Polymorphisms of three gene-derived STS on pig chromosome 13q41 are associated with susceptibility to enterotoxigenic Escherichia coli F4ab/ac in pigs

作     者：HUANG Xiang 1 ,REN Jun 1 ,YAN XueMing 1,2 ,PENG QiuLing 1 ,TANG Huan 1 ,ZHANG Bo 1 ,JI HuaYuan 1 , YANG ShuJin1&HUANG LuSheng 1 1Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China,Jiangxi Agricultural University, Nanchang 330045,China 2Department of Biology,Nanchang University of Science and Technology,Nanchang 330038,China 

作者机构：Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China Jiangxi Agricultural University Nanchang China Department of Biology Nanchang University of Science and Technology Nanchang China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2008年第51卷第7期

页      面：614-619页

核心收录：

学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0906[农学-兽医学] 

基　　金：National High Technology Research and Development Program of China("863"Program)(Grant No.2006AA10Z194) Jiangxi Provincial KeyResearch Project 

主　　题：pig SSC13q41 sequence tagged sites(STS) Escherichia coli(ETEC)F4ab/ac susceptibility 

摘      要：Neonatal diarrhea caused by enterotoxigenic Escherichia coli(ETEC)F4 is a common and serious disease,resulting in significant economical loss in the pig *** locus encoding ETEC F4 receptor has been mapped to pig chromosome(SSC)13q41,and one of the most significantly linked markers is *** this study,we selected three genes including SLC12A8,MYLK and KPNA1 from a chromosomal region flanking S0075 on SSC13 to develop pig specific sequence tagged sites(STS). Seven single nucleotide polymorphisms were identified in the three pig STS using DNA of four full-sib susceptible and resistant animals in a White Duroc×Erhualian *** grandparents,parents and 755 offspring in the intercross were genotyped for three polymorphisms,including SLC12A8 g.159AG,MYLK g.1673AG and KPNA1 g.306A***-based transmission disequilibrium test(TDT) revealed that all polymorphisms and the corresponding haplotypes are significantly associated with ETEC F4ab/ac(especially F4ac)brush border adhesion phenotypes,indicating that these polymor- phism are in linkage disequlibrium with causal mutation(s)of the gene encoding ETEC F4ab/ac receptor. Our results strengthen the evidence for the involvement of SSC13q41 in high acquiring risk of ETEC F4ab/ac infection,and provide novel polymorphic markers for fine mapping of the ETEC F4ab/ac receptor locus.