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土壤中砷污染研究进展

土壤 2004年第3期36卷 264-270页

作者：蒋成爱吴启堂陈杖榴华南农业大学资源环境学院广州510642 华南农业大学兽医学院广州510642

本文综述了土壤中As污染的主要污染源,土壤中主要含As化合物的形态及转化,土壤对As的吸附缓冲行为,土壤As对植物和微生物的生态毒理及微生物在As不同形态转化中的作用等方面的研究成果;并分析了尚需加强研究的方面。

关键词：土壤砷形态吸附氧化还原微生物转化生态效应

ANALYSIS OF S1 GENE NEPHROPATHOGENIC INFECTIOUS BRONCHITIS VIRUS FIELD ISOLATES

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ANALYSIS OF S1 GENE NEPHROPATHOGENIC INFECTIOUS BRONCHITIS V...

面向21世纪的动物科学与动物医学国际研讨会

作者： Zhang Guiyun Gan Menghou Wang Linchuan Li Wengang Zhengzhou College of Animal Husbandry Engineering Zhengzhou 450008china agricultural University Beijing 100094 South china agricultural University guangzhou 510642

＜正＞Ten field IBV isolates from several provinces in china and three reference strains of infectious Bronchitis Virus（IBV）, used in the study, they were propagated in 10-day-old SPF embryonating chicken eggs and then purified through supercentrifugation. Method of sodium dodecyl sulfate（SDS）-proteinase K-acid phenol was used to extract the genome RNA of IBV. A fragment of 1740 base pairs（bp） which contains the S1 glycoprotein gene of IBV was amplified by RT-PCR, and digested with four different restriction enzymes. The first group includes Hua, 9302 and Lian Yun gang strains, which characteristics of getting no RT-PCR products of S1

关键词： Infectious Bronchitis Virus (IBV) Reverse transcription polymerase chain reaction (RT-PCR) Restriction fragment length polymorphism (RFLP)

High copper levels promotes broiler hepatocyte mitochondrial permeability transition in vivo and in vitro

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High copper levels promotes broiler hepatocyte mitochondrial...

中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Rongsheng Su~(a1),Rongmei Wang~(b1),Huabin Cao~(c1),Jiaqiang Pan~a,Lijun Chen~a, Chengmei Li~a,Dayou Shi~a,Zhaoxin Tang~(a*) (a College of veterinary Medicine,South china agricultural University,guangzhou 510642, china b College of Ying Dong bio-engineering,Shaoguan University,Shaoguan 510042,china c College of Animal Science and Technology Jiangxi agricultural University Nanchang 330045, china)

This study was to examine the effects of copper on the mitochondrial non-specific pore.360, one-day-old,healthy Arbor Acres(AA) broilers were fed with different concentrations(11,110,220 and 330 mg/kg) of copper originated from copper sulfate,tribasic copper chloride(TBCC) or copper *** the indicated time point,the mitochondrial permeability transition(MPT) and copper concentration were analyzed. Results showed that,under the same copper concentration,the MPT of broilers fed copper methionine was the greatest,followed by TBCC,then copper *** effects of copper on MPT were time- and dose-dependent. Furthermore,in vitro in the presence of K,5μM Cu could cause permeability transition as compared to 10μM Cu in buffer without *** these results together,we have shown that hepatocellular MPT may be influenced not only by source and concentration of copper or the raising period of broilers,but also by the existence of K.

关键词： Copper Broiler Mitochondria Mitochondrial permeability transition

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广州地区黑白花奶牛泌乳曲线数学模型的研究

畜牧与兽医 1994年第2期26卷 52-54页

作者：王前林玲林继生广州市奶牛研究所广东省应用气象研究所

本研究根据广州地区黑白花奶牛１１３个完整泌乳期的逐日产奶记录资料，研究了γ函数模型、多项式模型和回归模型的拟合和预报情况。结果表明：上述三个模型对广州地区黑白花奶牛泌乳曲线的拟合效果均不理想。因此，提出一个改进的多项... 详细信息

本研究根据广州地区黑白花奶牛１１３个完整泌乳期的逐日产奶记录资料，研究了γ函数模型、多项式模型和回归模型的拟合和预报情况。结果表明：上述三个模型对广州地区黑白花奶牛泌乳曲线的拟合效果均不理想。因此，提出一个改进的多项式模型：＾ｙ＝ｋ＿０＋ｋ＿１ｔ＋ｋ＿２ｔ￣２＋ｋ＿３ｌｎｔ＋ｋ＿４ｌｎ￣２ｔ＋ｋ＿５ｅ（￣－１３７．５／ｔ）．加ｅ￣（－１３７．５／ｔ）这一项的目的是对奶牛中后期产奶量进行有效的修正。应用此模型有８０％样本拟合相对误差＜１０％，绝对误差＜５００ｋｇ，复相关系数＞０．９，全部样本均达Ｐ＜０．０１显著水平。

关键词：乳牛泌乳黑白花乳牛广州地区

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麋鹿朊蛋白全基因的克隆及序列分析

中国兽医科学 2013年第2期43卷 131-135页

作者：刘雨田孙成友李娟迟田英张鑫鑫于小静王志亮中国动物卫生与流行病学中心山东青岛266032 华南农业大学兽医学院广东广州510642 山东农业大学动物医学院山东泰安271018 青岛农业大学动物科技学院山东青岛266109

为获得中国麋鹿朊蛋白(PRNP)的全基因,并利用DNAStar软件与已报道的其他动物的朊蛋白全基因序列进行同源性分析,根据GenBank中马鹿朊蛋白基因序列设计特异性引物,采用PCR方法从提取的总基因组中扩增得到麋鹿PRNP基因;将其连接到pGEM-T E... 详细信息

为获得中国麋鹿朊蛋白(PRNP)的全基因,并利用DNAStar软件与已报道的其他动物的朊蛋白全基因序列进行同源性分析,根据GenBank中马鹿朊蛋白基因序列设计特异性引物,采用PCR方法从提取的总基因组中扩增得到麋鹿PRNP基因;将其连接到pGEM-T Easy载体中,获得克隆产物并测序。结果,成功扩增出了中国麋鹿PRNP基因,构建了重组质粒pGEM-T-ML;对克隆产物的序列分析表明,麋鹿PRNP基因的开放阅读框包含771bp,编码256个氨基酸的前体蛋白,前体蛋白的分子质量约为28 200u;与已报道的其他鹿科动物朊蛋白全基因的核苷酸及氨基酸序列的同源性均在98.8%以上,与几种非鹿科动物的同源性在96.5%～98.4%之间。结果表明,麋鹿PRNP基因的核苷酸和编码蛋白的氨基酸序列与其他鹿科动物的序列具有高度保守性,而与几种非鹿科动物间的同源性也较高,说明朊蛋白是一种古老的高度保守的蛋白。

关键词：麋鹿朊蛋白全基因克隆序列分析鹿慢性消耗性疾病

In vitro effect of copper chloride exposure on reactive oxygen species generation and respiratory chain complexes activities of mitochondria isolated from broiler liver

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In vitro effect of copper chloride exposure on reactive oxyg...

中国畜牧兽医学会家畜内科学分会第七届代表大会暨学术研讨会

作者： Rongsheng Su~(a1).Rongmei Wang~(b1).Shining Guo~(a1).Huabin Cao~(c1).Jiaqiang Pan~a. Chengmei Li~a.Dayou Shi~a.Zhaoxin Tang~(a*) (a College of veterinary Medicine,South china agricultural University,guangzhou 510642,china b College of Ying Dong bio-engineering,Shaoguan University,Shaoguan 510042,china c College of Animal Science and Technology Jiangxi agricultural University Nanchang 330045,china)

This study is to examine if Cu can act directly on mitochondria or indirectly by producing reactive oxygen species(ROS),isolated broiler hepatic mitochondria were exposed to different concentration of Cu(10,30,50μM). Respiratory chain complexes activities,ROS generation,respiratory control ratio(RCR) and mitochondrial membrane potential(MMP) were ***-dependent inhibition of respiratory chain complexes and induction of ROS were observed,which coincided with decreasing of respiratory control ratio(RCR) both with glutamate+malate or succinate. Further investigation indicated that the membrane potential determined by Rhodamine 123 release decreased after CuCl exposure at 30 and 50μ*** additioa effects of the antioxidants NAC(200μM) and GSH(200μM) were studied at 50μM *** results indicate Cu can induce mitochondrial dysfunction in excessive dose and the effect of Cu exposure on respiratory chain is not site-specific,and antioxidants can protect the mitochondrial function by reducing the formation of free radicals.

关键词： copper chloride(CuCl2) Reactive oxygen species Respiratory chain complexes Free radicals Membrane potential

犬猫蛔虫种特异PCR鉴定方法的研究(英文)

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犬猫蛔虫种特异PCR鉴定方法的研究(英文)

2005年寄生虫学国际学术研讨会暨中国动物学会寄生虫专业委员会第十次学术研讨会

作者： Li Mingwei Chen Honghong Dong Jiawen He Yanyang Lin Ruiqing Zou Fengcai Zhu Xingquan (College of veterinary Medicine, South china agricultural University, guangzhou, guangdong Province 510642, china.Department of Animal Science, agricultural College, Zhanjiang Ocean University, Zhanjiang, guangdong Province 524088, china)

Species of the genera Toxocara and Toxascaris are common parasitic nematodes of dogs and cats, causing significant health problems. Toxocara canis is of known medical significance since its larvae are able to invade human tissues causing diseases such as ocular larva migrans (OLM) and visceral larva migrans (VLM). In the present study, based on the published sequences of the internal transcribed spacers (ITS) of ribosomal DNA (rDNA) of Toxocara canis, T. cati, T. malaysiensis and Toxascaris leonina, four species-specific forward primers were designed in the ITS-1 or ITS-2. With the common conserved reverse primer NC2 and the species-specific forward primers, ITS-1 and ITS-2 of rDNA were respectively amplified by PCR from 99 DNA samples representing four ascaridoid species from dogs and cats from china, Australia, Malaysia, England and Holland for the specific identification of these ascaridoid nematodes. The specificity tests indicated that there was no cross-reaction between these ascaridoids and T. vitulorum, Ascaris suum, Ascaridia galli and Anisakis simplex. The minimum detectable DNA concentrations of the specific assays were 0.14ng/μl for T. canis, 0.54ng/μl for T. cati, 0.38ng/μl for T. malaysiensis and 0.13ng/μl for Tα. Leonina, respectively. The sequencing results of the amplified specific partial ITS1 and ITS-2 fragments confirmed the validity of the specific PCR assays. These specific PCR assays could be applied to distinguish these ascaridoid species, and should provide a useful tool for the diagnosis and the epidemiology investigation of toxocariasis in humans.

关键词： Toxocara canis T. cati T. malaysiensis Toxascaris leonina Specific PCR assay Specificity test Sensitivity test

Rapid detection ofListeria monocytogens by nested-PCR assay

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Rapid detection ofListeria monocytogens by nested-PCR assay

The 15th World veterinary Poultry Congress

作者： Baohua Ma~1 Huihua Zhang~(2 3) Ping Lv~1 Yongchang Cao~2 Yingzuo Bi~2 1 Nanhai Entry-Exit Inspection and Quarantine Bureau Foshan 528200. 2 College of Animal Science South china agricultural University guangzhou 510642. 3 Foshan College of Science & Technology Foshan 528000

＜正＞Listeria monocytogens is one of the most important food-borne pathogen *** to the se- quence data published by Genbank,Two pairs of primers were designed to amplify the iap gene of Listeria monocytogens and a Ne...

关键词：

应用RT-PCR技术检测动物组织中的口蹄疫病毒

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微生物学通报 2002年第4期29卷 9-13页

作者：娄高明杜伟贤杨傲冰周秀蓉谢明权广东省兽医生物技术实验室广州510640

通过对影响RT-PER检出率的条件和试剂进行了筛选,确定了提取FMDV RNA的最佳方法和试剂,优选出RT-PCR反应的试剂和最佳反应条件,建立了检测口蹄疫病毒核酸的RT-PER方法。应用所建立的方法检测送检的鲜牛奶、淋巴结、脊髓、牛鼻咽拭子,结... 详细信息

通过对影响RT-PER检出率的条件和试剂进行了筛选,确定了提取FMDV RNA的最佳方法和试剂,优选出RT-PCR反应的试剂和最佳反应条件,建立了检测口蹄疫病毒核酸的RT-PER方法。应用所建立的方法检测送检的鲜牛奶、淋巴结、脊髓、牛鼻咽拭子,结果阳性率分别为41.4％(24/58)、13.33％(2/15)、20％(1/5)、37.5％(12/32);检测4个屠宰场送检的组织样品40份,结果阳性率为.10％-70％;检测送检的奶粉(1份)、水泡皮(1份)、老鼠(2份)、患儿口腔棉拭子(4份),阳性率均为100％;而检测送检的蟑螂4份,阳性率为0。研究表明检测FMDV的RT-PER技术能准确快速地检测肉类、奶类、分泌物、排泄物的带毒、排毒情况,可用于口蹄疫的诊断和流行病学调查。

关键词： RT-PCR技术检测动物组织口蹄疫病毒