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Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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Science china(Life Sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry Science,Beijing 100097,china 2 department of chemistry and chemical engineering,hunan institute of engineering,xiangtan 411104,china 3 College of Life Science,Capital Normal University,Beijing 100037,china 4 College of Life Science,Zhongkai University of Agriculture and Technology,Guangzhou 510225,china 5 College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,china 6 department of Biology,Henan institute of Science and Technology,Xinxiang 453003,china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. department of chemistry and chemical engineering Hunan Institute of Engineering Xiangtan 411104 China 3. College of Life Science Capital Normal University Beijing 100037 China 4. College of Life Science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. College of Grassland Science Gansu Agricultural University Lanzhou 730070 China 6. department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

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Effect of static magnetic field on activity of immobilized α-amylase

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Chinese Science Bulletin 1997年第2期42卷 127-130页

作者： YAN Liushui, ZHU Yuanbao, HE-Shuang’e and CAO Zhixiang1 department of chemical engineering, Nanchang institute of Aeronautical Technology, Nanchang 330034, china 2. department of chemistry and chemical engineering, hunan University, Changsha 410082, china department of chemical engineering Nanchang Institute of Aenwututical Techndogy Nanchang China department of chemistry and chemical engineering Hunan University Changsha China

BIOLOGICAL effect of magnetic field is one of the three major subjects of contemporary biomagnetism, and is also the basis of applied biomagnetism. In order to explore magnetic biological effects, the influence of various sorts of magnetic field on many living systems as well as their different constructural levels has been extensively studied. However, only a few studies with in vitro enzyme have been reported, and few researches concerning the influence of magnetic field on immobilized enzyme reactions have been published. This note describes

关键词： static magnetic field immobilized α-amylase enzyme activity oscillatory behaviour.

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Novel nanoporous binary Ag-Ni electrocatalysts for hydrazine oxidation

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Rare Metals 2010年第1期29卷 26-31页

作者： YI Qingfeng, LI Lei, YU Wenqiang, LIU Xiaoping, ZHOU Zhihua, and NIE Huidong School of chemistry and chemical engineering, hunan University of Science and Technology, xiangtan 411201, china School of chemistry and chemical engineering Hunan University of Science and Technology

From an aqueous mixture of Ag（I）-EDTA complex and Ni（II） nitrate, silver and nickel particles were co-deposited on the surface of titanium substrates by the hydrothermal method using hydrazine hydrate as a reduction agent. The prepared titanium-supported nano-scale Ag and Ag-Ni particles （nano Ag/Ti, nano Ag86Ni14/Ti, nano Ag77Ni23/Ti, and nano Ag74Ni26/Ti） exhibit nanoporous 3D network textures. Their electrocatalytic activity towards hydrazine oxidation in alkaline solutions was evaluated by cyclic voltammetry and chronoamperometry. The results show that the four samples present a low onset potential of ca. -0.60 V vs. SCE and considerably high and stable anodic current densities for hydrazine oxidation. Among them, the nano Ag86Ni14/Ti electrode exhibits the highest anodic current density towards hydrazine oxidation, showing an increment of electro-active sites on the nano Ag86Ni14/Ti due to the addition of Ni to Ag particles.

关键词： electrocatalyst silver hydrazine oxidation fuel cell

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Resolution of α-cyclohexyl-mandelic acid enantiomers by two-phase (O/W) recognition chiral extraction

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Science china chemistry 2007年第6期50卷 764-769页

作者： TANG KeWen1,2, ZHANG GuoLi2, HUANG KeLong2, LI Yuanjian3 & YI JianMin1,2 1 department of chemistry and chemical engineering, hunan institute of Science and Technology, Yueyang 414000, china 2 College of chemistry and chemical engineering, Central South University, Changsha 410083, china 3 College of Pharmaceutical Science, Central South University, Changsha 410078, china department of chemistry and chemical engineering Hunan Institute of Science and TechnologyYueyang 414000China College of chemistry and chemical engineering Central South UniversityChangsha 410083China

This paper presents a new chiral separation technology:two-phase(O/W)recognition chiral *** behavior of alpha-cyclohexyl-mandelic acid enantiomers was studied in the extraction system with D(L)-isobutyl tartrate in 1,2-dichloroethane organic phase and beta-CD derivatives in aqueous phase,and the influence of the kind and concentration of extractant and pH on extraction performance was *** experimental results indicate that two-phase(O/W)recognition chiral extraction is of strong chiral separation ***-^-CD,HE-beta-CD and Me-beta-CD have higher recognition ability for S-CHMA than that for R-CHMA,among which HP-beta-CD has the strongest ability;whereas,D-isobutyl tartrate has reversed recognition ability for *** the extraction system containing HP-beta-CD and D-isobutyl tartrate,e.e.% of S-CHMA in aqueous phase reached 27.6% by one stage extraction,and the distribution ratio for R-CHMA(k_R)and for S-CHMA(k_S)and separation factor(alpha)are 2.44,0.89 and 2.49,***,pH and concentration of extractant have great effects on chiral separation ***-phase(O/W)recognition chiral extraction has great significance for preparative separation of racemic compounds.

关键词： two-phase(W/0)recognition chiral extraction alpha-cyclohexyl-mandelic acid enantiomers

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Empirical Criteria of Superconductivity for Some Oxides

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Journal of Materials Science & Technology 2000年第3期16卷 327-331页

作者： Xueye WANG and Huang SONG (department of chemistry, xiangtan University, xiangtan 411105, china) Guanzhou QIU and Dianzuo WANG (department of Mineral engineering, Central South University of Technology, Changsha 410083, china) department of chemistry Xiangtan University Xiangtan 411105 China department of Mineral engineering Central South University of Technology Changsha 410083 China

The properties of superconductivity of some oxides were investigated by structural parametric diagrams or pattern recognition with structural chemical parameters. The essential criteria of superconductivity for some oxides have been obtained by using 109 oxides as the training set and seven parameters as features; the results illustrated that the electronegativity difference is the most important factor among seven parameters. Moreover, the regularity of superconductive transition temperature Tc for complex oxides is discussed by partial least squares (PLS) method.

关键词： Empirical Criteria of Superconductivity for Some Oxides

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Prediction of Superconductivity for Oxides Based on Structural Parameters and Artificial Neural Network Method

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Journal of Materials Science & Technology 2000年第4期16卷 435-438页

Superconductive properties for oxides were predicted by artificial neural network (ANN) method with structural and chemical parameters as inputs. The predicted properties include superconductivity for oxides, distributed ranges of the superconductive transition temperature (Tc) for complex oxides, and Tc values for cuprate superconductors. The calculated results indicated that the adjusted ANN can be used to predict superconductive properties for unknown oxides.

关键词： Prediction of Superconductivity for Oxides Based on Structural Parameters and Artificial Neural Network Method

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An efficient chiral synthesis of (R)-N-[3-acetyl-4-(2-hydroxy-3-isopropylamino-propoxy)phenyl]-butanamide with high enantioselectivity

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Science china chemistry 2009年第8期52卷 1216-1219页

作者： WANG NaiXing1,2 & TANG XinLiang1,3 1 Technical institute of Physics and chemistry,Chinese Academy of Sciences,Beijing 100080,china 2 College of chemistry and chemical engineering,Graduate University of Chinese Academy of Sciences,Beijing 100049,china 3 School of chemical engineering and Environment,Beijing institute of Technology,Beijing 100081,china 1. Technical institute of Physics and chemistry Chinese Academy of Sciences Beijing 100080 China 2. College of chemistry and chemical engineering Graduate University of Chinese Academy of Sciences Beijing 100049 China 3. School of chemical engineering and Environment Beijing Institute of Technology Beijing 100081 China

R-Enantiomer of the β-receptor antagonist N-[3-acetyl-4-(2-hydroxy-3-isopropylamino-propoxy)phenyl] butanamide with high enantioselectivity was synthesized from cheap starting materials and enantiopure chiral reagent through an efficient,convenient and practical synthetic *** product was detected by 1H NMR,13C NMR,and MS,and the enatiomeric excess was determined by chiral HPLC analysis using a chiracel AD-H column.

关键词： chiral synthesis enantioselectivity enatiomeric excess chiral HPLC analysis

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Science china chemistry 2004年第6期47卷 466-479页

作者： SONG Huajie1,2, XIAO Heming1 & DONG Haishan2 1. department of chemistry, Nanjing University of Science and Technology, Nanjing 210094, china 2. institute of chemical Materials, china Academy of engineering Physics, Mianyang 621900, china 1. department of chemistry Nanjing University of Science and Technology 210094 Nanjing China 2. institute of chemical Materials China Academy of Engineering Physics 621900 Mianyang China

The symmetry-adapted perturbation theory (SAPT) that has the ability in decompo- sition of the total interaction energy into physically meaningful components is used to provide a more fundamental understanding of intermolecular forces. This work was motivated by the diffi- culty of standard SAPT in computing the intermolecular interactions for large energetic dimer systems. SAPT based on Kohn-Sham orbitals (SAPT(DFT)) proves computationally efficient for these large systems, but has been shown to perform poorly for interaction energy components. The deficiencies of SAPT(DFT) result from wrong asymptotical behaviors of commonly used exchange-correlation potentials. To remove the deficiencies, two asymptotic corrections by means of van Leeuwen and Baerends (LB) model potential and Fermi-Amaldi (FA) type potential were applied into three small test systems comprising He2, HF2 and (N2)2 and a set of larger ni- tramide dimers at several separations. The results showed that when utilizing newly developed frequency-dependent density susceptibilities (FDDS) technique for computing dispersion energy, the FA asymptotic correction is very effective to circumvent these deficiencies in SAPT(DFT) and yields a good accuracy over the LB correction. The FA corrected SAPT(DFT) approach is capa- ble of correctly predicting all the quantitative trends in binding energies for all test cases and substantially reduces computational cost as compared with the standard SAPT calculations. The successful application of the approach to nitramide dimer demonstrates that it potentially pro- vides a good means to calculate accurately intermolecular forces in larger system such as en- ergetic systems.

关键词： intermolecular interaction, density functional theory (DFT), symmetry adapted perturbation theory (SAPT), asymptotic correction, frequency-dependent density susceptibilities (FDDS).

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Efficient degradation of dye pollutants using dioxygen mediated by iron(Ⅱ) 2,2′-bipyridine loaded layered clay catalyst under visible irradiation

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Science china chemistry 2006年第5期49卷 407-410页

作者： CHENG Mingming1,SUN Zhenying1,2,MA Wanhong1,CHEN Chuncheng1,ZHAO Jincai1 & WANG Zhengping2 1. Laboratory of Photochemistry,Center for Molecular Science,institute of chemistry,Chinese Academy of Sciences,Beijing 100080,china 2. department of chemical engineering,Harbin engineering University,Harbin 150001,china 1. Laboratory of Photochemistry Center for Molecular Science Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 2. department of chemical engineering Harbin Engineering University Harbin 150001 China

The organic-inorganic layered solid catalyst Fe(bpy)_3^(2+)-laponite was ableto photode-grade Rhodamine B (RhB) by activation of dioxygen under visible irradiation (λ > 420nm), while the homogeneous aqueous solution Fe(bpy)_3^(2+) showed no photoactivity. The catalystcould be repeatedly used and retained its reactivity. The hybrid catalyst could be separated fromthe suspension by centrifugation or sedimentation. The TOC removal yield of RhB was *** oxygen species (ROS) formed during degradation was detected by EPR. A possible mechanismwas proposed on the basis of the experimental results.

关键词： laponite, Rhodamine B, molecular oxygen, visible light.

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Ion Selective Electrode Array Analysis Using Variable Step-Size Generalized Simulated Annealing

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chemical Research in Chinese Universities 1993年第4期9卷 296-302页

作者： WANG Shi-hua, XIAO Dan and YU Ru-qin (department of chemistry and chemical engineering,hunan Univ. ,Changsha,410082)

A global optimum location algorithm called Variable Step-Size Generalized Simulated Annealing(VSGSA) was applied to treating the data obtained by using an array of ion-electrodes in solutions containing mixtures of Na+, K+, Ca2+. Unlike traditional optimization algorithms such as simplex procedure, VSGSA can be used to determine the model parameters without any priori information about the analytical system under investigation and overcome the disadvantage of simplex method which might converge to local extrema depending on the starting positions. The algorithm was applied to po-tentiometric determination of ions in mixture solutions.

关键词： Chemometrics Multivariate calibration Array of ion-selective electrodes,Generalized simulated annealing

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