Polyvinylpyrrolidone-based bioink:influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting

作     者：Wei Long Ng Xi Huang Viktor Shkolnikov Ratima Suntornnond Wai Yee Yeong Wei Long Ng;Xi Huang;Viktor Shkolnikov;Ratima Suntornnond;Wai Yee Yeong

作者机构：HP-NTU Digital Manufacturing Corporate LabNanyang Technological University(NTU)Singapore 637460Singapore Singapore Centre for 3D Printing(SC3DP)School of Mechanical and Aerospace EngineeringNanyang Technological University(NTU)Singapore 639798Singapore HP Inc.Palo AltoCA 94304USA 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第6期

页      面：676-690页

核心收录：

学科分类：0710[理学-生物学] 08[工学] 0807[工学-动力工程及工程热物理] 0708[理学-地球物理学] 0816[工学-测绘科学与技术] 080502[工学-材料学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：supported under the RIE2020 Industry Alignment Fund-Industry Collaboration Projects(IAF-ICP) Funding Initiative 

主　　题：Biofabrication 3D bioprinting Drop-on-demand bioprinting Bioink properties Polyvinylpyrrolidone 

摘      要：Among the different bioprinting techniques,the drop-on-demand(DOD)jetting-based bioprinting approach facilitates contactless deposition of pico/nanoliter droplets ofmaterials and cells for optimal cell–matrix and cell–cell *** bioinks play a critical role in the bioprinting process,there is a poor understanding of the influence of bioink properties on printing performance(such as filament elongation,formation of satellite droplets,and droplet splashing)and cell health(cell viability and proliferation)during the DOD jetting-based bioprinting *** inert polyvinylpyrrolidone(PVP360,molecular weight=360 kDa)polymerwas used in this study to manipulate the physical properties of the bioinks and investigate the influence of bioink properties on printing performance and cell *** experimental results showed that a higher bioink viscoelasticity helps to stabilize droplet filaments before rupturing from the nozzle *** highly stretched droplet filament resulted in the formation of highly aligned“satellite droplets,which minimized the displacement of the satellite droplets away from the predefined ***,a significant increase in the bioink viscosity facilitated droplet deposition on the wetted substrate surface in the absence of splashing and significantly improved the accuracy of the deposited main *** analysis showed that cell-laden bioinks with higher viscosity exhibited higher measured average cell viability(%),as the presence of polymer within the printed droplets provides an additional cushioning effect(higher energy dissipation)for the encapsulated cells during droplet impact on the substrate surface,improves the measured average cell viability even at higher droplet impact velocity and retains the proliferation capability of the printed *** the influence of bioink properties(e.g.,bioink viscoelasticity and viscosity)on printing performance and cell proliferation is important for the formulation