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Artificial Induction of Twinning by an Active Immunization of Beef Cows Against Inhibin Partially Purified from Porcine Seminal Plasma

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agricultural sciences in china 2002年第4期1卷 466-471页

作者： YANG Li-guo, ZHANG Ju-nong, WANG Jin-rong, YE Rong, SANG Run-zi, NIU Shu-li and LIU Cheng-hai( Research Laboratory of animal Reproduction , College of animal science and technology, Nanjing agricultural university,Nanjing 210095 , china College of animal science and technology, Shihezi agricultural university, Shihezi 832003 ,china Department of animal Husbandry and Veterinary Medicine , Xinjiang agricultural university,Wulumuqi 830000 , china Department of animal Husbandry and Veterinary Medicine ,Hebei agricultural university, Baoding 071001 , P.R. china) Research Laboratory of animal Reproduction College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China College of animal science and technology Shihezi Agricultural University Shihezi 832003 China Department of animal Husbandry and Veterinary Medicine Xinjiang Agricultural University Wulumuqi 830000 China Department of animal Husbandry and Veterinary Medicine Hebei Agricultural University Baoding 071001P.R. China

Two hundred and seventy multiparous Chinese Yellow cattle (beef) were selected at 1 to 3 months postpartum and divided into three groups (90 cows for each). animals were given both a primary and booster immunizations with a total dose of 3 mg (Group Th) or 1.5 mg (Group Tl) of seminal preparation containing inhibin activity, emulsified with Freund's complete adjuvant and incomplete adjuvant (for booster) , at 3 or 4-week intervals. Other cows were treated with the same volume of seminal preparation without inhibin activity as procedures mentioned above to serve as a control (Group C). Artificial inseminations were given twice at 8 - 12 h intervals when the cow was in heat. Jugular venous blood samples were collected from each cow and used to assay the presence of antibody against seminal preparation by double-diffusion in agar precipitation test and to detect the titer of inhibin antibody by an ELISA method. Data from 247 cows showed that 83.9% (73/87) of cows were in estrus and ovulated 89 ova altogether, of which 19 cows ovulated twin ova and 15 cows produced twins in Group Th (n = 87). However, only 61.1% (44/72) of cows in Group TI (n = 72) and 62.5% (55/88) of cows in Group C were in estrus and ovulated 46 and 52 ova altogether respectively. The ovulation rate (1.27 ± 0.03), calving rate (126.3%) and twinning rate (26.3%) in Group Th were greater than those in Groups Tl or C (Panimals (r = 0.7507, P<0.01). These results indicate that active immunization of postpartum cows against inhibin purified from porcine seminal plasma may increase the ovulation rate and induce twinning, suggesting the potential to develop a method to improve fertility in cows.

关键词： Beef cows Seminal inhibin Active immunization Twinning Ovulation rate

Anti-fatigue Function of Schizophyllum commune in Mice

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食用菌学报 2006年第3期 45-47页

作者： HAO Rui-fang, LI Rong-chun Institute of Edible Fungi, yunnan agricultural university, kunming, yunnan 650201, china

Levels of liver and muscle glycogen, blood urea nitrogen (BUN), blood lactic acid (LD), succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) were determined in mice that were fed diets containing either Schizophyllum commune fruit body powder or submerged cultures of the fungus. Liver glycogen levels, and SDH and LDH levels in the serum of mice fed diets containing mushroom fruit bodies or submerged culture material were significantly higher (P<0.01) compared with control mice fed basal feed only. Muscle glycogen levels were also significantly higher (P<0.01) in mice fed fruit body-containing diets than in control mice. Compared with controls, LD elimination rates were higher, and increases in BUN were significantly lower (P<0.01), in mice fed diets containing either mushroom fruit bodies or submerged culture material. These data indicate a clear anti-fatigue function for S. commune.

关键词： Schizophyllum commune Anti-fatigue Glycogen Succinate dehydrogenase Lactic dehydrogenase Urea nitrogen Lactic acid

Molecular characterization and expression analysis of caveolin-1 in pig tissues

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science china(Life sciences) 2008年第7期51卷 655-661页

作者： WANG Chong1,MEI YingJie 1,LI Li 1,MO DeLin 2,LI JiaQi 1,ZHANG Hao 1,TIAN XingGuo 1 &CHEN YaoSheng2 1College of animal science,South china agricultural university,Guangzhou 510642,china 2School of Life science,Sun Yat-Sen university,Guangzhou 510006,china 1. College of animal science South China Agricultural University Guangzhou 510642 China 2. School of Life science Sun Yat-Sen University Guangzhou 510006 China

Members of the caveolin family played important roles during fundamental cellular processes,such as regulation of cell morphology,migration,and gene expression in muscle *** this study,caveolin-1 (Cav-1),one of the caveolins,was identified from longissimus dorsi muscle of Large Yorkshire pig and Chinese indigenous Lantang pig based on the results of mRNA differential display *** deduced amino acids sequence of the porcine Cav-1 contained a caveolin domain,and was very conservative among different *** Cav-1 mRNA was widely expressed in the eight tissues in this study,including heart,liver,kidney,encephalon,spleen,lung,longissimus dorsi muscle,and back fat, and the highest expression quantity was found in back fat of the two pig *** expression quantity of porcine Cav-1 in back fat and longissimus dorsi muscle of Lantang pig was significantly higher than that of Large Yorkshire(Panimal model of pig.

关键词： caveolin family clone expression analysis real-time quantify PCR

Layer-by-layer assembly carbon nanotubes thin film based gas sensors for ammonia detection

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science china(Information sciences) 2011年第12期54卷 2700-2706页

作者： SUN Ping 1,2 , JIANG YaDong 1* , XIE GuangZhong 1 , DU XiaoSong 1 , LI Xian 1 & HU Jia 1 1 School of Optoelectronic Information, State Key Laboratory of Electronic Thin Films and Integrated Devices, university of Electronic science and technology of china (UESTC), Chengdu, 610054, china 2 Life and Physical science College, Sichuan agricultural university, Ya’an 625014, china 1. School of Optoelectronic Information State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China (UESTC) Chengdu 610054 China2. Life and Physical Science College Sichuan Agricultural University Ya’an 625014 China

Single walled carbon nanotubes (SWNTs) were deposited on interdigital electrode (IDE) devices and quartz crystal microbalance (QCM) devices through layer-by-layer self-assembly method to fabricate resistance sensitive and mass sensitive gas sensors, respectively. Their ammonia gas-sensitive characteristics were detected at different temperature. It is revealed that the SWNTs based mass sensitive gas sensor has good sensing properties at room temperature. However, the SWNTs based resistance sensitive gas sensor has irreversible response for ammonia gas. When the temperature increases, the noise of SWNTs based mass sensitive gas sensor increases and the gas sensitive characteristic decreases. Meanwhile, the humidity effect is also discussed. It is important to optimize gas sensor by making it work at an appropriate external environment.

关键词： layer-by-layer self-assembly carbon nanotube gas sensor

Nonsingularity in second-order cone programming via the smoothing metric projector

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science china Mathematics 2010年第4期53卷 1025-1038页

作者： WANG Yun 1,& ZHANG LiWei 2 1 College of Information sciences and Engineering,Shandong agricultural university,Tai’an 271018,china 2 Department of Applied Mathematics,Dalian university of technology,Dalian 116024,china 1. College of Information sciences and Engineering Shandong Agricultural University Tai’an 271018 China2. Department of Applied Mathematics Dalian University of Technology Dalian 116024 China

Based on the differential properties of the smoothing metric projector onto the second-order cone,we prove that,for a locally optimal solution to a nonlinear second-order cone programming problem,the nonsingularity of the Clarke's generalized Jacobian of the smoothing Karush-Kuhn-Tucker system,constructed by the smoothing metric projector,is equivalent to the strong second-order sufficient condition and constraint nondegeneracy,which is in turn equivalent to the strong regularity of the Karush-Kuhn-Tucker ***,this nonsingularity property guarantees the quadratic convergence of the corresponding smoothing Newton method for solving a Karush-Kuhn-Tucker ***,the analysis does not need the strict complementarity condition.

关键词： second-order cone programming problem smoothing metric projector B-subdifferential Clarke’s generalized Jacobian smoothing Newton method

Identification of yak lactate dehydrogenase B gene variants by gene cloning

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science china(Life sciences) 2008年第5期51卷 430-434页

作者： ZHENG YuCai1, ZHAO XingBo2, ZHOU Jing1, PIAO Ying1, JIN SuYu1, HE QingHua1, HONG Jian1, LI Ning2 & WU ChangXin2 1 College of Life science and technology, Southwest university for Nationalities, Chengdu 610041, china 2 State Key Laboratory for Agrobiotechnology and Ministry of Agriculture Key Laboratory of animal Genetics and Breeding, College of animal science and technology, china agricultural university, Beijing 100094, china College of Life science technology Southwest University for Nationalities Chengdu 610041 China State Key Laboratory for Agrobiotechnology Ministry of Agriculture Key Laboratory of animal Genetics Breeding College of Animal Science technology China Agricultural University Beijing 100094 China

Native polyacrylamide gel electrophoresis showed that two types of lactate dehydrogenase (LDH) existed in yaks. Based on the electrophoresis characteristics of LDH isoenzymes, yak LDH variants were speculated to be the gene mutation on H subunit encoded by B gene. According to the mobility in electrophoresis, the fast-band LDH type was named LDH-Hf and the slow-band LDH type LDH-Hs. In order to reveal the gene alteration in yak LDH variants, total RNA was extracted from heart tissues of yaks with different LDH variants, and cDNAs of the two variants were reverse transcripted. Two variants of B genes were cloned by RT-PCR. Sequence analysis revealed that four nucleotides differed between LDH-Bf and LDH-Bs, which resulted in two amino acids alteration. By Deepview software analysis of the conformation of yak LDH1 variants and H subunit, these four nucleotides altered two amino acids that generated new hydrogen bonds to change the hydrogen bonds network, and further caused subtle conformational changes between the two LDH variants.

关键词： yak lactate dehydrogenase genetic variants gene cloning molecular modeling

Effects　of　alkaline　ameliorator　additions　on　the　acidic　buffering　abilities　of　aluminum－rich　alfisols

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Journal of Environmental sciences 1996年第2期8卷 235-241页

作者： Wu Jiemin Shao Feng(Department of Environmental science, Zhejiang agricultural university, Hangzhou 310029 , china) Department of Environmental science Zhejiang Agricultural University Hangzhou 310029 China

Lime and coal flying ash applications could mitigate pollution from acid deposition and improve the buffering ability of two acidic soils sampled from Zhejiang Province. The results showed that soil alkalinity and acidified buffering capacities (QpHs ) were significantly increased, especially for liming treatment; whereas, the amounts of SO24- adsorbed by treated soils were decreased to some degrees because net negative charges of soil oxide colloidal surfaces were increased with the decrease in soil acidity, although the soil fixation abilities for adsorbed SO24- were not reduced. These facts indicated that after the alkaline ameliorators were applied, the positive and adverse effects resulted from sulfate-type acid deposition were simultaneously existed in these soil treatments.

关键词： acidic alfisol alkaline ameliorator acidified buffering capacity.

Polymorphisms of three gene-derived STS on pig chromosome 13q41 are associated with susceptibility to enterotoxigenic Escherichia coli F4ab/ac in pigs

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science china(Life sciences) 2008年第7期51卷 614-619页

作者： HUANG Xiang 1 ,REN Jun 1 ,YAN XueMing 1,2 ,PENG QiuLing 1 ,TANG Huan 1 ,ZHANG Bo 1 ,JI HuaYuan 1 , YANG ShuJin1&HUANG LuSheng 1 1Key Laboratory for animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of china,Jiangxi agricultural university, Nanchang 330045,china 2Department of Biology,Nanchang university of science and technology,Nanchang 330038,china 1. Key Laboratory for animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of china Jiangxi Agricultural University Nanchang 330045 China 2. Department of Biology Nanchang University of Science and Technology Nanchang 330038 China

Neonatal diarrhea caused by enterotoxigenic Escherichia coli(ETEC)F4 is a common and serious disease,resulting in significant economical loss in the pig *** locus encoding ETEC F4 receptor has been mapped to pig chromosome(SSC)13q41,and one of the most significantly linked markers is *** this study,we selected three genes including SLC12A8,MYLK and KPNA1 from a chromosomal region flanking S0075 on SSC13 to develop pig specific sequence tagged sites(STS). Seven single nucleotide polymorphisms were identified in the three pig STS using DNA of four full-sib susceptible and resistant animals in a White Duroc×Erhualian *** grandparents,parents and 755 offspring in the intercross were genotyped for three polymorphisms,including SLC12A8 g.159A>G,MYLK g.1673A>G and KPNA1 g.306A>***-based transmission disequilibrium test(TDT) revealed that all polymorphisms and the corresponding haplotypes are significantly associated with ETEC F4ab/ac(especially F4ac)brush border adhesion phenotypes,indicating that these polymor- phism are in linkage disequlibrium with causal mutation(s)of the gene encoding ETEC F4ab/ac receptor. Our results strengthen the evidence for the involvement of SSC13q41 in high acquiring risk of ETEC F4ab/ac infection,and provide novel polymorphic markers for fine mapping of the ETEC F4ab/ac receptor locus.

关键词： pig SSC13q41 sequence tagged sites(STS) Escherichia coli(ETEC)F4ab/ac susceptibility

Key Parameters and Conceptual Configuration of Unmanned Combat Aerial Vehicle Concept

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Chinese Journal of Aeronautics 2009年第4期22卷 393-400页

作者： Wang Ganglin faculty 513,School of Aeronautic science and Engineering,Beijing university of Aeronautics and Astronautics,Beijing 100191,china [a]faculty 513 School of Aeronautic Science and Engineering Beijing University of Aeronautics and Astronautics Beijing 100191 China

The nature and characteristics of attack unmanned combat aerial vehicle （UCAV） are analyzed. The principles of selecting takeoff thrust-weight ratio and takeoff weight of attack UCAV are presented by analyzing the statistical data of weights for various main combat aircraft. The UCAV airborne weapons are analyzed, followed by the preliminary estimation of the payload weight. Various typical engines are analyzed and one of them is selected. Then the takeoff weight of the UCAV is determined. Based on some basic parameters and assumptions, the qualitative decomposition calculation for takeoff weight is completed. The key factors for obtaining longer endurance of aircraft with small aspect ratio configuration are found to be high lift-drag ratio and internal space. On the basis of the conclusions mentioned above, a highly blended flying-wing plus lifting body concept is proposed. According to this concept, the UCAV configuration is designed and optimized. Finally, the UCAV configuration with small aspect ratio, high lift-drag ratio, and high stealth characteristic is obtained.

关键词： aircraft design unmanned aerial vehicles unmanned combat aerial vehicles conceptual design aerodynamicsstealth