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Journal of wuhan university of Technology(Materials Science) 2007年第1期22卷 112-116页

作者：刘卫杨祥良 college of Life Science and Technology Huazhong University of Science and Technology

To assess the merits of PEGylated poly （lactic-co-glycolic acid）（PEG-PLGA） nanoparticles as drug carriers for tumor necrosis factor-α receptor blocking peptide （TNFR-BP）, PEG-PLGA copolymer, which could be used to prepare the stealth nanoparticles, was synthesized with methoxypolyethyleneglycol, DL-lactide and glycolide. The structure of PEG-PLGA was confirmed with ^1H-NMR and FT-IR spectroscopy, and the molecular weight （MW） was determined by gel permeation chromatography. Fluorescent FITC-TNFR- BP was chosen as model protein and encapsulated within PEG-PLGA nanoparticles using the double emulsion method. Atomic force microscopy and photon correlation spectroscopy were employed to characterize the stealth nanoparticles fabricated for morphology, size with polydispersity index and zeta potential. Encapsulation efficiency （EE） and the release of FITC-TNFR-BP in nanopartieles in vitro were measured by the fluorescence measurement. The stealth nanoparticles were found to have the mean diameter less than 2.0 nm and zeta potential less than -2. mV. In all nanoparticle formulations, more than 45% of EE were obtained. FITC-TNFR-BP release from the PEG-PLGA nanoparticles exhibited a biphasic pattern, initial burst release and consequently sustained release. The experimental results show that PEG-PLGA nanoparticles possess the potential to develop as drug carriers for controlled release applications of TNFR-BP.

关键词： tumor necrosis factor-α receptor blocking peptide PEG-PLGA stealth nanoparticles ring-opening polymerization controlled and sustained drug release

Synthesis and Biological Activity of N-[N'-(4,5,6-Trisubstitued pyrimidin-2.yl)acetylureido] Benzoic Sulfimide Derivatives

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Chinese Chemical Letters 2000年第7期11卷 581-582页

作者： Tao WANG Si Jia XUE Hai Jian YANG(Department of chemistry ,Central china {2., wuhan 430079 Department of chemistry,Yichun college,Yichun 336000) Department of chemistry Central China University Department of chemistry Yichun College

Fourteen N'- (4,5,6-Trisubstitued pyrimidin-2.y1) -2.chloroacetylureas and fourteen N-[N'- (4,5,6-trisubstitued pyrimidin-2.yl) acetylureido] benzoic sulfimide derivatives were synthesized. Among them twenty-seven are new compounds and their structures have been confirmed by 1HNMR, IR, MS and elemental analysis. The preliminary biological tests showed that some of the target compounds have excellent inhibitory activities against barnyard grass and rape, and the others have a good regulating activity for plant growth.

关键词： Benzoic sulfimide 2.amino-pyrimidine biological activity synthesis

Parallel Implementations of Modeling Dynamical Systems by Using System of Ordinary Differential Equations

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wuhan university Journal of Natural {2. 2003年第S1期8卷 229-233页

作者： Cao Hong-qing, Kang Li-shan, Yu Jing-xianState Key Laboratory of Software Engineering, wuhan university, wuhan {2.hubei,chinacollege of chemistry and molecular sciences, wuhan university, wuhan 430072. hubei, china

First, an asynchronous distributed parallel evolutionary modeling algorithm (PEMA) for building the model of system of ordinary differential equations for dynamical systems is proposed in this paper. Then a series of parallel experiments have been conducted to systematically test the influence of some important parallel control parameters on the performance of the algorithm. A lot of experimental results are obtained and we make some analysis and explanations to them.

关键词： parallel genetic programming evolutionary modeling system of ordinary differential equations

A New Type of Dibenzoyl Tartaric Acid Selective Electrode Based on Polymer Membrane Containing Calixarene Ionophore

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wuhan university Journal of Natural {2. 2003年第1A期8卷 113-116页

作者： Meng L ing\|zhi, Mei Gong\|xiong, He Yong\|bing, Li Jiang\|feng (college of chemistry and {2. sciences, wuhan university, wuhan, 430072. hubei, china) college of chemistry and {2. sciences Wuhan University Wuhan 430072 Hubei China

A new type of di benzoyl tartaric acid selective electr ode has been developed. Three double\| arm calixarene derivatives were emp loyed as the neutral ionophores. The poly(vinyl chloride) membrane electrode containing an amide derivative of ca lixarene as the neutral carrier an d a dibutyl phthalate as the plastici zer exhibited the highest sensitivity for dibenzoyl tartaric acid. The slop e of linear portion was 2..8 mV per c oncertration decade. The electrode has a fast response and a long lifetime .

关键词： ion\|selective electrode calixarene ionophore mole cular recognition dibenzoyl tartaric acid

3,4-Dichlorotoluene Ammoxidation to 3,4-Dichlorobenzonitrile over VPO/SiO_2.Catalyst

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wuhan university Journal of Natural {2. 2002年第3期7卷 353-355页

作者： Huang Chi, Xiao Di, Xu Hai\|xia, Zheng Qiong, Chen Yuan\|yin college of chemistry and {2. sciences, wuhan university, wuhan 430072. hubei. china college of chemistry and {2. sciences Wuhan University Wuhan 430072 Hubei China

Ammoxidation of 3,4\|dichlorotoluene(DCT) to prepare 3,4\|dichlorobenzonitrile (DCBN) over silica supported vanadium phosphorus oxide catalysts has been studied. On the VPO/SiO 2. catalyst, the influence of the reaction temperature, the molar ratio of air/DCT, the molar ratio of NH 3 /DCT in the feed gas and the space velocity ( v s) on the conversion, yield and selectivity was observed. The most appropriate reaction condition is: reaction T =673 K, n (DCT)∶ n (NH 3 )∶ n (air)=1∶7∶30 and v s=2.0 h -1 . At this optimum reaction condition, the conversion of DCT is 97.8%; the molar yield of DCBN is 67.4%. It was found that the addition of element phosphorus can improve the yield of DCBN compared with VO/SiO 2. catalyst.

关键词： ammoxidation 3,4\|dichlorotoluene 3,4\|dichlorobenzonitrile VPO/SiO 2

Density functional theory study of the magnetic properties of rare earth complexes:the magnetic coupling mechanism in Y~Ⅲ and Gd~Ⅲ complexes with nitronyl nitroxide

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Science china chemistry 2009年第11期52卷 1961-1968页

作者： REN Jie1,2.WANG BingWu1 & CHEN ZhiDa1 1 Beijing National Laboratory for molecular sciences,State Key Laboratory of Rare Earth Materials {2. and Applications,college of {2. and molecular Engineering,Peking university,Beijing 100871,china 2.college of Science,Hebei university of Science and Technology,Shijiazhuang 050018,china Beijing National Laboratory for molecular sciences State Key Laboratory of Rare Earth Materials Chemistry and Applications College of Chemistry and Molecular Engineering Peking University Beijing China college of Science Hebei University of Science and Technology Shijiazhuang China

The magnetic coupling interactions of the nitronyl nitroxide radicals bound to diamagnetic(YⅢ) and paramagnetic(GdⅢ) rare earth ions in two model magnetic systems based on novel rare earth organic radical complexes Ln(hfac)3(NITPhOCH3)2.Ln=YⅢ 1,GdⅢ 2.hafc = hexafluoroacetylacetonate;NIT-PhOCH3 = 4'-methoxyo-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) have been investigated by density functional theory(DFT).The magnetic coupling mechanisms were also explored from the viewpoint of molecular orbital and spin density *** calculations show that the empty 4d-orbitals of YⅢ and 5d-orbitals of GdⅢ play an important role in the antiferromagnetic coupling between the two nitronyl nitroxide radical ligands,and that the ferromagnetic coupling between the GdⅢ ion and the radical magnetic centers can be attributed to the nearly complete localization of the isotropic 4f-shell and singly occupied magnetic orbital(π) of the nitronyl nitroxide.

关键词： magnetic coupling density functional theory rare earth ion gadolinium(Ⅲ) complexes yttrium(Ⅲ) complexes

Mechanistic aspects of oxidation of palladium with O2

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中国科学:化学 2012年第10期42卷 1490-1490页

作者： JIN LiQun & LEI AiWen* college of chemistry and {2. sciences,wuhan university,wuhan 430072.china college of chemistry and {2. sciences wuhan university wuhan 430072. china

Palladium-catalyzed aerobic transformation has been one of the most challenging topics within organometallics ***,the corresponding methodology has been developed rapidly,involving alcohol oxidation,alkene oxidation,oxidative coupling and so on,which stimulated considerable interests in mechanistic investigation of the oxidation of Pd with *** review summarizes most of the mechanistic studies on this topic during the past ten ***,the future of the mechanistic investigation for aerobic oxidation of Pd is also discussed.

关键词： palladium O2 mechanism

Trajectory Studies of Chemical Kinetics Using Dual-level Ab Initio molecular Dynamics

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Trajectory Studies of Chemical Kinetics Using Dual-level Ab ...

碱性果胶裂解酶基因pelA在***中的表达和酶学性质研究

第九届全国化学动力学会议

作者： Jicun Li,Baoshan Wang college of chemistry and {2. sciences, wuhan university, wuhan 430072

Classical transition state theory has been widely used in the calculation of rate coefficients of many chemical reactions. Because only some key stationary points are required in the formula, such a method is very easy to use. On the other hand, it involves many drawbacks, for example, the detail of the reaction path cannot be obtained.

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武汉大学学报（理学版） 2001年第4期47卷 477-480页

作者：翟超曹军卫武汉大学生命科学学院湖北武汉430072

根据核苷酸序列分析结果设计引物 ,通过 PCR的方法 ,在嗜碱芽孢杆菌 NTT3 3的总 DNA和 p13 5 0中分别扩增到预计大小的片段 ,证明 p13 5 0中的外源 DNA片段来自于嗜碱芽孢杆菌 NTT3 3 .同时也获得了含有pel A完整 ORF的 DNA片段 .经计... 详细信息

根据核苷酸序列分析结果设计引物 ,通过 PCR的方法 ,在嗜碱芽孢杆菌 NTT3 3的总 DNA和 p13 5 0中分别扩增到预计大小的片段 ,证明 p13 5 0中的外源 DNA片段来自于嗜碱芽孢杆菌 NTT3 3 .同时也获得了含有pel A完整 ORF的 DNA片段 .经计算 ,该基因表达的成熟蛋白质的分子量为 3 4 .76× 10 3.构建表达载体 p BV2.2.0 -pel,在 *** DH5α中进行表达 .SDS- PAGE检测发现 ,表达的蛋白质分子的大小约为 3 5× 10 3,与预测的相符 .初步研究其酶学性质发现 ,该酶在 p H 9.0～ p H10 .0 ,4 5°C的条件下有较高的活性 ,而且必需 Ca2.+ 参与反应 .

关键词：碱性果胶裂解酶基因嗜碱芽孢杆菌 PCR 基因表达 pelA基因 DNA片段酶活性

molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei:Cypriniformes)

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Progress in Natural Science:Materials International 2008年第2期18卷 155-160页

作者： Xianghui Kong a,b,Xuzhen Wang a,Xiaoni Gan a,Junbing Li a,Shunping He a,a Institute of Hydrobiology,Chinese Academy of sciences,wuhan {2.china b college of Life Science,Henan Normal university,Xinxiang 453007,china Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China college of Life Science Henan Normal University Xinxiang 453007 China

Connective tissue growth factor (CTGF) plays an important role in regulation of cell growth,differentiation,apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study,19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification,cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (NJ),maximum parsimony (MP),maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages,respectively,with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini,species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 bp indel (insertion/deletion) was found at the 50 end of CTGF gene of Cyprinidae,and this 6 bp deletion only appeared in the Leuciscini,which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein,the most variations and indels were distributed in the signal region and IGFBP region of this protein,implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity.

关键词： Connective tissue growth factor Sequence variation molecular evolution Cyprinidae