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高效液相色谱法测定金水宝胶囊中腺苷及腺嘌呤的含量

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中国新药与临床杂志 2011年第10期30卷 786-788页

作者：贾增永王玉宿迁市中医院药剂科江苏宿迁223800 宿迁市药品检验所江苏宿迁223800

目的建立测定金水宝胶囊中腺苷及腺嘌呤含量的高压液相色谱方法。方法采用0.5%磷酸超声提取制备金水宝胶囊样品溶液,色谱柱为Venusil-XBP-C18(250 mm×4.6 mm,5μm);流动相采用[0.01 mol.L-1 NaH2PO4∶0.01 mol.L-1 Na2HPO4(1∶2.2,V/V)... 详细信息

目的建立测定金水宝胶囊中腺苷及腺嘌呤含量的高压液相色谱方法。方法采用0.5%磷酸超声提取制备金水宝胶囊样品溶液,色谱柱为Venusil-XBP-C18(250 mm×4.6 mm,5μm);流动相采用[0.01 mol.L-1 NaH2PO4∶0.01 mol.L-1 Na2HPO4(1∶2.2,V/V)]∶甲醇(17∶3,V/V),流速0.6 ***-1,进样量10μL,柱温25℃,检测波长260 nm,运行时间18 min。运行时用流动相∶甲醇(85∶15,V/V)梯度洗脱。结果腺苷及腺嘌呤分别在0.62～157.07 mg.L-1和0.23～58.29 mg.L-1内线性关系良好;两者的平均回收率分别为(97.40±4.28)%和(99.10±5.03)%;3种批号的金水宝胶囊样品中腺苷的含量分别为(0.324±0.017)%、(0.318±0.025)%和(0.331±0.019)%;腺嘌呤的含量分别为(0.089±0.009)%、(0.086±0.012)%和(0.093±0.007)%。结论高效液相色谱法测定金水宝胶囊中腺苷及腺嘌呤的含量操作简便、结果准确可靠、灵敏度高、重复性好,能作为有效控制金水宝胶囊质量的测定方法之一。

关键词：色谱法高压液相腺苷腺嘌呤金水宝胶囊

The Role of Sciencein Codex Standards

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Biomedical and Environmental Sciences 2001年第1期14卷 145-148页

作者： CHEN JUN SHI institute of Nutrition and food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, china 中国预防医学科学院营养与食品卫生所北京 100050

The key principle in the development of Codex standards is to ensure that the decision making process is based on sound science. The approval for the use of food additives and other chemicals in foods should be made only when adequate scientific data is available. The same principle applies to the development of maximum tolerable (or residue) levels for contaminants in foods. Using the General Standards for Contaminants and Toxins in foods as an example, the following criteria are considered, when developing recommendations and standards: Toxicological information; Analytical data; Intake data; Fair trade considerations; Technological considerations; and Risk assessment and risk management considerations. The Codex Alimentarius Commission has repeatedly emphasized the use of risk analysis approach, in particular the use of risk assessment in conducting the safety evaluation of food additives, agricultural and veterinary chemicals and environmental and industrial contaminants in foods. In this respect, the well known efforts of the FAO/WHO Joint Expert Committee on food Additives (JECFA) and the FAO/WHO Joint Meeting on Pesticide Residues (JMPR) provide the much needed information and recommendations for the risk assessment of specific chemicals. The risk assessment approach is also used in the safety evaluation of foods derived from modern biotechnology as well as in the quantitative assessment of microbiological risks in foods.

关键词： JECFA The Role of Sciencein Codex Standards

Highly efficient transformation of intact yeast-like conidium cells of Tremella fuciformis by electroporation

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Science china(Life Sciences) 2008年第10期51卷 932-940页

作者： GUO LiQiong1,2, LIU Yong1, ZHAO ShuXian1, LIU ErXian1 & LIN JunFang1,2 1 College of food Sciences, South china Agricultural University, Guangzhou 510640, china 2 institute of Biomass Research, South china Agricultural University, Guangzhou 510640, china 1. College of food Sciences South China Agricultural University Guangzhou 510640 China 2. institute of Biomass Research South China Agricultural University Guangzhou 510640 China

Tremella fuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. So it is a good recipient cell for exogenous gene expression. In this study, two expression vectors pGlg-gfp containing gpd-Gl promoter and gfp gene and pGlg-hph containing gpd-Gl promoter and hph gene were constructed. The lowest sensitive concentration of hygromycin for the blastospore was determined on three types of media. Our ex- periments showed that the lowest sensitive concentration of hygromycin for the blastospore was 5 μg/mL on MA medium. The intact blastospores were transformed with the expression vector pGlg-hph by electroporation. The putative transformants were obtained by the MA selective medium. Experi- mental results showed that the most effective parameters for the electroporation of intact blastospores were obtained by using STM buffer, 1.0×108 cells/mL of blastospores, 200 μL in transformation volume, 6 μg plasmid, 2.0 kV/cm of electric pulse voltage, stillness culturing on MB liquid medium for 48 h after electroporation. In these transformation conditions, the efficiency reached 277 colonies/μg DNA. Co-transformation of plasmid pGlg-gfp and pGlg-hph with ratio of 1:1 was performed by electroporation with the optimal parameters. The putative co-transformants were obtained by the MA selective medium. Eight randomly selected colonies from the vast putative co-transformants were analyzed by PCR de- tection and Southern blotting. The experiments showed that the gfp was integrated into the genomes of three transformants. The co-transformation efficiency was 37.5%. Green fluorescence was observed under laser scanning confocal microscope in these gfp positive transformants. This indicates that the exogenous gfp can be expressed effectively in the yeast-like conidia of T. fuciformis.

关键词： Tremella fuciformis electroporation yeast-like conidia intact cells gene expression hph gene gfp gene

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高血压病中医分型的代谢组学研究(英文)

高血压病中医分型的代谢组学研究(英文)

Expression and function of heregulin-α and its receptors in the mouse mammary gland

中国药理学会临床药理学专业委员会会议暨第十次全国临床药理学学术会议

作者： LU Yi-hong~(1,3) HAO Hai-ping~1 WANG Guang-ji~1 CHEN Hu-xiao~2 ZHU Xuan-xuan~2 XIANG Bing-ren~1 HUANG Qing~1 A Ji-Y~1 1 Key Laboratory of drug Metabolism and Pharmacokinetics,china Pharmaceutical University,nanjing 210009,jiangsu,china 2 Jinagsu Provincial Traditional Chinese Medicinal Hospital,nanjing 210009,jiangsu,china 3 jiangsu institute for drug control,nanjing 210008,jiangsu,china

目的：将传统中医辨证方法同现代系统生物学理论相结合,探讨原发性高血压辨证分型与基于 GC/MS 的血清代谢组学的关系。方法：原发性高血压辨证分为肝火亢盛、痰湿雍盛及阴虚阳亢三型。应用 GC/MS 测定健康人及原发性高血压病人血清内... 详细信息

目的：将传统中医辨证方法同现代系统生物学理论相结合,探讨原发性高血压辨证分型与基于 GC/MS 的血清代谢组学的关系。方法：原发性高血压辨证分为肝火亢盛、痰湿雍盛及阴虚阳亢三型。应用 GC/MS 测定健康人及原发性高血压病人血清内源性代谢物,并用主成分分析(PCA)、偏最小乘方分析(PLS-DA)和马氏距离(MD)分析他们的代谢谱。结果：PCA 和 PLS-DA 分析的结果表明：健康人与高血压病人血清代谢谱有明显差异,能够被区分开, 但 PCA 和 PLS-DA 不能将中医高血压的三型完全分开。利用 MD 不仅可以清晰地区分上述三种类型的高血压,同时还显示高血压的发展过程。结论：基于 GC/MS 和模式识别的代谢组学在揭示传统中医理论本质上有着广泛的应用前景。

关键词：代谢组学中医证型高血压 GC/MS

来源： cnki会议评论

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Science china(Life Sciences) 2010年第8期53卷 1015-1024页

作者： GENG LiJing1 & LI QingZhang2 1institute of food Science and Engineering, Liaoning Medical University, Jinzhou 121000, china 2Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin 150030, china 1. institute of food Science and Engineering Liaoning Medical University Jinzhou 121000 China2. Key Laboratory of Dairy Science of Ministry of Education Northeast Agricultural University Harbin 150030 China

Heregulin-α (HRGα) is a cytokine secreted by the mammary mesenchyme, adjacent to lobuloalveolar structures. To understand the role of HRGα and its receptors in mammary glands, and the underlying mechanisms, we performed this study to determine the expression and localization of HRGα and its receptors ErbB2 and ErbB3. We also determined the role of HRGα in the development of mammary glands, β-casein expression and secretion, Rab3A protein expression and the phosphorylation of HRGα signaling molecules using confocal laser scanning microscopy, tissue culture, capillary electrophoresis, Western blotting and enzyme-linked immunosorbent assays. We found that a peak was on pregnancy day 15. Changes of ErbB2 and ErbB3 expression were positively and linearly correlated with HRGα, indicating that HRGα positively regulates ErbB2 and ErbB3 expression. During pregnancy, HRGα enhanced the phosphorylation of STAT5, p42/p44, p38, PKC and Rab3A protein expression, stimulated the proliferation and differentiation of the ductal epithelial cells of mammary glands, and increased and maintained the expression and secretion of β-casein. During lactation, HRGα enhanced the phosphorylation of STAT5 and p38, inhibited the phosphorylation of PKC and Rab3A protein expression, maintained the morphology of the mammary glands and increased the secretion of lactoprotein to reduce the expression of β-casein in mammary epithelial cells. During involution, HRGα induced the phosphorylation of STAT3 and Rab3A protein expression, and inhibited the phosphorylation of PKC to stimulate the degeneration of mammary epithelial cells. It also inhibited the secretion of β-casein, resulting in increased levels of β-casein in mammary epithelial cells.

关键词： mammary gland heregulin-α ErbB2 ErbB3 signal transduction

反相高效液相色谱法测定复方氨基酸注射液中的乙酰半胱氨酸和乙酰酪氨酸

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中国生化药物杂志 2003年第4期24卷 194-195页

作者：吴小曼纪宇江苏省药品检验所江苏南京210008

目的建立一种等度洗脱反相高效液相色谱法测定复方氨基酸注射液中的N 乙酰 L 半胱氨酸和N 乙酰 L 酪氨酸。方法KromasilC1 8柱 (2 0 0mm× 4 .6mm ,5 μm) ,流动相为 2 0mmol L磷酸二氢钠溶液 (用 5 0 %H3PO4 溶液调pH 2 .5 ) 乙腈 ... 详细信息

目的建立一种等度洗脱反相高效液相色谱法测定复方氨基酸注射液中的N 乙酰 L 半胱氨酸和N 乙酰 L 酪氨酸。方法KromasilC1 8柱 (2 0 0mm× 4 .6mm ,5 μm) ,流动相为 2 0mmol L磷酸二氢钠溶液 (用 5 0 %H3PO4 溶液调pH 2 .5 ) 乙腈 (94∶6 ) ,流速为 1.0ml min ,进样量 10 μl,检测波长 2 10nm。结果N 乙酰 L 半胱氨酸和N 乙酰 L 酪氨酸均在 6 0～ 14 0 μg ml浓度范围内呈良好的线性关系 (r=0 .9999) ,平均回收率分别为 10 2 .2 %、99.9% ,RSD(n =9)分别为 0 .6 %、0 .5 %。结论此法简便实用。

关键词：反相高效液相色谱法 N-乙酰-L-半胱氨酸 N-乙酰-L-酪氨酸含量测定

Effects of Red Palm Oil on Serum Lipids and Plasma Carotenoids Level in Chinese Male Adults

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Biomedical and Environmental Sciences 2003年第4期16卷 348-354页

作者： JIAN ZHANG , CHUN-RONG WANG, AN-NA XUE, AND KE-YOU GEinstitute of Nutrition and food Safety, Chinese Center for Disease control and Prevention, Beijing 100050, china institute of Nutrition and food Safety Chinese Center for Disease Control and Prevention Beijing 100050 Chin

Objective Effects of red palm oil on major plasma carotenoids, tocopherol, retinol and serum lipids were evaluated when used in Chinese diet. Methods Red palm oil group (RPO) composed of 20 male subjects(aged 18-32) and soybean oil group (SBO) composed of 22 male subjects (aged 18-32). Dietary fat provided about 28% of total calories, and the test oil accounted for about 60% of total dietary fat. In the 3 weeks of pretest period, diets were prepared with soybean oil, and then in the next 6 weeks subjects in each group consumed the diet prepared by test oil. Results Plasma α-carotene, β-carotene and lycopene concentration of RPO group significantly increased at the time of interim (21 days) and of the end (42 days) (P2 days) in this study. Though Chinese plasma retinol level was relatively low when compared with that of Westerners, red palm oil diet showed no significant effect on adult Chinese plasma retinol level. Serum concentration of total cholesterol, triglyceride, high density lipoprotein cholesterol, apolipoprotein AI and apolipoprotein B of all subjects showed no significant changes in RPO group during the study. Conclusions The data in our study suggest that red palm oil is a good source of carotenoids and vitamin E when used in Chinese diet preparation, and it can significantly increase plasma concentration of a-carotene, α-carotene, lycopene and β-tocopherol.

关键词： Red palm oil Retinal α-carotene β-carotene α-tocopherol Serum cholesterol Serum triglyeride Apolipoprotein AI Apolipoprotein B

Practice and Thought on Developing Hybrid Rice for Super High Yield by Exploiting Inter-subspecific Heterosis

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Rice science 2005年第1期12卷 1-6页

作者： LuChuan-gen ZouJiang-shi instituteoffoodCrops JiangsuAcademyofAgriculturalSciencesNanjing210014China

Since the breakthrough of grain yield owing to the development of dwarf rice and three-line system hybrid rice, rice breeding for high yield hardly had showed significant progress in the next successive two decades. It was considered that utilizing heterosis between subspecific varieties (Oryza sativa L.) would be an effective approach to increase yield further. During 1987-1993, an indica-japonica hybrid Yayou 2 yielded as high as 10.5 t/ha; however, it failed to be commercialized because of seed purity problem due to non-uniform emasculation by chemical agent in seed production, and sensitivity of seed setting in Ft plants to environmental conditions. In the past decade, two inter-subspeific hybrids, Liangyoupeijiu (Peiai 64S/9311, javanica/indica) and Liangyou E32 (Peiai 64S/E32, javanica/ laponica); both of them exhibited grain yield higher than 10.5 t/ha, and were widely judged as the pioneers of super hybrid rice. Liangyoupeijiu has been successfully popularized over 4 million hectare in wide climatic areas, while Liangyou E32 made a yield record and offered a model of plant ideotype for super hybrid rice. It was considered that in combination with plant ideotype, active physiological functions, and wide-range adaptability to ecological conditions, exploitation of indica-japonica heterosis would be the key approach for super hybrid rice breeding.

关键词： rice breeding intersubspecific hybrid plant type two-line approach

儿科人群药物临床试验技术指导原则(征求意见稿)

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中国临床药理学杂志 2015年第16期31卷 1696-1700页

作者： Center for drug Evaluation,china food and drug Administration 国家食品药品监督管理总局药品审评中心

临床上儿科超说明书用药极为普遍,如何使儿童安全、有效用药是药物研发领域的热点,但是规范与推动儿科药物临床试验是一项全球性的难题。儿科人群的生理机能与成人相比具有其特殊性,因此,儿科人群药物临床试验相比于成人临床试验面临更... 详细信息

临床上儿科超说明书用药极为普遍,如何使儿童安全、有效用药是药物研发领域的热点,但是规范与推动儿科药物临床试验是一项全球性的难题。儿科人群的生理机能与成人相比具有其特殊性,因此,儿科人群药物临床试验相比于成人临床试验面临更全面和更完善的方案设计和可操作要求。近些年来,美国和欧盟已陆续颁布了针对儿科人群药物临床试验的指导性建议,加强对其试验过程的规范力度;但是在我国,目前颁布的与药物临床试验相关的法律法规或指南要求中,尚并未专门针对儿科人群药物临床试验提出具体的指导建议。2015年8月4日,国家食品药品监督管理总局药品审评中心发布了《儿科人群药物临床试验技术指导原则》征求意见稿。这是我国药品监管部门首次专门针对儿科人群药物临床试验提出的指导性建议。该指导原则制订的目的是进一步规范我国儿科人群药物临床试验设计,提高试验质量,使试验数据能充分地支持儿科药物上市,同时避免不必要的重复试验。该指导原则为国内儿科药物研发企业及儿科药物临床试验机构提供了可参考的临床试验基本原则和操作指南。

关键词：儿科药物临床试验技术指导人群