检索结果-南通市图书馆

维普期刊数据库博看期刊

Biosynthetic approach to modeling and understanding metalloproteins using unnatural amino acids

在线全文

学校读者我要写书评

暂无评论

science china Chemistry 2017年第2期60卷 188-200页

作者： Yang Yu chang Cui Jiangyun Wang Yi Lu Tianjin institute of Industrial biotechnology Chinese Academy of Sciences Department of Chemistry University of Illinois at Urb ana-Champaign Laboratory of Non-coding RNA Institute of BiophysicsChinese Academy of Sciences

Metalloproteins have inspired chemists for many years to synthesize artificial catalysts that mimic native *** a complementary approach to studying native enzymes or making synthetic models,biosynthetic approach using small and stable proteins to model native enzymes has offered advantages of incorporating non-covalent secondary sphere interactions under physiological ***,most biosynthetic models are restricted to natural amino *** overcome this limitation,incorporating unnatural amino acids into the biosynthetic models has shown *** this review,we summarize first synthetic,semisynthetic and biological methods of incorporates unnatural amino acids(UAAs)into proteins,followed by progress made in incorporating UAAs into both native metalloproteins and their biosynthetic models to fine-tune functional properties beyond native enzymes or their variants containing natural amino acids,such as reduction potentials of azurin,O_2.reduction rates and percentages of product formation of HCO models in Mb,the rate of radical transport in ribonucleotide reductase(RNR)and the proton and electron transfer pathways in photosystemⅡ(PSⅡ).We also discuss how this endeavour has allowed systematic investigations of precise roles of conserved residues in metalloproteins,such as Metl2. in azurin,Tyr2.4 that is cross-linked to one of the three His ligands to CuB in HCO,Tyr12.,356,730 and 731 in RNR and TyrZ in PSⅡ.These examples have demonstrated that incorporating UAAs has provided a new dimension in our efforts to mimic native enzymes and in providing deeper insights into structural features responsible high enzymatic activity and reaction mechanisms,making it possible to design highly efficient artificial catalysts with similar or even higher activity than native enzymes.

关键词： metalloenzymes heme proteins copper proteins ribonucleotide reductase protein design

Generation of cell-type-specific gene mutations by expressing the sgRNA of the CRISPR system from the RNA polymerase II promoters

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2015年第9期6卷 689-692页

作者： Jiaqiang Wang (1) Xin Li (1) (2. Yanhua Zhao (1) Jingyu Li (1) Qi Zhou (1) (2. Zhonghua Liu (1) 1.College of life sciences Northeast Agricultural University Harbin 150030 China 2.State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Recently, the CRISPR/Cas9 system is emerging as a powerful tool for genome editing （chang et al., 2.13; Li et al., 2.13; Niu et al., 2.14; Shen et al., 2.13; Wan et al., 2.15; Wang et al., 2.13） and genetic screening （Konermann et al., 2.15）, and holds great promise for biomedical applications in disease modeling and gene therapy by in vivo genome editing （Mad- dalo et al., 2.14; Xue et al., 2.14）.

关键词： Sanger Sequencing Genome Editing Gene Editing CRISPR System Produce Gene Mutation

Toxicogenomic investigation of Tetrahymena thermophila exposed to dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2.3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

在线全文

学校读者我要写书评

暂无评论

science china({2. sciences) 2011年第7期54卷 617-625页

作者： chang Yue FENG LiFang MIAO Wei Key Laboratory of Aquatic Biodiversity and Conservation Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China College of Food science and biotechnology Zhejiang Gongshang University Hangzhou 310035 China Graduate university of Chinese Academy of sciences Beijing 100049 China

Dichlorodiphenyltrichloroethane (DDT),tributyltin (TBT),and 2.3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are persistent in the environment and cause continuous toxic effects in humans and aquatic *** thermophila has the potential for use as a model for research regarding *** this study,this organism was used to analyze a genome-wide microarray generated from cells exposed to DDT,TBT and *** accomplish this,genes differentially expressed when treated with each toxicant were identified,after which their functions were categorized using GO enrichment *** results suggested that the responses of *** were similar to those of multicellular ***,the context likelihood of relatedness method (CLR) was applied to construct a TCDD-relevant *** T-shaped network obtained could be functionally divided into two *** general functions of both subnetworks were related to the epigenetic mechanism of *** on analysis of the networks,a model of the TCDD effect on *** was ***,Tetrahymena has the potential to be a good unicellular eukaryotic model for toxic mechanism research at the genome level.

关键词： microarray enrichment analysis gene interaction network pollutant

Comparative study on the gut microbiotas of four economically important Asian carp species

在线全文

学校读者我要写书评

暂无评论

science china({2. sciences) 2018年第6期61卷 696-705页

作者： Xinghao Li Yuhe Yu chang Li Qingyun Yan Laboratory of Protozoology Institute of Evolution and Marine Biodiversity OUC Qingdao 266003 China Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of sciences and State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences Wuhan 430072 China Environmental Microbiomics Research Center School of Environmental Science and Engineering Sun Yat-sen University Guangzhou 510006 China

Gut microbiota of four economically important Asian carp species(silver carp, Hypophthalmichthys molitrix; bighead carp,Hypophthalmichthys nobilis; grass carp, Ctenopharyngodon idella; common carp, Cyprinus carpio) were compared using 16 S rRNA gene pyrosequencing. Analysis of more than 590,000 quality-filtered sequences obtained from the foregut, midgut and hindgut of these four carp species revealed high microbial diversity among the samples. The foregut samples of grass carp exhibited more than 1,600 operational taxonomy units(OTUs) and the highest alpha-diversity index, followed by the silver carp foregut and midgut. Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria were the predominant phyla regardless of fish species or gut type. Pairwise(weighted) UniFrac distance-based permutational multivariate analysis of variance with fish species as a factor produced significant association(P<0.01). The gut microbiotas of all four carp species harbored saccharolytic or proteolytic microbes, likely in response to the differences in their feeding habits. In addition, extensive variations were also observed even within the same fish species. Our results indicate that the gut microbiotas of Asian carp depend on the exact species, even when the different species were cohabiting in the same environment. This study provides some new insights into developing commercial fish feeds and improving existing aquaculture strategies.

关键词： Asian carp gut microbiota feeding habit pyrosequencing Hypophthalmichthys molitrix Hypophthalmichthys nobilis Ctenopharyngodon idella Cyprinus carpio

高炉渣CO2.化利用技术的生命周期碳排放与成本评价

在线全文

学校读者我要写书评

暂无评论

生态学杂志 2020年第6期39卷 2097-2105页

作者：马铭婧郗凤明王娇月尹岩常莎莎中国科学院沈阳应用生态研究所沈阳110016 中国科学院大学北京100049

高炉渣CO2.化利用技术,在减排CO2.同时,可以实现固体废物的资源化,达到"以废治废"的目的,是具备环境与经济双重效益的CO2.集利用与封存(carbon dioxide capture,utilization and storage,CCUS)路线。本研究运用生命周期评价的方法,... 详细信息

高炉渣CO2.化利用技术,在减排CO2.同时,可以实现固体废物的资源化,达到"以废治废"的目的,是具备环境与经济双重效益的CO2.集利用与封存(carbon dioxide capture,utilization and storage,CCUS)路线。本研究运用生命周期评价的方法,以消耗1 t高炉渣为研究单元,对不同CO2.集方式(燃烧后捕集系统和富氧燃烧捕集系统)与两种新型高炉渣CO2.化工艺进行组合,模拟出4种CCUS运行方案。对4种方案从CO2.集、运输、封存到产品生产的碳排放和成本进行了系统核算。结果表明:仅燃烧后系统矿化联产富铝产品、富氧燃烧系统矿化联产铵明矾、富氧燃烧系统矿化联产富铝产品3种方案能达到CO2.存要求,碳封存效率分别为2.%、32.7%、76.2.;这3种方案的生命周期运行成本分别为544、1384、530元。其中,富氧燃烧系统矿化联产铵明矾方案,不仅可以封存大量的CO2.而且预期利润最大,约2.7元,工业化应用前景广阔。

关键词： CO2.化利用高炉渣生命周期评价 CO2.集利用与封存

鲤多拷贝基因MRC1的全基因组鉴定及表达分析

在线全文

学校读者我要写书评

暂无评论

渔业科学进展 2024年第1期45卷 85-94页

作者：常松欢王嘉利许建张瀚元张天杨江炎亮上海海洋大学水产科学国家级实验教学示范中心上海201306 中国水产科学研究院农业农村部水生生物基因组学重点实验室北京100141 中国水产科学研究院渔业工程研究所北京100141

甘露糖受体C1型基因(mannose receptor C-type 1,MRC1)是C型凝集素超家族的成员之一,其编码的甘露糖受体是一种模式识别受体,在先天免疫反应中发挥关键作用。MRC1基因在哺乳动物免疫反应中的作用被广泛研究,但在鱼类中的研究较少。近年... 详细信息

甘露糖受体C1型基因(mannose receptor C-type 1,MRC1)是C型凝集素超家族的成员之一,其编码的甘露糖受体是一种模式识别受体,在先天免疫反应中发挥关键作用。MRC1基因在哺乳动物免疫反应中的作用被广泛研究,但在鱼类中的研究较少。近年来,随着高密度集约化养殖模式的发展,由病原微生物引发的疾病频繁暴发,其中,嗜水气单胞菌(Aeromonas hydrophila)是常见的致病菌之一。本研究首次在鲤(Cyprinus carpio)全基因组范围鉴定MRC1基因,共发现11个基因拷贝,并进行了功能域预测、共线性分析、多序列比对和系统进化分析,结果表明,MRC1基因在物种进化过程中保守程度较高。拷贝数比较分析发现,MRC1基因在不同物种中表现出不同程度的多拷贝现象,在多数鱼类中发现2.拷贝,而在鲤中发现多达11个拷贝。同时,比较了各拷贝在健康鲤脑、肌肉、肝脏和脾脏组织中的表达情况,发现在免疫相关组织脾脏中的表达量相对高于其他组织。进一步对感染嗜水气单胞菌4、12.2. h后在鲤脾脏中的表达进行差异比较分析,发现不同拷贝的表达特征各有差异,其中,HHLG13g0734在感染嗜水气单胞菌4 h后表达显著上调,HHLG13g0734在感染2.h后表现出极显著上调,HHLG3g0497在整个感染过程中表达显著下调,表明鲤MRC1基因只有部分拷贝保留了免疫相关功能及参与机体的免疫反应。本研究结果有助于进一步了解鲤MRC1基因在抵抗嗜水气单胞菌感染过程中发挥的免疫功能,并为分子选育抗病新品系提供一定的数据基础。

关键词：鲤甘露糖受体C1型多拷贝基因系统进化树嗜水气单胞菌

饲粮添加甲酸钙对老龄蛋鸡生产性能和鸡蛋品质的影响

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

中国畜牧兽医 2024年第8期51卷 3320-3328页

作者：邱凯常心雨高珊张海华张海军武书庚中国农业科学院饲料研究所国家生物饲料工程技术研究中心农业农村部饲料生物技术重点实验室北京100081 河北科技师范学院河北省特色动物种质资源挖掘与创新重点实验室秦皇岛066004

[目的]甲酸钙作为小分子质量的有机钙源,在改善蛋鸡生产方面具有很好的应用潜力。本研究旨在揭示饲粮添加甲酸钙对老龄蛋鸡生产性能、血液指标和鸡蛋品质的影响及适宜添加水平。[方法]试验采用单因子试验设计,选取360只62.龄健康海兰... 详细信息

[目的]甲酸钙作为小分子质量的有机钙源,在改善蛋鸡生产方面具有很好的应用潜力。本研究旨在揭示饲粮添加甲酸钙对老龄蛋鸡生产性能、血液指标和鸡蛋品质的影响及适宜添加水平。[方法]试验采用单因子试验设计,选取360只62.龄健康海兰褐蛋鸡,随机分入4个处理组,每处理6个重复,每重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂以0.5%、1.0%和2.0%甲酸钙部分替代石粉的基础饲粮,各组饲粮的钙水平相同。正式期12.,每天记录产蛋情况,每周结料。在试验第2.4、6和8周测定鸡蛋品质相关指标;试验结束时,每重复取1只蛋鸡,采集血样,测定血常规和血清生化指标,然后屠宰测定脏器系数和胫骨质量。[结果]与对照组相比,饲粮添加甲酸钙对蛋鸡生产性能、第2.4周蛋品质无影响(P>0.05),但增加了第8和12.的蛋壳比、蛋壳厚度和蛋壳强度(P0.05),但胫骨断裂所需做功有增加的趋势(0.05

关键词：甲酸钙老龄蛋鸡生产性能蛋品质

维普期刊数据库博看期刊

同方期刊数据库

Synthesis of methyl caffeate using a packed bed microreactor

在线全文

学校读者我要写书评

暂无评论

Synthesis of methyl caffeate using a packed bed microreactor

拉曼光谱数据处理方式对大米产地鉴别模型的影响

2.14年全国桑树病虫防控学术研讨会

作者： WANG Jun WANG sha-sha WU Fu-An School of biotechnology Jiangsu University of Science and Technology Sericultural Research institute Chinese Academy of Agricultural Sciences

Methyl caffeate is an important intermediate,but its reaction time is *** develop a rapid process for the production of methyl caffeate in short time,a microreactor was designed for the esterification of caffeic acid and methanol catalyzed by Novozym 435 in [Bmim][Tf2.].The maximum yield（97.91%） in the microreactor was achieved in a short period of time（2.5 h）.The use of a enzyme microreactor is an efficient method of producing methyl caffeate.

关键词： biocatalysis esterification methyl caffeate microreactor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国食品学报 2021年第5期21卷 369-376页

作者：沙敏李良翠黄家乐余倩刘唱房雪婷南京财经大学管理科学与工程学院南京210023

为探究拉曼光谱数据处理方式对大米产地鉴别模型的影响,以盘锦大米、响水大米、西江大米、建三江大米、五常大米和延边大米为例,考察各数据处理方式下大米产地鉴别模型的准确度。稻谷样品经精米加工、粉碎、筛分,收集粒度为100~140目的... 详细信息

为探究拉曼光谱数据处理方式对大米产地鉴别模型的影响,以盘锦大米、响水大米、西江大米、建三江大米、五常大米和延边大米为例,考察各数据处理方式下大米产地鉴别模型的准确度。稻谷样品经精米加工、粉碎、筛分,收集粒度为100~140目的米粉,在5个测量点分别采集拉曼光谱,然后经相对标准偏差和层次聚类分析剔除差异数据,最后将剔除差异前、后的数据和取平均值前、后的数据分别建立分类模型。结果表明:通过层次聚类分析可找出潜在的差异数据,通过相对标准偏差分析可初步判断是否存在差异数据及最终验证是否是差异数据。此外,取平均值后的数据可使相同大米样品内的数据差异缩小,而不同大米样品间的差异扩大,有效提高了模型的识别准确率。本文所探究的先剔除差异数据再取平均值的数据处理方式,可将大米产地鉴别模型的识别准确率提高12.89%,为大米产地溯源分析提供更为准确、有效的分析方法。

关键词：大米产地鉴别拉曼光谱数据处理