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Bio-regeneration of p-complexation desulfurization adsorbents

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Science china Chemistry 2005年第6期48卷 538-544页

作者： LI Wangliang1,2. XING Jianmin1, XIONG Xiaochao1,2. SHAN Guobin1,2.& LIU Huizhou1 1. Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese academy of sciences, {2. 100080, china 2. graduate School of the Chinese academy of sciences,{2. 100039, china Laboratory of Separation Science and Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing China graduate School of the Chinese academy of {2. Beijing China

The coupling of adsorption desulfurization and biodesulfurization is a new approach to produce clean fuels. Sulfur compounds are firstly adsorbed on adsorbents, and then the ad-sorbents are regenerated by microbial conversion. p-Complexation adsorbent, Cu(I)-Y, was ob-tained by ion exchanging Y-type zeolite with Cu2. and then by auto-reduction in helium at 450℃ for 3 h. Dibenzothiophene (DBT) was used as a model compound. The effects of cell concentra-tion, volume of oil phase, the ratio of aqueous phase to adsorbent on DBT desorption by a bac-terium were studied. The amounts of DBT desorbed and 2.HBP produced can be apparently increased with addition of n-octane. BDS activity can be improved by increasing cell concentra-tion and the ratio of water-to-adsorbent. 89% of DBT desorbed from the adsorbents can be converted to 2.HBP within 6 h and almost 100% within 2. h, when the volume ratio of oil-to-water was 1/5 mL/mL, the cell concentration was 60 g·L?1, and the ratio of adsorbent-to-oil was 0.03 g·mL?1. The amount of 2.HBP produced was strongly dependent on the volume ratio of oil-to- water, cell concentration and amount of adsorbent. Adsorption capacity of the regenerated ad-sorbent is 95% that of the fresh one after being desorbed with Pseudomonas delafieldii R-8, washed with n-octane, dried at 100℃ for 2. h and auto-reduced in He.

关键词： adsorption desulfurization p-complexation adsorbents bio-regeneration Pseudomonas delafieldii desorption.

The deep thermal characteristic of continental margin of the northern South china Sea

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Chinese Science Bulletin 2000年第18期45卷 1717-1722页

作者： ZHANG Jian & WANG Jiyang1. graduate School, university of Science and Technology of{2., Chinese academy of sciences, beijing 100039,{2. 2. Institute of Geology, Chinese academy of sciences, beijing 10002.,{2. graduate School University of Science and Technology of China Chinese Academy of Sciences Beijing China Institute of Geology Chinese Academy of Sciences Beijing China

Heat flow plays an important role in the study ot thermal structure and thermal evolutionof continental margin of the northern South china Sea. The analysis of heat flow value shows that margin heat flow in the northern South china Sea is relatively high setting, but the percentage of crustal heat flow is lower than 35% in terrestrial heat flow. The terrestrial heat flow exhibited a current of rise from the Northern Continental Margin to the Southern Central Basin. However, the proportion of crustal heat flow in terrestrial heat flow slowly dropped down in the same direction. It is suggested that the main factor causing high heat flow setting is the moving up of hot material from asthenosphere.

关键词： margin of northern South china Sea terrestrial heat flow thermal structures.

Cloning and characterization of interferon stimulated genes Viperin and ISG15,and their promoters from snakehead Channa argus

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Progress in Natural Science:Materials International 2007年第12期17卷 1425-1435页

作者： Jia Weizhang1,2. Zhou Xiuxia1,2. Huang Rong1,2.and Guo Qionglin1(1. Institute of Hydrobiololgy, Chinese academy of sciences, Wuhan 430072.{2. 2. graduate university of Chinese academy of sciences, beijing 100039,{2.) Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China graduate university Chinese Academy of Sciences Beijing 100039 China

By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352.amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and γ-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2.recognition sites. GAS is responsible for the γ-IFN mediated transcription. One conserved site for NF-κB was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-κB in accordance with the consensus sequence (GGGRNNYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5′ untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I∶C.

关键词： interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus).

Molecular cloning of growth hormone receptor (GHR) from common carp(Cyprinus carpio L.)and identification of its two forms of mRNA transcripts

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Progress in Natural Science:Materials International 2006年第11期16卷 1156-1163页

作者： SUN Xiaofeng~ 1,2., GUO Qionglin~1**, HU Wei~1, WANG Yaping~1 and ZHU Zuoyan~1(1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese academy of sciences, Wuhan 430072.{2. 2. graduate School of Chinese academy of sciences, beijing 100039,{2.) State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China graduate School of Chinese academy of {2. Beijing 100039 China

The cDNA of growth hormone receptor (GHR) was cloned from the liver of 2.year common carp (Cyprinus carpio L.) by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE). Its open reading frame (ORF) of 1806 nucleotides is translated into a putative peptide of 602.amino acids, including an extracellular ligand-binding domain of 2.4 amino acids (aa), a single transmembrane domain of 2. aa and an intracellular signal-transduction domain of 334 aa. Sequence analysis indicated that common carp GHR is highly homologous to goldfish (Carassius auratus) GHR at both gene and protein levels. Using a pair of gene-specific primers, a GHR fragment was amplified from the cDNA of 2.year common carp, a 2.4 bp product was identified in liver and a 32. bp product in other tissues. The sequencing of the products and the partial genomic DNA indicated that the difference in product size was the result of a 97 bp intron that alternatively spliced. In addition, the 32. bp fragment could be amplified from all the tissues of 4-month common carp including liver, demonstrating the occurrence of the alternative splicing of this intron during the development of common carp. Moreover, a semi-quantitative RT-PCR was performed to analyze the expression level of GHR in tissues of 2.year common carp and 4-month common carp. The result revealed that in the tissues of gill, thymus and brain, the expression level of GHR in 2.year common carp was significantly lower than that of 4-month common carp.

关键词： growth hormone receptor (GHR) molecular cloning transcript expression analysis common carp.

Gasdynamic characteristics of toroidal shock and detonation wave converging

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Science china(Physics,Mechanics & Astronomy) 2005年第6期48卷 739-749页

作者： TENG Honghui1,2.& JIANG Zonglin2.1. Key Laboratory of High Temperature Gas Dynamics, Institute of Mechanics, Chinese academy of Sci- ences, beijing 100080,{2. 2. graduate university of Chinese academy of sciences, beijing 100039,{2. Key Laboratory of High Temperature Gas Dynamics Institute of Mechanics Chinese Academy of Sciences Beijing China graduate university of Chinese {2. of sciences Beijing China

The modified CCW relation is applied to analyzing the shock, detonation wave converging and the role of chemical reactions in the process. Results indicate that the shock wave is strengthened faster than the detonation wave in the converging at the same initial Mach number. Euler equations implemented with a detailed chemical reaction model are solved to simulate toroidal shock and detonation wave converging. Gasdynamic characteristics of the converging are investigated, including wave interaction patterns, observable discrepancies and physical phenomena behind them. By comparing wave diffractions, converging processes and pressure evolutions in the focusing area, the different effects of chemical reactions on diffracting and converging processes are discussed and the analytic conclusion is demonstrated through the observation of numerical simulations.

关键词： converging, CCW relation, shock wave, detonation wave.

Nonlinear interactions between gravity waves and background winds

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Progress in Natural Science:Materials International 2007年第6期17卷 639-644页

作者： Liu Xiao1,2.and Xu Jiyao1(1. State Key Laboratory for Space Weather, Center for Space Science and Applied Research, Chinese academy of sciences, {2. 100080, china 2. graduate university of Chinese academy of sciences, beijing 100039, china) State Key Laboratory for Space Weather Chinese Academy of Sciences Beijing 100080 China Center for Space Science and Applied Research Chinese Academy of Sciences Beijing 100080 China graduate university Chinese Academy of Sciences Beijing 100039 China

Using the nonlinear propagating gravity waves (GW) model in the two-dimensional compressible atmosphere and the linear GW theory, the process of GW propagation in different background winds, e.g. the direction of the background wind is opposite to (dead wind) or the same as (tail wind) the direction of the horizontal phase velocity of GW, is studied. The results show that the dead wind prolongs the vertical wavelength and accelerates GW propagation. Therefore, GW propagates up to a higher height becomes instable in a short time and eventually induces an inverse jet flow. Then, the vertical wavelength is becoming short due to the nonlinear interactions between GW and the inverse jet flow. The vertical wavelength and group velocity decrease after GW propagates into the tail wind. The initial instable time is delayed. Although most of GW is trapped in the instable region, some of GW propagates above the instable region. Compared with GW propagation in the tail wind, the nonlinear interactions between GW and the dead wind are also strong. In contrast, the linear GW theory predicts that GW can propagate freely in the dead wind. The vertical wavelength simulated by the nonlinear numerical model is different from that predicted by the linear theory greatly after GW propagates into the dead wind.

关键词： gravity waves background winds vertical group velocity vertical wavelength nonlinear interactions.

Discovery of immune related factors in Fenneropenaeus chinensis by annotation of ESTs

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Progress in Natural Science:Materials International 2004年第1期14卷 48-55页

作者： SHEN Yaoqing 1,2. , XIANG Jianhai 1*, WANG Bing 1,2. , LI Fuhua 1 and TONG Wei 3(1. Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese academy of sciences, Qingdao 2.6071,{2. 2. graduate School of the Chinese academy of sciences, beijing 100039, china 3. Genomics & Bioinformatics Institute, Chinese academy of sciences, beijing 101300, china) Chinese Acad Sci Inst Oceanol Expt Marine Biol Lab Qingdao 266071 Peoples R China Chinese Acad Sci Grad Sch Beijing 100039 Peoples R China Chinese Acad Sci Geonom & Bioinformat Inst Beijing 101300 Peoples R China

A total of 10446 expressed sequence tags (ESTs) are obtained by a large scale sequencing of a cDNA library from cephalothorax of adult Fenneropenaeus chinensis . An EST analysis platform was built up based on local computers and bioinformatic techniques were used to annotate these ESTs in order to promptly find possible functional genes, especially for immune related factors. About 4% of the ESTs show similarity to the coding sequences of such factors, including lectin, serine protease, serpin, lysozyme, etc. These ESTs provide a partial profile of the immune system in F. chinensis and useful information for further study on these genes.

关键词： Fenneropenaeus chinensis bioinformatics ESTs immune factors

Parentage determination of an isolated Yangtze finless porpoise population Neophocaena phocaenoides asiaeorientalis in the Yangtze Tian-e-Zhou Baiji National Natural Reserve based on molecular data

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Progress in Natural Science:Materials International 2005年第2期15卷 54-61页

作者： XIA Junhong 1,2. ZHENG Jinsong 1,2. XU Limei 1,2.and WANG Ding1*(1. Institute of Hydrobiology, Chinese academy of sciences, Wuhan 430072.{2. 2. graduate School of the Chinese academy of sciences, beijing 100039,{2.) Inst. of Hydrobiology Chinese Acad. of Sciences Wuhan 430072 China graduate Sch. Chinese Acad. of Sciences Beijing 100039 China

Reproductive behaviors are poorly known for the Yangtze finless porpoise Neophocaena phocaenoides asiaeorientalis. In this study, the parentage of an isolated Yangtze finless porpoise population inhabiting the Yangtze Tian-e-Zhou Baiji National Natural Reserve is determined by analysis of microsatellite loci and mitochondrial DNA (mtDNA) control region sequences, and the porpoise's reproductive behaviors are studied. Overall 4 full parentage assignments and additional 3 single parentage assignments were determined for the population of 2. individuals. The analysis shows that their estimated reproductive cycle is shorter than that reported previously and there probably exists an overlapping between gestation and lactation period. The study also shows that the female does not show fidelity to a particular male for breeding and vice versa, the oldest males did not monopolize mating and the dominance rank could not be so strict for the porpoise society. Moreover, the porpoise's mating pattern and relatedness among candidate parents are discussed here. These results provide important information for making guidelines of management and conservation for this protected population.

关键词： Yangtze finless porpoise isolated population reproductive behavior parentage

Wet-chemical synthesis and characteristics of Au nanoshell

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Science china Chemistry 2005年第5期48卷 431-435页

作者： LIU Zhongxin1,2. SONG Hongwei1, YU Lixin1,2. YANG Linmei1,2.& PAN Guohui1,2.1. Key Laboratory of Excited State Physics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese academy of sciences, Changchun 130033,{2. 2. graduate School of the Chinese academy of sciences, beijing 100039,{2. Key Laboratory of Excited State Physics Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun China graduate School of the Chinese academy of {2. Beijing China

Gold nanoshells were prepared by an easy wet-chemical method, with Ag nanoparticles used as the templates. Transmission electron microscopy (TEM) indicated that the shells were sphere in shape, the size was homogenous, being about 2. nm, and no hard ag-glomerates were observed. The plasma resonance absorption of gold was tuned from visible to near-infrared (NIR) with the increased volume of HAuCl4. The temperature grads (?T) of the gold nanoshell hydrosol under the exposure of an 808-nm optical fiber laser with different power den-sities were measured. The highest ?T was 30℃ (5 W/cm2. irradiation area was 2.cm2.. This kind of gold nanoshell hydrosol is a promising material to be used in biomedicine such as photo-thermal cancer therapy, and its special photothermal convert property will photothermally trigger drug release.

关键词： wet-chemical synthesis, Au, nanoshell, NIR absorption, photothermal conversion.