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生物化学与生物物理学报 2001年第6期33卷 629-633页

作者：沈璐郭占云陈衍刘兰英冯佑民吉林大学生命科学院中国科学院上海生命科学研究院生物化学与细胞生物学研究所、分子生物学国家重点实验室中国科学院上海生命科学研究院生物化学与细胞生物学研究所?

为了深入研究胰岛素和胰岛素样生长因子 1(IGF 1)的起源和进化以及结构与功能的关系 ,表达了胰岛素和IGF 1的祖先分子———文昌鱼胰岛素样肽 (ILP)。重组单链ILP的基因用化学方法合成 (从cDNA推测的ILPB结构域的C端与A结构域的N端用Ala... 详细信息

为了深入研究胰岛素和胰岛素样生长因子 1(IGF 1)的起源和进化以及结构与功能的关系 ,表达了胰岛素和IGF 1的祖先分子———文昌鱼胰岛素样肽 (ILP)。重组单链ILP的基因用化学方法合成 (从cDNA推测的ILPB结构域的C端与A结构域的N端用Ala Ala Lys三肽连接起来 ,并将B2 8Arg突变为Lys) ,克隆到表达载体pVT10 2 U中 ,ILP在酿酒酵母中得到有效表达。发酵液经 4步分离纯化 ,得到均一的单链ILP ,经质谱测定分子量和氨基酸组成分析证明表达产物正确。通过Lys C蛋白内切酶处理将重组单链ILP转化成双链形式。虽然双链ILP与人胰岛素受体没有结合活力 ,但圆二色性光谱显示它与胰岛素的结构非常相似。用表达的单链ILP免疫新西兰大白兔 ,获得了高滴度的多克隆抗体。

关键词：胰岛素胰岛素样生长因子1 胰岛素样肽文昌鱼表达

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Identification of Novel MicroRNA Genes from Small RNA Libraries of Orysa sativa and Prediction of Their Targets

Identification of Novel MicroRNA Genes from Small RNA Librar...

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第四届全国RNA进展研讨会

作者： Yang Li Wei Li, Botao Zhao, Chengguo Yao, Wenming Qin, Jiamian Wei, Youxin Jin state key laboratory of molecular biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences. shanghai 200031, People’s Republic of china

MicroRNAs are -22nt small RNAs regulate target genes at post-transcriptional level in plants and animals by mediating translational repression or site-specific cleavage through base pairing with target genes, Numerous miRNA genes have been identified through cDNA cloning and computational predictions in both plants and animals. In this study, we identified 71 miRNA gene families, which represent 813 miRNA genes, in *** through construction of small RNA libraries from typical organs at different developmental stages. Analysis of the fold-back structures of the

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香樟树种子中两种Ⅱ型核糖体失活蛋白凝集素活性的研究

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生物化学与生物物理进展 2004年第7期31卷 650-654页

作者：杨海燕李荣秀刘望夷中国科学院上海生命科学研究院生物化学与细胞生物学研究所中国科学院上海生命科学研究院生物工程研究中心

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖... 详细信息

从香樟树成熟的种子分离到两种新的Ⅱ型核糖体失活蛋白 :新丰毒蛋白和辛纳毒蛋白 .两者A链的分子质量虽然相差近一倍 ,但B链的分子质量相同 .Ⅱ型核糖体失活蛋白的A链是RNAN 糖苷酶 ,B链是凝集素 ,A链进入细胞表现毒性在很大程度上依赖于B链的糖结合活性和特异性 .对辛纳毒蛋白和新丰毒蛋白B链的凝集素活性进行了测定和比较 .红细胞凝集实验显示辛纳毒蛋白和新丰毒蛋白具有相同的细胞凝集活性 ,半抗原抑制实验发现它们都属于半乳糖型核糖体失活蛋白 ,荧光光谱法显示它们的糖结合常数也相同 .

关键词：新丰毒蛋白辛纳毒蛋白凝集素荧光光谱法

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Members of miR-169 family are induced by high salinity and transient inhibit NF-YA transcriptional factors

Members of miR-169 family are induced by high salinity and t...

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华东六省一市生物化学与分子生物学会2008年学术交流会

作者： Botao Zhao~1 Liangfa Ge~2 Ruqiang Liang~1 Wei Li~1 Kangcheng Ruan~1 Hongxuan Lin~2 Youxin Jin~(1,*) ~1 state key laboratory of molecular biology,institute of biochemistry and cell biology,shanghai,200031,and ~2National key laboratory of Plant molecular Genetics,institute of Plant Physiology and Ecology,shanghai, 200032,shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200031,china

MicroRNAs(miRNAs)are endogenously expressed small RNAs with length of～22nt. MicroRNAs silence its target genes at post-transcriptional *** plants,microRNAs play various developmental and physiological roles by cleavaging mRNAs *** and high salinity are the most severe environment abiotic stresses and cause the crop loss all over the *** this study,we identified miR-169g,miP,-169n(o)as high salinity responsive microRNAs in ***-169n and miR169o were in a microRNA cluster with a distance of *** high degree of conservation and close phylogenic distance of the pre-miR-169n and pre-miR-169o indicated that they derived from a tandem duplication evolutional event which occurred very *** existence of an ABRE cis-acting element at the upstream region of miR-169n(o) suggested that miR-169n(o)may be regulated by *** our previous study,we found that the osmotic stress resulted by drought induced miR-169g through DRE ***,we revealed that the osmotic and ionic toxicity stress resulted by high salinity induced miR-169g and miR-169n(o)through both DRE and ABRE *** data showed that the both overlap and distinct responses of miR-169 family to drought and salt *** also showed that these miR-169 members selectively cleaved some of NF-YA genes which are CCAAT-box binding transcription factors and participate in transcriptional regulation of large number ***, we found one or more ath-miR-169 member also induced by high *** indicated that the salt-induction of some miR-169 members was a general property in plants.

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β-Arrestins negatively regulate Toll-like/IL-1-receptor signalling through their functional interaction with TRAF6

β-Arrestins negatively regulate Toll-like/IL-1-receptor sig...

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第五届海峡两岸细胞生物学学术研讨会

作者： Yaya Wang,Yawei Tang, Lin Teng, Yalan Wu & Gang Pei laboratory of molecular cell biology. institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences Graduate School of the chinese academy of sciences, 320 Yue Yang Road, shanghai 200031

The Toll-like receptor (TLR)/interleukin 1 receptor (IL-1R) superfamily is central in detecting microbial infection and eliciting optimised host response. Those two key components of the innate immune system are evolutionarily conserved and subjected to delicate regulation. Tumour necrosis factor receptor-associated factor 6 (TRAF6) is critical to mediate TLR/IL-1R activation of nuclear factor-κB(NF-κB) and activating protein 1 (AP-1),

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Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved 2-DE procedure combined with MALDI-TOF-MS

Proteomic analysis of differential protein expression in a h...

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华东六省一市生物化学与分子生物学会2003年学术交流会

作者： Quan Yuan, Jie An, Ding-gan Liu, Li Sun, Ying-zi Ge, Gen-jun Xu and Fu-kun Zhao ( key laboratory of Proteomics and key laboratory of molecular biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200031, china )

Due to its capability of detecting thousands of proteins simultaneously, two-dimensional (2-D) polyacrylamidegel electrophoresis is now being applied widely in proteomic research. In this paper, the proteomic profiles of a hu-man hepatoma revertant cell line CL1, and its original cell line, SMMC7721, were compared by using improved 2-DE procedure-multi-IPGstrips gels (length≤13cm) run simultaneously on one SDS gel (shorten MSOG meth-od).19 proteins that showed significant difference in expression (Pcells, compared to human hepatoma SMMC7721cells, it was found that the expression levels of some proteins related to tumor suppression, like maspin, were up-

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A Method for MicroRNA Targets Identification Based on RISC Complex Coimmunoprecipitaion

A Method for MicroRNA Targets Identification Based on RISC C...

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2008年全国生物化学与分子生物学学术大会

作者： Yi Shi Bo-tao Zhao Bing Huang Yang Li Wen-min Qin Chen-guo Yao You-xin Jin (state key laboratory of molecular biology,shanghai institute of biochemistry and cell biology,shanghai institutes for biological sciences,chinese academy of sciences.shanghai 200031)

MicroRNAs (miRNAs) are a large class of noncoding RNAs that play a pivotal role in gene regulation Hundreds of miRNAs have been discoved in higher eukaryotes and many of them exhibit different roles in development,dif...

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Proteomics of TGF-β1 induced concomitant apoptosis and EMT in AML12 cells

Proteomics of TGF-β1 induced concomitant apoptosis and EMT ...

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华东六省一市生物化学与分子生物学学会2006年学术交流会

作者： Hong-Yu Tian,Ke-Hua Zhang,Xia Gao,Jian-Feng lin, Gen-Jun Xu,Jian-Guo Song, Fu-Kun Zhao (key laboratory of Proteomics, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai, china laboratory of molecular cell biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences. 320 Yue-Yang Road, shanghai 200031,china College of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018, china)

Introduction Transforming growth factor-beta (TGF-β1) conveys regulatory signals through multiple intracellular pathways, subsequently affecting various cellular functions. Apoptosis and epithelial-to-mesenchymal transition (EMT) have been implicated in a variety of biological processes, such as embryonic development,fibrosis and tumor progression. TGF-β1induces concomitant apoptosis and EMT in some epithelial cells, for example AML12 mouse epithelial cell. However, the mechanism that underlie TGF-β1induced the two different processes remain unclear. Here, we studied the changes in the proteome of AML12 cells in response to TGF-β1 treatment. 40 protein spots, affected by TGF-β1, were selected and identified by MOLDI-TOF/TOF. In these spots, 22 of them were upregulated and 18 of them were downregulated. Among them, there are proteins regulating cytoskeleton organization, energy metabolism, and signal transduction. These results provide a novel perspective for the mechanism of TGF-β signaling pathway in apoptosis and EMT.

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Proteomic analysis of differential protein expression in a human hepatoma revertant cell line by using an improved 2-DE procedure combined with MALDI-TOF-MS

Proteomic analysis of differential protein expression in a h...

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第七届全国酶学学术讨论会

作者： Quan Yuan ,Jie An, Ding-gan Liu, Li Sun, Ying-zi Ge, Ying-ling Huang, Gen-jun Xu and Fu-kun Zhao key laboratory of Proteomics and key laboratory of molecular biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200031, china

Due to its capability of detecting thousands of proteins simultaneously,two-dimensional(2-D)polyacrylamide gel electrophoresis is now being appliedwidely in proteomic *** this paper,the proteomic profiles of a humanhe... 详细信息

关键词： MSOG two-dimensional polyacrylamide gel electrophoresis mass spectrometry NF-IL6 3’ UTR human tumorigenesis-related proteins

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Microarray analysis of microRNA expression in rice

Microarray analysis of microRNA expression in rice

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第四届全国RNA进展研讨会

作者： Botao Zhao, Ruqiang Liang, Wei Li, Yang Li, Chengguo Yao, Wenming Qin, Bing Huang, Youxin Jin state key laboratory of molecular biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, chinese academy of sciences. shanghai 200031, People’s Republic of china

MicroRNAs are a large new class of tiny regulatory RNAs found in nematodes, plants, insects and mammals. They have diverse expression patterns and might regulate various developmental and physiological processes by targeting mRNAs for cleavage , translational repression or un-stabilizing the target mRNA. Although several hundred microRNA genes have been identified, little is known about the regulation and pattern of their expression. Relative expression levels of micro RNAs in different tissues can provide clues about their physiological functions. The short length and uniqueness of each microRNA render many conventional tools ineffective.

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