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Expression of lipopolysaccharide binding protein and its receptor CD14 in experimental alcoholic liver disease

World Journal of gastroenterology 2001年第6期7卷 836-840页

作者： guo-Qing Zuo~1 Jian-Ping gong~2.Chang-An Liu~2.Shen-Wei Li~2.Xin-Chuan Wu~2.Kang Yang~2.Yue Li~2.1 Department of Digestive Disease2.Department of general Surgery,Second College of Clinical Medicine &the Second Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400010,china Department of Digestive Disease Second College of Clinical Medicine & the Second Affiliated Hospital Chongqing University of Medical Sciences Chongqing 400010 China. zgqly@***

AIM: To evaluate the relationship between the expression of lipopolysaccharides (LPS) binding protein (LBP) and CD14 mRNA and the severity of liver injury in alcohol-fed rats. METHODS: Twenty Wistar rats were divided into two groups:ethanol-fed group (group E) and control group (group C). group E was fed with ethanol(5-12.g x kg(-1) x d(-1)) and group C received dextrose instead of ethanol. Rats of the two groups were sacrificed at 4 weeks and 8 weeks. Levels of endotoxin and alanine transaminase (ALT) in blood were measured, and liver pathology was observed under light and electronic microscopy. Expressions of LBP and CD14 mRNA in liver tissues were determined by RT-PCR analysis. RESULTS: Plasma endotoxin levels were increased more significantly in group E(12.+/-2.) ng x L(-1) and (187+/-3.) ng x L(-1) at 4 and 8 wk than in control rats(48+/-9) ng x L(-1) and (53./-11) ng x L(-1), respectively (P2.0) nkat x L(-1) and (2.50+/-3.7) nkat x L(-1) in group E. The values were increased more dramatically in ethanol-fed rats than in group C after 4 and 8 weeks. In liver section from ethanol-fed rats, there were marked pathological changes (steatosis, cell infiltration and necrosis). In ethanol-fed rats, ethanol administration led to a significant increase in LBP and CD14 mRNA levels compared with the control group (Pgnificant increase in endotoxin levels in serum and LBP and CD14 mRNA expressions in liver tissues. The increase of LBP and CD14 mRNA expression might wake the liver more sensitive to endotoxin and liver injury.

关键词： Acute-Phase Proteins gene Expression Membrane glycoproteins Animals Antigens, CD14 Carrier Proteins Female Liver Liver Diseases, Alcoholic RNA, Messenger Rats Rats, Wistar Research Support, Non-U.S. gov't Severity of Illness Index

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紫外可见分光光度法测定里松温泉水中硒的含量

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现代化工 2018年第4期38卷 233-235页

作者：陈秋娟谢微苏辉兰黄志强贺州学院材料与环境工程学院广西贺州542899 贺州学院食品与生物工程学院广西贺州542899 贺州学院食品科学与工程技术研究院广西贺州542899

为了测定温泉水中硒的质量浓度,以邻苯二胺为显色剂,采用紫外可见分光光度法测定里松温泉源头水中硒的质量浓度。实验结果表明,体系测定波长为3.3.nm,硒质量浓度在0.05~0.40μg/m L范围内具有良好的线性关系,其线性方程为y=1.598x+0.099... 详细信息

为了测定温泉水中硒的质量浓度,以邻苯二胺为显色剂,采用紫外可见分光光度法测定里松温泉源头水中硒的质量浓度。实验结果表明,体系测定波长为3.3.nm,硒质量浓度在0.05~0.40μg/m L范围内具有良好的线性关系,其线性方程为y=1.598x+0.099 5,相关系数为0.999 5,回收率为98.5%~101.6%,体系在2.h内稳定。该方法操作简便、准确率高,可用于温泉水中硒质量浓度的测定。

关键词：邻苯二胺紫外可见分光光度法里松温泉水硒

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Structural,morphological and magnetic properties of(Ni_(0.5)Co_(0.5))[ga_(x)gd_(x)Fe_(2.2.)]O_(4)nanoparticles prepared via sonochemical approach

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Journal of Rare Earths 2023年第4期41卷 561-571页

作者： Y.Slimani M.A.Almessiere A.Demir Korkmaz A.Baykal M.A.gondal H.güngünes Sagar E.Shirsath A.Manikandan Department of Biophysics Institute for Research and Medical Consultations(IRMC)Imam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Department of Physics College of ScienceImam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Department of Chemistry Istanbul Medeniyet UniversityUskudarIstanbul34700Turkey Department of Nanomedicine Research Institute for Research and Medical Consultations(IRMC)Imam Abdulrahman Bin Faisal UniversityP.O.Box 1982Dammam31441Saudi Arabia Laser Research group Physics DepartmentIRC-Hydrogen&Energy StorageKing Fahd University of Petroleum&MineralsDhahran31261Saudi Arabia K.A.CARE Energy Research and Innovation Center King Fahd University of Petroleum&MineralsDhahran 31261Saudi Arabia Department of Physics Hitit University19030Cevre Yolu BulvarCorumTurkey school of Materials Science and Engineering University of New South WalesKensingtonSydneyNSW2052Australia Department of Chemistry Bharath Institute of Higher Education and Research(BIHER)Bharath UniversityChennaiTamil Nadu600073India Center for Catalysis and Renewable Energy Bharath Institute of Higher Education and Research(BIHER)ChennaiTamil Nadu600073India

The impact of ga^(3.)and gd^(3.)co-substitution on different types of behavior of Ni-Co nanospinel ferrites(NSFs),(Ni_(0.5)Co_(0.5))[ga_(x)gd_(x)Fe_(2.2.)]O_(4)(0.000≤x≤0.02.),was *** were fabricated through ultrasound *** structure,composition,and spherical morphology of all products were verified by numerous characterization techniques such as X-ray diffraction(XRD),scanning and transmission electron microscopes(SEM and TEM),and selected area electron diffraction(SAED).Mossbauer spectra are composed of two ferromagnetic sextets and one paramagnetic *** spectra were fitted to extract hyperfine parameters of the doped *** sublattices'hyperfine magnetic field decreases with *** isomer shift values show that the Mossbauer spectra are composed of magnetic Fe^(3.)*** magnetization measurements at variable magnetic field(M-H)were investigated via a vibrating sample magnetometer(VSM)at temperatures(T)from 3.0 to 10 *** NSFs disclose ferrimagnetic behavior at both 3.0 and 10 K at which they are soft and hard,*** determined that the remanence magnetization(M_(r)),saturation magnetization(M_(s)),and magneton number(n_(B))decrease with increasing ga^(3.)and gd^(3.)ions *** reduction in these values is predominantly recognized to be the impact of cation redistribution and surface spins on tetrahedral(T_(d))and octahedral(O_(h))*** the ga^(3.)and gd^(3.)contents increase,the coercivity(H_(c))is also found to decrease.

关键词： Spinel ferrites Rare earth substitution Sonochemical synthesis Mossbauer analysis Magnetic properties

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Preparation of Pt/C Catalyst with a New and Simple Organic Sol Method

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Chinese Chemical Letters 2004年第7期15卷 875-878页

作者： YaWenTAng gangLI ChangPengLIU WeiX1ng TianHongLU DepartmentofChemistry NanjingNormalUniversityNanjing210097 ChangchunInstituteofAppliedChemistry ChineseAcademyofSciences.Changchun130022

It is reported for the first time that the Pt/C catalyst can be prepared with a new and simple organic sol method using SnCl2.as the reductant. It was found that the average size of the Pt particles in the Pt/C catalysts could be controlled with controlling the preparation conditions. The effect of the average sizes of the Pt particles in the Pt/C catalysts obtained with this method on the electrocatalytical activity of the oxidation of methanol was investigated.

关键词： Pt colloid Pt/C catalyst particle size.

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Reconstructing shock front of unstable detonations based on multi-layer perceptron

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Acta Mechanica Sinica 2021年第11期37卷 1610-1623,I0001页

作者： Lin Zhou Honghui Teng Hoi Dick ng Pengfei Yang Zonglin Jiang school of Aerospace Engineering Beijing Institute of TechnologyBeijing100081China State Key Laboratory of Laser Propulsion and Application Beijing Power Machinery InstituteBeijing100074China Department of Mechanical Industrial and Aerospace EngineeringConcordia UniversityMontrealQCH3G 1M8Canada State Key Laboratory of High Temperature gas Dynamics Institute of MechanicsChinese Academy of SciencesBeijing100190China school of Engineering Sciences University of Chinese Academy of SciencesBeijing100049China

The dynamics of frontal and transverse shocks in gaseous detonation waves is a complex phenomenon bringing many difficulties to both numerical and experimental *** laser-optical visualization of detonation structure may provide certain information of its reactive front,but the corresponding lead shock needs to be reconstructed building the complete flow *** the multi-layer perceptron(MLP)approach,we propose a shock front reconstruction method which can predict evolution of the lead shock wavefront from the state of the reactive *** method is verified through the numerical results of one-and two-dimensional unstable detonations based on the reactive Euler equations with a one-step irreversible chemical reaction *** show that the accuracy of the proposed method depends on the activation energy of the reactive mixture,which influences prominently the cellular detonation instability and hence,the distortion of the lead shock *** select the input variables for training and evaluate their influence on the effectiveness of the proposed method,five groups,one with six variables,and the other with four variables,are tested and analyzed in the MLP *** trained MLP is tested in the cases with different activation energies,demonstrates the inspiring generalization *** paper offers a universal framework for predicting detonation frontal evolution and provides a novel way to interpret numerical and experimental results of detonation waves.

关键词： Cellular detonation Lead shock evolution Multi-layer perceptron Numerical simulations

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A CASE STUDY ON CONCENTRATION AND DECENTRALIZATION:BEHAVIOR AND DYN AMIC MECHANISM OF SPATIAL EVOLUTION IN METROPOLITAN AREA,NANJIng,china

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Chinese geographical Science 2002年第3期12卷 212-217页

作者： ZHU Xi-gang,ZHAng Jing-xiang,CHEN Hu (Department of Urban and Resources Sciences,Nanjing University,Nanjin g 2.0093.P.R.china,) Department of Urban and Resources Sciences Nanjing University Nanjing P. R. China

In rapid socio-economic developme nt,the process of concentration and dispersal of various elements tends to be more dramatic,tremendously in fluencing the shaping and transform ation of the space in metropolitan *** of spatial concentration and decent ralization has thus become a basic me thod in examining metropolitan spatial *** this research,three elements were selected as the essential indicato rs of the process:demographic density distribu-tion,employment density distribut ion and business office *** of these elements in Nanji ng City was exam-ined *** Nanjing City c ould be regarded as a representative of metropolitan areas in china,its s ituation large-ly suggestes the general characteristics in similar areas of *** based on the investigation of Nanji ng City,four general implications were highligh ***,metropolitan areas inchina are in a violentprocess and shift of spatialconcentra-tion and ***,from now to at least the near future,c oncentration will continue to be the central ***,the landscape of metrop olitan areas basically exhibits a dual structure *** gap in en vironmental and ecological qualities among different districts will continue for a l ong ***,Central Business District(CBD)is playing an important role in helpi ng to convert the traditionally single-centered city structure into a polycentric one.

关键词： concentration decentralization dynamic mechanism nanjing metropolis

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长江三角洲地区典型湖泊表层沉积物中有机氯农药DDT、HCH的残留特征与风险评估

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土壤通报 2009年第5期40卷 1171-1175页

作者：舒卫先李世杰淮河流域水资源保护局安徽蚌埠233001 湖泊与环境国家重点实验室中国科学院南京地理与湖泊研究所江苏南京210008

在长江三角洲地区的天目湖和太湖梅梁湾分别采集7个表层沉积物(采样深度:0～2.m)样品,利用gC-μECD测定了样品中HCHs(α-HCH、β-HCH、γ-HCH和δ-HCH)和DDTs(o,p'-DDE、p,p'-DDE、p,p'-DDD、o,p'-DDT和p,p'-DDT)残留量。结果表明,天目湖表层沉积物中DDTs残留量介于0.88～4.3.0}gg-1,平均值为2.59ngg-1;HCHs残留量介于0.76～3.83.gg-1,平均值为2.2.ngg-1。太湖梅梁湾表层沉积物中DDTs残留量介于5.70～15.3.ngg-1,平均值为10.51ngg-1;HCHs残留量介于3.09～6.63.gg-1,平均值为4.84ngg-1。空间分布特征受河流输入污染物影响,天目湖表层沉积物中OCPs(HCHs和DDTs)残留量大致由南向北递减,太湖梅梁湾表层沉积物中OCPs残留量的高值点均出现在入湖口。在天目湖、太湖梅梁湾表层沉积物中都发现有新DDT输入,特别是太湖梅梁湾周边新DDT输入源分布较广。与沉积物中有机氯农药残留的环境质量标准比较,显示长江三角洲地区湖泊表层沉积物中有机氯农药残留可能对生态环境造成一定的危害,太湖梅梁湾表层沉积物中机氯农药残留的生态风险较高。

关键词：太湖梅梁湾天目湖表层沉积物 HCH DDT

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Testicular volume and clinical correlates of hypothalamic–pituitary–testicular function:A cross-sectional study in obese men

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Asian Journal of Andrology 2020年第4期22卷 354-359页

作者： Mark ng Tang Fui Rudolf Hoermann gary Wittert Mathis grossmann Department of Medicine Austin Health University of MelbourneMelbourneVictoria 3084Australia Department of Endocrinology Austin HealthMelbourneVictoria 3084Australia school of Medicine University of AdelaideAdelaideSouth Australia 5005Australia

The aim of this study was to determine whether testicular volume is correlated with clinical and biochemical markers of hypothalamic–pituitary–testicular(HPT)axis *** was a cross-sectional substudy of a larger randomized controlled trial including obese men,body mass index(BMI)≥3. kg m−2.with a total testosterone level2.nmol l−*** volume was measured by orchidometer,testosterone by liquid chromatography/tandem mass spectrometry,and body composition by dual-energy X-ray *** completed the Aging Males'Symptoms(AMS)score,International Index of Erectile Function-5(IIEF-5),physical function,and handgrip dynamometer ***-nine men participated with a median(interquartile range[IQR])age of 53.1(47.6,59.2.years,BMI of 3..0(3..6,40.5)kg m−2.and a total testosterone of 7.0(6.1,7.9)nmol l−*** testicular volume was 18(IQR:10,2.)*** volume was negatively correlated with BMI(τ=−0.1952.P=0.010)and total fat mass(τ=−0.2.15,P=0.005)independent of age and *** BMI,testosterone,sex hormone-binding globulin(SHBg),and luteinizing hormone(LH)were present in a multivariable model,only BMI(-0.3. ml change in testicular volume per 1 kg m-2.MI;95%CI:−0.74,−0.02.P=0.04)and LH(-0.92.ml change in testicular volume per 1 IU l-1 LH;95%CI:−1.75,−0.095;P=0.03.remained independent significant predictors of testicular *** volume was positively correlated with IIEF-5(τ=0.2.92.P=0.02.),but not related to handgrip strength,physical function tests,or *** obese men,testicular volume is inversely and independently associated with measures of adiposity,but not with most clinical or biochemical markers of HPT axis *** a clinical perspective,this suggests that obesity might compromise the reliability of reduced testicular volume as a sign of androgen deficiency in men.

关键词： hypothalamic-pituitary-gonadal axis testicular volume testosterone

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Polyvinylpyrrolidone-based bioink:influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting

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Bio-Design and Manufacturing 2023年第6期6卷 676-690页

作者： Wei Long ng Xi Huang Viktor Shkolnikov Ratima Suntornnond Wai Yee Yeong HP-NTU Digital Manufacturing Corporate Lab Nanyang Technological University(NTU)Singapore 637460Singapore Singapore Centre for 3. Printing(SC3.P) School of Mechanical and Aerospace EngineeringNanyang Technological University(NTU)Singapore 639798Singapore HP Inc. Palo AltoCA 94304USA

Among the different bioprinting techniques,the drop-on-demand(DOD)jetting-based bioprinting approach facilitates contactless deposition of pico/nanoliter droplets ofmaterials and cells for optimal cell–matrix and cell–cell *** bioinks play a critical role in the bioprinting process,there is a poor understanding of the influence of bioink properties on printing performance(such as filament elongation,formation of satellite droplets,and droplet splashing)and cell health(cell viability and proliferation)during the DOD jetting-based bioprinting *** inert polyvinylpyrrolidone(PVP3.0,molecular weight=3.0 kDa)polymerwas used in this study to manipulate the physical properties of the bioinks and investigate the influence of bioink properties on printing performance and cell *** experimental results showed that a higher bioink viscoelasticity helps to stabilize droplet filaments before rupturing from the nozzle *** highly stretched droplet filament resulted in the formation of highly aligned“satellite droplets,”which minimized the displacement of the satellite droplets away from the predefined ***,a significant increase in the bioink viscosity facilitated droplet deposition on the wetted substrate surface in the absence of splashing and significantly improved the accuracy of the deposited main *** analysis showed that cell-laden bioinks with higher viscosity exhibited higher measured average cell viability(%),as the presence of polymer within the printed droplets provides an additional cushioning effect(higher energy dissipation)for the encapsulated cells during droplet impact on the substrate surface,improves the measured average cell viability even at higher droplet impact velocity and retains the proliferation capability of the printed *** the influence of bioink properties(e.g.,bioink viscoelasticity and viscosity)on printing performance and cell proliferation is important for the formulation

关键词： Biofabrication 3. bioprinting Drop-on-demand bioprinting Bioink properties Polyvinylpyrrolidone

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Impact of ozone assisted ultrasonication pre-treatment on anaerobic digestibility of sewage sludge

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Journal of Environmental Sciences 2015年第7期27卷 29-38页

作者： Xinbo Tian Antoine Prandota Trzcinski Li Leonard Lin Wun Jern ng Advanced Environmental Biotechnology Centre Nanyang Environment and Water Research Institute Nanyang Technological University school of Civil and Environmental Engineering Nanyang Technological University Public Utilities Board Water Reclamation (Plants) Department Nanyang Environment and Water Research Institute Nanyang Technological University

Impact of ultrasonication（ULS） and ultrasonication-ozonation（ULS-Ozone） pre-treatment on the anaerobic digestibility of sewage sludge was investigated with semi-continuous anaerobic reactors at solid retention time（SRT） of 10 and 2. days. The control, ULS and ULS-Ozone reactors produced 2.6, 3.9 and 3.8 m L biogas/g CODfedand the volatile solid（VS） removals were 3..6%, 3..3. and 42.1%, respectively at SRT of 10 days. At SRT of2. days, the biogas yields reached 3.3. 3.7 and 3.3.m L biogas/g CODfedand the VS removal rates were 3..3., 40.9% and 45.3. in the control, ULS and ULS-Ozone reactors, ***-Ozone pre-treatment increased the residual organic amount in the digested *** soluble residual organics were found to contain macromolecules with molecular weights（MW） larger than 500 k Da and smaller polymeric products with MW around 19.4and 7.7 k Da. These compounds were further characterized to be humic acid-like substances with fluorescent spectroscopy analysis.

关键词： Sewage sludge Ultrasound Ozone Pre-treatment Molecular weight Anaerobic digestion

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