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World Journal of Gastroenterology 2003年第8期9卷 1739-1742页

作者： Wan-Fu Zhu Li-Min Yin Peng Li Jian Huang Hui Zhuang, department of Microbiology, school of basic medical sciences, Peking university, Beijing 100083, china Wan-Fu Zhu Li-Min Yin Peng Li Jian Huang Hui Zhuang Department of Microbiology School of Basic Medical Sciences Peking University Beijing 100083 China

AIM: To determine the pathogenicity of GB virus C (GBV-C)on liver and the effects of its co-infection on the clinical features and prognosis of patients with hepatitis B and ***: Cross-sectional study was carried out in 413patients with acute, chronic hepatitis B or liver cirrhosis,and in 67 hemodialysis patients. A 20-month prospective cohort study was carried out in 95 hepatitis B and 80 hepatitis C patients. A reverse transcriptase nested polymerase chain reaction (RT-nPCR) of the 5′-noncoding region was used to detect circulating GBV-C RNA. Liver function was determined by an automated analyzer for all ***: The prevalence of GBV-C in the high-risk populations with the virus transmitted via blood was high,ranging from 16.2 to 28.8%. Co-infection with GBV-C in hepatitis B patients did not affect the clinical features of the disease or liver function. The dialysis patients infected with GBV-C alone did not develop functional changes to the *** cohort study showed that GBV-C co-infection did not affect the clinical features, prognosis or negative serum conversion rate of chronic hepatitis B and ***: The results suggest that GBV-C has no marked pathogenicity on liver, so it may not be a hepatitis virus.

关键词：病毒性肝炎血液透析 GB病毒病原学

Advances in mammalian spermatogonial stem cell transplantation

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Progress in Natural Science:Materials International 2004年第6期 5-9页

作者： ZHANG Xueming 1 *, LI Dexue 2, YUE Zhanpeng 1, LI Lingling 3 and YU Jiaao 4(1. Faculty of Military Veterinary,Quarter-Master university of PLA,changchun 130062,china 2. Academy of Military medical sciences,Beijing 100085,china 3. Qian Wei Hospital of jilin Province,changchun 130021,china 4. The First Hospital of jilin university,changchun 130021,china) Faculty of Military Veterinary Quarter-Master University of PLA Changchun 130062 China Academy of Military medical sciences Beijing 100085 China Qian Wei Hospital of jilin Province Changchun 130021 China The First Hospital of jilin university Changchun 130021 China

Spermatogonial stem cell (SSC) transplantation is a novel technique by which testicular cells from normal,transgenic or mutant donor are introduced into the seminiferous tubules of recipient testes through microinjection. Subsequently,donor SSCs survive,migrate,anchor and proliferate in the recipient testis,furthermore,initiate spermatogenesis and even produce sperms capable of fertilization. This technique provides a new approach for the researches of spermatogenesis mechanism,regeneration of spermatogenesis in sterile individuals and reproduction of transgenic animals. This review focuses on the methodological breakthroughs and highlights the recent findings that have substantially increased understanding of SSC biology. The article provides a comprehensive overview of this technique and its multiple applications in basic science and medicine. And the perspective direction of this field in the near future is proposed.

关键词： spermatogonial stem cell transplantation application.

低剂量辐射激活小鼠脾细胞CREB及NF-κB

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生物化学与生物物理进展 1998年第2期25卷 147-150页

作者：陈沙力刘树铮白求恩医科大学放射生物教研室

采用凝胶电泳迁移率变化分析和寡核苷酸竞争抑制方法检测低剂量Ｘ射线整体照射对小鼠脾细胞基因转录调控的影响．７５ｍＧｙＸ射线全身照射小鼠后４ｈ，脾细胞核蛋白提取物的ＣＲＥＢ及ＮＦκＢ与其基因启动部位增强子控制序列的结合... 详细信息

采用凝胶电泳迁移率变化分析和寡核苷酸竞争抑制方法检测低剂量Ｘ射线整体照射对小鼠脾细胞基因转录调控的影响．７５ｍＧｙＸ射线全身照射小鼠后４ｈ，脾细胞核蛋白提取物的ＣＲＥＢ及ＮＦκＢ与其基因启动部位增强子控制序列的结合活性较相同浓度的对照核蛋白提取物分别增强７倍及５倍．竞争抑制试验证实ＣＲＥＢ及ＮＦκＢ与其控制序列特异地结合．提示低剂量Ｘ射线全身照射选择性地激活脾细胞ＣＲＥＢ及ＮＦκＢ，通过与增强子控制序列位点的结合，特异地诱导基因转录，从而引起功能激活．

关键词：低剂量辐射脾细胞 CREB NF-kB

Changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain

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Changes of GABA-activated currents in isolated dorsal root g...

Effects of DDPH on HECCM-induced Proliferation and Immunophenotypes of the Pulmonary Vascular Pericytes

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： He ZHU,Jun-Qiang SI,Ke-Tao MA,Zhong-Shuang ZHANG,Jing LI,Li LI ~* 1 department of physiology,Shihezi university medical College,Shihezi,832002,china 2 department of physiology,Wuhan university school of basic medical sciences,Wuhan 430070,china

Objective:To investigate the changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic ***:The neuropathic pain model was established by chronic constriction injury (CCI) 7 days before electrophysiological *** rat DRG neurons were enzymatically ***-cell patch clamp technique was used to record GABA-activated *** changes of currents of injured side and opposite side were expected to compare with control ***:(1) The currents of injured side of CCI group were significantly decreased compared with control group(GABA concentration,0.1-1000μmol/L),in injured side of CCI group,the amplitude of 100μmol/ L GABA-induced currents is 0.97 nA(n= 8);in control group,the amplitude of 100μmol/L GABA-induced currents is 1.99 nA(n= 13).(2) By the contrast,opposite side currents of CCI group increased significantly compared with injured side and control group(GABA concentration,0.01-1000μmol/L).In opposite side of CCI group,the amplitude of 100μmol/ L GABA-induced currents is 4.25 nA(n= 7).Conclusion: The data indicate that the chronic constriction injury changes the function of GABA receptors of both injury side and opposite *** decrease of pre-synaptic inhibition of GABA may be a possible reason of neuropathic pain.

关键词： neuropathic pain dorsal root ganglion GABA whole-cell patch clamp

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Journal of Huazhong university of Science and Technology(medical sciences) 2001年第3期21卷 184-187页

作者：袁永辉车东媛熊密 department of Pathology School of Basic Medical Sciences Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

In order to study the effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethylamino) propane hydrochloride (DDPH) on proliferation and immunophenotypes of newborn rat pulmonary vascular pericytes induced by hypoxic endothelial cell conditioned medium (HECCM) from porcine pulmonary arteries, the cultured pericytes were divided into 4 groups according to the endothelial cell conditioned medium (ECCM) used: normoxic ECCM (NECCM) group, NECCM+DDPH group, HECCM group and HECCM+DDPH group. Cell culture, immunocytochemical staining, image analysis and flow cytometric method were used to investigate the effects of HECCM and DDPH on the expression of α-smooth muscle actin (α-SM-Actin) antigen, CD34 antigen, S-100 antigen and proliferating cell nuclear antigen (PCNA) and cell cycle in pericytes. The results showed that the α-SM-Actin antigen in the pericytes in HECCM group was stronger positively expressed than in the other three groups, but CD34 antigen and S-100 antigen were negatively expressed. The expression of α-SM-Actin antigen, CD34 antigen and S-100 antigen was positive in the groups of NECCM, NECCM+DDPH and HECCM+DDPH; The expression of α-SM-Actin and PCNA in HECCM group was 1.32 times (P24 times (P21 times (P2 %, in G2-M phase higher by 6.1 % and 4.9 % than in the groups of NECCM,HECCM+DDPH, respectively. The inhibitory rate of DDPH on the increased α-SM-Actin and PCNA syntheses in pericytes induced by HECCM were 23.4 % and 17.1 % respectively. The inhibitory rate on the increased pericytes from GO-G1 phase to S phase was 8.3 %. These results suggest that DDPH can directly inhibit pericytes from proliferation and immunophenotypical transformation of smooth muscle-like cells induced by HECCM.

关键词： DDPH pericyte immunophenotypes HECCM hypertension, pulmonary

Regulation Effect of Vascular Endothelial Growth Factor on Human Fetal Choroid Vascularization

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眼科学报 2000年第1期16卷 11-14页

作者： Jinsong Zhao, Yue Song, Yi Wang, Xiaoguang Zhangdepartment of Ophthalmology, The 2nd Clinical CollegeNorman Bethune university of medical sciences, changchun 130041, chinajilin Dispensary of Ophthalmopathy, changchun 130041, china department of Ophthalmology 2nd Clinical College Norman Bethune University of Medical Sciences Changchun 130041 China

Purpose:To investigate the spatial and temporal regulation effect of vascular endothelial growth factor(VEGF)on human fetal choroid ***:The eyeballs of 54human fetuses from the 9th week to the40th week due to accidental abortion were studied by immunohistochemically staining for the expression of VEGF and proliferation cell nuclear antigen(PCNA).Results:(1)The distribution of VEGF expression in the retinal pigment epithelium(RPE)decreased with the increase of age,the peak of which was between the 9th and 14th week,(2)PCNAimmunoreactivity was localized within choriocapillaris endothe-lium,THe expression level decreased alone with fetus age,In this period the chori-ocapillaris endothelium kept proliferation,*** and remodelled to form the choroid vessels.(3)Statistically significant correlations were shown between the expression of VEGF in the PRE and that of PCNAin choriocapillaris endothelium(r=0.933,P2000;16:11-14.

关键词：婴幼儿脉络膜血管病变血管内皮细胞生长因子临床意义

Determination of Impurity Silicon in Anticancer Drug (Ge-132) by Electrothermally Atomised Atomic Absorption Spectrometry with Optical Temperature Control Accessory

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Chemical Research in Chinese Universities 1991年第4期7卷 205-211页

作者： Xie Shujuan, Wang Daning and Zou Mingqiang (jilin I/E Commodity Inspection Bureau, changchun)Cui Xiaoyuan,Ren Yulin and Zheng Yansheng (department of Chemistry, jilin university, changchun)Wang Jincheng (Dalian Military medical school, Dalian)

The present paper describes the ashing and atomization processes in silicon analysis by electrothermally atomised atomic absorption spectrometry(EAAAS) with an uncoat-ed graphite tube, a pyrolytically coated graphite tube and a tungsten-coated graphitetube. The sensitivity and linear range of three graphite tubes were compared. By using optical temperature control accessory, the signals are enhanced by a factor of 2 and the germanium interferences in the determination of silicon are eliminated. The effects of time constant and carrier gas flow-rate on the determination of silicon were also tested. The sample can be directly analyzed in its aqueous solution without any pretreatment. The measurements of samples containing 0. 2 μg/mL and 0. 4 μg/mL silicon were run ten times and the variation coefficient is 4. 9% and 2.6%, respectively. The recovery tests for carboxyethyl germanium sesquioxide(Ge-132) synthesized and imported were performed, and the recoveries are 97. 0% and 110%, respectively. Keywords Carboxyethyl germanium sesquioxide, Electrothermally atomised atomic absorption spectrometry, Silicon

关键词： Determination of Impurity Silicon in Anticancer Drug by Electrothermally Atomised Atomic Absorption Spectrometry with Optical Temperature Control Accessory Ge-132

IL-4在诱导CD4,45RA~+和CD4,45RO~+T细胞向IL-4产生细胞分化过程中的效应

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中国免疫学杂志 1997年第1期 12-16页

作者： Wang Jiangbin,Wang Yushan,Yang Guizhen.department of Immunology,Bethune university of medical sciences,changchun 130021

应用ＥＬＩＳＰＯＴ（Ｅｎｚｙｍｅ－ｌｉｎｋｅｄｉｍｍｕｎｏｓｐｏｔａｓｓａｙ）单细胞检测技术，研究了淋巴因子在诱导ＣＤ４，４５ＲＯ＋和ＣＤ４，４５ＲＡ＋Ｔ细胞向ＩＬ－４产生细胞分化过程中的影响。在存在ＰＭＡ和ｉｏｎｏ... 详细信息

应用ＥＬＩＳＰＯＴ（Ｅｎｚｙｍｅ－ｌｉｎｋｅｄｉｍｍｕｎｏｓｐｏｔａｓｓａｙ）单细胞检测技术，研究了淋巴因子在诱导ＣＤ４，４５ＲＯ＋和ＣＤ４，４５ＲＡ＋Ｔ细胞向ＩＬ－４产生细胞分化过程中的影响。在存在ＰＭＡ和ｉｏｎｏｍｙｃｉｎ的情况下，ＩＬ－２和ＩＬ－４均可以诱导ＣＤ４，４５ＲＯ＋Ｔ细胞分化为ＩＬ－４产生细胞，但是只有ＩＬ－４可以诱导ＣＤ４，４５ＲＡ＋Ｔ细胞向ＩＬ－４产生细胞分化，ＩＦＮγ既不能诱导ＣＤ４，４５ＲＡ＋，亦不能对ＣＤ４，４５ＲＯ＋Ｔ细胞产生诱导作用。当内源性ＩＬ－４被中和后，ＩＬ－２对ＣＤ４，４５ＲＯ＋Ｔ细胞的诱导作用消失。本文结果初步表明ＩＬ－４在决定Ｔｈ前体细胞是向Ｔｈ１还是向Ｔｈ２方向发展起着关键的作用。

关键词：淋巴因子 IL-4 CD45 ELISPOT

Resveratrol attenuates brain damage in a rat model of focal cerebral ischemia via up-regulation of hippocampal Bcl-2

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Resveratrol attenuates brain damage in a rat model of focal ...