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流感疫苗单向免疫扩散试验中抗血清制备新方法的研究

第五次全国免疫诊断暨疫苗学术研讨会

作者： Qiang Liu~(1,2),Yue Li~3,GuiBo Yang~1,JieJie Dai~4,Ruth M Ruprecht~(5,6),YiMing Shao~ 1 national Center for AIDS/STD control and prevention,china CDC,beijing,china 2 national institutes for food and drug control,beijing,china 3 College of Lifesciences,Nankai University,Tianjin,china 4 Institute of Medical Biology,CAMS,Kuming,china 5 Dana-Farber Cancer Institute,Boston,Massachusetts, USA 6Harvard Medical School,Boston,Massachusetts,USA

The increasing prevalence of human immunodeficiency virus type 1(HIV-1) subtype C infection worldwide calls for efforts to develop a relevant animal model for evaluating AIDS candidate *** china,the prevalent HIV strains comprise a circulating recombinant form,BC(CRF07C),in which the envelope belongs to subtype *** evaluate potential AIDS vaccines targeting Chinese viral strains in nonhuman primate models,we constructed a simian-human immunodeficiency virus(SHIV) carrying most of the envelope sequence of a primary HIV-1 clade C strain isolated from an HIV-positive intravenous drug user from YunNan province in *** of six Chinese rhesus macaques with SHIV-CN97001 resulted in a low level of viremia and no significant alteration in CD4+T-lymphocyte *** determine whether in vivo adaptation would enhance the infectivity of SHIV-CN97001,the parental infectious strain was serially passaged through eight Chinese rhesus *** hallmarks of SHIV infectivity developed gradually, as shown by the increasingly elevated peak viremia with each *** findings establish that the R5-tropic SHIV-CN97001 /Chinese rhesus macaque model should be very useful for evaluation of HIV-1 subtype C vaccines in china.

关键词： Clade C Passage Rhesus macaques SHIV

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微生物学免疫学进展 2011年第4期39卷 8-12页

作者：顾琴李娟徐康维邵铭廖国阳李卫东李长贵中国医学科学院医学生物学研究所昆明650118 中国食品药品检定研究院北京100050

流感疫苗血凝素含量检测方法为单向免疫扩散试验,其抗血清通常由WHO参比实验室提供,通过纯化病毒、蛋白酶切获得血凝素主要抗原片段,然后再免疫动物获得抗血清。该方法制备时间较长,是制约流感疫苗研发及检测的主要因素。以RT-PCR方法... 详细信息

流感疫苗血凝素含量检测方法为单向免疫扩散试验,其抗血清通常由WHO参比实验室提供,通过纯化病毒、蛋白酶切获得血凝素主要抗原片段,然后再免疫动物获得抗血清。该方法制备时间较长,是制约流感疫苗研发及检测的主要因素。以RT-PCR方法获取甲型H1N1流感病毒血凝素中主要抗原片段基因,构建重组质粒并在大肠杆菌中表达,表达产物经纯化、复性后,以蛋白电泳及免疫印迹方法进行了鉴定。以纯化蛋白免疫家兔,制备了相应的抗体,初步证明可用于常规的单向免疫扩散试验。以该法可以快速获得抗血清,尤其是在大流行流感疫苗的研发中,加快疫苗研发进程。

关键词：流感病毒血凝素重组表达单向免疫扩散试验

胸部X线(正位)肺结节数据集构建与质量控制专家共识

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中华放射学杂志 2024年第4期58卷 375-380页

作者：中华医学会放射学分会中国食品药品检定研究院李静莉刘士远刘凯王浩不详中国食品药品检定研究院医疗器械检定所北京102629 海军军医大学长征医院放射诊断科上海200003 中国食品药品检定研究院医疗器械检定所光机电室

采用人工智能进行肺结节辅助检测已经在医疗器械领域成为成熟的应用,这一技术可以有效地帮助影像医师快速准确地发现肺结节,从而提高体检和肺癌筛查在肺癌防治中的效率和效果。胸部X线(正位)在肺结节检测中具有辐射剂量低、检查速度快... 详细信息

采用人工智能进行肺结节辅助检测已经在医疗器械领域成为成熟的应用,这一技术可以有效地帮助影像医师快速准确地发现肺结节,从而提高体检和肺癌筛查在肺癌防治中的效率和效果。胸部X线(正位)在肺结节检测中具有辐射剂量低、检查速度快、设备资源分配充分以及检查费用低等特点。随着影像组学和人工智能技术在胸部X线(正位)领域的逐渐应用,数据标注和质量控制对于人工智能算法和产品的发展起到了至关重要的作用。本专家共识旨在为胸部X线(正位)肺结节数据的采集、标注以及质量控制提供指导意见,促进胸部X线(正位)肺结节标准化标注数据集的建设,为人工智能算法和产品的训练、调优和测试提供重要的依据,从而推动医学影像人工智能技术的快速发展以及进一步实现临床应用。

关键词：检查速度检查费用人工智能技术影像组学肺癌筛查肺结节人工智能算法医疗器械

Traditional Chinese medicine in treatment of opiate addiction

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Acta Pharmacologica Sinica 2006年第10期27卷 1303-1308页

作者： Jie SHI~2 Yan-li LIU~3 Yu-xia FANG~4 Guo-zhu XU~2 Hai-fen ZHAI~2 Lin LU~(2,5,6) ~2national Institute on drug Dependence,Peking University,beijing 100083,china ~3College of Pharmacy,Soochow University,Suzhou 215123,china ~4Clinical Pharmacology and Therapeutics Branch,Intramural Research Program.national Institute on drug Abuse,national institutes of Health,Baltimore,Maryland 21224,USA.5Behavioral Neuroscience Branch,Intramural Research Program,national Institute on drug Abuse,national institutes of Health,Baltimore,Maryland 21224,USA national Institute on drug Dependence Peking University Beijing 100083 China. linlu@***.

Traditional Chinese medicine (TCM) includes Chinese medicine and *** medicine consists of natural products including plants,animals *** has been practiced in china for more than 2000 years,and for thepast 200 years has been used in treatment of drug *** Chinese medi-cines for the treatment of opiate addiction have been approved by the ChineseState food and drug Administration (SFDA),and at least 6 are in clinical *** general therapeutic principle of Chinese medicine developed was based on itsunique theory of"reinforcing healthy Qi and resolving and removing effects oftoxicity".Acupuncture,another essential part of TCM,which was developedbased on the principle that"functions of the human body are controlled by the‘Jing-Luo’and‘Qi-Xue’system",has been used not only in china,but also inEurope,the USA and other countries,for controlling opiate *** aresome advantages in using TCM for opiate detoxification,including less harmfulside effects,high safety and ideal effects in the inhibition of protracted with-drawal symptoms and ***-administration of TCM with modern medicineshows some synergistic effects in *** TCM for detoxificationalso have efficacy in the rehabilitation of abnormal body functions induced bychronic drug use,including improving immune function,increasing working memoryand preventing neurological *** that TCM is effective in the preven-tion of relapse and causes fewer side effects,it may be used widely in the treat-ment of opiate addiction.

关键词： Chinese medicine acupuncture opiate addiction withdrawal relapse treatment

Investigation of integrons/cassettes in antimicrobial-resistant Escherichia coli isolated from food animals in china

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Science china(Life Sciences) 2010年第5期53卷 613-619页

作者： SONG Li1,NING YiBao1,SHEN JianZhong2,FAN XueZheng,ZHANG ChunPing1,YANG ChengHuai1 & HAN JianFeng3 1 china Institute of Veterinary drug control,beijing 100081,china 2 china Agricultural University,beijing 100094,china 3 china Center for Disease control and Prevention,beijing 100050,china 1. china Institute of Veterinary drug control Beijing 100081 China2. China Agricultural University Beijing 100094 China3. China Center for Disease Control and Prevention Beijing 100050 China

In this study,326 Escherichia coli isolates from food animals collected during the last four decades in china were characterized using antimicrobial susceptibility testing and screening for integrons/*** inhibitory concentration(MIC) testing indicated that the antimicrobial resistance of *** has increased since the *** findings of this study present a warning to veterinary practitioners about the excessive use of antimicrobials,and suggest the necessity for surveillance and control of antimicrobial resistance in veterinary clinical medicine in china.

关键词： E.coli Antimicrobial resistance integron/cassette last four decades

头颈动脉CT血管成像数据标注与质量控制专家共识

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中华放射学杂志 2024年第2期58卷 150-157页

作者：中华医学会放射学分会医学影像人工智能工作组北京医学会放射学分会人工智能学组、中国食品药品检定研究院刘士远陈敏金征宇卢洁周俊林李小虎萧毅郑敏文张苗王浩不详海军军医大学第二附属医院放射诊断科上海200003 北京医院放射科北京100730 中国医学科学院、北京协和医学院、北京协和医院放射科北京100730 首都医科大学宣武医院放射与核医学科北京100053 兰州大学第二医院放射科兰州730030 安徽医科大学第一附属医院医学影像科空军军医大学西京医院放射诊断科中国食品药品检定研究院医疗器械检定所

人工智能使头颈动脉CT血管成像(CTA)的高效、快捷重建及诊断成为可能, 但决定算法精度及泛化性的前提是有大量的高质量、高一致性的标注数据。为促进人工智能技术在头颈动脉CTA中的开发与应用, 使辅助重建及诊断技术符合临床工作流程及... 详细信息

人工智能使头颈动脉CT血管成像(CTA)的高效、快捷重建及诊断成为可能, 但决定算法精度及泛化性的前提是有大量的高质量、高一致性的标注数据。为促进人工智能技术在头颈动脉CTA中的开发与应用, 使辅助重建及诊断技术符合临床工作流程及准确性要求, 中华医学会放射学分会医学影像人工智能工作组、北京医学会放射学分会人工智能学组、中国食品药品检定研究院在头颈动脉CTA数据集的构建、数据标注规则及质量控制等方面达成专家共识, 有助于促进行业内部统一认知, 提升数据标签的一致性, 优化数据集质量, 促进人工智能辅助诊断技术在头颈动脉CTA中的应用和发展。

关键词：颈动脉脑动脉 CT血管成像人工智能数据标注

Centrophenoxine improves chronic cerebral ischemia induced cognitive deficit and neuronal degeneration in rats

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Acta Pharmacologica Sinica 2004年第12期25卷 24-30页

作者： Yun LIAO2, Rui WANG, Xi-can TANG3 State KeyLaboratory of drug Research, Shanghai Institute of Materia Medica, Shanghai institutes for Biological Sciences, Chinese AcademyofSciences, Shanghai 201203, china 2TongjiMedical College of Huazhong Universityof Science & Technology, Wuhan 430030, china Tongji Medical College of Huazhong University of Science & Technology Wuhan 430030 China

AIM: To study the effects of centrophenoxine (CPH, meclofenoxate) on chronic cerebral hypoperfusion induced deficits in rats. METHODS: Chronic hypoperfusion in rats was performed by permanent bilateral ligation of the common carotid arteries. Morris water maze was used to measure spatial memory performance. Spectrophoto- metrical techniques were used to assay SOD, GPx activities, MDA content, TXB2, and 6-keto-PGF1α levels. Mor- phological change was examined by HE staining. The expression of Bax and p53 protein were assayed by immuno- histochemistry analysis. RESULTS: Chronic hypoperfusion in rats resulted in spatial memory impairments shown by longer escape latency and shorter time spent in the target quadrant. These behavioral dysfunction were accom- panied by increase in SOD and GPx activities, the content of MDA, the levels of pro-inflammatory mediators (TXB2, 6-keto-PGF1α), overexpression of Bax and P53 protein, and delayed degeneration of neurons in cortex and hippocampus. Oral administration of CPH (100 mg/kg, once per day for 37 d) markedly improved the memory impairment, reduced the increase in antioxidant enzyme activities, MDA content and the levels of pro-inflammatory mediators to their normal levels, and attenuated neuronal damage. CONCLUSION: The abilities of CPH to attenu- ate memory deficits and neuronal damage after ischemia may be beneficial in cerebrovascular type dementia.

关键词： meclofenoxate brain ischemia free radicals maze learning TXB2 6-keto-PGF1α Bax protein p53 protein

Genomic research for important pathogenic bacteria in china

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Science china(Life Sciences) 2009年第1期52卷 50-63页

作者： YANG RuiFu1,GUO XiaoKui2,YANG Jian3,JIANG YongQiang1,PANG Bo4,CHEN Chen5,YAO YuFeng2,QIN JinHong2 & LI QingTian2 1 Laboratory of Analytical Microbiology,State Key Laboratory of Pathogen and Biosecurity,beijing Institute of Microbiology and Epidemiology,beijing 100071,china 2 Department of Medical Microbiology and Parasitology,institutes of Medical Sciences,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,china 3 State Key Laboratory for Molecular Virology and Genetic engineering,Institute of Pathogen Biology,Chinese Academy of Medical Sciences,beijing 100176,china 4 State Key Laboratory for Infectious Disease Prevention and control,Institute for Communicable Disease control and Prevention,Chinese Center for Disease control and Prevention,beijing 102206,china 5 beijing Genomics Institute at Shenzhen,Shenzhen 518083,china 1. Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China 2. Department of Medical Microbiology and Parasitology Institutes of Medical Sciences Shanghai Jiao Tong University School of Medicine Shanghai 200025 China 3. State Key Laboratory for Molecular Virology and Genetic engineering Institute of Pathogen Biology Chinese Academy of Medical Sciences Beijing 100176 China 4. State Key Laboratory for Infectious Disease Prevention and control Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China 5. beijing Genomics Institute at Shenzhen Shenzhen 518083 China

Rapid accumulation of bacterial genomic data offered an unprecedented opportunity to understand bacterial biology from a holistic view of *** can thus closely look at the way in which a pathogen is evolved,and these data has been applied to molecular epidemiology and microbial forensics,and screening of novel diagnostic,vaccine and drug *** newly developed high-throughput low-cost sequencing technologies,such as 454,Solexa and SOLiD,will promote the acquisition and application of genomic data in new research areas that we dared not imagine previously,such as the metagenomics of human gastric-intestinal tract,for better and comprehensive understanding of human health and disease.

关键词： genome pathogenic bacteria sequencing application

冠状动脉CT血管成像数据标注和质量控制专家共识

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中华放射学杂志 2024年第1期58卷 25-33页

作者：中华医学会放射学分会心胸学组中国医师协会放射医师分会心血管学组中国食品药品检定研究院刘士远金征宇郑敏文吕滨萧毅李小虎卢洁张龙江王浩张佳胤不详海军军医大学第二附属医院放射诊断科上海200003 中国医学科学院北京协和医学院北京协和医院放射科北京100730 空军军医大学西京医院放射诊断科西安710032 中国医学科学院北京协和医学院国家心血管病中心阜外医院放射影像科北京100037 安徽医科大学第一附属医院医学影像科首都医科大学宣武医院放射与核医学科解放军东部战区总医院放射诊断科中国食品药品检定研究院医疗器械检定所上海市第一人民医院放射影像科

冠状动脉CT血管成像(CCTA)是冠状动脉疾病诊断的首选影像检查。使用人工智能技术对CCTA数据进行图像重组、斑块评估和血管分析,是人工智能医疗器械发展的重要方向,为临床机构提供了全新的影像后处理和诊断方式。影像人工智能产品的生产... 详细信息

冠状动脉CT血管成像(CCTA)是冠状动脉疾病诊断的首选影像检查。使用人工智能技术对CCTA数据进行图像重组、斑块评估和血管分析,是人工智能医疗器械发展的重要方向,为临床机构提供了全新的影像后处理和诊断方式。影像人工智能产品的生产研发、验证确认、临床质控等活动均需要依托图像数据的高质量标注结果。为了规范CCTA的数据标注,本共识对CCTA的标注提出统一的共识和指导意见,从数据集适用范围和定义、数据要求、标注流程及标注规则等各个方面阐述专家组就该问题达成的共识,旨在帮助业内形成统一认知,提高CCTA数据的标注质量,推动相关数据集的建设,促进CCTA相关的人工智能研究规范化发展,提升冠状动脉疾病的诊疗水平。

关键词：体层摄影术,X线计算机冠状动脉CT血管成像数据标注质量控制

Synthesis of N^(11)-anchoring biotinylated artemisinin derivatives and their preliminary biological assessment

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Science china Chemistry 2010年第1期53卷 119-124页

作者： PAN QiYu1,2,WANG ShaoZhong1,LU JinJian3,MENG LingHua3 & YAO ZhuJun1,2 1Nanjing national Laboratory of Microstructures(NNLM),School of Chemistry and Chemical Engineering,Nanjing University,Nanjing 210093,china 2Joint Laboratory of Green Chemistry,Department of Chemistry,University of Science and Technology of china,Hefei 230026,china 3State Key Laboratory of drug Research,Shanghai Institute of Materia Medica,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 201203,china. 1. Nanjing national Laboratory of Microstructures (NNLM) School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China2. Joint Laboratory of Green Chemistry Department of Chemistry University of Science and Technology of China Hefei 230026 China3. State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 201203 China

Unique endoperoxide moiety of artemisinin and its derivatives has been considered the functionality exhibiting highly potent antimalarial and anticancer *** investigate the mechanisms of their biological actions,development of suitable molecular probes including biotinylated derivatives is of extreme *** synthesis and preliminary biological assessment of four new biotinylated artemisinin derivatives have been reported in this work.

关键词： artemisinin endoperoxide biotinylated derivative synthesis anticancer activity