Morphological and functional changes of mitochondria in apoptotic esophageal carcinoma cells induced by arsenic trioxide

作     者：Zhong-Ying Shen Jian Shen Qiao-Shan Li Department of Pathology,Medical College of Shantou University,Shantou 515031,Guandong Province,China Cai-Yun Chen Central Lab.Medical College of Shantou University Jiong-Yu Chen Central Lab.of Tumor Hospital,Medical College of Shantou University Yi Zeng Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052,China 

作者机构：Department of Pathology Medical College of Shantou University 22 Xinglin Road Shantou 515031 Guandong Province China. zhongyingshen@ *** 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2002年第8卷第1期

页      面：31-35页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China No.39830380 

主　　题：食道癌 癌细胞凋亡 三氧化砷 形态学改变 功能性改变 纯粒体 

摘      要：AIM:To demonstrate that mitochondrial morphological andfunctional changes are an important intermediate link in thecourse of apoptosis in esophageal carcinoma calls inducedby As_2O_***:The esophageal carcinoma call line SHEEC1,established in our laboratory,was cultured in 199 growthmedium,supplemented with 100mL·L^(-1)calf serum and3 mol·L^(-1)As_2O_3(the same below).After 2,4,6,12,24 hof drug adding,the SHEECl calls were collected for light-and electron-microscopic *** mitochondriawere labeled by Rhodamine fluorescence probe and thefluorescence intensity of the mitochondria was measured byflow cytorneter and cytofluorimetric ***,themitochondrial transmembrane potential(MTP,ΔΨm)change was also ***:The mitochondrial morphological change afteradding As_2O_3 could be divided into three *** theearly-stage(2-6h)after adding As203,an adaptiveproliferation of mitochondria appeared;in the mid-stage(6-12 h)a degenerative change was observed;and in the late-stage(12-24 h)the mitochondria swelled with outermembrane broken down and then calls death with apoptoticchanges of *** functional change of themitochondria indicated by fluorescent intensity,whichreflected the MTP status of mitochondria,was in accordancewith morphological change of the *** intensity increased at early-stage,decUned inmid-stage and decreased to the lowest in the late-stage.24h after As_2O_3 adding,the cell nucleus showed typicalapoptotic ***:Under the inducement of As_2O_3,the earlyapoptotic changes of SHEEC1 cells ware the apparentmorphological and functional changes of mitochondria,afterwards the nucleus changes *** is consideredthat changes of mitochondria are an important intermediatelink in the course of apoptosis of esophageal carcinoma cells induced by As_2O_3.