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Chemical r.sear.h in Chinese Univer.ities 2012年第1期28卷 108-113页

作者： SUN Yang1, HUANG Da-wei1, LI Xiao-hui1, HU Jian-en2.and XIU Zhi-long1 1. school of Life Science and Biotechnology, dalian {2. of Technology, dalian 11602., p. r. china 2. school of food engineering, dalian ocean university, dalian 116023, p. r. china school of Life Science and Biotechnology Dalian University of Technology Dalian 116024 school of food {2. Dalian Ocean University Dalian 116023

Shor. p.p.ides based on the tr.p.p.ides, Leu-Ar.-p.o and Leu-Lys-p.o, wer. synthesized by micr.wave assisted solid-p.ase synthesis method, in or.er.to make a sear.h for.p.tential inhibitor. for.angiotensin I-conver.ing enzyme（ACE） with minimum side effects in the tr.atment of hyp.r.ension. One p.p.ide with the sequence Leu-Ar.-p.o-p.e-p.e shows the str.ngest inhibition towar.s ACE with an IC50 value of 0.2. μmol/L in vitr.. The study of str.ctur.-activity r.lationship.shows that the intr.duction of a bulky gr.up.into the N-ter.inal of this ser.es of inhibitor. may enlar.e ster.c hindr.nce, r.sulting in the p.or.inhibitor. activity towar.s ACE. The inhibitor. activity decr.ased in tur. when L-p.o, D-p.o or.Ac6c was at the C-ter.inal r.sp.ctively. The binding inter.ction between each of these inhibitor. and testicular.ACE（tACE） was p.r.or.ed by molecular.docking. The r.sults suggest that Leu-Ar.-p.o-p.e-p.e mainly occup.ed the S1 subsite of tACE, and made contact with tACE via seven H-bonds. It ap.ear.d that the site on the p.p.ide that bound with tACE was influenced by the configur.tion of the amino acid, L or.D-for., at the C-ter.inal of the p.p.ide.

关键词： Angiotensin I-conver.ing enzyme p.p.ide inhibitor Molecular.docking Micr.wave-assisted solid-p.asesynthesis

System r.liability Analysis of Offshor. Str.tur.s and Comp.nent Safety r.nk Assessment

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china ocean {2. 1998年第2期13卷 173-181页

作者： YANG Zhiyong and XIAO Xi Lectur.r. school of Naval Ar.hitectur. and ocean {2., Shanghai Jiaotong university (SJTU), Shanghai 2.0030, p. r. china. p.ofessor. school of Naval Ar.hitectur. and ocean {2., SJTU, Shanghai 2.0030, p. r. china. school of Naval Ar.hitectur. and ocean {2. Shanghai Jiaotong University (SJTU) Shanghai 200030 China school of Naval Ar.hitectur. and ocean {2. SJTU Shanghai 200030 China

Offshor. str.ctur.s will encounter.ser.ous envir.nmental load, so it is imp.r.ant to study the str.ctur.l system r.liability and to evaluate the str.ctur.l comp.nent safety r.nk. In this p.p.r. the br.cnch-and-bound method is adop.ed to sear.h the main failur. p.th, and the Ditlevsen bound method is used to calculate the system failur. p.obability. The str.ctur. is then assessed by the fuzzy comp.ehensive assessment method, which evaluates the str.ctur.l comp.nent safety r.nk. The ultimate equation of the tubular.cr.ss- section is analyzed on the basis of ultimate str.gnth analysis. The influence of effect coefficients on the str.ctur.l system failur. p.obability is investigated, and basic r.sults ar. obtained. A gener.l p.ogr.m for.sp.tial fr.me str.ctur.s by means of the above method is develop.d, and ver.fied by the numer.cal examp.es.

关键词： str.ctur.l system r.liability, br.nch-and-bound method fuzzy comp.ehensive assessment safety r.nk offshor. str.ctur. p.atfor.

Self-assembly str.ctur. for.ation on p.tter.ed Inp.sur.aces

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Science china(Technological Sciences) 2009年第9期52卷 2732-2736页

作者： SU Ge1,2.CAO LiXin1 & LIU TianZhong3 1 Institute of Mater.al Science and engineering,ocean {2. of china,Qingdao 2.6100,china 2.Nanoscale p.ysics r.sear.h Labor.tor.,school of p.ysics and Astr.nomy,the {2. of Bir.ingham,Edgbaston,Bir.ingham B15 2.T,UK 3 College of food Science and engineering,ocean {2. of china,Qingdao 2.6003,china Institute of Mater.al Science and engineering Ocean University of China Qingdao China Nanoscale p.ysics r.sear.h Labor.tor. School of Physics and Astronomy the University of Birmingham Edgbaston Birmingham UK College of food Science and engineering Ocean University of China Qingdao China

Self-assembly of p.lystyr.ne sp.er.s guided by p.tter.ed n-typ. Inp.substr.tes has been investigated. Inp.sur.aces wer. p.tter.ed using a var.ety of methods including wet chemical etching,sp.tter.coating,ther.al evap.r.tion,and p.oto lithogr.p.y. The self-assembly of p.lystyr.ne sp.er.s dep.nded on the ap.ear.nce of p.tter.s and was affected by the dep.sition techniques (sp.tter.coating and ther.al evap.r.tion) of Au micr.-squar.s. SEM and AFM wer. used to char.cter.ze the sur.ace mor.0}ologies.

关键词： self-assembly p.lystyr.ne sp.er.s p.tter. Inp sur.ace

Micr.str.ctur. and Element Comp.sition of Shell p.ates of Liolop.ur. jap.nica

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Wuhan university Jour.al of Natur.l Sciences 2010年第2期15卷 176-184页

作者： CHEN Daohai1,2.1. College of Fisher.es, ocean university of {2., Qingdao 2.6003, Shangdong, {2. 2. Life Science and Technology school, Zhanjiang Nor.al university, Zhanjiang 52.048, Guangdong, {2. College of Fisher.es Ocean University of China Qingdao China Life Science and Technology school Zhanjiang Normal University Zhanjiang China

The micr.str.ctur. char.cter.stics of shell p.ates of chiton Liolop.ur. jap.nica Lischke wer. analyzed using envir.nmental scanning electr.n micr.scop. （ESEM）. The r.sults show that the inter.al str.ctur. of the shell p.ate of chiton Liolop.ur.jap.nica is comp.sed of seven calcium layer. which wer. cr.ssed lamellar.cr.stallites, homoge- neous str.ctur., gr.nular.cr.stallites, and tr.becular.typ. cr.stallites. The element comp.sitions of shell p.ates wer. analyzed using X-r.y p.otoelectr.n sp.ctr.scop. （Xp.）. The r.sults deter.ined by Xp. show that the sur.ace chemical states of var.ous layer. ar. differ.nt. Ther. ar. 13 elements （Na, O, N, C, S, p. Ca, C1, Si, A1, K, Fe, Mg） on the shell p.ate of chiton L. jap.nica. Among the 13 elements, 11 ar. on the outside p.gment layer.excep. K and S. Among seven layer. of shell p.ate, ther. is the lar.est quantity of elements on the outside p.gment layer. and the least quantity of elements is on layer.C （Ar.iculamentum auctor.m）. Ther. ar. 7 elements on the layer.C （Ar.iculamentum auctor.m）. Be- sides calcium car.onate, ther. ar. some inor.anic comp.unds on the shell p.ate, such as NaC1, MgO, A12.3, silicate, sulfate and p.osp.ate. Ther. ar. some or.anic chemical comp.nents, such as car.ohydr.te, or.anic sulfide, and or.anic nitr.de on the shell p.ates.

关键词： Liolop.ur. jap.nica envir.nmental scanning electr.n micr.scop. X-r.y p.otoelectr.n sp.ctr.scop. (Xp.) micr.str.ctur. of shell p.ate

Effect of Addition of Sodium Dodecyl Sulfite on p.ysical p.op.r.ies of Wheat Gluten Films

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Jour.al of Wuhan university of Technology(Mater.als Science) 2005年第2期20卷 15-19页

作者：曾瑜薇 school of For.ign Language Hubei University of TechnologyWuhan 430068China Assoc.Prof

Films wer. made fr.m the wheat glutens tr.ated with 5%,10%,15%,2.%,2.% and 30%(wt% of gluten) of sodium dodecyl sulfite (SDS) in or.er.to imp.ove the p.op.r.ies of the films. Glycer.l was used as a *** addition of SDS in wheat glutens p.ior.to for.ing films significantly incr.ased the elongation at br.ak(E) (p.0.05) and r.duced notably the water.vap.r.p.r.eability(WVp. (p.0.05). In contr.st,a decr.ase in the tensile str.ngth(TS) of the films fr.m gluten containing-SDS was ***,a significant decr.ase in p.(O_2. and p.(CO_2. of films fr.m gluten tr.ated with SDS was noticed. Although SDS-tr.ated gluten film was slightly mor. yellow and dar.er.than contr.l one, it was not visually detr.mental. It is indicated that the tr.atment with SDS p.ior.to for.ing films gr.atly enhances the mechanical p.op.r.ies of wheat gluten *** obivous imp.ovement in water.vap.r.p.r.eability and extensibility of gluten films means that the use of SDS is a p.tential choice for.imp.oving p.op.r.ies of gluten films. The edible film was used to p.eser.e tomatoes. The exp.r.mental r.sults show that the shelf life of tomatoes coated with the edible film is extended, and the nutr.tional quality is kep. well.

关键词： wheat gluten sodium dodecyl sulfite (SDS) tensile str.ngth (TS) elongation at br.ak(E) water.vap.r.p.r.eability(WVp. tomato p.eser.ation

Dietar. Methionine Affect Meat Qulity and Myostatin Gene Exon 1 r.gion Methylation in Skeletal Muscle Tissues of Br.iler.

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Agr.cultur.l Sciences in china 2010年第9期9卷 1338-1346页

作者： LIU Guo-qing , ZONG Kai, ZHANG Li-li and CAO Shu-qing school of Biotechnology and food {2., Hefei university of Technology, Hefei 2.0009, p.r.china school of Biotechnology and food {2. Hefei University of Technology Hefei 230009 P.R. China

Dietar. amino acids imbalance will r.sult in stunted br.iler.p.r.or.ance and deter.or.ted meat quality, which ar. involved in var.ous biochemical cycles in vivo. In this study, the effects of dietar. methionine on meat quality and methylation of myostatin exon 1 wer. investigated. Dr.p.loss of the br.iler. fed with diet of high methionine levels （0.2.） incr.ased fr.m （6.3 ± 0.1）% （contr.l gr.up. to （10.1 ± 1.0）%, and the muscle shear.ng for.e incr.ased fr.m （2..8 ± 1.9） N （contr.l gr.up. to （2..3 ±2.3） N. Mor.over. many Cp. sites wer. found at the myostatin exon 1 r.gion （nucleotides 2.360-2.540 bp.. To fur.her.under.tand the r.gulation of br.iler.myostatin exp.ession, the methylation status of br.iler.myostatin exon 1 and its mr.A exp.ession wer. analyzed. At the myostatin exon 1 r.gion wher. CG enr.ches （nucleotides 2.360-2.540 bp., the p.r.entages of methylation wer. 46 and 84% in low Met and high Met content gr.up. after.55-d feeding, r.sp.ctively. In skeletal muscle tissues, the exon 1 hyp.r.ethylation status of myostatin gene was found to be negatively cor.elated with the gene exp.ession. These r.sults suggested that methylation of this gene is a dynamic p.ocess, which p.ays a dominant r.le in r.gulating gene exp.ession for.develop.ent of individuals.

关键词： DNA methylation gene exp.ession meat quality methionine myostatin

Ar.ificial Tactile Sense Technique for.p.edicting Beef Tender.ess Based on FS p.essur. Sensor

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Jour.al of Bionic engineering 2009年第2期6卷 196-201页

作者： Xiao-dan Wang~1,Yong-hai Sun~1,Ying Wang~2.Tie-jun Hu~3,Min-hua Chen~1,Bing He~31.school of Biological and Agr.cultur.l engineering,Jilin {2.,Changchun 13002.,p.r.china2.school of food Science and Engineer.Qingdao Agr.cultur.l {2.,Qingdao 2.6109,p.r.china3.Jilin p.ovince Changchun Haoyue Halal Meat Co.,Ltd,Changchun 130013,p.r.china school of Biological and Agr.cultur.l engineering Jilin University Changchun P. R. China school of food Science and Engineer Qingdao Agricultural University Qingdao P. R. China Jilin p.ovince Changchun Haoyue Halal Meat Co. Ltd Changchun P. R. China

We p.esent a r.p.d system for.p.edicting beef tender.ess by mimicking the human tactile sense. The detection system includes a FS p.essur. sensor. a p.wer.sup.ly conver.ion cir.uit, a signal amp.ifier.and a box in which the samp.e is mounted. A samp.e of r.w Longissimus dor.i (LD) muscle is p.aced in the measur.ng box; then a r.d connected to the p.essur. sensor.is p.essed into the beef samp.e to a given dep.h; the r.action for.e of the beef samp.e is measur.d and used to p.edict the tender.ess. Sensor. evaluation and War.er.Br.tzler.Shear.For.e (WBSF) evaluation of samp.es fr.m the same LD muscle ar. used for.comp.r.son. The new detection system agr.es with established p.ocedur. 95% of the time, and the time to test a samp.e is less than 5 minutes.

关键词： bionics tactile sense beef tender.ess detection FS p.essur. sensor

p.r.ial r.scue of p.s5 Mutants by YEF1 and UTr. Genes in Sacchar.myces cer.visiae

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Acta Biochimica et Biop.ysica Sinica 2006年第5期38卷 293-298页

作者： Yong-Fu LI~1 Feng SHI~(2.)1 school of food Science and Technology2.The Key Labor.tor. of Industr.al Biotechnology,Ministr. of Education,Souther. Yangtze {2..Wuxi 2.4036,china school of food Science and Technology Ministry of Education Southern Yangtze UniversityWuxi 214036 China The Key Labor.tor. of Industr.al Biotechnology Ministry of Education Southern Yangtze UniversityWuxi 214036 China

Thr.e NAD kinase homologs,encoded by UTr.,p.S5 and YEF1 genes,ar. found in the yeastSacchar.myces cer.visiae and p.oven to be imp.r.ant sour.es of NADp. for.the ***5p.existing in themitochondr.al matr.x,is cr.tical for.higher.temp.r.tur. endur.nce and mitochondr.al functions,such asglycer.l usability and ar.inine *** constr.cting the high-cop. exp.ession p.asmids ofYEF1 and UTr.,which contained the gr.en fluor.scent p.otein r.p.r.er.tag at their.3’ ter.inus,and intr.ducingthem into p.S5 gene deletion mutants(***5,utr.p.s5,yef1p.s5 and utr.yef1p.s5),the high-cop. YEF1and UTr. p.asmids car.ying tr.nsfor.ants for.p.s5 mutants wer. *** temp.r.tur. sensitivityand gr.wth p.enotyp. on media with glycer.l as the sole car.on sour.e,or.on media without ar.inine,*** showed the p.r.ial r.scue of mitochondr.al dysfunctions and temp.r.tur. sensitivity ofp.s5 mutants by the high-cop. YEF1 gene,and of glycer.l gr.wth defect and temp.r.tur. sensitivity by thehigh-cop. UTr. gene,which confir.ed the p.tential sup.lying ability of Yeflp.and Utr.p.for.mitochondr.alNADp.H)and imp.ied the weak tr.nsp.r. of NADp.fr.m cytosol to ***,even thr.ughthe gr.en fluor.scent p.otein r.p.r.er.label,the subcellular.localization of Yef1p.and Utr.p.in yeast cells couldnot be obser.ed,which indicated the low exp.ession level of these two NAD kinase homologs.

关键词： NAD kinase Sacchar.myces cer.visiae mitochondr.al function temp.r.tur. sensitivity subcellular.localization

Influence of Lactobacillus E1 on the Stor.ge Stability in Emulsion Immobilization

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Jour.al of Wuhan university of Technology(Mater.als Science) 2009年第1期24卷 75-80页

作者：孙欣陈西广 College of Life Science Ocean University of China

A coacer.ation method with double emulsion str.tegy （w/o/w） was used to p.ep.r. immobilized Lactobacillus E1. Diatomite was chosen as the car.ier.for.bacter.a. Sodium alginate, dextr.n and gelatin wer. used as p.otective solutes for.the p.eser.ation of Lactobacillus E1 and their.effects on the stor.ge viability dur.ng stor.ge wer. discussed. The influence of stor.ge temp.r.tur. on the stor.ge viability was also examined. The r.sults show that high bacter.a viable count over.109 cfu/g for.an extended shelf life of 37 d can be achieved with 2. sodium alginate, 5% dextr.n and 4% gelatin as p.otective solutes, at 10 ℃ of the stor.ge temp.r.tur.. This immobilized Lactobacillus E1 has p.tential use as functional food ingr.dient for.both human dair. food and animal feedstuff.

关键词： lactobacillus micr.encap.ulation stor.ge viability p.otective solute shelf life

p.anning Envir.nmental Imp.ct Assessment Or.enting Sustainable Develop.ent: Op.or.unities and Challenges

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Chinese Jour.al of p.p.lation,r.sour.es and Envir.nment 2005年第4期3卷 33-39页

作者： Sun Yanjun 1, Chen Xingeng 1, Bao Yun 2. p.ng Xiaochun 3 & Gao Changbo 1 1.school of Envir.nmental Science and engineering, Sun Yat-sen {2., Guangzhou 5102.5, china 2. r.sear.h Center.for.Coastal ocean Science and Technology, Sun Yat-sen {2., Guangzhou 5102.5, china 3. South china Institute of Envir.nmental Science, SEp., Guangzhou 510655, china

Str.tegic Envir.nmental Assessment is a fr.ntier.subject in the field of Envir.nmental Imp.ct Assessment. In the p.st two decades, esp.cially in r.cent year., much mor. imp.r.ance has been attached to Str.tegic Envir.nmental Assessment. The Envir.nmental Imp.ct Assessment Law of the p.r. china which was p.omulgated p.ovides a gr.at op.or.unity for.the develop.ent of p.anning Envir.nmental Imp.ct Assessment and br.ngs gr.at challenges for.the develop.ent of tr.ditional p.oject Envir.nmental Imp.ct Assessment and p.anning Envir.nmental Imp.ct Assessment at the same time. In or.er.to p.omote the imp.ementation of "The EIA Law", the inher.nt limitations of p.oject Envir.nmental Imp.ct Assessment must be identified sufficiently and the theor. r.sear.h and p.actice of p.anning Envir.nmental Imp.ct Assessment should be str.ngthened as well. Measur.s should be taken cur.ently to enfor.e the op.r.tion system. The author. wish to offer.a few r.fer.nces to the p.ogr.ss and imp.ementation of p.anning Envir.nmental Imp.ct Assessment in china.

关键词： p.anning envir.nmental imp.ct assessment str.tegic envir.nmental assessment sustainable develop.ent op.or.unities challenges.