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第九届全国药物和化学异物代谢学术会议

作者： A Ji-ye~1,WANG Guang-ji~(1*),HAO Hai-ping~1,HUANG Qing~2,LU Yi-hong~2,YAN Bei~1, ZHA Wei-bin~1,GU Sheng-hua~1,ZHANG Ying~1,LIU Lin-sheng~1,CAO Bei~1,SHI Jian~1,LI Meng-jie~1, WANG Xin-wen~1,ZHAO Chun-yan~1,ZHANG Mei~2,FAN Xia-lei~2 1 Key Laboratory of drug Metabolism and Pharmacokinetics,china Pharmaceutical University,nanjing 210009, jiangsu,china 2 jiangsu institute for food and drug control,nanjing 210008,jiangsu,china

AIM:Essential hypertension is inherently associated with metabolic disorders,and its pathogenesis remains poorly *** of the metabolic perturbation of hypertension and the regulation of this perturbed metabolism using therapeutic agents can extend our insight into the pathogenesis of this ***:We used gas chromatography mass spectrometry and principal component analysis to profile the metabolic phenotype of hypertension in spontaneously hypertensive rats(SHR) and studied the regulatory effect of total ginsenosides(TG) and four typical antihypertensive agents on in vivo ***:The five agents yielded different hypotensive effects and distinct regulation patterns on the perturbed metabolism that occurs in ***,amlodipine,and terazosin efficiently depressed systolic blood pressure(SP) but had a negligible regulatory effect on the perturbed metabolism of SHR,even after the remission of hypertension for 8 *** finding suggests that hypertension may not be the cause of the metabolism perturbation observed in these *** contrast,TG gradually and markedly regulated the perturbed metabolism, and led to a mild but sustained depression of SP up to 2 weeks after the cessation of ***, TG downregulated free fatty acids(FFA) toward normal levels;the elevation of FFA correlates with blood pressure,in both animals and ***:These results suggest that TG may act as a special antihypertensive agent that regulates the perturbed metabolism of hypertension.

关键词： metabonomics hypertension total ginsenosides spontaneously hypertensive rats free fatty acids

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Fingerprint analysis of placenta polypeptide injection by high performance liquid chromatography

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Journal of Pharmaceutical Analysis 2012年第1期2卷 71-75页

作者： Li Huanga, Xiao-Man Wub, Yu Jib, Yu Wanga,bachina Pharmaceutical University, nanjing 210009, china bjiangsu institute for food and drug control, nanjing 210008, china china Pharmaceutical University Nanjing 210009 China. jiangsu institute for food and drug control Nanjing 210008 China.

Objective: To develop the representative fingerprint for the quality control of placenta polypeptide injection. Methods: The chromatographic separation was performed using a Phenomenex Gemini C18 column (250 mm 4.6 mm, 5 mm) maintained at 30 1C. 0.1% aqueous trifiuoroacetic acid (Solvent A) and acetonitrile contained 0.1% TFA (Solvent B) were used as mobile phase with a gradient elution. Detection wavelength was 280 nm with the sample injection volume of 50 mL; the fiow rate was 1.0 mL/min. The fingerprints of different samples were investigated by similarity analysis. Results: Nine peaks were identified as the characteristic common peaks. The similarities of the fingerprints of the 10 batches of samples were above 0.992. Conclusion: This method showed high precision and good repeatability, and provided the basis for the improvement of the quality control of placenta polypeptide injection.

关键词： Placenta polypeptide injection Fingerprint Similarity analysis High performance liquid chromatography

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Anticancer effect of two diterpenoid compounds isolated from Annona glabra Linn

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Acta Pharmacologica Sinica 2004年第7期25卷 91-96页

作者： Yong-hong ZHANG2, Hai-yan PENG3, Guo-hao XIA3, Ming-yan WANG3, Ying HAN4 First Clinical College 3Basic Medicine College, nanjing University of TraditionalChinese Medicine, nanjing 210029 4jiangsu institute of Materia Medica, nanjing 210009, china First Clinical College Basic Medicine College Nanjing University of Traditional Chinese Medicine jiangsu institute of Materia Medica

AIM: To study the inhibitory effect of two diterpenoid compounds isolated from Annona glabra Linn (Cunabic acid and ent-kauran-19-al-17-oic acid) on the proliferation of Human Liver Cancer (HLC) cell line SMMC-7721 and its mechanism. METHODS: Inhibition of cell proliferation was measured by MTT assay. The morphological changes of SMMC-7721 cells were observed under inverted phase-contrast microscope, fluorescent microscope, transmission electron microscope (TEM), and scanning electron microscope (SEM). Flow cytometer (FCM) was used to calculate the cell apoptotic rate, and immunohistochemical staining was used to observe the regulation of gene expression. RESULTS: The proliferation of SMMC-7721 cells was obviously inhibited after being treated with Cunabic acid at the concentration >5 μmol/L and ent-kauran-19-al-17-oic acid >10 μmol/L. The biggest inhibitory effect was 81.05 % when treated with Cunabic acid at the concentration of 25 μmol/L. The effect had a linear relationship with concentration. The result indicated that drug-treated cells exhibit typical morphological changes of apoptosis, including condensed chromatin and a reduction in volume. Sub-G0/G1 peak was found by FCM analysis and the cell cycle was arrested at G0/G1 stage. The apoptotic rates of the cells treated by Cunabic acid and ent-kauran-19-al-17-oic acid were 43.31 % and 24.95 %, respectively. It was visualized by immunohis- tochemical staining that the drugs down-regulated the gene expression of bcl-2 gene and up-regulated that of bax gene. CONCLUSION: The two diterpenoid compounds isolated from Annona glabra Linn, Cunabic acid and ent- kauran-19-al-17-oic acid can obviously inhibit the proliferation of HLC cell line SMMC-7721. The mechanism is correlated with the induction of cell apoptosis by down-regulating the gene expression of bcl-2 gene and up- regulating that of bax gene.

关键词： Annona glabra Linn diterpenes hepatocellular carcinoma apoptosis gene expression

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Principal aspects of the Ordovician biotic radiation

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Science china Earth Sciences 2010年第3期53卷 382-394页

作者： ZHANG YuanDong,ZHAN RenBin,FAN JunXuan,CHENG JunFeng & LIU Xiao LPS, nanjing institute of Geology and Palaeontology, Chinese Academy of Sciences, nanjing 210008, china 1. LPS Nanjing Institute of Geology and Palaeontology Chinese Academy of Sciences Nanjing 210008 China

The Ordovician radiation is among the major bioevents in earth history that have drawn great attention from geologists all over the world, and there have been hundreds of publications treating various aspects of this radiation. The study of the event involves a broad spectrum of research fields and concerns many scientific questions. Many opinions and hypotheses have been proposed on the triggering and controlling factors of the radiation. Herein the authors critically review the study of the Ordovician radiation and dissect four principal aspects by synthesizing the studies over the past years: (1) relationships of the radiation to fossil clades, ecotypes, and environments, such as water depth, latitude, and climate; (2) the ecological structure and food webs of Ordovician marine organisms; (3) fossil records and true biodiversities; and (4) likely triggering and controlling factors of the Ordovician radiation. The authors also recommend approaches to furthering studies of these aspects.

关键词： Ordovician biotic radiation guild food web fossil record mechanism

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非理想状态下的塔板理论模型

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色谱 2002年第1期20卷 30-33页

作者：王少坤夏芸汪圣利江苏省药品检验所江苏南京210008 江苏省疾病控制中心江苏南京210009 南京大学数学系江苏南京210008

在色谱过程中 ,将流动相看成是由许多连续的塔板组成 ,每一塔板的高度与固定相塔板的高度相同。初始浓度的溶质被认为全部集中在流动相的第一塔板中 ;溶质在流动相和固定相之间动态分布。由于色谱动力学因素的影响 ,当流动相流过一个塔... 详细信息

在色谱过程中 ,将流动相看成是由许多连续的塔板组成 ,每一塔板的高度与固定相塔板的高度相同。初始浓度的溶质被认为全部集中在流动相的第一塔板中 ;溶质在流动相和固定相之间动态分布。由于色谱动力学因素的影响 ,当流动相流过一个塔板距离时 ,溶质不能够迅速地从固定相释放到流动相中 ,因此溶质在流动相和固定相中的分布浓度受到两个因素即反映色谱系统热力学性质的分配系数P和反映色谱系统动力学性质的释放概率因子α的影响。这一过程被认为是非理想状态下的色谱过程。采用统计学的观点 ,综合分析了非理想状态下P和α对色谱分布的影响 ;利用计算机技术模拟了色谱分布曲线 ,最终建立了一种非理想状态下的塔板理论分布模型 ,推导出了非理想状态下色谱分布方程 ,提出了释放概率因子和分配系数对色谱结果共同作用的模式。结果表明色谱释放概率因子可以作为衡量色谱系统优劣的一个指数。

关键词：非理想状态塔板理论模型色谱释放概率因子

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Metabonomic phenotype and identification of “heart blood stasis obstruction pattern” and “qi and yin deficiency pattern” of myocardial ischemia rat models

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Science china(Life Sciences) 2009年第11期52卷 1081-1090页

作者： YAN Bei1, A JiYe1, HAO HaiPing1, WANG GuangJi1, ZHU XuanXuan2, 3, ZHA WeiBin1, LIU LinSheng1, GUAN EnZe3, ZHANG Ying1, GU ShengHua1, HUANG Qing1 & ZHENG YuanTing1 1Key Laboratory of drug Metabolism and Pharmacokinetics, china Pharmaceutical University, nanjing 210009, china 2Pharmacological Laboratory of Clinical Research institute, jiangsu Provincial Hospital of Traditional Chinese Medicine, nanjing 210029, china 3College of Pharmacy, nanjing University of Traditional Chinese Medicine, nanjing 210029, china 1. Key Laboratory of drug Metabolism and Pharmacokinetics China Pharmaceutical University Nanjing 210009 China 2. Pharmacological Laboratory of Clinical Research institute Jiangsu Provincial Hospital of Traditional Chinese Medicine Nanjing 210029 China 3. College of Pharmacy Nanjing University of Traditional Chinese Medicine Nanjing 210029 China

The traditional Chinese medicine concepts of "Xinxueyuzuzheng (heart blood stasis obstruction pattern)" and "Qiyinliangxuzheng (qi and yin deficiency pattern)" for myocardial ischemia rat models were constructed in the present study. Endogenous metabolites in rat plasma were analyzed using the GC/TOF-MS-based metabonomic method. Significant metabolic differences were observed between the control and two model groups, and the three groups were distinguished clearly by pattern recognition. Compared with those of the control, the levels of hydroxyproline, threonic acid, glutamine and citric acid were strikingly up or down-regulated in model rats. The metabolites contributing most to the classification between the two "pattern" rats were identified, such as valine, serine, threonine, ornithine, hydroxyproline, lysine, 2-hydroxybutanoic acid, 3-hydroxybutanoic acid, galactofuranose and inositol. These compounds were indicated as the potential biomarkers. The results suggested that the two "patterns" are involved in dysfunction in oxidative stress, energy metabolism and amino acid metabolism. These findings also provided the substantial foundation for exploring the scientific connotation of these two "Zhengxing (pattern types)" of myocardial ischemia, and "Bianzheng (pattern identification)".

关键词： Metabonomics GC/TOF-MS heart blood stasis obstruction pattern qi and yin deficiency pattern myocardial ischemia rat model

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Safety Evaluation of Irradiated foods in china: A Condensed Report

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Biomedical and Environmental Sciences 1989年第1期2卷 1-6页

作者： YIN DAI institute of food Safety control and Inspection, Ministry of Public Health, Beijing, china institute of food Safety control and Inspection Ministry of Public Health Beijing China.

Eight trials, with 439 human volunteers who consumed irradiated foods including rice, potatoes, mushrooms, peanuts, and Chinese sausages, as well as diets composed of multiple irradiated foods (irradiated at dosages of 0.2 to 8 kGy) that accounted for 60-66％ of the entire diet, were carried out for 2-3 months according to a unified protocol. No adverse effects on body weight, blood pressure, ECG, hematology, blood enzyme activities, serum lipids, or blood or urine 17-hydroxycortisol contents and no chromosomal aberration of peripheral blood lymphocytes were found. It is especially worthwhile to note that there was no change in the polyploidy after consumption of irradiated diets. On the basis of these results and a comprehensive analysis of the physical and chemical characteristics of irradiated foods, temporary hygienic standards for irradiated rice, potatoes, onions, garlic, Chinese sausages, peanuts, and mushrooms were promulgated by the Chinese Ministry of Public Health. 1989 Academic Press, Inc.

关键词： Safety Evaluation of Irradiated foods in china In A Condensed Report

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Occurrence of nitrification-denitrification and gaseous nitrogen loss process in flooded rice soil

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Progress in Natural Science:Materials International 2007年第1期17卷 6-10页

作者： NI Wuzhong, LI Jianping and ZHU Zhaoliang (College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310029, china College of Biosystems Engineering and food Science, Zhejiang University, Hangzhou 310029, china institute of Soil Science, Chinese Academy of Sciences, nanjing 210008, china) [a]Gllege of Environmental and Resmirce Sciences Zheiang University Hangzhou China [b]Gllege of Biosystems Engineering and food Science Zheiang University Hangzhou China [c]institute of Soil Science Chinese Academy of Sciences Naning China

Flooding in wetland rice fields soon after transplanting results in displacement of soil air (including O2). Thus any dissolved O2 in the pore water is consumed out by microbial respiration in a short period. Supply of O2 to the flooded rice soil is by diffusion of O2 through the standing floodwater and consumption at the soil-water interface, and by exudation of O2 by rice roots and subsequent diffusion of O2 into the rhizosphere. The greater potential consumption of O2 compared to the renewal rate results in the development of distinct soil layers: oxidized soil layers under soil-water interface and in the rhizosphere, and reduced soil layers or reduced bulk soil. Nitrification in oxidized soils and denitrification in reduced soils have been known. Currently, denitrification in oxidized soils, even in standing floodwater, has also been identified. In this article, we present a modified nitrification and denitrification occurring mechanism in flooded rice soil.

关键词： denitrification flooded rice soil gaseous nitrogen loss nitrification.

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Determination of 23 β_2-agonists and 5 β-blockers in animal muscle by high performance liquid chromatography-linear ion trap mass spectrometry

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Science china Chemistry 2010年第4期53卷 832-840页

作者： WU YongNing1,2, MIAO Hong1, FAN Sai1,2 & ZHAO YunFeng1,2 1 institute of Nutrition and food Safety, Chinese Centre for Disease control and Prevention, Beijing 100021, china 2 College of Veterinary Medicine, china Agricultural University, Beijing 100193, china 1. institute of Nutrition and food Safety Chinese Centre for Disease Control and Prevention Beijing 100021 China2. College of Veterinary Medicine China Agricultural University Beijing 100193 China

A high performance liquid chromatography-linear ion trap mass spectrometry method using isotope dilution technique has been developed for the simultaneous determination of 23 β2-agonists and 5 β-blockers in animal muscle tissues. Pork and chicken muscle samples were acid hydrolyzed and extracted with 5% trichloracetic acid in water, and then cleaned up using MCX solid phase extraction (SPE) cartridge. Methanol and 0.1% formic acid were used as mobile phases for gradient elution. A Waters AtlantisT3 column was used for separation. ESI positive ion scan mode was used with selective reaction monitoring. 9 β2-Agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 23 β2-agonists and 5 β-blockers were 5-200 μg/L, the coefficient of correlation was not less than 0.995, and the limit of detection for each compound in the muscle tissue was below 0.2 μg/kg. The recoveries of each compound in the spiked samples at three levels 5, 10, 20 μg/kg were in the range of 47.3%-123.7%, and the relative standard deviations were in the range of 3.2%-25.7%. The developed method is sensitive and specific for the determination of β2-agonists and β-blockers in pork and chicken muscle samples.

关键词： β2-agonists β-blockers muscle determination HPLC-ITMS

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Effects of "Products of Chicken Embryo" on Growth and Sexual Development in Rats

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Biomedical and Environmental Sciences 1999年第2期12卷 125-135页

作者： WU CHUAN-JIN AND DAI YIN(institute of food control and Inspection, Ministry of Health, Chinese Academy of Preventive Medicine, Beijing 100021, china) institute of food control and Inspection Ministry of Health Chinese Academy of Preventive Medicine Beijing China

Products of Chicken Embryo (PCE) such as Ji-Pei-Jing is a kind of food for Chinese children prepard from chicken embryo. Female rats on 21 days were administered with aqueous solutions of Ji-Pei-Jing (1. 2 %, 3 %, 12%, and 48%, respectively) by gavage up to their onsets of *** rats in the control group were treated with distilled water. However, Ji-Pei-Jing treatment exerted some effects on sexual mauration in the immature female rats. Essentially, the effects showed a dose-response tendency with an inverted 'U' *** age of vopnal opening for gnup treated with Ji-Pei-Jing was significantly earier than that to the control. Its uterus weight/b. w. ratio also significantly increased on day 30 and at the first estrus. There were significantly increases in the adrenal weight/b. w. raio of 30-day-old rats that were treated with 3%, 12%, and 48% Ji-Pei-Jing. The rats treated with 48% Ji-Pei-Jing had significantly lesser ovary weight.b.w. radio on day 30, too. The rats treated with Ji-Pei-Jing could normally ovulate at the first estrus, and no significant differences were observed during estrous *** effects of PCE on serum levels of K, P, LH in 30-day-old nds and FSH in 28-day-old ras were elevated significantly by 3 % Ji-Pei-Jing treatment. It appeare that the effects of PCE result from interaction of contained complex physiologically active substances. Steriods, especially estradio-17β,possibly Play a key role, and polpeptide hormones may also exert important effects.

关键词： Effects of Products of Chicken Embryo on Growth and Sexual Development in Rats LHRH

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