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Sodium ferulate prevents amyloid-beta-induced neurotoxicity through suppression of p38 MApK and upregulation of ErK-1/2 and Akt/protein kinase B in rat hippocampus

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Acta pharmacologica Sinica 2005年第8期26卷 943-951页

作者： Ying JIN~2, En-zhi YAN~3, Ying FAN~3, Zhi-hong ZONG~4, Zhi-min QI~3, Zhi LI~(2,5) ~2Department of Ethnopharmacology, china medical university, Shengyang 110001, china ~3Department of pharmacology, Jinzhou medical college, Jinzhou 121001, china ~4Department of Biochemistry, china medical university Shengyang 110001, china Department of Ethnopharmacology China Medical University Shengyang China Department of pharmacology Jinzhou Medical College Jinzhou China Department of Biochemistry China Medical University Shengyang China

Aim: To observe whether an amyloid β （Aβ）-induced increase in interleukin （IL）-1β was accompanied by an increase in the p38 mitogen-activated protein kinase （MApK） pathway and a decrease in the cell survival pathway, and whether so-dium ferulate （SF） treatment was effective in preventing these Aβ-induced changes. Methods: rats were injected intracerebroventricularly with Aβ25-35. Seven days after injection, immunohistochemical techniques for glial fibrillary acidic protein （GFAp） were used to determine the astrocyte infiltration and activation in hippo-campal CA1 areas. The expression of IL-1β, extracellular signal-regulated kinase （ErK）, p38 MApK, Akt/protein kinase B （pKB）, Fas ligand and caspase-3 were determined by Western blotting. The caspase-3 activity was measured by clea-vage of the caspase-3 substrate （Ac-DEVD-pNA）. reverse transcription-polymerase chain reaction was used to analyze the changes in IL- 1βmrNA levels. results:Intracerebroventricular injection of Aβ-（25-35） elicited astrocyte activation and infiltration and caused a strong inflammatory reaction characterized by increased IL-1β production and elevated levels of IL-1β mrNA. Increased IL-1β synthesis was accompanied by increased activation of p38 MApK and downregulation of phospho-ErK and phospho-Akt/pKB in hippocampal CA regions prepared from Aβ-treated rats, leading to cell death as assessed by activa-tion of caspase-3. SF significantly prevented Aβ-induced increases in IL-Iβ and p38 MApK activation and also Aβ-induced changes in phospho-ErK and phospho-Akt/pKB expression levels. Conclusion: SF prevents Aβ-induced neu-rotoxicity through suppression of p38 MApK activation and upregulation of phospho-ErK and phospho-Akt/pKB expression.

关键词： ferulic acid amyloid interleukin-1 p38 mitogen-activated protein kinases extra-cellular signal-regulated kinases proto-oncogene proteins c-akt Alzheimer disease

Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide

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World Journal of Gastroenterology 2002年第1期8卷 31-35页

作者： Zhong-Ying Shen Jian Shen Qiao-Shan Li Department of pathology,medical college of shantou university,shantou 515031,Guandong province,china Cai-Yun Chen Central Lab.medical college of shantou university Jiong-Yu Chen Central Lab.of Tumor Hospital,medical college of shantou university Yi Zeng Institute of Virology,Chinese Academy of preventive Medicine,Beijing 100052,china Department of pathology Medical College of Shantou University 22 Xinglin Road Shantou 515031 Guandong Province China. zhongyingshen@ ***

AIM:To demonstrate that mitochondrial morphological andfunctional changes are an important intermediate link in thecourse of apoptosis in esophageal carcinoma calls inducedby As_2O_***:The esophageal carcinoma call line SHEEC1,established in our laboratory,was cultured in 199 growthmedium,supplemented with 100mL·L^(-1)calf serum and3 mol·L^(-1)As_2O_3(the same below).After 2,4,6,12,24 hof drug adding,the SHEECl calls were collected for light-and electron-microscopic *** mitochondriawere labeled by rhodamine fluorescence probe and thefluorescence intensity of the mitochondria was measured byflow cytorneter and cytofluorimetric ***,themitochondrial transmembrane potential(MTp,ΔΨm)change was also ***:The mitochondrial morphological change afteradding As_2O_3 could be divided into three *** theearly-stage(2-6h)after adding As203,an adaptiveproliferation of mitochondria appeared;in the mid-stage(6-12 h)a degenerative change was observed;and in the late-stage(12-24 h)the mitochondria swelled with outermembrane broken down and then calls death with apoptoticchanges of *** functional change of themitochondria indicated by fluorescent intensity,whichreflected the MTp status of mitochondria,was in accordancewith morphological change of the *** intensity increased at early-stage,decUned inmid-stage and decreased to the lowest in the late-stage.24h after As_2O_3 adding,the cell nucleus showed typicalapoptotic ***:Under the inducement of As_2O_3,the earlyapoptotic changes of SHEEC1 cells ware the apparentmorphological and functional changes of mitochondria,afterwards the nucleus changes *** is consideredthat changes of mitochondria are an important intermediatelink in the course of apoptosis of esophageal carcinoma cells induced by As_2O_3.

关键词：食道癌癌细胞凋亡三氧化砷形态学改变功能性改变纯粒体

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胎儿窘迫的诊断指标及其相关因素分析

实用妇产科杂志 2003年第3期19卷 159-160页

作者：郑楚銮汕头大学医学院第一附属医院广东汕头515041

目的 :探讨胎儿窘迫的诊断指标及其相关因素。方法 :分析 2 33例以胎儿窘迫为指征行剖宫产病例 ,按胎儿窘迫的不同诊断指标进行分组比较 ,统计其新生儿窒息率及胎儿窘迫相关因素百分率 ,并统计各胎儿窘迫相关因素的百分比。结果 :①随... 详细信息

目的 :探讨胎儿窘迫的诊断指标及其相关因素。方法 :分析 2 33例以胎儿窘迫为指征行剖宫产病例 ,按胎儿窘迫的不同诊断指标进行分组比较 ,统计其新生儿窒息率及胎儿窘迫相关因素百分率 ,并统计各胎儿窘迫相关因素的百分比。结果 :①随着胎儿窘迫诊断指标项目的增多 ,发现检出的新生儿窒息率和胎儿窘迫相关因素百分率也相应升高。②胎儿窘迫的主要相关因素为脐带因素、羊水过少、胎盘因素等。结论 :①胎儿窘迫应采取多指标来诊断。②对于有胎儿窘迫相关因素者 ,应密切监护、积极处理、适时行剖宫产术。

关键词：胎儿窘迫诊断指标新生儿窒息脐带因素羊水过少胎盘因素

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Atomic Force Microscopy-based Cell Nanostructure for Ligand-conjugated Quantum Dot Endocytosis

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 646-652页

作者： Yun-Long pAN~1* Ji-Ye CAI~2 Li QIN~3 Hao WANG~4 1 The First Affiliated Hospital of Jinan university,Guangzhou 510632,china 2 college of Life Science and Technology,Jinan university,Guangzhou 510632,china 3 Department of Histology and Embryology,medical college of Jinan university,Guangzhou 510632,china 4 Information and Optoelectronic Science and Engineering.South china Normal university,Guangzhou 510631,china The First Affiliated Hospital of Jinan universityGuangzhou 510632 China college of Life Science and Technology Jinan UniversityGuangzhou 510632 China Department of Histology and Embryology Medical College of Jinan UniversityGuangzhou 510632 China School for Information and Optoelectronic Science and Engineering South China Normal UniversityGuangzhou 510631 China

While it has been well demonstrated that quantum dots (QDs) play an important role inbiological labeling both in vitro and in vivo,there is no report describing the cellular nanostructure basis ofreceptor-mediated ***,nanostructure evolution responses to the endocytosis of transferrin(Tf)-conjugated QDs were characterized by atomic force microscopy (AFM).AFM-based nanostructureanalysis demonstrated that the Tf-conjugated QDs were specifically and tightly bound to the cell receptorsand the nanostructure evolution is highly correlated with the cell membrane receptor-mediated ***,confocal microscopic and flow cytometry results have demonstrated the specificity anddynamic property of Tf-QD binding and *** found that the internalization of Tf-QD is linearlyrelated to ***,while the nanoparticles on the cell membrane increased,the endocytosis was stillvery active,suggesting that QD nanoparticles did not interfere sterically with the binding and function ***,ligand-conjugated QDs are potentially useful in biological labeling of cells at a nanometerscale.

关键词： quantum dot endocytosis nanostructure atomic force microscopy

Voltage-dependence of intracellular local Ca2+ signal

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Voltage-dependence of intracellular local Ca2+ signal

Treatment with Vector-expressed Small Hairpin rNAs against Ki67 rNA-induced Cell Growth Inhibition and Apoptosis in Human renal Carcinoma Cells

中南地区第六届生理学学术会议

作者： Shen Jian-Xin Han Tai-Zhen (Department of physiology, shantou university medical college, shantou 515041, china Department of physiology, medical School of Xi'' an Jiaotong university, Xi'' an 710061, china)

＜正＞In quiescent cardiac cells,a Ca2 spark,which is generally assumed to be theelementary events of intracellular Ca2+ release,reflects a transient increase in localCa2+ due to the spontaneous activation of ryanodin...

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 254-261页

作者： Jun-Nian ZHENG~(1,3*) Ya-Feng SUN~(2#) Dong-Sheng pEI~2 Jun-Jie LIU~1 Teng-Xiang MA~3 rui-Fa HAN~3 Wang LI~1 Dian-Bao ZHENG~1 Jia-Cun CHEN~1 Xiao-Qing SUN~1 1 Laboratory of Urology,Affiliated Hospital of Xuzhou medical college,Xuzhou 221002,china 2 research Center for Biochemistry and Molecular Biology,Xuzhou medical college,Xuzhou 221002,china 3 Institute of Urology,medical university of Tianjin,Tianjin 300211,china Laboratory of Urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China Institute of Urology Medical University of TianjinTianjin 300211 China research Center for Biochemistry and Molecular Biology Xuzhou Medical CollegeXuzhou 221002 China

Short hairpin rNAs (shrNAs) transcribed *** polymerase Ⅲ promoters can triggersequence-selective gene silencing in mammalian *** virtue of their excellent function in knocking downexpression of cancer-associated genes,shrNAs could be used as new therapeutic agents for *** of Ki67 in renal cancer has been correlated to a more aggressive tumor phenotype,inhibitionof Ki67 protein expression by means of shrNAs seems to be a promising approach for the therapy of *** this study,we constructed an expression plasmid encoding shrNAs against the Ki67 gene,namedpSilencerKi67,and transfected it into human renal carcinoma *** pSilencerKi67 was shown to signifi-cantly knock down the expression of the Ki67 gene in human renal carcinoma cells,resulting in inhibitingproliferation and inducing apoptotic cell death that can be maintained for at least *** findings offer thepromise of using vector-based shrNAs against Ki67 in renal cancer gene therapy.

关键词： Ki67 gene shrNAs renal cell carcinoma proliferation apoptosis

Opening of the myocardial mitochondrial permeability transition pores in neonatal rats after asphyxia

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Inhibition of proliferation and Induction of Apoptosis in Human renal Carcinoma Cells by Anti-telomerase Small Interfering rNAs

中华医学会第十七次全国儿科学术大会

作者： XiaoYi Fang,NiYang Lin,YuGuang Li,JianQin Chen Department of Neonatology1 and Cardiology2,First Affiliated Hospital of shantou university medical college. 515041,shantou,china.

Objective Through detecting the opening of the myocardial mitochondrial permeability transition pores(MpTp) after intrauterine asphyxia in neonatal rats,to explore the mechanism of the myocardial hypoxic-ischemic and reperfusion injury caused by the opening of MpTp after asphyxia. Methods Cesarean sections were undertaken in female SD rats at the 21 day after pregnancy.

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 500-506页

作者： Jun-Nian ZHENG~(1,3#*)Ya-Feng SUN~(2#)Dong-Sheng pEI~2 Jun-Jie LIU~1 Jia-Cun CHEN~1 Wang LI~1 Xiao-Qing SUN~1 Qi-Duo SHI~3 rui-Fa HAN~3 Teng-Xiang MA~3 1 Laboratory of Urology,Affiliated Hospital of Xuzhou medical college,Xuzhou 221002,china 2 research Center for Biochemistry and Molecular Biology,Xuzhou medical college,Xuzhou 221002,china 3 Institute of Urology,Tianjin medical university,Tianjin 300070,china Laboratory of Urology Affiliated Hospital of Xuzhou Medical CollegeXuzhou 221002 China Institute of Urology Tianjin Medical UniversityTianjin 300070 China research Center for Biochemistry and Molecular Biology Xuzhou Medical CollegeXuzhou 221002 China

Telomerase is an attractive molecular target for cancer therapy because it is present in mostmalignant cells but is undetectable in most normal somatic *** telomerase consists of two subunits,an rNA component(hTr)and a human telomerase reverse transcriptase component(hTErT).Smallinterfering rNA(sirNA),one kind of rNA interferences,has been demonstrated to be an effective methodto inhibit target gene expression in human *** investigated the effects of sirNA targeting at both hTrand hTErT mrNA on the inhibition of telomerase activity in human renal carcinoma cells(HrCCs).Theproliferation and apoptosis of HrCCs were *** treatment of HrCCs using hTr and hTErTsirNAs resulted in significant decrease of hTr mrNA,hTErT mrNA and hTErT *** sirNA canalso inhibit the telomerase activity and the proliferation of ***,they can induce apoptotic celldeath in a dose-dependent *** these findings,we propose that the inhibition of telomerase activityusing sirNA targeting hTr and hTErT might be a rational approach in renal cancer therapy.

关键词： hTErT hTr telomerase sirNA renal cell carcinoma proliferation apoptosis

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核基质与细胞凋亡

生物化学与生物物理进展 2002年第4期29卷 523-526页

作者：余跃飞温博贵汕头大学医学院生物化学教研室汕头515031

阐述了凋亡过程中 ,核基质所发生的形态、生化变化及相关凋亡基因的表达 ,尤其是凋亡早期便出现核基质蛋白的降解 .核基质是细胞核最基本的组分 ,对维持细胞核形态结构和功能非常重要 ,其主要由核纤层 ,核内骨架及核孔复合体构成 ,在DN... 详细信息

阐述了凋亡过程中 ,核基质所发生的形态、生化变化及相关凋亡基因的表达 ,尤其是凋亡早期便出现核基质蛋白的降解 .核基质是细胞核最基本的组分 ,对维持细胞核形态结构和功能非常重要 ,其主要由核纤层 ,核内骨架及核孔复合体构成 ,在DNA复制、转录、rNA加工转运等事件中起支持作用 .多少年来 ,关于凋亡时细胞核形态及生化改变的分子机理一直未阐明 ,最近对核基质与细胞凋亡的研究取得了重大进展 .

关键词：核基质细胞凋亡核基质结合区凋亡基因

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