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A seed-specific AP2.domain transcription factor from soybean plays a certain role in regulation of seed ger-mination

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Science china(Life sciences) 2008年第4期51卷 336-345页

作者： WANG ChunMei, WANG HuiWen, ZHANG JinSong & CHEN ShouYi National Key Laboratory of Plant genomics, institute of Genetics and Developmental Biology, {2. academy of sciences, Bei-jing 100101, china National Key Laboratory of Plant genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China

Plant seed development and germination are under strict temporal and spatial regulation, and tran-scription factors play important roles in this regulation. In the present study we identified an EST ex-pressed specifically in the developing soybean seeds. The full length of the gene was obtained through further RACE analysis and the gene was named GmSGR. Sequence analysis revealed that this gene belonged to the AP2.ERF transcription factor family. Its AP2.domain had the highest similarity with that of the A-3 member AtABI4 of DREB subgroup in the AP2.ERF family in Arabidopsis. GmSGR did not exhibit transcriptional activation activity in the yeast assay system. GmSGR was overexpressed in Arabidopsis and the germination rates of the transgenic seeds were significantly higher than that of the wild type seeds under higher concentrations of ABA and glucose respectively. However, the germina-tion rates of the transgenic seeds were lower than that of control under salt stress. The expression of AtEm6 and AtRD2.B was higher in the seedlings of the transgenic plants than that in the wild-type seedlings. These results suggest that GmSGR may confer reduced ABA sensitivity and enhanced salt sensitivity to the transgenic seeds through regulating the expression of AtEm6 and AtRD2.B genes.

关键词： ABA AP2.ERF transcription factor salt stress seed germination soybean

Cytogenetic comparisons between A and G genomes in Oryza using genomic in situ hybridization

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Cell Research 2006年第3期16卷 260-266页

作者： Zhi Yong Xiong~(1,2. Guang Xuan Tan~1 Guang Yuan He~2.Guang Cun He~1 Yun Chun Song~11 Key Laboratoty of the Ministry of Education for Plant Developmental Biology,College of Life sciences,Wuhan University,Wuhan 430072.china.2.china-UK HUST-R Res Genetic Engineering and genomics Joint Laboratory,Huazhong University of Science and Technology,Wuhan 430074,china Key Laboratory of the Ministry of Education for Plant Developmental Biology College of Life Sciences Wuhan University Wuhan China china-UK HUST-R Res Genetic Engineering and genomics Joint Laboratory Huazhong University of Science and Technology Wuhan China

The genomic structures ofOryza sativa （A genome） and *** （G genome） were comparatively studied usingbieolor genomic in situ hybridization （GISH）.GISH was clearly able to discriminate between the chromosomes of *** and *** in the interspecific F1 hybrids without blocking DNA,and co-hybridization was hardly *** average mitotic chromosome length of *** was found to be 1.69 times that of ***.A comparison of4,6-diamidino-2.phenylindole staining showed that the chromosomes of *** were more extensively labelled,suggesting that the G genome is amplified with more repetitive sequences than the A *** interphase nuclei,9-12.hromocenters were normally detected and nearly all the chromocenters constituted the G genome-specific *** larger chromocenters formed by chromatin compaction corresponding to the G genome were detected in the hybridcompared with its *** pachytene of the F1 hybrid,most chromosomes of A and G did not synapse eachother except tot 1-2.chromosomes paired at the end of their *** meiotic metaphase I,three types of chromosomalassociations,i.e.O,sativa-O,sotiva（A-A）,***-O,meyeriana （A-G） and ***-O,meyeriana （G-G）,wereobserved in the F1 *** A-G chromosome pairing configurations included bivalcnts and *** resultsprovided a foundation toward studying genome organization and evolution of ***.

关键词： Oryza sativa Oryza meyeriana 45S rDNA genomic in situ hybridization

Plant DNA barcoding in china

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Journal of Systematics and Evolution 2011年第3期49卷 165-168页

作者： 1De-Zhu LI 2.ian-Quan LIU 3Zhi-Duan CHEN 1Hong WANG 4Xue-Jun GE 3Shi-Liang ZHOU 1Lian-Ming GAO 5Cheng-Xin FU 6Shi-Lin CHEN 1(Plant Germplasm and genomics Centre,Germplasm Bank of Wild Species in Southwest china Key Laboratory of Biodiversity and Biogeography,Kunming {2. of Botany,chinese academy of sciences,Kunming 6502.4,china) 2.Key Laboratory of Arid and Grassland Ecology,College of Life sciences,Lanzhou University,Lanzhou 730000,china) 3(State Key Laboratory of Systematic and Evolutionary Botany,{2. of Botany,chinese academy of sciences,Beijing 100093,china) 4(Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South china Botanical Garden,chinese academy of sciences,Guangzhou 510650,china) 5(Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education,College of Life sciences,Zhejiang University,Hangzhou 310058,china) 6({2. of Medicinal Plant Development,chinese academy of Medical sciences,Peking Union Medical College,Beijing 100193,china) (Plant Germplasm and genomics Centre Germplasm Bank of Wild Species in Southwest China Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Key Laboratory of Arid and Grassland Ecology College of Life Sciences Lanzhou University Lanzhou 730000 China) (State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing 100093 China) (Key Laboratory of Plant Resources Conservation and Sustainable Utilization South China Botanical Garden Chinese Academy of Sciences Guangzhou 510650 China) (Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education College of Life Sciences Zhejiang University Hangzhou 310058 China) (institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100193 China)

Identification is the keystone of biology(Bell, 1986).However,to biologists and students of biology, the total numbers of species that must be identified far outnumber the names commonly used in English,chinese,or oth... 详细信息

关键词： DNA Plant DNA barcoding in china matK

Finding Signals for Plant Promoters

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genomics, Proteomics & Bioinformatics 2003年第1期1卷 68-73页

作者： Weimou Zhenginstitute of Theoretical Physics, chinese {2. of sciences, Beijing 100080, china Beijing genomicsinstitute, chinese {2. of sciences, Beijing 1013.0, china institute of Theoretical Physics Chinese Academy of Sciences Beijing 100080 China Beijing genomics institute Chinese Academy of Sciences Beijing 101300 China

The strongest signal of plant promoter is searched with the model of single motif with two types. It turns out that the dominant type is the TATA-box. The other type may be called TATA-less signal, and may be used in gene finders for promoter recognition. While the TATA signals are very close for the monocot and the dicot, their TATA-less signals are significantly different. A general and flexible multi-motif model is also proposed for promoter analysis based on dynamic programming. By extending the Gibbs sampler to the dynamic programming and introducing temperature, an efficient algorithm is developed for searching signals in plant promoters.

关键词： for on is as et Pro Finding Signals for Plant Promoters been of into

A new synonym of Pedicularis cyathophylla(Orobanchaceae)

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Journal of Systematics and Evolution 2011年第5期49卷 507-507页

作者： 1,2.Wen-Bin YU 1,2.Pan-Hui HUANG 1~Hong WANG 1(Key Laboratory of Biodiversity and Biogeography,and Plant Germplasm and genomics Center,Kunming institute of Botany,{2. academy of sciences,Kunming 6502.4,china) 2.Graduate University of {2. academy of sciences,Beijing 100049,china) (Key Laboratory of Biodiversity and Biogeography and Plant Germplasm and Genomics Center Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China) (Graduate University of chinese academy of {2. Beijing 100049 China)

Pedicularis cyathophylla Franchet in Bull. Soc. Bot. France 47: ***: china. Sichuan: Kangding, Zheduoshan Mountains(Ta-tsien-kou & Tche-to-chan), J. A. Soulie′ 2.8 (holotype,P0016082., P!).Pedicularis xiangchengensis... 详细信息

关键词： PE A new synonym of Pedicularis cyathophylla Orobanchaceae

Characterization of Arabidopsis calreticulin mutants in response to calcium and salinity stresses

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Progress in Natural Science:Materials International 2008年第10期18卷 1219-1224页

作者： Zhigang Li Yangrong Cao Jinsong Zhang Shouyi Chen institute of Genetics and Developmental Biology,chinese {2. of sciences,National Key Laboratory of Plant genomics,Chaoyang District,Datun Road,Beijing 100101,china institute of Genetics and Developmental Biology Chinese Academy of Sciences National Key Laboratory of Plant Genomics Chaoyang District Datun Road Beijing 100101 China

As an important calcium-binding protein,calreticulin plays an important role in regulating calcium homeostasis in endoplasmic retic- ulum (ER) of ***,we identified three loss-of-function mutants of calreticulin genes in Arabidopsis to demonstrate the function of calreticulin in response to calcium and salinity *** are three genes encoding calreticulin in Arabidopsis,and they are named AtCRT1,2.and 3,*** found that both single mutant of crt3 and double mutant of crtl crt2.were more sensitive to low calcium environment than wild-type ***,crt3 mutant showed more sensitivity to salt treatment at germination stage, but tolerance to salt stress at later stage compared with wild-type ***,there was no obvious growth difference in the mutant crtl and crt2.compared with wild-type Arabidopsis under calcium and salt *** results suggest that calreticulin functions in plant responses to calcium and salt stresses.

关键词： Calreticulin Calcium Stress Arabidopsis

应用FP-TDI技术进行高通量单核苷酸多态分型

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生物化学与生物物理进展 2004年第8期31卷 731-735页

作者：罗春清邓立彬周珺林伟曾长青中国科学院基因组学研究所暨北京华大基因研究中心

FP TDI (fluorescencepolarizationtemplate directeddye terminatorincorporation)是一种操作简单、实验投入少、适于高通量反应的单核苷酸多态等位基因分型技术 .使用两种评价分型图像质量的数值指标 ,可以有效地对分型结果进行评价 ... 详细信息

FP TDI (fluorescencepolarizationtemplate directeddye terminatorincorporation)是一种操作简单、实验投入少、适于高通量反应的单核苷酸多态等位基因分型技术 .使用两种评价分型图像质量的数值指标 ,可以有效地对分型结果进行评价 ,使该技术得到了改进 .在此基础上优化了实验条件 ,并应用该技术 ,对人类基因组 3号染色体上随机选取的 337个单核苷酸多态性位点进行了高通量分型 ,反应的一次成功率达到 5 9 94 % .

关键词：基因分型单核苷酸多态遗传标记功能基因组学 SNP 荧光偏振结合模板指导的染料掺入

T-DNA标签在转基因水稻基因组中的整合特点

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生物化学与生物物理进展 2004年第10期31卷 912-917页

作者：李述田田颖川何朝族中国科学院微生物研究所

利用热不对称交错PCR (TAIL PCR) ,对 2.0 0个含T DNA插入的转基因水稻株系进行分析 ,获得了 15 9个T DNA右边界侧翼序列 .其中 ,92.序列含有T DNA右边界和侧邻的水稻基因组序列 ,78个序列与已公布的水稻BAC/PAC克隆有 97%～ 10 0 %的... 详细信息

利用热不对称交错PCR (TAIL PCR) ,对 2.0 0个含T DNA插入的转基因水稻株系进行分析 ,获得了 15 9个T DNA右边界侧翼序列 .其中 ,92.序列含有T DNA右边界和侧邻的水稻基因组序列 ,78个序列与已公布的水稻BAC/PAC克隆有 97%～ 10 0 %的同源性 ,从而可作为T DNA标签定位在水稻的 12.染色体上 .结合先前定位的 16 9个T DNA标签 ,对T DNA在水稻基因组中的整合特点进行了分析 .结果发现 ,在T DNA右边界和侧邻的水稻基因组序列的连接处 ,14 6 %的T DNA标签含有 3～ 74bp的填充序列 .在不含填充序列的连接处 ,2.1 3%的T DNA标签 ,在整合后的T DNA右边界与侧翼的水稻基因组序列之间显示出 3～ 5bp的微同源性 .填充序列和微同源性的存在 ,揭示了T DNA在水稻基因组中的整合既存在双链断裂修补机制 ,又存在单链裂缝修补机制 .T DNA倾向于整合到富含A/T核苷酸的基因组区域 ,即主要在基因的 5′和 3′端调控区以及内含子中 .

关键词：水稻(Oryza sativa L.)基因组 T-DNA标签整合特点

A Scenario on the Stepwise Evolution of the Genetic Code

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genomics,Proteomics & Bioinformatics 2007年第Z1期 143-151页

作者： Jing-Fa Xiao and Jun Yu CAS Key Laboratory of Genome sciences and Information,Beijing institute of {2.,chinese academy of sciences,Beijing 10002.,china.

It is believed that in the RNA world the operational (ribozymes) and the infor-mational (riboscripts) RNA molecules were created with only three (adenosine,uridine,and guanosine) and two (adenosine and uridine) nucleosides,respectively,so that the genetic code started uncomplicated. Ribozymes subsequently evolved to be able to cut and paste themselves and riboscripts were acceptive to rigor-ous editing (adenosine to inosine); the intensive diversification of RNA molecules shaped novel cellular machineries that are capable of polymerizing amino acids-a new type of cellular building materials for life. Initially,the genetic code,encoding seven amino acids,was created only to distinguish purine and pyrimidine; it was later expanded in a stepwise way to encode 12.15,and 2. amino acids through the relief of guanine from its roles as operational signals and through the recruitment of cytosine. Therefore,the maturation of the genetic code also coincided with (1) the departure of aminoacyl-tRNA synthetases (AARSs) from the primordial translation machinery,(2. the replacement of informational RNA by DNA,and (3) the co-evolution of AARSs and their cognate tRNAs. This model predicts gradual replacements of RNA-made molecular mechanisms,cellular processes by proteins,and informational exploitation by DNA.

关键词： genetic code codon aminoacyl-tRNA synthase GC content