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Study on the stannous halides promoted addition of 1,1,1-trichloro-2.2.2.trifluoroethane to aldehydes and the chemical transformation thereof

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chinese Journal of Chemistry 1994年第2期12卷 183-189页

作者： HU, Chang-Ming CHEN, Jianshanghai Institute of Organic Chemistry, chinese academy of sciences,{2., china shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 China

Promoted by stannous salts, 1,1,1-trichloro-2.2.2.trifluoroethane reacts readily with aliphatic, aromatic, and α, β-unsaturated aldehydes giving the corresponding alcohols bearing a CF3CCl2.moiety in good to excelle... 详细信息

关键词： 1,1,1-Trichloro-2.2.2.trifluoroethane aldehydes stannous halides addition Jones oxidation ketones.

N-Acetyltransferase 2.gene polymorphism in a group of senile dementia patients in shanghai suburb

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Acta Pharmacologica Sinica 2004年第9期 10-15页

作者： Wei-chao GUO, Guo-fang LIN, Yong-lin ZHA, Ke-jian LOU, Qing-wen MA, Jian-hua SHENInstitute of Plant Physiology and Ecology, shanghai Institutes for Life sciences,{2., shanghai 2.002. District Mental Health Center, Qingpu, shanghai 2.1700, china

AIM: To investigate the possible association of hereditary polymorphism of N-acetyltransferase 2.(NAT2. gene with the susceptibility towards senile dementia in farmer population of shanghai suburb. METHODS: NAT2.gene genotyping was performed at 7 major polymorphic loci (G191A, C2.2., T341C, C481T, G590A, A803G, and G857A) with a polymerase chain reaction-based restriction fragment length polymorphism based procedure in 2.groups of farmer subjects in shanghai suburb. A group of 51 diagnosed dementia patients [comprising 2. sporadic Alzheimer disease (AD) patients and 2. sporadic vascular dementia (VD) patients] and a group of 112.healthy individuals were in the same area. RESULTS: The homogenous rapid genotypes (R/R, including*4/*4, *13 /*13, and *4/*13) was found over-present in both groups of patients, compared with healthy individuals, for all farmer dementia patients, 52.9 % vs 33.0 %, P=0.016, OR (95 % CI): 2.2.(1.16-4.48); for AD group only, 51.7 % vs 33.0 %, P=0.063, OR (95 % CI): 2.18 (0.95-4.97);

关键词： N-acetyltransferases 2 genotype Alzheimer disease polymorphism (genetics)

Recent progress in neurodegenerative disorder research in china

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Science china(Life sciences) 2010年第3期53卷 348-355页

作者： ZHOU JiaWei Institute of Neuroscience,shanghai Institutes for Biological sciences,{2. academy of sciences,shanghai,20003. 1. Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Neurodegenerative disorders,including Alzheimer's disease(AD) and Parkinson's disease(PD),are common disorders of the central nervous system among aging *** the last 10 years insights concerning the etiology,diagnosis and pathogenesis of these diseases have come from research carried out by chinese *** findings include the description of chinese patients with autosomal recessive early-onset PD,the function of the tau protein,molecular mechanisms underlying protein aggregation,and the identification of biomarkers for AD diagnosis and molecules/compounds with potential neuroprotective activities.

关键词： neurodegenerative disorders pathogenesis diagnosis neuroprotection Alzheimer’s disease Parkinson’s disease

Detection of Tumor Marker CA12. in Ovarian Carcinoma Using Quantum Dots

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 681-686页

作者： Hui-Zhi WANG, Hai-Yan WANG, Ru-Qiang LIANG, and Kang-Cheng RUAN* Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological sciences,{2. academy of sciences, Graduate School of the chinese academy of sciences, shanghai 20003., china Key Laboratory of Proteomics Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesGraduate School of the Chinese Academy of SciencesShanghai 200031China

Semiconductor quantum dots (QDs) offer several advantages over organic dyes in fluores- cence-imaging applications, such as higher quantum yield, exceptional photostability, and a narrow, tunable, and symmetric emission spectrum. To explore whether QDs could specifically and effectively label tumor markers and be used in immunohistochemistry as a novel type of fluorescent probe, we used quantum dots with maximum emission wavelength 605 nm (QD605) to detect the ovarian carcinoma marker CA12. in specimens of different types (fixed cells, tissue sections, and xenograft piece). Additionally, we compared the photostability of QD signals with that of a conventional organic dye, FITC. All labeling signals of QDs were found to be more specific and brighter than those of FITC. Moreover, the QDs exhibited exceptional photostability during continuous illumination for 1 h by a high-intensity laser (Ar laser power 100 mW) at 488 nm, while the FITC signals faded very quickly and became undetectable after 2. min of illumination. These results indicate that QD-based probes can offer substantial advantages over existing fluorophores in many applications, and can be used effectively in immunohistochemistry as a novel class of fluorescent probes.

关键词： quantum dots detection of tumor marker CA12. ovarian carcinoma

Synapse formation and remodeling

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Science china(Life sciences) 2010年第3期53卷 315-321页

作者： LUO ZhenGe Institute of Neuroscience and State Key Laboratory of Neuroscience,shanghai Institutes for Biological sciences,{2. academy of sciences,shanghai 20003.,china 1. Institute of Neuroscience and State Key Laboratory of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Synapses are specialized structures that mediate information flow between neurons and target cells,and thus are the basis for neuronal system to execute various functions,including learning and *** are around 1011 neurons in the human brain,with each neuron receiving thousands of synaptic inputs,either excitatory or inhibitory.A synapse is an asymmetric structure that is composed of pre-synaptic axon terminals,synaptic cleft,and postsynaptic *** formation involves a number of cell adhesion molecules,extracellular factors,and intracellular signaling or structural *** the establishment of synaptic connections,synapses undergo structural or functional changes,known as synaptic plasticity which is believed to be regulated by neuronal activity and a variety of secreted *** review summarizes recent progress in the field of synapse development,with particular emphasis on the work carried out in china during the past 10 years(1999-2.09).

关键词： synapse development neuronal activity synaptic plasticity

Nucleic Acid Binding Activity of Pns6 Encoded by Genome Segment 6 of Rice Ragged Stunt Oryzavirus

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 457-466页

作者： Chao-Gang SHAO#, Hui-Juan Lü#, Jian-Hua WU, and Zu-Xun GONG* Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, shanghai Institutes of Life sciences,{2., Graduate School of the{2., shanghai 20003., china Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes of Life Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai 200031 China

The ORF of genome segment 6 (S6) of rice ragged stunt oryzavirus (RRSV) Philippines isolate was cloned and sequenced based on the S6 sequence of the Thailand isolate. Pns6, the 71 kD product of S6 expressed in E. coli, was demonstrated to be a viral non-structural protein of RRSV by Western blotting. The gel mobility shift assays showed that Pns6 had nucleic acid binding activity. Pns6 could interact with single- and double-stranded forms of DNA and RNA, showing a preference for single-stranded nucleic acid and a slight preference for RRSV ssRNA over the rice ssRNA, as demonstrated by both competition and displacement assays. The binding of Pns6 to nucleic acids is strong and sequence non-specific. By using five truncated derivatives of Pns6, it was found that the basic region from amino acid 2.1 to 2.3 of Pns6 was the unique nucleic acid binding domain. Subcellular fractionation of leaf tissues of RRSV-infected rice plants and subsequent Western blotting had shown that Pns6 accumulated predominately in the cytoplasmic membrane fraction. The possible role of RRSV Pns6 in virus replication and assembly is discussed.

关键词： rice ragged stunt oryzavirus (RRSV) nucleic acid binding non-structural protein S6

Synthesis and crystal structure of SrNi_xV_(6-x)O_(11)

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chinese Science Bulletin 1997年第10期42卷 850-855页

作者： LIU Hongchao, GUO Changlin and TU Hengyongshanghai Institute of Ceramics, chinese academy of sciences, shanghai 2.0050, china shanghai Institute of Ceramics Chinese Academy of Sciences Shanghai China

THE AV6O11（A,alkaline or alkaline earth metals）-type compounds are the only phases

关键词： SrNixV6-xO11 crystal structure X-ray powder diffraction Rietveld method bond-valence method.

mad-overexpression down regulates the malignant growth and p53 mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells

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Cell Research 1999年第1期9卷 51-59页

作者： ZHAO HUA YONG HUA XU (shanghai Institute of Cell Biology, chinese academy of sciences, 32. yue-yang Road,{2., china) 1shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Mad protein has been shown as an antagonist of cMyc protein in some cell lines. The effect of Mad protein to the malignant phenotype of human hepatoma BEL7404 cell line was investigated experimentally. An eukarryotic vector pCDNA Ⅲ containing full ORF fragmentof mad cDNA was transfected into targeted cells. Under G418 selection, stable Mad-overexpressed cells were *** on the effect of Mad over-expression in cell proliferation and cell cycle revealed that cell morphology of the Mad-overexpressed BEL-7404-M1 cells was significantly different from the parent and control vector transfected cells. DNA synthesis, cell proliferation and anchorage-independent growth in soft-agar of the madtransfected cel1s were partially inhibited in comparison to control *** Cytometry analysis indicated that mad over-expression might block more transfectant cells at G0/G1 phase, resulting in the retardation of cell proliferation. RT-PCR detected a marked inhibition of the expression of cdc2.A, an important regulator gene of G0/G1to S phase in cell cycle. It was also found that Mad protein overexpression could greatly suppress p53-mediated apoptosis in BEL-7404-M1 cells in the absence of ***, Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL7404 *** and regulation of cell growth and apoptosis

关键词： Mad c-Myc cell growth apoptosis human hepatoma cells

Photoinduced Phenomena in Amorphous Ge-As-S System

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chinese Journal of Lasers 2002年第1期11卷 40-45页

作者： LIU Qiming GU Donghong HUANG Mingju GAN Fuxi (shanghai Institute of Optics and Fine Mechanics, The chinese academy of sciences, shanghai 2.1800, china) shanghai Institute of Optics and Fine Mechanics The Chinese Academy of Sciences 上海 201800

X-ray diffraction and reflectivity as well as transmittance on Ge-As-S bulk and thin films were performed. It was observed photodarkening in Ge-As-S thin films with band gap light illumination from an argon laser of 514.5 nm wavelength and shifting the optical transmittance edge to shorter wavelength, the magnitude of which increases with the increase of the intensity of illumination light. In the Ge-As-S bulk, however, it was not observed photodarkening. Photodarkening in Ge-As-S system was discussed.

关键词： Ge-As-S bulk and thin film band gap light illumination photodarkening