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Cilia and ciliopathies:From Chlamydomonas and beyond

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science china(Life sciences) 2008年第6期51卷 479-486页

作者： PAN JunMin department of biological {2.s and biotechnology, tsinghua university, beijing 100084, china department of biological {2.s and biotechnology Tsinghua University Beijing China

The biological function of motile cilia/flagella has long been recognized. The non-motile primary cilium, once regarded as a vestigial organelle, however, has been found recently to play unexpected roles in mammalian physiology and development. Defects in cilia have profound impact on human health. Diseases related to cilia, collectively called ciliopathies include male infertility, primary cilia dyskinesia, renal cyst formation, blindness, polydactyly, obesity, hypertension, and even mental retardation. Our current understanding of cilia and ciliopathies has been fueled by basic research employing various model organisms including Chlamydomonas, a unicellular green alga. This review article provides a general introduction to the cell biology of cilia and an overview of various cilia-related diseases.

关键词： Chlamydomonas cilia flagellla human disease ciliopathy

Molecular architecture and engineering of spider dragline silk protein

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Progress in Natural science:Materials International 2005年第9期15卷 769-776页

作者： ZHANG Hengmu and LIU Jinyuan(department of biological {2.s and biotechnology, tsinghua university, beijing 100084, china) department of biological {2.s and biotechnology Tsinghua University Beijing 100084 China

Spider dragline silk, which is produced in spider major ampullate gland, is a composite proteinacious fiber with highly repetitive Ala-Gly-rich domain. The unique combination of both high tensile strength and high elasticity makes spider dragline silk superior to almost any other natural or synthetic fibers. Cloning of the genes reveals that the silk is composed of at least two major proteins. Each protein component contains multiple repeats of modular structures that alternate between Ala-rich domains and Gly-rich domains. Molecular engineering not only opens a door to the production of spidroins but also provides a valuable experimental system to test and further establish the relationship between modular structures and mechanical properties. Here, based on our own studies, we review the latest progress of the modular structure and genetic engineering and outline the future prospects.

关键词： spider dragline silk protein modular structure molecular engineering.

Plant protein kinase genes induced by drought, high salt and cold stresses

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Chinese science Bulletin 2000年第13期45卷 1153-1157页

作者： LIU Qiang , ZHANG Yong & Chen Shouyi1. Deparlmenl of biological science and {2., tsinghua university, beijing 100084, china 2. department of biological science and biotechnology, Slate Key Laboratory of Biomembrane and Membrane {2., tsinghua university, beijing 100084, china 3. Institute of Genetics, Chinese Academy of sciences, {12. 100101, china department of biological {2. and biotechnology Tsinghua University Beijing China department of biological {2. and biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

Drought, high salt and cold are three different kinds of environment stresses that severely influence the growth, development and productivity of crops. They all decrease the water state of plant cells, and consequently result in the harm of plant from water deficit. Several genes encoding protein kinases and induced by drought, high salt and low temperature have been isolated from Arabidopsis. These protein kinases include receptor protein kinase (RPK), MAP kinases, ribosomal-protein kinases and transcription-regulation protein kinase. The expression features of these genes and the regulatory roles of these protein kinases in stress response and signal transduction are discussed.

关键词： protein kinase environment stress signal transduction gene expression.

Structure and regulatory function of plant transcription factors

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Chinese science Bulletin 2001年第4期46卷 271-278页

作者： LIU Qiang, ZHANG Guiyou & CHEN Shouyidepartment of biological {2. and biotechnology, tsinghua Univorsity, beijing 100084, china department of biological {2. and biotechnology, State Laboratory of Biomembrane and Membrane biotechnology,{12., beijing 100084, china Institute of Genetics, Chinese Academy of {2.s, beijing 100101, china department of biological {2. and biotechnology Tsinghua University Beijing China department of biological {2. and biotechnology State Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China Institute of Genetics Chinese Academy of Sciences Beijing China

The expression of inducible genes in plants is regulated by specific transcription factors at the transcrip-tional level. A typical transcription factor usually contains a DNA-binding domain, a transcription regulation domain, a dimerization site and a nuclear localization domain. These functional domains define the characteristic, localization and regulatory role of a transcription factor. Transcription factors recognize and bind to specific cis-acting elements or interact with other proteins, and then activate or repress the transcription of target genes by their functional domains. In recent years, elucidation on the structure and function of transcription factors has become an important subject in plant molecular biology.

关键词： transcription factor DNA-binding domain cis-acting element.

Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster,Pinctada fucata

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Progress in Natural science:Materials International 2008年第2期18卷 233-238页

作者： Yaopeng Fu a,Liping Xie a,b,,Rongqing Zhang a,b,a Institute of Marine biotechnology,department of {2. sciences and biotechnology,tsinghua university,beijing 100084,china b Protein science Laboratory of the Ministry of Education,tsinghua university,beijing 100084,china Institute of Marine biotechnology Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Protein science Laboratory of the Ministry of Education Tsinghua University Beijing 100084 China

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster,Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long,with a calculated molec-ular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge,suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.

关键词： 37 kDa laminin receptor precursor Mantle epithelial cell Pearl oyster

A measurement error model for microarray data analysis

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Progress in Natural science:Materials International 2005年第7期15卷 614-620页

作者： ZHOU Yiming and CHENG Jing ( department of biological {2.s and biotechnology, tsinghua university, beijing 100084, china State Key Laboratory of Biomembrane and Membrane biotechnology, tsinghua university, beijing 100084, china National Engineering Research Center for beijing Biochip Technology, 18 Life {2. Parkway, beijing 102.06, china) department of biological {2.s and biotechnology Tsinghua University Beijing 100084 China State Key Laboratory of Biomembrane and Membrane biotechnology Tsinghua University Beijing 100084 China National Engineering Research Center for beijing Biochip Technology 18 Life Science Parkway Beijing 102206 China

Microarray technology has been widely used to analyze the gene expression levels by detecting fluorescence intensity in a high throughput fashion. However, since the measurement error produced from various sources in microarray experiments is heterogeneous and too large to be ignored, we propose here a measurement error model for microarray data processing, by which the standard deviation of the measurement error is demonstrated to be linearly increased with fluorescence intensity. A robust algorithm, which estimates the parameters of the measurement error model from a single microarray without replicated spots, is provided. The model and algorithm for estimating of the parameters from a given data set are tested on both the real data set and the simulated data set, and the result has been proven satisfactory. And, combining the measurement error model with traditional Z-test method, a full statistical model has been developed It can significantly improve the statistical inference for identifying differentially expressed genes.

关键词： microarray measurement error model parameter estimation differentially expressed genes.

A MESSAGE-DRIVING FORMALISM FOR MODELING AND SIMULATION OF MULTI-AGENT SUPPLY CHAIN SYSTEMS

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Journal of Systems science and Systems Engineering 2011年第4期20卷 385-399页

作者： Wenzhe TAN1 Yueting CHAI2.Yi LIU3 1department of Automation, tsinghua university, {2., 100084, china 2.0} of Automation, tsinghua university, {2., 100084, china 3department of Automation, tsinghua university, {2., 100084, china department of Automation Tsinghua University Beijing China

To acquire a competitive advantage in the expanding market, manufacturing enterprises should be able to manage their supply chains as effectively as possible. It is now becoming popular to model supply chains as multi-agent systems and use discrete event simulation to learn more about their behaviors or investigate the implications of alternative configurations. In order to enhance the computational efficiency and keep the simulation credibility, this paper proposes a message-driving formalism for the simulation of multi-agent supply chain systems. Through the message-driving formalism, the problem of shared variables is addressed and the parallel operation of agents is implemented. Simulation experiments with a prototype implementation show that the message-driving formalism is able to provide credible results in significantly less simulation time.

关键词： Supply chain multi-agent system modeling and simulation parallel discrete event simulation message-driving formalism

TEXTURE DETERMINATION OF THIN FILM ZrO_2.BY ELECTRON DIFFRACTION

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Acta Metallurgica Sinica(English Letters) 1993年第12期6卷 472-473页

作者： WANG Yinghua tsinghua university,beijing,{2. department of Materials science and Engineering,tsinghua university,beijing 100084,{2. tsinghua Univ Beijing China

A method for semi-quantitative determination of thin film texture using electron diffrac- tion is *** texture state of a zirconia thin film with a cubic structure has been determined in this way and is presented as an... 详细信息

关键词： texture thin film electron diffraction ZrO_2

Negative regulation of TGF-β signaling in development

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Cell Research 2004年第6期14卷 441-449页

作者： Ye Guang CHEN, An Ming MENG The State Key Laboratory of Biomembrane and Membrane biotechnology, department of {2. sciences and biotechnology, tsinghua university, beijing 100084, china department of biological {2.s and biotechnology The State Key Laboratory of Biomembrane and Membrane Biotechnology Tsinghua University Beijing China

The TGF-β superfamily members have important roles in controlling patterning and tissue formation in both invertebrates and vertebrates. Two types of signal transducers, receptors and Smads, mediate the signaling to regulate expression of their target genes. Despite of the relatively simple signal transduction pathway, many modulators have been found to contribute to a tight regulation of this pathway in a variety of mechanisms. This article reviews the negative regulation of TGF-β signaling with focus on its roles in vertebrate development.

关键词： TGF-β BMP Nodal Activin antagonist development negative regulation.