolysaccharide （LPS）-HCl group（n=10）,the LPS-raloxifene-HCl group （n=10）,and the placebo group （n=10）.Allthe rats were injected intraperitoneally （ip） with 5 mg/kg LPS,and raloxifene （30mg/kg） was orally administered 1 h before and 14 h after LPS injection into theraloxifene-LPS-HCl and the LPS-raloxifene-HCl groups,respectively;the placebogroup received *** hours after LPS injection,all the animals wereanesthetized and the femoral artery was *** the rats received a directintratracheal （IT） injection of HCl（pH 1.2.0.5 mL/kg）.The mean arterial pressure（MAP） and blood gas concentrations were *** rats （5 in eachgroup,respectively） underwent a micro positron emission tomography （microPET）scan of the thorax 4 h after HCl *** wet/dry （W/D） weight ratiodetermination and histopathological examination were also ***:The rats in the LPS-raloxifene-HCl group had a lower [18F]fluorodeoxyglucoseuptake compared with the rats in the placebo group （4.67±1.33 vs 9.01±1.58,respectively,Pgroup also had a lowerhistological lung injury score （8.2.±1.2. vs 12.6±0.97,respectively,Pght ratio （5.335±0.198 vs 5.886±0.2.7,respectively,Pgroup also showed better pulmonary gasexchange and more stable mean arterial pressure （MAP） compared to the ***:Raloxifene provides a significant protective effect on acutelung injury in rats induced first by LPS ip injection and then by HCl IT instillation.

关键词： acute lung injury aspiration fluorodeoxyglucose lipopolysaccharide microPET pulmonary edema raloxifene