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Formation of Superoxide radical and Hydrogen peroxide Enhanced by Trinitrotoluene in rat Liver, Brain, Kidney, and Testicle in Vitro and Monkey Liver in Vivo

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Biomedical and Environmental Sciences 1989年第1期2卷 72-77页

作者： KONG LiNGYUAN, JiANG QUANGUAN,~2.AND QU QiNGSHAN Department of Occupational health, School of public health, beijing medical university, beijing, china Department of Occupational health School of Public Health Beijing Medical University China.

Trinitrotoluene (TNT) increased the formation of adrenochrome from adrenaline and the formation of formaldehyde from methanol in rat liver mitochondria and microsomes in vitro as well as in monkey liver mitrochondria and microsomes in vivo. The effects were more prominent at higher TNT concentrations. These findings indicate that TNT enhances the production of superoxide radicals (O_2.-) and hydrogen peroxide (H_2._2.. The production of O_2.- was more prominent in systems containing added TNT than in those containing added benzyl viologen. H_2._2.production by mitochondria was more pronounced in the liver than in other organs, but its production by microsomes was more pronounced in the brain than in other organs. The results suggest that TNT undergoes cycling reduction which produces oxidative stress. 1989 Academic press, inc.

关键词： Brain Formation of Superoxide radical and Hydrogen peroxide Enhanced by Trinitrotoluene in rat Liver Kidney and Testicle in Vitro and Monkey Liver in Vivo

Effects of the CNTF-collagen gel-controlled delivery system on rat neural stem/progenitor cells behavior

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Science china(Life Sciences) 2010年第4期53卷 504-510页

作者： YANG ZhaoYang1, QiAO Hui2.& Li XiaoGuang1,3 1School of Biological Science and medical Engineering, Beihang university, {2. 100191, china 2.4} Heath School, Ningxia medical university, Yinchuan 750004, china 3{2. institute for Neuroscience, capital medical university, {2. 100069, china 1. School of Biological Science and medical Engineering Beihang University Beijing 100191 China2. Public Heath School Ningxia Medical University Yinchuan 750004 China3. Beijing Institute for Neuroscience Capital Medical University Beijing 100069 China

The injury of central nervous system (CNS) usually causes the cavity formation. Although transplantation of neural stem/precursor cells (NSpCs) into the lesioned area of CNS has been shown to be implicated in the functional restoration, the therapeutic result is limited by the poor survival of NSpCs as well as their insufficient proliferation and differentiation abilities. Type-1 collagen is considered as a candidate scaffold or drug delivery system to overcome the aforementioned obstacle. This study observed the effects of the CNTF (ciliary neurotrophic factor)-collagen gel-controlled delivery system and daily addition of soluble-form CNTF on the NSpC survival, migration, proliferation and differentiation. The results showed that, within 12.h of the initial co-culture, CNTF was released in a burst pattern, then the CNTF-collagen gel-controlled delivery system stably released CNTF for up to 12.d. The cell viability test, together with immunohistochemistry, rT-pCr and Western blotting, showed that the CNTF-collagen gel-controlled delivery system supported the NSpCs seeded on the surface of collagen gel survival and facilitated their migration and proliferation. The daily addition of soluble-form CNTF to the medium had similar effects to the CNTF-collagen gel-controlled delivery system, but large quantities of soluble-form CNTF were consumed during the entire process. Taken together, the CNTF-collagen gel-controlled delivery system not only provides a physical scaffold for the transplanted NSpCs to adhere and migrate, but also facilitates the NSpC survival, growth and proliferation, simultaneously reducing the consumption of the expensive growth factors. This system may be used to enhance the microenvironment in the lesioned area of CNS.

关键词： spinal cord neural stem/progenitor cells control release type-1 collagen CNTF

Frequent 3p2. Allelic Loss and Methylation-Associated rASSF1A inactivation in Non-Small Cell Lung Cancer and its Clinical implication

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Wuhan university Journal of Natural Sciences 2009年第5期14卷 457-464页

作者： ZHU Hong WONG Maria p Department of pathology Beijing Friendship Hospital Capital Medical University Beijing 100050 China Department of pathology Hong Kong University Hong Kong China

A total of 110 primary NSCLCs （non-small cell lung cancers） were recruited in this study to characterize the pattern of 3p2. LOH together with the rASSF1A methylation status and their clinical implication. 3p2. LOH by 8 microsatellite markers, rASSF1A methylation status by methylation-specific pCr （MSpCr） as well as bisulfite genomic sequencing （BGS）, and rASSF1A expression level by real-time quantitative pCr was performed. 3p2. LOH is frequent in NSCLC with a mean frequency of （41.2.3.7）%. Significant associations between 3p2. LOH and gender, smoking history, histological type, and tumor size were observed. Cases with LOH have a slightly lower rASSF1A expression than cases without LOH but not statistically significant. Comparison of rASSF1A methylation that resulted from the three analyses shows significant correlations from one another. Higher frequency of methylation was observed in larger tumors and in smokers compared with smaller tumors and non-smokers, respectively. A significant correlation was also observed in extent between methylation and rASSF1A expression, illustrating that epigenetic mechanism could affect gene expression. The significant clinicopathological relations of 3p2. LOH may be of great use for both early detection and therapeutic interventions.

关键词： non-small cell lung cancer loss of heterozygosity rAS association domain family iA gene (rASSF1A) methylation real-time quantitative pCr

传染病多点触发智慧化监测预警系统关键技术专家共识

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中华医学杂志 2024年第32期104卷 2995-3009页

作者：中国医学科学院北京协和医学院群医学及公共卫生学院北京大学公共卫生学院中国疾病预防控制中心传染病管理处四川大学华西公共卫生学院中国科学院地理科学与资源研究所首都医科大学附属北京地坛医院中华医学会公共卫生分会中国医学科学院北京协和医学院群医学及公共卫生学院北京大学公共卫生学院中国疾病预防控制中心传染病管理处四川大学华西公共卫生学院中国科学院地理科学与资源研究所首都医科大学附属北京地坛医院中华医学会公共卫生分会

有效的传染病监测预警系统是公共卫生安全体系的重要组成部分, 对预防与控制传染病暴发流行至关重要, 提升监测预警能力是推动疾病预防控制事业高质量发展的当务之急。专家组结合公共卫生与预防医学、临床医学、管理学、数据科学、计算... 详细信息

有效的传染病监测预警系统是公共卫生安全体系的重要组成部分, 对预防与控制传染病暴发流行至关重要, 提升监测预警能力是推动疾病预防控制事业高质量发展的当务之急。专家组结合公共卫生与预防医学、临床医学、管理学、数据科学、计算机科学等多学科专家的研究成果与实践经验, 经过反复多轮专家研讨, 形成了传染病多点触发智慧化监测预警系统关键技术专家共识。本共识内容主要包括传染病多点触发智慧化监测预警系统的相关概念和定义, 关键技术框架, 多渠道监测预警数据来源、获取与治理, 预警方法分类, 多点触发智慧化监测预警关键流程及路径, 多点触发预警及综合研判, 预警信号响应以及预警效果评价等方面, 以期为传染病多点触发智慧化监测预警系统建设及应用提供技术参考。

关键词：传染病监测预警多点触发智慧化

Factors influencing prehospital delay for patients with acute myocardial infarction

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Journal of Geriatric Cardiology 2007年第1期4卷 11-13页

作者： Shujuan CHENG Lufen GUO Juyuan LiU Xiaoling ZHU Hongbing YAN Emergency Department Beijing Anzhen Hospital Beijing 100029 China Emergency Department Beijing Anzhen Hospital Beijing 100029 China Public Health and Family Medicine Department Capital Medical University Beijing 100069 China Emergency Department Beijing Anzhen Hospital Beijing 100029 China Emergency Department Beijing Anzhen Hospital Beijing 100029 China

Objective To investigate the influencing factors for prehospital delay in patients with acute myocardial infarction (AMi). Methods A total of 807 consecutive patients with AMi who presented to the emergency department of beijing Anzhen Hospital were analyzed. The influence of several potential risk factors on the prehospital delay time (pDT) was evaluated by comparing patients admitted more than 2.hours after onset of chese pain with those admitted within 2.hours after onset. results Among 807 patients, 402.came to the hospital within 2.hours while the others arrived at the hospital after 2.hours. The median pDT was 130 min. Among the potential variables, advanced age, history of diabetes mellitus, occurrence of symptom at night and use of emergency medical service significantly affected pDT by multivariate analysis. Conclusion interventions aimed at reducing the prehospital delay in AMi should primarily focus on the awareness of the risk and help-seeking behavior of patients.

关键词： acute myocardial infarction emergency medical service risk factor prehospital delay time

Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis

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Acta pharmacologica Sinica 2004年第6期25卷 110-114页

作者： Yang XiANG2. Bing MA, Tao Li, Jun-wei GAO, He-ming YU3, Xue-jun Li4 Departmentof pharmacology, School of Basic medical Sciences, peking university, {2. 100083 2.ife Science institute, Nanjing university, Nanjing 2.0093 3National research institute for family planning, {2. 100081,china Department of pharmacology School of Basic Medical Sciences Peking University Beijing 100083 China

AiM: To study effects of acetazolamide on aquaporin-1 (AQp1) protein expression and angiogenesis. METHODS: Establishing Lewis-lung-carcinoma model, the localization of AQp1 in tumor tissues was investigated by immuno- histochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. rESULTS: immunohistochemical localization of AQp1in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAMand endothelial cell proliferation. CONCLUSiON: Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis.

关键词： neoplasms acetazolamide angiogenesis inhibitors CHip2.

Effects of Exposure to Trinitrotoluene on Male reproduction

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Biomedical and Environmental Sciences 1993年第2期6卷 154-160页

作者： Li Yi, JiANG QUAN-GUAN, YAO SHOU-Qi, LiU WEi, TiAN GAO-JUN,AND GUi JiNG-WEiDepartment of Occupational health, School of public health, beijing medical university, beijing ^Hebi institute of Occupational health, Henan province Anyang institute of Occupational health, Henan province Xinxiang institute of Occupational health, Henan province, china prof. Jiang Quan-guan, Department of Occupational health, School of public health, beijing medical university, beijing 100083 china. Department of Occupational health School of Public Health Beijing Medical University China.

A cross-sectional study was performed in 2.plants located in Henan province in 1990 for observing the reproductive and sexual functions of male workers exposed to trinitrotoluene (TNT). The TNT concentrations in the workplace air, except the packing site, were found to have exceeded MAC (1 mg/m3). TNT exposed male workers complained of more sexual disorders such as impotence, the loss of libido and sexual hypoesthesia than the control group. Compared with the control group, the volume of semen and percentage of motile spermatozoawere found to have significantly decreased, and the sperm malformation incidence increased significantly in TNT exposed workers. The serum testosterone content in TNT exposed male workers was significantly decreased too.

关键词： TNT

Xylene-induced Effects on Brain Neurotransmitters, Behavior and Fos protein in rats

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Biomedical and Environmental Sciences 1999年第2期12卷 116-124页

作者： WANG XiU-LUNG JiN Xi-pENG FU HUA CHEN Zi-QiNG DA CUi-Di HUANG XiAN-FEN DiNG BAO-QiNG (institute of Occupational medicine, Chinese Academy of preventive medicine,2. Nan Wei road, {2. 100050, china Deprtment of Occupational health, School of public health, Shanghai medical university, Shanghai 2.0032. china National Key Laboratory of Neurobiolegy, Shanghai medical university institute of Occupational Diseases, Yumen Oil Administration, Gansu province, china) institute of Occupational medicine Chinese Academy of Preventive Medicine Beijing Chin

Male Sprague-Dawley rats administered xylene intrapetitoneally on alternate days at a dose of 12. or 2.0mg/kg for 30 days exhibited no marked changes in locomotor activity, learning and memory capacity. However in rats given xylene on alternate day at a dose of 500 mg/kg for 30 days, a significant decrease in locomotor activity, deficits in leaming ability and memory loss were detected. These xylene-induced behavioral ehanges were assoryiated with a decrease in fyendorphin and leuenkaphlin concentrations in the pons-medulla. On the contrary, xylene at a dose of 500mg/kg increased the β-endorphin level in caudate and c-fos expression in hippocampus. These data suggest that the xylene-induced behavioral alterations might be associated with the expression of Fos protein in the hippocampus.

关键词： Behavior and Fos protein in rats Xylene-induced Effects on Brain Neurotransmitters Fos

Overview of HBV whole genome data in public repositories and the Chinese HBV reference sequences

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progress in Natural Science:Materials international 2008年第1期18卷 13-20页

作者： Guanghua Wu a,b, Huiguo Ding c, Changqing Zeng b,* a Graduate School of Chinese Academy of Sciences, beijing 100049, china b beijing institute of Genomics, Chinese Academy of Sciences, beijing 101300, china c beijing Youan Hospital, capital medical university, beijing 100069, china Graduate School of Chinese Academy of Sciences Beijing 100049 China beijing institute of Genomics Chinese Academy of Sciences Beijing 101300 China beijing Youan Hospital Capital Medical University Beijing 100069 China

The number of Hepatitis B virus (HBV) whole genomic sequences in public nucleotide databases (GenBank, EMBL, and DDBJ) had reached 866 by January 1, 2.07. Coming from 46 countries and regions, these sequences were categorized as eight genotypes (A–H). With the statistical and phylogenetic analysis on all available complete genomic data of HBV, we here present an overview of HBV sequences in public databases. From all registered 2.9 HBV genomes in Chinese regions as well as 59 sequencing data from our research group, we report the establishment of reference sequences of HBV strains prevailing in {2.. These analyses provide clues for the effects of HBV genotypes in host clinical progressions, geographic distribution of the infection, and the viral evolutionary history. Moreover, the viral sequence reference would be helpful in the identiffcation of various HBV mutations. Based on the analysis of various public databases, we suggest that the Chinese HBV database with the clinical information should be constructed.

关键词： public databases HBV genomic sequences Genotype Subtype Chinese reference sequences