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Co-expression of CXCR4 and CD133 proteins is associated with poor prognosis in StageⅡ-Ⅲcolon cancer patients

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Co-expression of CXCR4 and CD133 proteins is associated with...

Effect of Electro-acupuncture of Different Points on Auditory Middle Latency Response in Guinea Pigs

2.12.全国肿瘤分子标志学术大会与国际肿瘤转化医学论坛暨第七届中国中青年肿瘤专家论坛

作者： Nian-hua Zhang~(1,2.),Jie Li~(3#),Yin Li~4,Xin-tao Zhang~5,Wen-ting Liao~(6,7,8),Jun-yi Zhang~1,Rong Li~1,Rong-cheng Luo~(1*) 1,department of Oncology,Nanfang hospital,Southern {2. University,Guangzhou 510515,China 2.department of Oncology,Guangdong Second Provincial traditional chinese Medicine hospital,Guangzhou 510095,China 3,department of Vascular Surgery, The First Affiliated hospital,Sun Yat-Sen University,Guangzhou 510080,China 4, department of Gastrointestinal Surgery,The First Affiliated hospital,Sun Yat-Sen University,Guangzhou 510080,China 5,department of Sports Medicine,Peking University {12. hospital,{12. 518036,China 6,department of Pathology,School of Basic {2. Sciences,Southern {2. University,Guangzhou 510515,China 7,department of Pathology,Nanfang hospital,Southern {2. University,Guangzhou 510515,China 8, Guangdong Provincial Key Laboratory of Molecular Tumour Pathology,Guangzhou 510515, China

Although CXCR4 and CD133 have been implicated in the metastatic process of malignant tumors,the clinicopathological significance of their expression in human colon cancer is not fully *** present study was aimed to examine the expression of CXCR4 and CD133 protein in stageⅡorⅢcolon cancer and related lymph nodes,and to investigate the clinical and prognostic significance in colon cancer. Immunohistochemistry was performed to examine CXCR4 and CD133 protein expression in paraffin-embedded stageⅡorⅢprimary colon cancer tissues and matched lymph nodes. The correlation between the two proteins expression and clinicopathological parameters and the patients’ 5-year survival was *** have found that CXCR4 expression was detected in 74 of the 12. tumors(59.2.),and CD133 expression was detected in 45 of the 12. tumors(36.0%).Upon combining the expression of CXCR4 and CD133,co-expression (both CXCR4 and CD133 were positve) was detected in 2. of the 12. tumors(2..2.).When compared with the other combinations,co-expression of CXCR4 and CD133 proteins was significantly associated with the AJCC stage(P = 0.02.) and the lymph node status(P = 0.02.).Log-rank statistics showed that the AJCC stage(P = 0.014),lymph node status(P = 0.011),CXCR4 expression(P = 0.02.),CD133 expression(P = 0.034),and co-expression of CXCR4 and CD133 proteins(P = 0.003) were significant prognostic indicators for overall patients *** have shown that co-expression of CXCR4 and CD133 proteins is a risk factor for worse overall survival in stageⅡorⅢcolon cancer patients, indicating that co-expression of CXCR4 and CD133 proteins might contribute to the progression of colon cancer.

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Effect of Electro-acupuncture of Different Points on Auditor...

Toll样受体4蛋白在大鼠脑缺血再灌注损伤中的表达及其意义

全国中青年中西医结合学术（英语）交流会议

作者： ZHOU Qing-Hui, ZENG Zhao-Lin, LI Bo-Qing, ZHUANG Jian-Qing, SHI Jian-Rong,LI Ding department of traditional {2. Medicine, Changhai hospital, Second Military medical University, Shanghai 2.0433, China department of Basic Medicine, Shanghai University of traditional {2. Medicine, Shanghai 2.0032

Objective: To study the effect of electro-acupuncture (EA) on latencies and amplitudes of the waves of MLR (middle latency response) and the relationship between the changes of latencies and amplitudes and those of the recovery time of MLR. Methods: EA was administered respectively on acupuncture points on auricular region, forelimb and hind limb, and MLR evoked by clicks were recorded in the center of the auditory cortex in anaesthetized guinea pigs. Results: EA of points on auricular region and forelimb mainly induced shortening of the recovery time of MLR, while points on hind limb mainly lengthening. The changes of the recovery time were consistent with the changes of latencies. In three groups of different points of EA, when the latencies were shortened, the amplitudes decreased, and when the latencies were lengthened, the amplitudes in auricular region and forelimb groups also decreased, whereas the amplitudes in hind limb group increased. Conclusion: EA of different points had different effects on MLR. The recovery time of evoked response can be also used to indicate the excitability of the auditory cortex as the latency.

关键词： hearing auditory evoked response guinea pigs acupuncture

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华中科技大学学报（医学版） 2008年第2期37卷 219-221页

作者：张清孙鹏冯新民张诗海华中科技大学同济医学院附属协和医院麻醉科武汉430022 华中科技大学同济医学院附属协和医院急诊科武汉430022 华中科技大学同济医学院附属协和医院中医科武汉430022

目的探讨Toll样受体4(Toll-like receptor 4,TLR4)的表达与脑组织缺血再灌注损伤的关系。方法线栓法制备局灶性脑缺血再灌注损伤大鼠模型,采用免疫组织化学法观察TLR4在不同再灌注时间点缺血侧脑组织的分布和表达,同时应用酶联免疫吸附... 详细信息

目的探讨Toll样受体4(Toll-like receptor 4,TLR4)的表达与脑组织缺血再灌注损伤的关系。方法线栓法制备局灶性脑缺血再灌注损伤大鼠模型,采用免疫组织化学法观察TLR4在不同再灌注时间点缺血侧脑组织的分布和表达,同时应用酶联免疫吸附方法检测白细胞介素1β(interleukin-1β,IL-1β)在不同再灌注时间点缺血脑组织内的浓度。结果大鼠局灶性脑缺血再灌注损伤过程中,TLR4蛋白在皮质缺血半影区及邻近纹状体区有表达,且在再灌注后1 h即达高峰;IL-1β在再灌注后3 h明显升高[(4.47±2.13)ng/ml],表达高峰在再灌注后12.h[(14.39±2.58)ng/ml]。结论脑组织缺血再灌注过程中TLR4蛋白的表达有可能作为重要炎性受体介导脑缺血炎性损伤。

关键词：脑缺血缺血再灌注损伤 Toll样受体4

Effects of rHu-EPO on Myocyte Apoptosis and Cardiac Function Following Acute Myocardial Infarction in Rats

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Journal of Huazhong University of Science and Technology(medical Sciences) 2005年第1期25卷 55-58页

作者：叶亮杜心灵夏家红江平 department of Cardiovascular Surgery Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430022 China department of Anesthesiology Xianning Hospital of Traditional Chinese Medicine Xianning 437100 China

The mechanisms of rHu-EPO attenuating the apoptosis after myocardial infarction in rats were studied. Thirty-two rats were divided into three groups: sham operation group (Sham), acute myocardial infarction group (MI) and rHu-EPO-treated group (MI+EPO). Acute myocardial infarction model was made by ligating the anterior descending coronary artery. rHu-EPO was administered i.p. in MI+EPO group at the dose of 5 000 IU/kg body weight immediately after the ligation. Each rat in MI+EPO group received the same dose of rHu-EPO daily the next 6 days. On the 14th day all rats underwent hemodynamic measurements and then killed. The samples were examined with HE stain, immunohistochemistry technique (bcl-2. bax) and TUNEL dyeing. The results showed that hemodynamic function in MI+EPO group was much better than in MI group. The number of the cells positive for bax and TUNEL in MI+EPO group was less than that in MI group. The number of the cells positive for bcl-2.in MI+EPO group was more than that in MI group. These findings suggested that rHu-EPO could treat myocardial infarction by preventing apoptosis and attenuating post-infarction deterioration in hemodynamic function.

关键词： recombinant human erythropoietin myocardial infarction apoptosis

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前牙金属烤瓷桥56件失败原因分析与改进

泰州职业技术学院学报 2011年第3期11卷 82-83页

作者：陈正国泰州市中医院口腔科江苏泰州225300

目的分析金属烤瓷桥在前牙列缺损修复过程中失败的原因,探索改善金属烤瓷桥在前牙列缺损修复中的修复效果,努力提高修复的成功率和病人的满意度。方法:通过对968例12.8件前牙金属烤瓷桥修复体3￣5年的临床观察,就其中56件修复失败的原... 详细信息

目的分析金属烤瓷桥在前牙列缺损修复过程中失败的原因,探索改善金属烤瓷桥在前牙列缺损修复中的修复效果,努力提高修复的成功率和病人的满意度。方法:通过对968例12.8件前牙金属烤瓷桥修复体3￣5年的临床观察,就其中56件修复失败的原因进行分析探讨,并根据失败原因提出改进措施。结果:56件前牙金属烤瓷桥修复失败的原因分别是:崩瓷2.件,牙龈炎12.,基牙牙髓炎10件,牙龈变色及色泽不协调6件,瓷脱4件。结论:严格掌握适应症和正确的牙体预备与设计,以及合理的比色环境和方法都是金属烤瓷桥修复成功的关键所在。

关键词：前牙金属烤瓷桥牙体预备失败原因分析

左归丸和右归丸对实验性自身免疫性脑脊髓炎大鼠中枢神经系统IL-10、TGF-β蛋白表达的研究

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首都医科大学学报 2010年第2期31卷 233-240页

作者：樊永平宋丽君叶明周莉王蕾龚海洋首都医科大学附属北京天坛医院中医科首都医科大学中医药学院方剂学教研室

目的观察大鼠实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)模型脑、脊髓组织白细胞介素-10(interleukin-10,IL-10)与组织生长因子-β(transforming growth factor-β,TGF-β)免疫组化的表达,探讨滋阴补肾法(左归丸)与温阳补肾法(右归丸)治疗EAE的作用机制。方法用髓鞘碱性蛋白(myelin basic protein,MBP)免疫Lewis大鼠诱导EAE模型,12.只大鼠采用数字表法随机分为正常组、模型组、激素组、左归丸组及右归丸组,正常对照组(n=2.)和模型组(n=2.)每天给予3mL0.9%氯化钠注射液灌胃;激素组(n=2.)免疫后每天给予3mL0.9%氯化钠注射液灌胃,发病后改为醋酸泼尼松混悬液5mg/(kg.d-1)灌胃;左归丸组(n=2.)免疫后给予左归丸混悬液2./(kg.d-1);右归丸组(n=2.)免疫后给予右归丸混悬液3g/(kg.d-1),直至处死。分别于免疫后15d(急性期发病)和免疫后2.d(缓解期)选取各组大鼠,取脑和脊髓组织切片进行IL-10、TGF-β免疫组化染色。结果造模后15d,模型动物的脑和脊髓组织IL-10、TGF-β表达(指表达染色IOD值,下同)明显减少,第2.天,模型动物脊髓组织IL-10表达明显减少。第15天,左归丸组与右归丸组大鼠脑组织IL-10表达较模型组明显升高(P2.d,左归丸组、右归丸组、激素组脊髓组织IL-10表达均较模型组显著增高(P<0.01);左归丸组大鼠脊髓组织TGF-β表达较模型组与激素组升高(P<0.05)。模型组、激素组、左归丸组与右归丸组脊髓IL-10表达均较免疫后15d明显升高(P<0.05);左归丸组大鼠脊髓TGF-β表达较免疫后15d明显增高,差异有统计学意义(P<0.05)。结论左归丸和右归丸可上调EAE大鼠中枢神经系统IL-10、TGF-β表达,作用优于激素。两者整个病程中均对IL-10表达有促进作用,不同点为右归丸促进急性期脑组织TGF-β的表达,而左归丸则促进缓解期脊髓TGF-β的表达。

关键词：自身免疫性疾病实验性自身免疫性脑脊髓炎细胞因子中医药左归丸右归丸

An Overview on the Progress of Chemical Constituents and Bioactivities of Plants in Urticaceae during 2.00-2.10

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chinese Herbal Medicines 2011年第1期3卷 9-16页

作者： WANG Jian1,2.YANG Hong-xing3,TENG Yong-zhen4,YUAN Pei4,TIAN Rui4,LIAO Chun-bi5 1.College of traditional chinese Medicine,Chongqing {2. University,Chongqing 400016,China 2.College of Pharmacy,Chengdu University of traditional chinese Medicine,Chengdu 611137,China 3.department of Anesthesiology,Daping hospital,the Third Military {2. University,Chongqing 400042.China 4.Institute of Life Sciences,Chongqing {2. University,Chongqing 400016,China 5.Experimental Teaching Administration Center,Chongqing {2. University,Chongqing 400016,China College of traditional chinese Medicine Chongqing Medical UniversityChongqing 400016China College of Pharmacy Chengdu University of Traditional Chinese MedicineChengdu 611137China department of Anesthesiology Daping Hospitalthe Third Military Medical UniversityChongqing 400042China Institute of Life Sciences Chongqing Medical UniversityChongqing 400016China Experimental Teaching Administration Center Chongqing Medical UniversityChongqing 400016China

Urticaceae includes about 1300 species in 47 genera which largely spread in wet tropical regions,and 341 species in 2. genera are in *** species are used in chinese folk *** far,studies on chemistry and pharmacology of Urticaceous plants are mainly focused on nettle of Urtica *** this review,the chemical researches on 35 new compounds and related pharmacological effects of the plants in Urticaceae reported during 2.00-2.10 are *** 35 new compounds belong to the classes of lignan,secolignan,norlignan,flavonoid,alkaloid,sesquiterpenoid,triterpenoid,sterol,and *** main bioactivities include cytotoxic,antitumor,antimicrobial,antifungal,anti-BPH,anti-HIV,antidiabetic,hypolipidemic,5α-reductase inhibitory,hair regrowth promotion,and anti-oxidative activities.

关键词： bioactivities chemical constituents nettle Urtica L. Urticaceae

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中药治疗灼口综合征的临床观察

四川中医 2010年第8期28卷 108-109页

作者：吴国荣江苏省中医院口腔科江苏南京210029

目的:观察中药治疗灼口综合征的临床疗效。方法:将72.灼口综合征患者随机分为两组,治疗组(n=36)采用口服中药四逆散加味(柴胡、白芍、枳壳、甘草、酸枣仁、山萸肉、丹参、生地、麦冬、太子参)治疗,对照组(n=36)采用谷维素—核黄素—维... 详细信息

目的:观察中药治疗灼口综合征的临床疗效。方法:将72.灼口综合征患者随机分为两组,治疗组(n=36)采用口服中药四逆散加味(柴胡、白芍、枳壳、甘草、酸枣仁、山萸肉、丹参、生地、麦冬、太子参)治疗,对照组(n=36)采用谷维素—核黄素—维生素E联合方案治疗。结果:治疗组总有效率为86.11%,对照组总有效率为55.56%,治疗组明显优于对照组(P<0.01)。结论:中药治疗灼口综合征疗效较好,值得推广。

关键词：灼口综合征中医药疗法四逆散

重组人转化生长因子β_1对正畸牙模型大鼠牙齿移动和体重的影响

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中国新药与临床杂志 2011年第5期30卷 394-396页

作者：高海涛刘建国徐宇红江策管晓燕张疆弢丰雷曲阳医院口腔科上海200092 遵义医学院附属口腔医院贵州遵义563003

目的观察局部注射不同剂量重组人转化生长因子β_1(rhTGF-β_1)对正畸牙模型大鼠牙齿移动和体重的影响。方法建立正畸牙移动模型,30只模型大鼠按rhTGF-β_1的剂量随机分0(对照组)、1、5、10、50、100ng组,每组5只。将不同剂量rhTGF-β_1... 详细信息

目的观察局部注射不同剂量重组人转化生长因子β_1(rhTGF-β_1)对正畸牙模型大鼠牙齿移动和体重的影响。方法建立正畸牙移动模型,30只模型大鼠按rhTGF-β_1的剂量随机分0(对照组)、1、5、10、50、100ng组,每组5只。将不同剂量rhTGF-β_1每3d注射于模型大鼠左上颌第一磨牙颊侧牙龈黏膜下,10d后处死动物。测量牙齿移动距离,称量动物体重。结果实验组移动距离均大于对照组,1ng组牙齿移动距离较多,与对照组相比有显著差异;5ng组达峰值,与对照组相比有非常显著差异(P0.05)。各组动物处死时体重均轻于实验前,但各组间无显著差异(P>0.05)。结论低剂量rhTGF-β_1可加快正畸牙移动速度,高剂量对正畸牙移动速度无明显影响,且正畸牙移动过程中外源性TGF-β_1局部应用对大鼠体重无明显影响。

关键词：转化生长因子β1 正畸学矫正牙移动体重