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柠檬醛对黄曲霉质膜物理损伤机制的研究

武汉大学学报（理学版） 2002年第2期48卷 217-222页

作者：罗曼蒋立科邹国林安徽农业大学生物工程系安徽合肥23003 武汉大学生命科学学院湖北武汉430072

通过反射干涉对比显微技术和快速图像分析技术,捕获细胞质膜外光晕变化和细胞周边边缘激发变化的信息,并采用闪烁本征模式分解测定柠檬醛作用于黄曲霉质膜前后各模式（m=0,1,2.所对应的平均平方振幅、剪切弹性μ和弹性弯曲模量Kc,与理... 详细信息

通过反射干涉对比显微技术和快速图像分析技术,捕获细胞质膜外光晕变化和细胞周边边缘激发变化的信息,并采用闪烁本征模式分解测定柠檬醛作用于黄曲霉质膜前后各模式（m=0,1,2.所对应的平均平方振幅2.>、剪切弹性μ和弹性弯曲模量Kc,与理论模式光谱相比,研究柠檬醛损伤质膜的物理机制.结果表明,m=1的平动模式主导了光晕波谱,其平均平方振幅呈双指数衰变,该模式几乎与剪切弹性无关,即μ=0;弹性弯曲模量Kc的最小和最大值分别为9.45×10-18J和5.43×10-17J,与柠檬醛的浓度成正相关性.揭示该醛对黄曲霉质膜的损伤,除改变膜的选择通透性和电导率外,同时使质膜平动模式的平均平方振幅呈双指数衰变,弹性弯曲模量Kc增加,从而降低膜伸缩的弹性.

关键词：物理损伤机制柠檬醛黄曲霉质膜反射干涉对比显微镜本征模式分解快速图像分析技术通透性电导率

Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 277-283页

作者： Man LUO1,2. Li-Ke JIANG2. Yao-Xiong HUANG3, Ming XIAO4, Bo LI4, and Guo-Lin ZOU1* 1college of Life Sciences, Wuhan University, Wuhan 430072. China 2.0} of Life Sciences, Anhui {2. University, Hefei 2.0036, China 3 Institute of Biomedical engineering, Ji’nan University, Guangzhou 510632. China 4Ministry of Education Key Laboratory for Biodiversity Science and Ecological engineering, the Institute of Biodiversity Science, Fudan University, Shanghai 2.0433, China college of Life Sciences Wuhan UniversityWuhan 430072 China college of Life Sciences Anhui Agricultural UniversityHefei 230036 China Institute of Biomedical engineering Ji'nan UniversityGuangzhou 510632 China Ministry of Education Key Laboratory for Biodiversity Science and Ecological engineering the Institute of Biodiversity Science Fudan UniversityShanghai 200433 China

Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.

关键词： citral Aspergillus flavus spore multiplex microanalysis techniques quasi-elastic light scattering technique

Bioaccumulation of polychlorinated biphenyls in prawns and a mud crab from mixed-aquaculture ponds

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Acta Oceanologica Sinica 2009年第6期28卷 111-117页

作者： JIANG Jinhua environmental engineering {2. Taizhou College Linhai 317000 China

The bioaccumulation of ten polychlorinated biphenyl （PCB） congeners was investigated within different tissues in three prawns （Penaeusorientalis Kishinouce, Laomedia astacina de Haan and Penaeus vannamei Boone） and one mud crab （Scylla serrata Forsskal） from mixed-aquaculture ponds in Taizhou in April 2.07. The average concentrations （wet mass） of ∑ PCBs in muscle, head, and rind tissues for mean among these prawn species were 7.04, 2..16, and 11.47 ng/g, respectively. The average concentrations of ∑ PCBs in muscle, branchia, and spawn in Scylla serrata were 6.49, 2..13, and 19.76 ng/g, respectively. The concentrations of CB2. were the highest in prawn tissues （2.40 ng/g）, and accounted for 18.60% of ∑ PCBs. The concentration of CB52.was the lowest. The application of principal component analysis （PCA） for the estimation of PCB sources of contamination resulted in first component main contributions of CB101, CB153 and CB2. in the muscle, head, and rind in combined prawn samples, respectively. The enrichment level of PCBs for different tissues varied greatly. The accumulation coefficient of ∑ PCBs in prawn muscle, head, and rind tissues were 396.35, 1 191.79, and 645.91, respectively. For the Scylla serrata, the accumulation coefficients were 365.81, 1 190.50, and 1 113.31 for muscle, branchia, and spawn tissues, respectively. For prawns, the accumulation coefficient of CB153 was the highest in muscle tissues, while CB155 had the highest coefficients in the head and rind tissues. The CB155 coefficient was the highest in spawn tissues of the mud crab.

关键词： bioaccumulation polychlorinated biphenyls prawn Scylla serrata

REMOVAL OF PHOSPHATES FROM WATER BY PILLARED RECTORITE

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Chinese Journal of Reactive Polymers 2005年第Z1期 67-75页

作者： DU Dongyun 1,2.LI Dunshun3 YU Zhongshan2.1 Key Laboratory of Catalysis and Material Science of hubei Province, college of Chemistry and Material Science, South-Central University for Nationalities, Wuhan 430074, China 2.{2. of Chemistry and environmental engineering hubei Normal University, Huangshi 435002. China 3 Daye Non-ferrous Metals Company Limitted, Huangshi 435002. China

The presence of trace phosphates in treated wastewater from municipalities and industries is often responsible for eutrophication problems in lakes, rivers, and other water bodies. In this paper, we report the removal of PO43- from water by using a pillared rectorite that we synthesized recently. The results show that cross-linking can significantly increase the adsorbing capacity of Na-rectorite for phosphates. The pH, the concentrations of F-, NH4+ and COD are main factors, which affect the results for pillared rectorite to adsorb phosphates from water. The OH-, and F- ions decrease the capacity to adsorb phosphates, while the COD and NH4+ ions increase it.

关键词： Pillared Rectorite Removal of Phosphates Lake-Remediation.

Isolation and Expression Analysis of Two Genes Encoding Cinnamate 4-Hydroxylase from Cotton(Gossypium hirsutum)

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Journal of Integrative Agriculture 2014年第10期13卷 2102-2112页

作者： NI Zhi-yong LI Bo Neumann M Peter Lü Meng FAN Ling Institute of Nuclear and Biological Technologies Xinjiang Academy of Agricultural Sciences college of Agronomy Xinjiang Agricultural University Plant Physiology Laboratory Department of EnvironmentalWater and Agricultural EngineeringFaculty of Civil and Environmental EngineeringTechnion-Israel Institute of Technology

Two genes （GhC4H1 and GhC4H2. that encode putative cotton cinnamate 4-hydroxylases that catalyze the second step in the phenylpropanoid pathway were isolated from developing cotton fibers. GhC4H1 and GhC4H2.each contain open reading frames of 1 518 base pairs （bp） in length and both encode proteins consisting of 505 amino acid residues. They are 90.89% identical to each other at the amino acid sequence level and belong to class I of plant C4Hs. GhC4H1 and GhC4H2.genomic DNA are 2.2.7 and 2.161 bp long, respectively, and contain two introns located at conserved positions relative to the coding sequence. GhC4HI and GhC4H2.promoters were isolated and found to contain many cis-elements （boxes P, L and AC-1 element） previously identified in the promoters of other phenylpropanoid pathway genes. Histochemical staining showed GUS expression driven by the GhC4H1 and GhC4H2.promoters in ovules and fibers tissues. GhC4H1 and GhC4H2.were also widely expressed in other cotton tissues. GhC4H2.expression reached its highest level during the elongation stage of fiber development, whereas GhC4H1 expression increased during the secondary wall development period in cotton fibers. Our results contribute to a better understanding of the biochemical role of GhC4H1 and GhC4H2.in cotton fiber development.

关键词： cinnamate 4-hydroxylase Gossypium hirsutum promoter analysis phenylpropanoid pathway

掺杂Eu^(3+)离子的钨酸锂的固相合成及其发光特性

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稀土 2004年第1期25卷 17-19页

作者：杨水金孙聚堂湖北师范学院化学与环境工程系湖北黄石435002 武汉大学化学与分子科学学院湖北武汉430072

采用固相反应法合成了掺杂Eu3+的Li2.O4材料,并通过X射线粉末衍射对其结构进行了表征,掺杂Eu3+的Li2.O4的晶体结构属四方晶系,晶胞参数a=14.2.80 ,c=9.5863 ,属R3(No.148)空间群,测定了其激发光谱和发射光谱,探讨了掺杂Eu3+的Li2.O4的... 详细信息

采用固相反应法合成了掺杂Eu3+的Li2.O4材料,并通过X射线粉末衍射对其结构进行了表征,掺杂Eu3+的Li2.O4的晶体结构属四方晶系,晶胞参数a=14.2.80 ,c=9.5863 ,属R3(No.148)空间群,测定了其激发光谱和发射光谱,探讨了掺杂Eu3+的Li2.O4的发光特性。

关键词： Eu^3+ Li2.O4 合成发光

Phylogenetic diversity of TypeⅠpolyketide synthase genes from sediments of Ardley Island in Antarctica

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Acta Oceanologica Sinica 2011年第6期30卷 104-111页

作者： ZHAO Jing YANG Ning CHEN Xinglin JIANG Qingru ZENG Runying department of Marine Technology and Ocean engineering College of Oceanography and Environmental Science Xiamen University Xiamen 361005 China Key Laboratory of Marine Biogenetic Resources Third Institute of Oceanography State Oceanic Administration Xiamen 361005 China

The diversity of modular polyketide synthase （PKS） genes in sediments of Ardley Island in Antarctica, was studied by restriction fragment length polymorphism （RFLP） analysis. Phylogenetic analysis of 14 amino acid （AA） sequences indicates that the identified ketosynthase （KS） domains were clustered with those from diverse bacterial groups, including Cyanobacteria, γ-Proteobacteria, Actinobacteria, Firmicutes, and some unidentified microorganisms from marine sponge, bryozoan and other environmental samples. The obtained KS domains showed 43%–81% similarity at the AA level to reference sequences in GenBank. Six identified KS domains showed diverse sequences of the motif （VQTACSTS） that was used to identify the hybrid PKS/nonribosomal peptide synthetase （NRPS） enzyme complex, and formed a new branch. These results reveal a high diversity and novelty of PKS genes in antarctic sediments.

关键词： polyketide synthase diversity Antarctica

SIMULATION OF NON-POINT POLLUTANTS EVOLUTION IN COASTAL PLAIN ISLAND-A CASE STUDY OF CHONGMING ISLAND

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Journal of Hydrodynamics 2008年第2期20卷 246-253页

作者： YIN Hai-long JIANG Wen-yan LI Jian-hua college of environmental Science and {2. Tongji University Shanghai 200092 China department of environmental Science East China Normal University Shanghai 200063 China

The coastal plain region usually shows the agricultural dominated industry mode, so more and more attention is paid to non-point pollutants discharge. In this study, the method for assessing the influence of non-point pollutants discharge on river water quality is probed which is concluded as follows：（1） Considering the costal plain island is characterized as nearly even elevation, the conventional gravitational runoff confluence method based on eight-point gravitational flowing directions （DS） is unable to compute the runoff confluence received by the surrounding rivers, so a new method of triangular confluence based on three-points of grid center and the river segments is presented. Further the equations of non-point pollutants loads estimation and non-point pollutants confluence on rivers are presented. （2. The integration mode of non-point pollutants model and river water quality model is presented so as to further assess the non-point pollutants contribution to river water quality. （3） With the Chongming Island, the third largest island in China, as an example, the above-mentioned equations are specified. For this island, the non-point pollutants loads are estimated and linked to the developed water quality model of the river network in the island, and further the non-point pollution evolution in river network is simulated. In this scenario, the non-point pollution contribution to river water quality over the whole island is clearly displayed, and the area where the river water quality is seriously influenced by non-point pollutants discharge is distinctly depicted. This scenario also shows the water quality contribution ratio of non-point pollution to point pollution can be in the range of 55.5% to 44.5% which proves the importance of non-point pollution control in costal plain islands.

关键词： non-point pollution runoffconfluence water quality model Chongming Island

Soil Fertility Indices of Citrus Orchard Land Along Topographic Gradients in the Three Gorges Area of China

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Pedosphere 2011年第6期21卷 782-792页

作者： WU Dian-Ming YU Yuan-Chun XIA Li-Zhong YIN Shi-Xue YANG Lin-Zhang college of Forest Resources and Environment Nanjing Forestry University Nanjing 210037 （China） State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） college of environmental Science and {2. Yangzhou University Yangzhou 22500g （China）

In the Three Gorges Area of China, soil erosion and the resultant non-point source pollution and ecological degradation have endangered agricultural ecosystems and fresh water reservoirs. Although efforts have been undertaken to reduce soil and water losses from slope land used for citrus production, information on the effects of management practices on soil fertility indices is either limited or nonexistent. This study was conducted to compare the effects of 10 years of various management practices, citrus intercropped with white clover (WC), citrus mulched with straw (SM), citrus intercropped with contour hedgerows (CH), citrus orchard land with impermeable membrane (IM), and citrus intercropped with wheat (Triticum aestivum) and peanut (Arachris hypogaea) (WP), as treatments on soil fertility indices with that of the conventional citrus management (CM). Results showed that the soil organic carbon, total and available nitrogen, available potassium, and water-stable aggregate (> 0.2. mm) contents at the 0–5 cm depth were higher for the WC and SM treatments than the CM treatment. There was also spatial variation in soil fertility along slopes of the WC and SM treatments. The soil total and available nitrogen, phosphorus, and potassium, and water-stable aggregate (> 0.2. mm) contents at both the 0–5 and 5–2. cm depths were higher for the CH and IM treatments than the CM treatment. The average soil available nitrogen and available potassium contents were higher for the WP treatment than the CM treatment, but the WP treatment had little effect on the soil organic carbon, total nitrogen, and water-stable aggregate (> 0.2. mm) contents. These suggested that white clover intercropping and straw mulching were the most effective approaches to improve soil fertility in citrus orchard land of the Three Gorges area.

关键词： intercropping slope land soil nutrients soil water-stable aggregates straw mulching