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Hemoglobin-biocatalyzed Synthesis of Conducting molecular Complex of Polyaniline and Lignosulfonate

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Journal of wuhan university of Technology(Materials Science) 2008年第6期23卷 809-815页

作者：胡兴邹国林 Department of Bioengineering Huaihua University State Key Laboratory of Virology College of Life SciencesWuhan University

A new biocatalyst route for the synthesis of a conducting polyaniline （PANI）/ lignosulfonate （LGS） complex was *** different catalysts such as hemoglobin （Hb）,5,10,15,2.-tetrakis （meso-hydroxyphenyl） porphyrin,iron （II） tetrasulfophthalocyanine and ferric chloride were used to polymerize aniline in the presence of a natural polyelectrolytes template *** experimental results show that Hb is an effective catalyst in this case and the synthesis is simple,and the conditions are mild in that the polymerization may be carried out in lower pH （1.0-4.0） buffered solution and optimal pH of *** concentrations of aniline,LGS and H2.2.in feed the favorable conditions for the production of PANI were ***-vis absorption,FTIR,elemental analysis,conductivity,cyclic voltammetry and thermogravimetric analyses confirm the formation of thermally stable and electroactive PANI.

关键词： hemoglobin polyaniline biocatalyst conductivity lignosulfonate

Studies on the Interaction of DNA and Ru(1,10-phenanthroline)_2.(dipyrido[3,2.a:2.,3′-c]phenazine)^(2.)

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wuhan university Journal of Natural {2. 1999年第4期4卷 482-486页

作者： LING Lian sheng, HE Zhi ke,ZHANG Ping yi,ZENG Yun e college of chemistry and Enviromental Science,{2., wuhan, 430072,china

The interaction of Ru(phen) 2.dppz) 2. (phen=1,10 phenanthroline,dppz=dipyrido[3,2. a:2.,3′ c]phenazine) and the calfthymus DNA were studied with fluorescence and ultraviolet visible absorption spectroscopy. The ultraviolet visible absorption spectrum of Ru(phen) 2.dppz) 2. , calfthymus DNA, and their interaction demonstrated that Ru(phen) 2.dppz) 2. intercalated into the double helix of DNA via the ligand dppz. Fe(CN) 6 4- and NaCl can not quench the fluorescence of Ru(phen) 2.dppz) 2. DNA, which was in agreement with the intercalation mode.

关键词： Ru(phen) 2.dppz) 2. DNA absorption spectroscopy fluorescence intercalate binding

SO_4^(2.)/TiO_2.MoO_3催化剂催化合成缩醛(酮)

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石油化工 2003年第11期32卷 944-948页

作者：杨水金白爱民孙聚堂湖北师范学院化学与环境工程系湖北黄石435002 武汉大学化学与分子科学学院湖北武汉430072

以固体超强酸SO42./TiO2.MoO3为催化剂,对以环己酮、丁酮、正丁醛、异丁醛和乙二醇、丙二醇为原料合成缩醛(酮)的反应条件进行了研究。实验表明,固体超强酸SO42./TiO2.MoO3是合成缩醛(酮)的良好催化剂,较系统地研究了醛(酮)/醇摩尔比、... 详细信息

以固体超强酸SO42./TiO2.MoO3为催化剂,对以环己酮、丁酮、正丁醛、异丁醛和乙二醇、丙二醇为原料合成缩醛(酮)的反应条件进行了研究。实验表明,固体超强酸SO42./TiO2.MoO3是合成缩醛(酮)的良好催化剂,较系统地研究了醛(酮)/醇摩尔比、催化剂用量、反应时间诸因素对产品收率的影响。最佳反应条件为:n(醇)/n(酮或醛)=1.3/1或1.5/1,催化剂用量为反应物料总质量的0.2.％～1.5％,反应时间为1.0 h。在最佳反应条件下,环己酮-乙二醇缩酮的收率为82.7％,环己酮-1,2.丙二醇缩酮的收率为83.4％,丁酮-乙二醇缩酮的收率为74.1％,丁酮-1,2.丙二醇缩酮的收率为94.9％,丁醛-乙二醇缩醛的收率为68.1％,丁醛-1,2.丙二醇缩醛的收率为87.5％,异丁醛-乙二醇缩醛的收率为70.7％,异丁醛-1,2.丙二醇缩醛的收率为82.5％。

关键词：缩醛缩酮 SO4^2./TiO2.MoO3环己酮丁酮正丁醛异丁醛乙二醇催化合成

Monitoring the Growth Rate of HAp Crystal on the Surface of Ti/TiO_2.in SCS by a Quartz Crystal Microbalance

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生物医学工程学杂志 2005年第S1期22卷 55-56页

作者： Zhi-Hong ZHU~1 Xin-Yu SHEN~1 Peng WAN~1 Shan-Shan LIU~1 Hua TONG~(1,2.) Ji-Ming HU~11(Institute of Analytical and Biomedical Science, Collage of chemistry and molecular Science,{2. university, wuhan 430072,china)2.Center of Nano-sciences and Nano-Technology Reseach, wuhan university, wuhan 430072,china)

Synthesis and Herbicidal Activity of 2.Benzoyliminopyrimido[3,2.b]-1 2.4-Thiadiazolines

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Chinese Chemical Letters 1998年第8期9卷 721-722页

作者： Si Jia XUE Dong Yun TAN Chang You SUN(Department of chemistry, Central china Normal {2., wuhan 430079)(wuhan Food Industry college, wuhan 43002.) wuhan Food Ind Coll Wuhan 430022 Peoples R China Cent china Normal Univ Dept Chem Wuhan 430079 Peoples R China

A series of 2.benzoyliminopyrimido[3,2.b]-1,2.4-thiadiazolines were synthesized and tested for herbicidal effects. All of them are new compounds and their structures were confirmed by (HNMR)-H-1, IR, MS. The prelimina... 详细信息

关键词： pyrimido[3,2.b]-1,2.4-thiadiazoline herbicidal activity synthesis

Inhibitory effect of picroside Ⅱ on hepatocyte apoptosis

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Acta Pharmacologica Sinica 2005年第6期26卷 729-736页

作者： Hua GAO, Ya-wei ZHOU~2.college of chemistry and {2. Engineering, Peking university, Beijing 100871, china college of chemistry and {2. Engineering Peking University Beijing China

Aim: To investigate the influence of picroside on hepatocyte apoptosis and its mechanism. Methods: Morphological changes and quantification of apoptotic cells were determined under transmission electron microscopy and flow cytometry respectively. DNA fragmentation was visualized by agarose gel electrophoresis. Semi-quantitative reverse transcription-PCR (RT-PCR) was used to analyze the expression of bcl-2.and bax genes. The content of manganese-superoxide dismutase (SOD) in liver mitochondria was detected by the Marland method. The content of malonic aldehyde (MDA) and the protein level in liver tissue were determined by thiobarbituric acid colorimetry and Lowry method. Results: Picroside decreased the levels of alanine aminotransferase and aspartate aminotransferase in the serum resulting from acute-liver injured mice induced with D-GalN and LPS; it also reduced the content of MDA, and thus, enhanced the activity of SOD. Picroside 0 mg/kg was found to protect hepatocytes against apoptosis in a dose-dependent manner; it up-regulated the expression of bcl-2.genes, thus increased the bcl-2.bax ratio. Conclusion: Picroside 鈪an protect hepatocytes against injury and prevent hepatocytes from apoptosis. It might by upregulating the bcl-2.gene expression and antioxidation.

关键词： picroside Ⅱ hepatocytes apoptosis oxidative stress bcl-2.genes bax genes

Synthesis of Cyclic Core Dendritic Polymer and Its Usage as a Vector for Transferring Foreign DNA into Human Cells

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Chinese Chemical Letters 1998年第7期9卷 635-638页

作者： Bo DU Rong Jia ZHOU Ren Xi ZHUO (Laboratory of Biomedical Polymer Materials of the State Education Commission of china, Department of chemistry )(Department of Genetic, {2. of Life sciences,wuhan university, wuhan 430072) wuhan Univ State Educat Commiss China Lab Biomed Polymer Mat Dept Chem Wuhan 430072 Peoples R China wuhan Univ Coll Life Sci Dept Genet Wuhan 430072 Peoples R China

Cyclic core dendritic polymer is a new type of synthetic polymer. We describe the first synthesis of a serial dendrimers with a core of 1,4,7,10-tetraazacyclododecane. The ability of generation 4 of dendrimers to function as an effective gene delivery vectors was also investigated. Results from fluorescent in situ hybridization (FISH) show that the dendrimer has good affinity to both DNA and human small intestine cancer metastatic (HICMA) cells. the LacZ gene was highly expressed in the cells.

关键词： dendrimer 1,4,7,10-tetraazacyclododecane Michael addition transfection DNA

Conformation-activity studies on the interaction of berberine with acetylcholinesterase:Physical chemistry approach

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Progress in Natural Science:Materials International 2009年第12期19卷 1721-1725页

作者： Jin Xiang~(a,1),Changping Yu~(b,1),Fang Yang~c,Ling Yang~a,Hong Ding~(a,*) a college of Pharmacy,wuhan {2.,wuhan 430072,china b Department of Respiratory Diseases,Renmin Hospital,wuhan {2.,wuhan 430060,china c Department of Physiology,college of Basic Medicine,wuhan {2.,wuhan 430071,china [a]college of Pharmacy Wuhan University Wuhan 430072 China [b]Department of Respiratory Diseases Renmin Hospital Wuhan University Wuhan 430060 China [c]Department of Physiology College of Basic Medicine Wuhan University Wuhan 430071 China

Berberine has been reported as an acetylcholinesterase(AChE) *** significantly low cytotoxicity,berberine will be developed for the clinical treatment of Alzheimer disease(AD) with higher efficacy and fewer side *** work investigated the structure change events of AChE that occur during the interaction with berberine by isothermal titration calorimetry(ITC),fluorescence titration, and circular dichroism(CD).The results show that the binding of berberine to AChE is mainly driven by a favorable entropy increase with a less weak *** causes a loss in enzymatic activity at a concentration much below the concentration which gradually exposed the tryptophan residues to a more hydrophilic environment and unfolded the protein,which indicates that the inhibition of AChE with berberine includes the main contributions of interaction and minor conformation change of the protein induced by the alkaloid.

关键词： Acetylcholinesterase Berberine Interaction Isothermal titration calorimetry Spectroscopy

Inositol 1,4,5-trisphosphate 3-kinases:functions and regulations

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Cell Research 2005年第2期15卷 83-91页

作者： Hui Jun XIA*, Guang YANGKey Laboratory of MOE for Plant Developmental Biology, college of Life sciences,{2., wuhan, hubei 430072,china KeyLaboratoryofMOEforPlantDevelopmentalBiology CollegeofLifeSciencesWuhanUniversityWuhanHubei430072China

Inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase/IP3K) plays an important role in signal transduction in animal cellsby phosphorylating inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5-tetrakisphosphate (IP4). Both IP3 and IP4 arecritical second messengers which regulate calcium (Ca2.) homeostasis. Mammalian IP3Ks are involved in many biologicalprocesses, including brain development, memory, learning and so on. It is widely reported that Ca2. is a canonicalsecond messenger in higher plants. Therefore, plant IP3K should also play a crucial role in plant development. Recently,we reported the identification of plant IP3K gene (AtIpk2./AtIP3K) from Arabidopsis thaliana and its ***, we summarize the molecular cloning, biochemical properties and biological functions of IP3Ks from animal, yeastand plant. This review also discusses potential functions of IP3Ks in signaling crosstalk, inositol phosphate metabolism,gene transcriptional control and so on.

关键词： inositol 1 4 5-trisphosphate 3-kinase (IP3 3-kinase/IP3K) inositol polyphosphate kinase (Ipk) inositol phos-phate multikinase (Ipmk) calcium (Ca2.) signal transduction