检索结果-南通市图书馆

Expression,Characterization and Immunogenicity of a Major Outer Membrane Protein from Vibrio alginolyticus

Acta Biochimica et Biophysica Sinica 2007年第3期39卷 194-200页

作者： Ronghua QIAN~(1,2) Wuying CHU~3 Zhijuan MAO~(1,4) Chongwen ZHANG~(1,2) Yongwei WEI~2 Lian YU~2* 1 Department of Biomedical Engineering,zhejiang university,hangzhou 310027,china 2 institute of preventive veterinary medicine,zhejiang university,hangzhou 310029,china 3 institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,china 4 Ningbo Oceanography and Fishery institute,Ningbo 315012,china Department of Biomedical Engineering Zhejiang UniversityHangzhou 310027 China institute of preventive veterinary medicine Zhejiang UniversityHangzhou 310029 China institute of Subtropical Agriculture Chinese Academy of SciencesChangsha 410125 China Ningbo Oceanography and Fishery instituteNingbo 315012 China

Vibrio alginolyticus is one of the Vibrio pathogens common to humans and marine *** infection and induction of the host immune response,outer membrane proteins of bacteria play animportant *** this study,an outer membrane protein gene(ompW)was cloned from *** andexpressed in Escherichia *** 645 bp open reading frame(ORF)encodes a protein of 214 amino acidresidues with a predicted molecular weight of 23.3 *** amino acid sequence showed a high identitywith that of Photobacterium damselae(96.2%)and Vibrio parahaemolyticus(94.4%).The alignment analy-sis indicated that OmpW was highly *** dodecyl sulfate-polyacrylamide gel electrophoresisshowed that the gene was over-expressed in *** BL21(DE3).Western blot analysis revealed that theexpressed protein had *** recombinant protein was purified by affinity chromatographyon Ni-NTA Superflow *** yellow croaker vaccinated with the purified OmpW showed significantlyincreased antibody to OmpW,which could resist the infection by ***.A specific antibody wasdetected by enzyme-linked immunosorbent *** study suggested that the conserved OmpW could bean effective vaccine candidate against infection by ***.

关键词： Vibrio alginolyticus ompW expression characterization immunogenicity vaccine candidate

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synthesis of Reassortant Infectious Bursal Disease Virus in Chickens Injected Directly with Infectious Clones from Different Virus Strains

引用

Acta Biochimica et Biophysica Sinica 2005年第3期37卷 192-198页

作者： Long LI Yao-Wei HUANG Lian-Sheng WANG Wang-Jun WAN Lian YU zhejiang Provincial Key Laboratory of preventive veterinary medicine,institute of preventive veterinary medicine.zhejiang university.hangzhou 310029,china zhejiang Provincial Key Laboratory of preventive veterinary medicine Institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China

The infectious bursal disease virus（IBDV）,a member of the Birnaviridae family,containinga bisegmented double-stranded RNA genome,encodes four structural viral proteins,VP1,VP2,VP3,andVP4,as well as a non-structural protein,VP5,In the present paper,the segment A from two IBDV strains,field isolate ZJ2000 and attenuated strain HZ2,were inserted into one NaeI site by site-directed silent mu-tagenesis and subcloned into the eukaryotic expression plasmid pCI under the control of the human cy-tomegalovirus（hCMV）immediate early enhancer and promoter to construct the recombinant plasmids pCI-AKZJ2000and pCI-AKHZ2,*** of the two recombinants was combined with another recombinant pCIplasroid containing the marked segment B of strain HZ2（pCI-mB）,and injected intramuscularly into non-immunized *** chimeric IBDV strains were recovered from the *** out of eightchickens in each of two groups showed the bursal histopathological *** reassortant virus derivedfrom pCI-AKZJ2000/pCI-mB can infect chicken embryos and shows relatively low *** havedeveloped a novel virus reverse genetic approach for the study of *** results also form the basis forinvestigating the role of VP1 in viral replication and pathogenecity.

关键词： infectious bursal disease infectious bursal disease virus reverse genetics reassortant virus animal model

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Virulence Phenotyping and Molecular Characterization of a Low-pathogenicity Isolate of Listeria monocytogenes from Cow's Milk

引用

Acta Biochimica et Biophysica Sinica 2006年第4期38卷 262-270页

作者： Ling-Li JIANG Jing-Jing XU Ning CHEN Jiang-Bing SHUAI Wei-Huan FANG* institute of preventive veterinary medicine,zhejiang Provincial Key Laboratory of preventive veterinary medicine,zhejiang university,hangzhou 310029,china institute of preventive veterinary medicine Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China

A low-pathogenicity isolate of Listeria monocytogenes from cow’s milk,as screened in mouseand chicken embryonated egg models,was examined for virulence-related phenotypic *** genes (iap,prfA,plcA,hly,mpl,actA,plcB,InlA and InlB) were compared with *** strains 10403S and EGD to elucidate the possible molecular mechanisms of low ***-though *** H4 exhibited similar patterns to strain 10403S in terms of hemolytic activity,invitro growth and invasiveness and even had higher adhesiveness,faster intracellular growth and higherphospholipase activity in vitro,it was substantially less virulent than the strain 10403S in mouse and chickenembryo models (50% lethal dose:10vs.10and 10vs.10,respectively).The genes prfA,plcA andmpl were homologous among *** strains H4,10403S and EGD (>98%).Genes iap,hly,plcB,InlA and InIB of *** 10403S had higher homology to those of strain EGD (>98%) than *** homology of the gene hly between strain 10403S and isolate H4 was 96.9% at the nucleotide level,but 98.7% at the amino acid *** actA gene of isolate H4 had deletions of 105 nucleotides correspondingto 35 amino acid deletions falling Within the proline-rich *** together,this study presents someclues as to reduced virulence to mice and chicken embryos of the isolate H4 probably as a result of deletionmutations of actA.

关键词： Listeria monocytogenes virulence factor virulence-associated gene

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Nitric Oxide Inducing Function and Intracellular Movement of Chicken Interleukin-18 in Cultured Cells

引用

Acta Biochimica et Biophysica Sinica 2005年第10期37卷 688-693页

作者： Jian XU~(1,3) Tong-Le DENG~2 Long LI~1 Zhen-Qiang YOU~1 Wang-Jun WAN~2 Lian YU~(1*) 1 zhejiang Provincial Key Laboratory of preventive veterinary medicine.institute of preventive veterinary medicine,zhejiang university,hangzhou 310029.china 2 College of Biomedical Engineering and Instrument Science,zhejiang university,hangzhou 310029,china 3 Medical College of Shihezi university,Xinjiang 832002,china zhejiang Provincial Key Laboratory of preventive veterinary medicine Institute of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China Medical College of Shihezi universityXinjiang 832002 China College of Biomedical Engineering and Instrument Science Zhejiang UniversityHangzhou 310029 China

To evaluate the characteristics of chicken interleukin-18 (ChIL-18) in different forms in vitro,the ChIL-18 full-length gene (ChIL-18-F) and the ChIL-18 presumed mature protein gene (ChIL-18-M) werecloned and inserted into the eukaryotic expression vector pCI,to construct recombinant pCI-ChIL-18-Fand *** recombinant plasmids were then transferred into chicken splenic lymphocytes(CSLs).Western blot showed that ChIL-18-F,with a molecular weight of 23.0 kDa,was produced in CSLstransfected by pCI-ChlL-18-F;ChIL-18-M,with a molecular weight of 19.5 kDa,was produced in CSLstransfected by *** nitric oxide (NO) level in the transfected CSLs and the culture mediumat different time points was further examined under confocal microscopy using *** results showed that both pCI-ChIL-18-F and pCI-ChIL-18-M groups showed significantincrease in intracellular and extracellular NO production compared with pCI transfected control *** suggest that both ChIL-18-F and ChIL-18-M could stimulate NO secretion in *** characterizethe intracellular distribution of ChIL-18,ChIL-18-F and ChIL-18-M were each fused to the enhanced greenfluorescent protein gene,and expressed in Vero *** results showed that the ChIL-18-F tended to themembranous region in Vero cells,while ChIL-18-M did *** indicates that the N-terminal 27 amino acidpeptide helped ChIL-18 target to Veto cell membranes.

关键词： chicken interleukin-18 N-terminal peptide nitric oxide splenic lymphocyte intracellular movement

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Listeria monocytogenes Mutants Carrying Newcastle Disease Virus F Gene Fused to its actA and plcB:In vitro Expression and Immunogenicity in Chickens

引用

Acta Biochimica et Biophysica Sinica 2007年第1期39卷 57-66页

作者： Lingli JIANG Chunlin KE Jingjing XU Jianshun CHEN Xueyan CHEN Ning CHEN Jiangbing SHUAI and Weihuan FANG institute of preventive veterinary medicine,and zhejiang Provincial Key Laboratory for Preventve veterinary medicine,zhejiang university,hangzhou 310029,china institute of preventive veterinary medicine and Zhejiang Provincial Key Laboratory for Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China

Recombinant Listeria monocytogenes mutants carrying Newcastle disease virus (NDV) fusionprotein gene F were constructed by homologous *** F or its truncated fragment Fa wasused as the model heterologous gene to be integrated into actA or plcB downstream of their signal *** orientation of the inserted genes was verified by polymerase chain reaction amplification of F or *** inserted F and Fa were expressed in the two recombinants Lm-AactA-F and Lm-AplcB-Fa as shown bysodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western *** recombinantsexhibited reduced virulence to embryonated eggs and mice by about 1.5-2.5 logs as compared with theparent wild strain 10403S. They were also less invasive than strain 10403S (P<0.05). Chickens receiving therecombinant strains orally or intraperitoneally were partially protected from virulent NDV challenge possiblydue to enhancement of non-specific immunity because the antibody titers against the homologous virus strainor the recombinant truncated fusion protein were marginal. Further research is needed in other animal modelsto see if the low antibody response results from insufficient expression of the heterologous genes as a resultof failure of L. monocytogenes or its recombinants to persist or replicate in chickens.

关键词： Listeria monocytogenes homologous recofflbination Newcastle disease virvs fusion protein F heterologous gene expression immunogenicity

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Interaction between a Nanovirus-like Component and the Tobacco Curly Shoot Virus/Satellite Complex

引用

Acta Biochimica et Biophysica Sinica 2005年第1期37卷 25-31页

作者： Pei-Jun WU Xue-Ping ZHOU~* institute of Biotechnology zhejiang university,hangzhou 310029,china institute of Biotechnology Zhejiang UniversityHangzhou 310029 China

The biological role of DNA1,a nanovirus-like component shown to be associated with thebegomovirus/satellite complex,has not yet been ***,we demonstrated that DNAl of Tobaccocurly shoot virus isolate Y35(TbCSV-Y35)attenuated leaf-curling symptoms induced by TbCSV-Y35 orTbCSV-Y35 plus Y35 DNAβ in the early stage of symptom development and induced leaf cluster at a laterstage of symptom development in Nicotiana benthamiana *** leaf disc assay demonstrated thatTbCSV-Y35 DNA 1 replicated *** blot analysis revealed that TbCSV-Y35 DNA 1 re-duced viral DNA *** DNA accumulation was not reduced when plants were co-inoculatedwith TbCSV-Y35 DNAβ,but the TbCSV-Y35 DNAβ level was dramatically reduced in the presence ofTbCSV-Y35 DNA *** determine whether the interaction between TbCSV/satellite complex and DNA 1 hadisolate specificity,DNAI of TbCSV isolate Y132 was cloned and *** was found to have 75%nucleotide sequence identity with TbCSV-Y35 DNA *** tests showed that TbCSV-Y 132 DNA 1 hadno effect on the symptoms induced by TbCSV-Y35 or TbCSV-Y35 and Y35 DNAβ in ***,although Y 132 DNA1 could replicate in these plants.

关键词： Tobacco curly shoot virus DNA1 DNAβ symptom replication interaction

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Identification of a bi-directional promoter from Tomato yellow leaf curl china virus

引用

Progress in Natural Science:Materials International 2006年第3期16卷 281-286页

作者： GUAN Cuiping and ZHOU Xueping (institute of Biotechnology, zhejiang university, hangzhou 310029, china) institute of Biotechnology Zhejiang University Hangzhou 310029 China

A bi-directional promoter of Tomato yellow leaf curl china virus (TYLCCNV) was obtained with the total DNA from TYLCCNV isolate Y10 infected tobacco leaves as a template. Plant expression vectors were constructed by fusing the amplified DNA fragment with the gus gene and nopaline terminator in different orientations. The vectors containing promoter fragments were transferred into leaf cells and plant stems of Nicotiana benthamiana by Agrobacterium-mediated method. Transient expression results showed that both the complementary and virion-sense promoters could drive the gus gene to express, and the GUS activity of the complementary-sense promoter was stronger than that of the virion-sense. Co-expression of the vector containing βC1 gene of TYLCCNV DNAβ with the vector containing a bi-directional promoter revealed that the βC1 protein has no impact on expression of either the virion- or the complementary-sense promoter.

关键词： Tomato yellow leaf curl china virus bi-directional promoter transient expression geminivirus.

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Recombinant Functional Human Lactoferrin Expressed in Baculovirus System

引用

Acta Biochimica et Biophysica Sinica 2006年第3期38卷 201-206页

作者： Tao LIU~(1,2) Yao-Zhou ZHANG~2* Xiang-Fu WU~31 institute of Biochemistry,College of Life Sciences,zhejiang university,hangzhou 310029,china 2 institute of Biochemistry,zhejiang Sci-Tec university,hangzhou 310018,china 3 institute of Biochemistry and Cell Biology,Chinese Academy of Sciences Shanghai 200031,china institute of Biochemistry College of Life Sciences Zhejiang UniversityHangzhou 310029 China institute of Biochemistry Zhejiang Sci-Tec UniversityHangzhou 310018 China institute of Biochemistry and Cell Biology Chinese Academy of SciencesShanghai 200031 China

Human lactoferrin (hLf) is a multifunctional iron-binding *** this study,weamplified hLfcDNA by reverse transcription-polymerase chain reaction from normal human mammary *** nucleotide sequence of the hLf was identical to the known *** constructed a recombinant virus,vBm-hLf,harboring the hLf gene and exploited the BmN cells as host to produce recombinant human lactoferrin(rhLf).It was found that a recombinant protein with a molecular mass of approximately 78 kDa was *** 13.5μg rhLf was purified from 1-2×10～5 BmN cells infected by vBm-hLf and the rhLfproved to be biologically *** method established in our study will pave the way for efficient productionof rhLf for further application of this protein in the future.

关键词： bacteriostatic activity baculovirus Bombyx mori human lactoferrin reverse transcriptionpolymerase chain reaction

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Highly Sensitive Fluorescent-labeled Probes and Glass Slide Hybridization for the Detection of Plant RNA Viruses and a Viroid

引用

Acta Biochimica et Biophysica Sinica 2007年第5期39卷 326-334页

作者： Zhiyou DU~(1,2), Bo JIN~3, Wenhong LIU~4, Liang CHEN~1, and Jishuang CHEN~(2,1*) 1 College of Life Sciences, zhejiang university, hangzhou 310029, china 2 institute of Bioengineering, zhejiang Sci-Tech university, hangzhou 310018, china 3 College of Life Sciences, zhejiang Chinese Medical university, hangzhou 310053, china 4 College of Bioengineering, zhejiang Chinese Medical university, hangzhou 310053, china College of Life Sciences Zhejiang UniversityHangzhou 310029 China institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China College of Life Sciences Zhejiang Chinese Medical UniversityHangzhou 310053 China College of Bioengineering Zhejiang Chinese Medical UniversityHangzhou 310053 China

In this study, a modified method of the conventional RNA dot-blot hybridization was established, by replacing32p labels with CY5 labels and replacing nylon membranes with positive-charged glass slides, for detecting plant RNA viruses and a viroid. The modified RNA dot-blot hybridization method was named glass slide hybridization. The optimum efficiency of RNA binding onto the surfaces of activated glass slide was achieved using aminosilane-coated glass slide as a solid matrix and 5×saline sodium citrate （SSC） as a spotting solution. Using a CY5-labeled DNA probe prepared through PCR amplification, the optimized glass slide hybridization could detect as little as 1.71 pg of tobacco mosaic virus （TMV） RNA. The sensitivity of the modified method was four times that of dot-blot hybridization on nylon membrane with a32P-labeled probe. The absence of false positive within the genus Potyvirus [potato virus A, potato virus Y （PVY） and zucchini yellow mosaic virus] showed that this method was highly specific. Furthermore, potato spindle tuber viroid （PSTVd） was also detected specifically. A test of 40 field potato samples showed that this method was equivalent to the conventional dot-blot hybridization for detecting PVY and PSTVd. To our knowledge, this is the first report of using dot-blot hybridization on glass slides with fluorescent-labeled probes for detecting plant RNA viruses and a viroid.

关键词： glass slide hybridization fluorescent labeling dot-blot hybridization virus viroid

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Isolation and Partial Characterization of a Novel Pollen-specific cDNA with Multiple Polyadenylation Sites from Wheat

引用

Acta Biochimica et Biophysica Sinica 2004年第7期36卷 467-476页

作者： Yong-Feng JIN* and Teng-Fei BIANinstitute of Biochemistry, zhejiang university, hangzhou 310029, china institute of Biochemistry Zhejiang UniversityHangzhou 310029 China

A novel pollen-specific full-length cDNA clone PSG076 was isolated using suppression subtractive hybridization and 5’/3’ RACE techniques. PSG076 was shown to exhibit multi-site polyadenylation by sequencing the 3’ ends of the cDNAs. At least six transcripts with different length were produced from the single gene based on different poly(A) tail attachment sites. However, polyadenylation consensus sequence AAUAAA was not seen at the 3’-untranslated sequence. PSG076 contained a 299 bp 5’ untranslated region and an open reading frame of 663 bp encoding a 221 amino acid peptide with pI of 4.31. A blast search revealed that this sequence did not show a significant similarity to any genes deposited in the public database. Southern blot indicated that PSG076 was a single copy gene. Northern blot and RT-PCR analysis indicated that PSG076 transcripts showed specific expression in mature pollen, and weak or undetectable signals in uninucleate microspore, immature seed, stem, young leave, root and ovary. Further analysis of the expression pattern in gametophyte showed that PSG076 transcripts were undetectable in uninucleate, binucleate microspore and pollen at early stage, and were first detectable and increased rapidly at middle and late stages of pollen development with the maximum level in mature pollen and also expressed in germinating pollen in vivo, suggesting that PSG076might play a role in pollen germination and pollen tube growthin addition to its function in maturation. The evidences gathered in this work indicated that the six different transcripts from the single gene were differentially expressed during pollen development.

关键词： wheat (Triticum aestivum L.) pollen-specific cDNA suppression subtractive hybridization multiple-site polyadenylation differential expression

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：