Toosendanin increases free-Ca2+ concentration in NG108-15 cells υia L-type Ca2+ channels

作     者：Tong-hui XU, Jun DING, Yu-liang SHI2Key Laboratory of Neurobiology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, Shanghai 200031, China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2004年第5期

页      面：55-59页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by the National Basic Research Program ofChina (No G1999054000) National Natural Science Founda-tion of China (No 39870249 and 30170302) and the Basic Re-search Program (No 02JC14011) 

主　　题：toosendanin intracellular calcium concentration confocal microscopy calcium indicator neuroblastomaglioma hybrid cells L-type Ca2+ channels 

摘      要：AIM: To examine if toosendanin (TSN) affects intracellular free-Ca2+ concentration ([Ca2+]i) in neuroblastomagliomahybrid cells (NG108-15 cells). METHODS: The [Ca2+]i was determined by laser-scanning confocal microscopicimaging technique in which Fluo-3 was used as Ca2+ indicator. RESULTS: TSN induced an increase in resting[Ca2+]i and in high K+-evoked Ca2+ transient in differentiated NG108-15 cells. The TSN-induced increase in [Ca2+]iwas dose-dependent and disappeared in CdCl2-, nifedipine-containing or Ca2+-free solution, and appeared afterwashing out the Ca2+ channel blockers or adding Ca2+. CONCLUSION: TSN increased [Ca2+]i in differentiatedNG108-15 cells. The [Ca2+]i enhancement was due to the influx of extracellular Ca2+ and related to L-type Ca2+channels.