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Neodymium cations Nd^(3+x) were transported to the interior of Euglena gracilis 2.7

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Chinese Science Bulletin 2000年第7期45卷 585-592页

作者： KANG Lin, SHEN Zhiqiang & JIN Chengzhi Institute of Genetics, State key Laboratory of Genetic Engineering, fudan university, shanghai 2.0433, China Research {2. of analysis and measurement, fudan university, shanghai 2.0433, China Correspondence should be addressed to Jin Chengzhi

Euglena gracilis 2.7, a unicellular green alga, demonstrated remarkable ability to transport Nd3+ to the cell compartments. For a given amount of Nd3+ and cells, the results of ICP-AES indicated that the cellular uptake of Nd3+ was independent of Nd3+ concentration in thebulk solution. The average uptake of Nd3+ per celi (mMd) is proportional to a parameter ξ-theratio of neodymium content to the celi counts of the system. A novel approach for probing cellular neodymium by tetraiodotetra chlorofluorescein (I4TCF) has been devised. Data derived from the cryosections of !4TCF-Nd3+ stained cells and EDAX of the fast freezing ultrathin cryosections indicate that Nd3+ is distributed over the celi compartments. Chloroplasts are the major compartments as the residence of Nd3+ in the alga. The transport should be against a concentration gradient of Nd3+ on the order of five, even higher. it is proposed that a calcium ion channel would play an important role in the Nd3+ transportation.

关键词： neodymium cations (Nd3+) Euglena gracilis EDAX I4TCF.

Field measurement and analysis of turbulence coherence for Typhoon Nuri at Macao Friendship Bridge

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Science China(Technological Sciences) 2010年第10期53卷 2647-2657页

作者： SONG LiLi1, PANG JiaBin2. JIANG ChengLin3, HUANG HaoHui3 & QIN Peng3 1 Guangzhou Institute of Tropical and Marine Meteorology, CMA, Guangzhou 510080, China 2.Tongji university, shanghai 2.0092. China 3 Guangdong Climate {2., Guangzhou 510080, China 1. Guangzhou Institute of Tropical and Marine Meteorology CMA Guangzhou 510080 China2. Tongji University Shanghai 200092 China3. Guangdong Climate Centre Guangzhou 510080 China

For the purpose of investigating the turbulent and spatial coherent characteristics of strong wind during typhoon landing period,two 3-dimensional ultrasonic anemometer stations were set up 30 m horizontally apart on the Macao Friendship Bridge to caputure the turbulent wind velocities of Typhoon Nuri. Based on the reliable and representative field measured data,the mean wind speed and direction,turbulence intensity,turbulence integral scale,turbulence power spectra,spatial correlation coefficient and coherence function were statistically evaluated. The field measurement analysis have presented the following results: 1) Two anemometer stations provided consistent results. The mean wind speed variation in time domain presented typical M-shape curves. The strong wind (10-minute mean wind speed higher than 8th grade in Beaufort wind scale) direction changed in a big range up to 12.-degrees-angle,indicating the field measurements scoped over the typhoon landing period. 2. The ratio of the longitudinal,lateral and vertical turbulence intensities of the strong wind in the typhoon eye wall region was 1:0.96:0.36. Compared with the code defined ratio 1:0.88:0.5,the lateral component was larger and the vertical component was smaller. 3) The value of integral scale increased when the eye wall of Typhoon Nuri passed over the field measurement site. Before the center of Typhoon Nuri arrived,the integral scale of the strong typhoon wind was about twice compared with that for the non-typhoon wind. 4) The spatial correlation of the turbulent wind,coherence function curve and the decay factor had significant differences at different times during the typhoon process. In the eye wall of the typhoon,the horizontal spatial correlation was relatively strong and horizontally spatial correlation spectrum decayed slower with frequency increase. The minimum regressed coefficient C in coherence function model was 4.67,which is lower than the code defined low limit. The maximum decay factor w

关键词： typhoon field measurement and analysis turbulence characteristics coherence spectral function

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油/气管道检测机器人

上海交通大学学报 2003年第11期37卷 1653-1656页

作者：徐小云颜国正闫小雪上海交通大学信息检测技术及仪器系上海200030 上海翱腾科技有限公司上海200030

在轮式和履带式机器人的基础上,设计开发了一种新的管内移动机器人.机器人的3组驱动轮沿圆周方向12.°均布,在轴向截面内,前后两组驱动轮布置在同一组平行四边形机构上,驱动电动机通过蜗轮蜗杆副驱动3组驱动轮,调节电动机通过滚珠丝杠... 详细信息

在轮式和履带式机器人的基础上,设计开发了一种新的管内移动机器人.机器人的3组驱动轮沿圆周方向12.°均布,在轴向截面内,前后两组驱动轮布置在同一组平行四边形机构上,驱动电动机通过蜗轮蜗杆副驱动3组驱动轮,调节电动机通过滚珠丝杠螺母副和压力传感器使3组驱动轮始终以稳定的正压力紧贴在管道内壁,使机器人具有充裕并且稳定的牵引力.该机器人机构紧凑,工作可靠,适用于管径为 400～650mm的管道.

关键词：管道机器人平行四边形机构管道检测

Studies on solid state reactions of coordination compounds——XLⅦ.Solid state syntheses and crystal structures of cluster compounds {Cu_3MoS_3I}(PPh_3)_3S and {Cu_3WS_3Br} (PPh_3)_3S

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Chinese Journal of Chemistry 1993年第1期11卷 21-29页

作者： LANG,Jian-Ping ZHU,Hui-Zhen XIN,Xin-Quan Department of Chemistry,Nanjing university,Nanjing 2.0008CHEN,Ming-Qin LIU,Kun ZHENG,Pei-Ju Center of analysis and {2.,fudan university,shanghai 2.0433

Reactions of [NH_4]_2.MS_4](M=Mo,W),CuX(X=Br,I)and PPh_3 in the solid state produced four mixed-metal sulfur containing clusters{Cu_3MS_3X}(PPh_3)_3S(M=Mo,W;X=Br,I),two of which (1:M=Mo,X=I;2.M=W,X=Br)were structurally *** of 1 and 2.are triclinic, space group P(1:a=11.895(3),b=13.107(1),c=2..473(2.,α=74.95(6),β=84.87(8),γ=64.2.(7)°, Z=2.V=2.76.1 ,Rw=0.064 for 6443 observed reflections.2.α=11.876 (1),b=13.065 (2.,c= 2..32.(2.,α=74.95(1),β=85.39(1),γ=64.09(1)°,Z=2.V=2.37.3,R_w=0.055 for 5303 observed reflections).The results of the structure determination showed that the central units of the two cubane- like cluster compounds are composed of four metal atoms and four non-metal atoms situated at alter- nate *** differences of cubane-like cluster compounds obtained from solid state reactions and from solution reactions are discussed.

关键词： Solid state syntheses and crystal structures of cluster compounds and Cu3WS3Br PPh3 WS Br XL

Recent developments of nanoparticle-based enrichment methods for mass spectrometric analysis in proteomics

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Science China Chemistry 2010年第4期53卷 695-703页

作者： ZHANG LiJuan, LU HaoJie & YANG PengYuan Department of Chemistry and Institutes of Biomedical Sciences, fudan university, shanghai 2.0433, China 1. Department of Chemistry and Institutes of Biomedical Sciences Fudan University Shanghai 200433 China

In proteome research, rapid and effective separation strategies are essential for successful protein identification due to the broad dynamic range of proteins in biological samples. Some important proteins are often expressed in ultra low abundance, thus making the pre-concentration procedure before mass spectrometric analysis prerequisite. The main purpose of enrichment is to isolate target molecules from complex mixtures to reduce sample complexity and facilitate the subsequent analyzing steps. The introduction of nanoparticles into this field has accelerated the development of enrichment methods. In this review, we mainly focus on recent developments of using different nanomaterials for pre-concentration of low-abundance peptides/ proteins, including those containing post-translational modifications, such as phosphorylation and glycosylation, prior to mass spectrometric analysis.

关键词： enrichment nanoparticles mass spectrometry proteomics phosphorylation glycosylation

Bacterial Type Ⅰ Glutamine Synthetase of the Rifamycin SV Producing Actinomycete,Amycolatopsis mediterranei U32.is the Only Enzyme Responsible for Glutamine Synthesis under Physiological Conditions

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Acta Biochimica et Biophysica Sinica 2006年第12期 821-830页

作者： Wen-Tao PENG~(1&) Jin WANG~1 Ting WU~2.Jian-Qiang HUANG~1 Jui-Shen CHIAO~1 Guo-Ping ZHAO~(1,3,4) 1 Laboratory of Molecular Microbiology,Institute of Plant Physiology and Ecology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 2.0032.China 2.Department of Medicinal Chemistry,School of Pharmacy,{2. university,shanghai 2.0032.China 3 State Key Laboratory of Genetic Engineering/Department of Microbiology,School of Life Science,{2. university,shanghai 2.0433,China 4 Laboratory of Health and Disease Genomics,Chinese National Haman Genome Center at shanghai,shanghai 2.12.3,China

The structural gene for glutamine synthetase,glnA,from Amycolatopsis mediterranei U32.wascloned via screening a genomic library using the analog gene from Streptomyces *** clone wasfunctionally verified by complementing for glutamine requirement of an Escherichia coli glnA null mutantunder the control of a lac *** analysis showed an open reading frame encoding a protein of466 amino acid *** deduced amino acid sequence bears significant homologies to other bacterialtype Ⅰ glutamine synthetases,specifically,71% and 72. identical to the enzymes of *** and Myco-bacterium tuberculosis,*** of this glnA gene in *** U32.led to glutamineauxotrophy with no detectable glutamine synthetase activity in *** contrast,the cloned glnA+gene cancomplement for both phenotypes in *** thus suggested that in *** U32.the glnA geneencoding glutamine synthetase is uniquely responsible for in vivo glutamine synthesis under our laboratorydefined physiological conditions.

关键词： glnA glutamine synthetase Amycolatopsis mediterranei rifamycin SV

比较金融制度分析与中国金融制度改革的路径选择

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思想战线 2003年第3期29卷 23-27页

作者：蒋冠张露云南大学经济学院云南昆明650091 复旦大学经济学院上海200433

不同国家金融制度的形成,有其特定的社会传统和演进路径,在经济发展中的功能和作用也各有优势。比较金融制度分析,就是研究不同类型金融系统的特点,提供最优的金融系统参照。我国金融制度改革的路径选择,需要借鉴他山之石,并结合具体的... 详细信息

不同国家金融制度的形成,有其特定的社会传统和演进路径,在经济发展中的功能和作用也各有优势。比较金融制度分析,就是研究不同类型金融系统的特点,提供最优的金融系统参照。我国金融制度改革的路径选择,需要借鉴他山之石,并结合具体的制度环境以及其中的问题,进行全面的权衡,最终建立一个按照市场规则运行的开放和透明的金融体系,支持社会经济的可持续发展。

关键词：比较金融制度金融改革路径选择

Prognostic role of diabetes mellitus in hepatocellular carcinoma patients after curative treatments:a meta-analysis

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 346-355页

作者： Wei-Min Wang,Yang Xu,Xin-Rong Yang,Yao-Hui Wang,Hai-Xiang Sun and Jia Fan Institute of Biomedical Sciences,fudan university,{2. 2.0032.China Key Laboratory of Carcinogenesis and Cancer Invasion,Ministry of Education,and Liver Cancer Institute,Zhongshan Hospital,fudan university,{2. 2.0032.China fudan university {2. Cancer Center,{2. 2.0032.China [a] Institute of Biomedical Sciences Fudan University Shanghai 200032 China [b] Key Laboratory of Carcinogenesis and Cancer Invasion Ministry of Education and Liver Cancer Institute Zhongshan Hospital Fudan University Shanghai 200032 China [c] fudan university shanghai Cancer Center Shanghai 200032 China

BACKGROUND:The prognostic role of diabetes mellitus (DM) coexisting with hepatocellular carcinoma (HCC) remains *** clarify its impact on survival in HCC patients after curative treatments,a meta-analysis was *** SOURCES:Eligible studies were identified through multiple search strategies in the databases PubMed (MEDLINE),EMBASE,the Cochrane Library and ACP Journal Club between January 1950 and March *** studies fulfilled the inclusion criteria,and data were aggregated comparing overall survival and recurrence-free survival in HCC patients according to DM ***:The pooled hazard ratios (HRs) estimate for overall survival was 1.34 (95% CI,1.18-1.51;P2.18;P2.66;P=0.016),while HBV-related cases were not significantly different (HR=1.2.;95% CI,0.69-2.40;P=0.182..Meanwhile,the coexistence of DM impaired overall survival in HCC patients with a small tumor burden (HR=1.63;95% CI,1.2.-2.12.P<0.0001).CONCLUSION:HCC patients with coexisting DM have a shorter survival time and a higher risk for tumor recurrence after curative treatments,while the precise value should be defined in more clinical trials with consistent methodology,especially prospective studies.

关键词： hepatocellular carcinoma diabetes mellitus prognostic factors survival meta-analysis

高效液相色谱法测定依托度酸及其在大鼠的药动学(英文)

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中国新药与临床杂志 2002年第4期21卷 193-196页

作者：石劲敏许长江段更利李端复旦大学药学院药理教研室上海200032 复旦大学药学院药物分析教研室上海200032

目的 :建立测定依托度酸浓度的高效液相色谱法并研究该药在大鼠体内的药动学。方法 :血浆 2.0 0 μL加入 1 0 0mg·L-1 内标 (奥沙普秦) 50 μL ,酸化后用乙酸乙酯∶正己烷 (1∶1 9)提取。采用C1 8分析柱 ,以乙腈∶水∶三乙胺 (1 6∶84... 详细信息

目的 :建立测定依托度酸浓度的高效液相色谱法并研究该药在大鼠体内的药动学。方法 :血浆 2.0 0 μL加入 1 0 0mg·L-1 内标 (奥沙普秦) 50 μL ,酸化后用乙酸乙酯∶正己烷 (1∶1 9)提取。采用C1 8分析柱 ,以乙腈∶水∶三乙胺 (1 6∶84∶0 .0 0 2.)为流动相 ,流速为 1 .0mL·min-1 ;检测波长 2.80nm。大鼠按依托度酸 2.0mg·kg-1 剂量灌胃 ,给药前及给药后 5 ,1 0 ,2.0 ,30min和 1 ,3,6 ,1 2.,2.4 ,48,72.采血 ,测定血浆药物浓度。绘制血药浓度 -时间曲线图并计算主要药动学参数。结果 :依托度酸与内标物的保留时间分别为 5 .2.8min和 6 .80min ;依托度酸浓度在1 .0～ 1 0 0 .0mg·L-1 范围内与依托度酸/内标物的峰面积比呈良好线性关系 ,回归方程为 :Y =0 .0 32.2.7C +0 .0 0 1 2.8,r =0 .9999,n =7。本法高、中、低浓度的绝对回收率为 73 .4 %～ 80 .5 % ,方法回收率在 97.3%～1 0 6.0 % ,日内RSD 2.8h± 0 .1 3h ;Cmax=69mg·L-1 ± 5mg·L-1 ;T1 /2.=1 6 .9h± 2..1h ;AUC =2.31 4mg·h·L-1 ±32.8mg·h·L-1 。结论 :本法快捷、灵敏、准确、重复性好 ,适用于依托度酸血药浓度测定及药动学研究。

关键词：高效液相色谱法测定依托度酸大鼠药动学血药浓度