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低分子肝素对经皮冠状动脉内介入治疗所致血液流变学异常的治疗作用

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中国生化药物杂志 2004年第6期25卷 361-362页

作者：方征李方胡燕燕田庆印济南铁路中心医院保健科山东济南250001 山东大学齐鲁医院心内科山东济南250012

目的评价低分子肝素 (LMWH)对经皮冠状动脉内介入治疗 (PCI)引起的血液流变学异常的治疗作用。方法选行PCI的冠心病患者 5 6例 ,随机分成A组 (2.9例 )和B组 (2.7例 ) ,均服用PCI围术期常规药物 ,A组于PCI术后即刻加用LMWH注射液 0 .4～ ... 详细信息

目的评价低分子肝素 (LMWH)对经皮冠状动脉内介入治疗 (PCI)引起的血液流变学异常的治疗作用。方法选行PCI的冠心病患者 5 6例 ,随机分成A组 (2.9例 )和B组 (2.7例 ) ,均服用PCI围术期常规药物 ,A组于PCI术后即刻加用LMWH注射液 0 .4～ 0 .6ml,2. /d ,皮下注射 ,连用 14d。检测两组患者PCI术前及术后 2.4h、7d、14d时的血液流变学指标。结果两组血液流变学指标术前无显著差异 (P >0 .0 5 ) ;术后 2.4h较术前显著增高 (A组P<0 .0 5 ,B组P <0 .0 1) ,B组增高更明显 (P <0 .0 5 ) ;A组术后 7d恢复至术前水平 ,B组术后 14d恢复至术前水平。结论LMWH可减轻PCI引起的血液流变学异常。

关键词：经皮冠状动脉内介入治疗低分子肝素血液流变学

Expert Consensus by qilu hospital of{2. on the diagnosis,management,and treatment of suspected COVID-19 cases(English version)

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Emergency and Critical Care Medicine 2021年第1期1卷 6-11页

作者： Yuguo Chen Expert Consensus Group of qilu hospital of{2. on Diagnosis and Treatment for COVID-19 Department of Emergency and Chest Pain Center Clinical Research Center for Emergency and Critical Care Medicine of Shandong ProvinceInstitute of Emergency and Critical Care Medicine of Shandong UniversityKey Laboratory of Emergency and Critical Care Medicine of Shandong ProvinceKey Laboratory of Cardiopulmonary-Cerebral Resuscitation Research of Shandong ProvinceQilu HospitalCheeloo College of MedicineShandong UniversityJinanShandongChina

We devised a protocol to establish a standardized method of screening,diagnosing,and managing suspected cases of coronavirus disease(COVID-19)and to enhance the management of COVID-19 suspected *** protocol that included diagnostic criteria,preventive measures,and control measures against COVID-19 was developed based on new evidence regarding the epidemiological and clinical characteristics of COVID-19.A consensus document was subsequently *** consensus focused on the clinical management of patients with suspected fever and reviewed the procedure for undergoing severe acute respiratory syndrome coronavirus 2.SARS-CoV-2.nucleic acid *** consensus will contribute to the ongoing efforts worldwide for the prevention and control of COVID-19.

关键词： COVID-19 Nucleic acid testing Suspected cases

Establishment and Application of a TaqMan Real-Time Quantitative Reverse Transcription-Polymerase Chain Reaction Assay for Rubella Virus RNA

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Acta Biochimica et Biophysica Sinica 2006年第10期38卷 731-736页

作者： Li-Hong ZHAO~1* Yu-Yan MA~2.Hong WANG~1 Shu-Ping ZHAO~3 Wei-Ming ZHAO~1 Hua LI~2.Lei-Yi WANG~2. 1 Department of Microbiology,School of Medicine,shandong university,{2. 250012,china 2.qilu hospital,shandong university,{2. 250012,china 3 Tai'an central hospital,Tai'an 2.1000,china 4 Department of Virology,School of Public Health,shandong university,{2. 250012,china Department of Microbiology School of Medicine Shandong UniversityJinan 250012 China qilu hospital Shandong UniversityJinan 250012 China Tai'an central hospital Tai'an 271000 China

The aim of this study was to establish and apply a real-time quantitative reverse transcription-polymerase chain reaction （RT-PCR） for rubella virus （RV） ***,the primer and TaqMan probeconcentrations,as well as reaction temperatures were optimized to establish an efficient real-time quantitativeRT-PCR assay for RV ***,an RV-specific PCR amplicon was made as an external standard toestimate the linearity,amplification efficiency,analytical sensitivity and reproducibility of the real timequantitative ***,the assay was applied to quantify RV RNA in clinical samples for rubella *** RV-specific PCR amplicon was prepared for evaluation of the assay at 503 bp,and its original concentrationwas 2.75×10～9 copies/μ*** real time quantitative assay was shown to have good linearity （R2.0.992.）,highamplification efficiency （E=1.91）,high sensitivity （2.5 copies/ml）,and high reproducibility （variation coefficientrange,from 1.2.% to 3.58%）.Compared with the gold standard,the specificity and sensitivity of the assayin clinical samples was 96.4% and 86.4%,***,the established quantitative RT-PCRmethod is a simple,rapid,less-labored,quantitative,highly specific and sensitive assay for RV RNA.

关键词： rubella virus PCR amplicon real-time fluorescence quantitative RT-PCR

Antitumor Effect of Antisense Ornithine Decarboxylase Adenovirus on Human Lung Cancer Cells

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Acta Biochimica et Biophysica Sinica 2006年第6期38卷 410-416页

作者： Hui TIAN~(1*),Lin LI~1,Xian-Xi LIU~2.and Yan ZHANG~2. ~1 Department of Thoracic Surgery,shandong university Qi Lu {2.,jinan 250012,china ~2.Experimental Center of Medical Molecular Biology,School of Medicine,shandong university,jinan 250012,china Department of Thoracic Surgery Shandong University Qi Lu HospitalJinan 250012 China Experimental Center of Medical Molecular Biology School of Medicine Shandong UniversityJinan 250012 China

Ornithine decarboxylase (ODC),the first enzyme of polyamine biosynthesis,was found toincrease in cancer cells,especially lung cancer *** chemotherapeutic agents aimed at decreasingODC gene expression showed inhibitory effects on cancer cells,ha this study,we examined the effects ofadenoviral transduced antisense ODC on lung cancer *** adenovirus carrying antisense ODC(rAd-ODC/Ex3as) was used to infect lung cancer cell line *** 3-(4,5-methylthiazol-2.yl)-2.5-diphenyl-tetrazolium bromide assay was used to analyze the effect on cell *** of ODC and concentrationof polyamines in cells were determined by Western blot analysis and high performance liquid *** deoxynucleotidyl transferase-mediated biotin-dUTP nick-end labeling was used to analyze *** expression of ODC in A-549 cells was reduced to 54%,and that of three polyamines wasalso decreased through the rAd-ODC/Ex3as ***,cell growth was substantially inhibitedand terminal deoxynucleotidyl transferase-mediated biotin-dUTP nick-end labeling showed that rAd-ODC/Ex3ascould lead to cell apoptosis,with apoptosis index of 46%.This study suggests that rAd-ODC/Ex3as has anantitumor effect on the human lung cancer cells.

关键词： polyamine putrescine biosynthesis antisense technology gene therapy A-549 lung cancer cell line

Effects of Raloxifene on Caveolin-1 mRNA and Protein Expressions in Vascular Smooth Muscle Cells

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 747-752页

作者： Fa-Lin YANG~(1#*) Hong HE~(2.) Xian-Xi LIU~1* Bing TU~1 Xian-Wei ZENG~3 Ji-Xin SU~1 Xin WANG~2.Qin HU~2. 1 Institute of Biochemistry and Molecular Biology,Medical College,shandong university,{2. 250012,china 2.Department of Cardiology,qilu hospital,shandong university,{2. 250012,china 3 Department of Clinical Medicine,Weifang Medical College,Weifang 2.0041,china Institute of Biochemistry and Molecular Biology Medical College Shandong UniversityJinan 250012 China Department of Cardiology Qilu Hospital Shandong UniversityJinan 250012 China Department of Clinical Medicine Weifang Medical CollegeWeifang 260041 China

Caveolin-1 is regulated by estrogen in vascular smooth muscle ***,a selectiveestrogen receptor modulator that possibly has cardioprotective properties without an increased risk of canceror other side effects of estrogen,may be used in women with risk of coronary artery ***,therelationship between raloxifene and caveolin-1 is still ***,this study was designed to seewhether raloxifene regulates caveolin-1 expression and if so,whether such regulation is mediated by *** aortic smooth muscle cells were cultured in the absence or presence of raloxifene (10-8to10-6M) for 12.or 2. *** mRNA and protein levels of caveolin-1 were increased significantly after 2. htreatment with *** increases were inhibited by estrogen receptor antagonist ICI 182.80 (10-5M).Results of this study suggest that raloxifene stimulates caveolin-1 transcription and translation throughestrogen receptor mediated mechanisms.

关键词： raloxifene caveolin-1 estrogen receptor vascular smooth muscle cell

Adenovirus-mediated Expression of both Antisense Ornithine Decarboxylase and S-adenosylmethionine Decarboxylase Inhibits Lung Cancer Cell Growth

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Acta Biochimica et Biophysica Sinica 2007年第6期39卷 423-430页

作者： Hui TIAN~(1*), Xianxi LIU~2. Bing ZHANG~2. Qifeng SUN~1, and Dongfeng SUN~1 1 Department of Thoracic Surgery, Qi Lu hospital, shandong university{2., china 2.Department of Medicine, Medical Molecular Biology Experimental Center, shandong university{2. china Department of Thoracic Surgery Qi Lu Hospital Shandong UniversityJinan 250012 China Department of Medicine Medical Molecular Biology Experimental Center Shandong UniversityJinan 250012 China

Polyamine biosynthesis is controlled primarily by ornithine decarboxylase （ODC） and Sadenosylmethionine decarboxylase （AdoMetDC）. Antisense sequences of ODC and AdoMetDC genes were cloned into an adenoviral vector （named Ad-ODC-AdoMetDCas）. To evaluate the effects of recombinant adenovirus Ad-ODC-AdoMetDCas that can simultaneously express both antisense ODC and AdoMetDC, the human lung cancer cell line A-549 was infected with Ad-ODC-AdoMetDCas or the control vector. Viable cell counting, determination of polyamine concentrations, cell cycle analysis, and Matrigel invasion assays were carried out to assess the properties of tumor growth and invasiveness. Our study showed that adenovirus-mediated antisense ODC and AdoMetDC expression inhibits tumor cell growth through blocking the polyamine synthesis pathway. Tumor cells were arrested at the G1phase after gene transfer and the invasiveness was reduced. It suggested that the recombinant adenovirus Ad-ODC-AdoMetDCas might be a new anticancer reagent in the treatment of lung cancers.

关键词： ornithine decarboxylase S-adenosylmethionine decarboxylase polyamine lung cancer genetherapy

Gene Expression of Ornithine Decarboxylase in Lung Cancers and Its Clinical Significance

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 639-645页

作者： Hui TIAN~1* Qing HUANG~1 Lm LI~1 Xian-Xi LIU~2.Yan ZHANG~2. 1 Department of Thoracic Surgery,shandong university Qi Lu {2.,jinan 250012,china 2.Department of Medicine,Medical Molecular Biology Experimental Center,shandong university,jinan 250012,china Department of Thoracic Surgery Shandong University Qi Lu HospitalJinan 250012 China Department of Medicine Medical Molecular Biology Experimental Center Shandong UniversityJinan 250012 China

Lung cancer is one of the most lethal cancers in china because of its high incidence and *** decarboxylase （ODC）,an important enzyme in polyamine biosynthesis,is increased incancer *** chemotherapeutic agents aimed at reducing ODC expression show inhibitory effects oncancer cell growth,so ODC can be useful in gene diagnosis and gene therapy of *** this study,weexamined the effect of antisense ODC on lung cancer cells.A-549 cells were infected with rAd-ODC/Ex3as,a recombinant adenovirus containing the cytomegalovirus promoter,green fluorescent protein gene and 12.bp antisense *** cell cycle was evaluated by flow cytometry.A nude mouse xenograft model wasused in the tumorigenicity *** transcription-polymerase chain reaction,Western blot and immuno-histochernistry were used to study the expressions of ODC on lung *** was found that the growth ofcells infected with rAd-ODC/Ex3as was substantially inhibited and cells were arrested at *** with rAd-ODC/Ex3as can suppress tumor formation in a nude mouse xenograft *** of ODC mRNA and ODC protein levels in lung cancer tissues was significantly higher than thatin normal tissues （P<0.05）,which correlated significantly with the stage of lung cancer （P<0.05）.This studysuggested that rAd-ODC/Ex3as has antitumor activity in human lung cancer *** ODC gene might playan important role in lung cancer and the overexpression of ODC might be related to the occurrence anddevelopment of lung cancer.

关键词： polyamine biosynthesis lung neoplasm ornithine decarboxylase A-549 cell line

The Effect of Anti-Human CD134 Monoclonal Antibody on Phytohemagglutinin-Induced mRNA Expression of Perforin in Peripheral Blood Mononuclear Cells

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Cellular & Molecular Immunology 2005年第6期2卷 467-471页

作者： Ming Li~1 Yuanchao Zhang~(1,2. ~1Department of Rheumatology and Immunology,shandong Provincial hospital,shandong university,jinan 2.002.,china ~2.epartment of Rheumatology and Immunology,shandong Provincial hospital,shandong university,jinan 2.002.,shandong,china. Department of Rheumatology and Immunology Shandong Provincial Hospital Shandong University Jinan 250021 China

CD134, a member of the tumor necrosis factor receptor superfamily, plays a crucial role in T cell survival. In this study, peripheral blood mononuclear cells （PBMCs） stimulated with phytohemagglutinin （PHA, 50 μg/ml） were treated by CD134 mAb （1μg/ml, 5 μg/ml, 10 μg/ml） for 6 h, 12.h, 2. h and 48 h. The level of perforin mRNA was measured by reverse transcription-polymerase chain reaction （RT-PCR） technique. Our data showed that the expression of perforin mRNA in PBMCs was down-regulated by CD134 mAb in a dose-dependent manner in range of 1 μg/ml to 5 μg/ml and dropped down to its minimum on 2. h （p 〈 0.05）. The level of perforin mRNA reached a plateau when the concentration of CD134 mAb exceeded 5 μg/ml. In conclusion, CD134 mAb can inhibit perforin expression, which may enhance the ability of T cells for survival. Cellular ＆ Molecular Immunology. 2.05;2.6）： 467-471.

关键词： CD134 perforin phytohemagglutinin

DFT study of the conductance of molecular wire:The effect of coupling geometry and intermolecular interaction on the transport properties

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Science china Chemistry 2006年第6期49卷 492-498页

作者： QI Yuanhua1,2. GUAN Daren1 & LIU Chengbu1 1. Institute of Theoretical Chemistry, shandong university, jinan 2.0100,{2. 2. School of Physics and Microelectronics, shandong university, jinan 2.0061,{2. Institute of Theoretical Chemistry Shandong University Jinan China School of Physics and Microelectronics Shandong University Jinan China

The density functional theory (DFT) combining with the non-equilibrium Green functions (NEGF) method is applied to the study of the electronic transport properties for a Di-thiol-benzene (DTB) molecule coupled to two Au(111) surfaces. The dependence of the transport properties on the bias, the coupling geometry of the molecule-electrode interface, and the intermolecular interaction are examined in detail. The results show that the existence of the hydrogen atom at the end of the DTB molecule would significantly decrease the transmission coefficients, and then the differential conductance (dI/dV). By changing the position of the DTB molecule located between two electrodes a maximum value of calculated current is observed. It is also found that the intermolecular interaction will strongly influence the transport properties of the system studied.

关键词： non-equilibrium Green functions method, transmission spectrum, the molecular projected selfconsistent Hamiltonian, projected density of state.