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2011 4th IEEE International Conference on Computer Science and Information Technology(ICCSIT 2011)

作者： Shuiqin Fang Jun Wang Yao Liang Ning Qiu College of Biological and Chemical Engineering jiangsu University of science and technology zhenjiang 212.18 china Fuan Wu* The sericultural research institute Chinses academy of Agriculture sciences zhenjiang 212.18 china

The fungus train ***1 was isolated from the sick mulberry with root rot, and its pathogenicity was confirmed. Based on the result of its ITS sequence analysis and morphologyyical characters, strain ***1 was identified to be Rhizopus oryzae. The intracellular lipase activity of Rhizopus oryzae was detected by ultrasonication and the result revealed that the intracellular hydrolytic activity of lipase was 11U/ml, the intracellular esterification activity of lipase was 2U/ml. According to the result of enzyme-catalyzed transesterification, Rhizopus oryzae which caused root rot disease of mulberry could catalyze the soybean oil as biodiesel production in three different reaction mixtures. And under the reaction mixtures which were composed of: 9.65 g soybean oil, 0.5 g whole cells, three stepwise addition of methanol（0.35 g each step）, tert-butanol （tert-butanol/oil =1.5:1, v/v）, the methyl yield could reach 44.6%.

关键词： biodiesel lipase Rhizopus oryzae whole cell biocatalyst internal transcribed space(ITS)

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Genetic diversity of local mulberry varieties from different ecotypes revealed by ISSRs in china

Genetic diversity of local mulberry varieties from different...

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江苏省遗传学会植物分子育种学术研讨会

作者： Zhao Weiguo 1,2 Jia Gaofeng 2 Wang Xiaoming 2 Qi Jinliang 2 Pang Yanjun 2 Huang Yongping 3 Pan Yile 1* Yang Yonghua 2* (1 sericultural research institute, chinese academy of agricultural sciences, zhenjiang City jiangsu Province 212.18, china 2 institute of Plant Molecular Biology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life sciences, Nanjing University, Nanjing, jiangsu 210093, china 3 Shanghai institute of Plant Physiology and Ecology, Shanghai institute for Biological sciences, chinese academy of sciences, 300 Fenglin Road, Shanghai 200032, china )

Mulberry is a perennial and economically important plant that has traditionally been used for feeding the silkworm. Evaluating genetic relationship is important for long-term improvement in mulberry yield, quality and resistance, and for germplasm conservation and identification. Population structure and genetic diversity of 8 mulberry populations from different ecotypes in china were analyzed by ISSR markers. 12 SSR primers generated a total of 83 amplification products, of which 50 were polymorphic, revealing 60.24% polymorphism among 66 mulberry local varieties, the mean PIC value was 0.1469. The total heterozygosity （HT）, heterozygosity within population （HS）, diversity between populations （DST） were 0.1600, 0.0851 and 0.0749, respectively. The coefficient of population differentiation （GST） was 0.5678, indicating that the genetic variation between populations was higher than that of within populations. The gene flow （Nm） was 0.4683, suggesting that genetic drift between populations caused local genetic differentiation and therefore, population divergence. The mean genetic similarity coefficient was 0.8456, genetic similarity coefficient among 8 mulberry populations ranged from 0.8441 to 0.9640, indicating that genetic diversity of different populations existed variation. A dendrogram of all 66 local varieties of mulberry based on the genetic similarity using ISSR markers was generated by UPGMA cluster method. In the dendrogram,most varieties from the same ecotype clustered together.

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Genetic diversity between mulberry cultivated and wild species by ISSR and SSR markers analysis

Genetic diversity between mulberry cultivated and wild speci...

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中国蚕学会第四届青年学术研讨会

作者： Zhao Weiguo~(1,3) Zhou Zhihua~2 Wang Sibao~3 Miao Xuexia~3 Pan Yile(?) Huang Yongping~3 ~1sericultural research institute,chinese academy of agricultural sciences,zhenjiang City,jiangsu Province 212.18,china.~2Shanghai Jiao Tong University,Shanghai 200000,china.~3Shanghai institute of Plant Physiology and Ecology,Shanghai institute for Biological sciences,chinese academy of sciences,300 Fenglin Road,Shanghai 200032,china

＜正＞ In this study,inter-simple sequence repeats （ISSR） and simple sequence repeat （SSR）markers were used to investigate genetic diversity of 27 mulberry accessions including 19cultivated accessions （six ***,three ***,two ***,two ***,one ***,two ***,one *** ***,one *** ***,andone *** ***） and 8 wild accessions （two ***,two ***,two ***,one *** and one ***）.ISSRs and SSRs were compared in terms of theirinformativeness and efficiency in a study of genetic diversity and relationships among 27mulberry *** presented a higher level of polymorphism and greater *** index of genetic diversity both markers indicated that within wild species had highergenetic diversity than within cultivated *** mean genetic similarity coefficients betweenmulberry cultivated and wild species ascribed by ISSR and SSR matrices were 0.7677 and 0.6131,*** all markers a high similarity in dendrogram topologies was obtained althoughsome differences were *** analysis of ISSR and SSR using UPGMA methodrevealed that the wild species are genetically distant from the domesticated species studied *** correlation coefficients of similarity were statistically significant for both marker *** coordinates analysis （PCA） for ISSR and SSR data also supports their UPGMAclustering.

关键词： Genetic diversity DNA polymorphism ISSR SSRs Morus L Introduction

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Mulberry genetic diversity of wild populations in northeast china by molecular markers

Mulberry genetic diversity of wild populations in northeast ...

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中国植物学会植物结构与生殖生物学专业委员会、江苏省植物学会2007年学术年会

作者： Wei-Guo Zhao~(1,2) Jian-Qiu Zhang~3 Yu-Huang WANG~1 Ting-Ting Chen~2 Ya-Le Yin~2 Yong-Ping Huang~4 Yi-Le Pan~(1*) Yong-Hua Yang~(2*) 1.sericultural research institute,chinese academy of agricultural sciences,zhenjiang,jiangsu 212.18,china 2.institute of Plant Molecular Biology,State Key Laboratory of Phannaceutical Biotechnology,School of Life sciences,Nanjing University,N anjing,jiangsu 210093,china 3.Forestry academy of Baicheng,Baicheng,Jilin 137000,china 4.Shanghai institute of Plant Physiology and Ecology,Shanghai institute for Biological sciences,chinese academy of sciences,Shanghai 200032,china

Evaluating genetic diversity in mulberry consitutes an indespensible part in the use for taxonomical grouping and utilization in breeding and conservation *** this paper, population structure and genetic diversity of 2 populations of *** from Xianghai National Natural Reserve and Tongfa pastureland in Baicheng city,northeast china's Jilin province,were analyzed by ISSR *** ISSR primers generated a total of 80

关键词： Mulberry Genetic diversity ISSR

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A study on molecular genetic structure of local mulberry varieties in china

A study on molecular genetic structure of local mulberry var...

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中国植物学会植物结构与生殖生物学专业委员会、江苏省植物学会2007年学术年会

作者： Zhao Weiguo~(1.2) Wang Yuhuang~2 Chen Tingting~2 Jia Gaofeng~2 Wang Xiaoming~2 Ya-Le Yin~2 Qie Jinliang~2 Pang Yanjun~2 Han Jin~3 Huang Yongping~4 Pan Yile~(1*) Yang Yonghua~(2*) 1 institute of Plant Molecular Biology,State Key Laboratory of Pharmaceutical Biotechnology,School of Life sciences,Nanjing University,Nanjing,jiangsu 210093,china 2 sericultural research institute,chinese academy of agricultural sciences,zhenjiang City jiangsu Province 212.18,china 3 Hebei Cangzhou Normal College,Hebei,A061001,china 4 Shanghai institute of Plant Physiology and Ecology,Shanghai institute for Biological sciences,chinese academy of sciences,300 Fenglin Road,Shanghai 200032,china

Mulberry is a perennial and economically important plant that has traditionally been used for feeding the *** genetic relationship is important for long term improvement in mulberry yield,quality and resistance,and for germplasm conservation and identification. Population structure and genetic diversity of 8 mulberry populations from different ecorypes in

关键词： Mulberry Local variety Genetic variability ISSR

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Molecular cloning and Expression Patterns of a Calmodulin Gene MCaM-3 From Mulberry (Morus L.)

Molecular cloning and Expression Patterns of a Calmodulin Ge...

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江苏省遗传学会第八届会员代表大会暨学术研讨会

作者： Fang Rong-Jun~(a,b) Hu Dong-Qing~(a,c) Zhao Wei-Guo~(a,c*) Liu Li~a Cheng Jia-Ling~a Qi Jin-Liang~b Yang Yong-Hua~b (a.sericultural research institute,chinese academy of agricultural sciences,zhenjiang jiangsu 212.18,china b School of Life sciences,Nanjing University,Nanjing jiangsu 210093,china c College of biological and Environmental Engineering, jiangsu University of Science and technology,zhenjiang jiangsu 212003,china )

Calmodulin(CaM) is a multifunctional Ca-binding protein in eukaryotic cells,it plays important roles in regulating the growth and development,environment adaptation and stress tolerance in plant. A full-length cDNA sequence coding CaM of mulberry tree,which we designated MCaM-3(GeneBank accession No:GQ303247),was cloned based on the ESTs from mulberry *** sequence analysis showed that the MCaM-3 is 951bp in length,it contains a 143bp 5’-UTR(untranslated region) and a 358bp 3’-UTR,an open reading frame(ORF) of 450bp,encoding 149 amino acids with a predicted molecular weight of 16.85kD and an isoelectric point of *** SMART analysis showed that the protein of MCaM-3 has four EFh functional domains,which can bind *** analysis revealed that,CaM genes are highly conservative in mulberry and other species including Arabidopsis,poplar,soybean,wheat,rice and maize. Phylogenetic analysis based on MCaM-3 and CaM genes in other 19 species showed that mulberry tree shares close relationships with castor oil plant,tobacco and so *** expression level of MCaM-3 at different developmental stages of mulberry leaves and flowers and different sites in mulberry were also investigated,the MCaM-3 transcripts can all be detected with the most abundant expression on mature tissues in mulberry,such as mature female flower and climax *** results of semi-quantitative RT-PCR analysis showed that the expression lever of its mRNA could be increased significantly under the conditions of low temperature,drought and salt stress respectively compared to the normal growth *** results validated the proteins encoded by MCaM-3 may be involved in a general response to abiotic stresses in *** research will help to understand the resistance mechanism of functional genes in mulberry in the future.

关键词： Morus L. MCaM-3 gene Molecular cloning EFh Expression

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HPLC in Reversed Phase Mode:Tool for Investigation of Isoquercitrin from Enzymatic Biosynthesis Products of Rutin

HPLC in Reversed Phase Mode:Tool for Investigation of Isoque...

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2009 International Conference of Natural Products and Traditional Medicine(ICNPTM’ 09)

作者： Jun Wang1,Fu-An Wu1,2 *,Lu-Lu Zhao1,Tao Wu2,Qing Yang3,Yao Liang1,Shui-Qin Fang1,Xing Jiang1,Meng Wang1 1College of Biological and Environmental Engineering,jiangsu University of Science and Technology,zhenjiang 212.18,P.R.china 2sericultural research institute,chinese academy of agricultural sciences,zhenjiang 212.18,P.R.china 3College of Food Science,Southwest University,Chongqing 400715,P.R.china

Isoquercitrin（quercetin 3-o-β-glucoside） can be obtained on a large scale by directional hydrolysis of rutin with hesperidinase,which has more physiologically potential application than rutin in a wide range of biological activities.A rapid and inexpensive HPLC method in reversed phase mode has been developed for the quantitative analysis of rutin and its enzymatic hydrolysis *** in HPLC-UV was on an Alltima C18 column（250mm×4.6mm i.d.;5μm） with a mobile phase consisting acetonitrile:0.02% phosphoric acid solution（20:80 v/v）,at a flow rate of 1.0 mL·min-1,detected at *** equations of rutin and isoquercitrin were y = 0.0000002x-19287（r2 =0.9973） and y= 0.0000002x-4648（r2 =0.9990）,which showed good linear relationships between the peak area of each marker and concentration,*** method has good repeatability and precision,the intraday and interday RSD for both retention time and peak area was less than 1.10%.The mean recoveries of rutin and isoquercitrin in samples,measured at three concentration levels,were 96.6 and 98.9%,their RSD were 2.1% and 1.4 %（n = 3）,*** biotransformation conversion rate of isoquercitrin was the highest when crude hesperidinase was used as *** niger LJ-11 was selected as producer and it was used to obtain the crude hesperidinase in submerged fermentation with added *** results indicated that the developed assay method was rapid,accurate,reliable and could be readily utilized as a quantitative analysis tool for investigation of isoquercitrin from enzymatic biosynthesis products of rutin.

关键词： HPLC isoquercitrin rutin enzymatic biosynthesis

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家蚕核多角体病毒解旋酶基因启动子功能区域缺失分析

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生物化学与生物物理学报 2002年第5期34卷 560-564页

作者：肖庆利张志芳易咏竹何家禄吴祥甫中国农业科学院蚕业研究所、农业部家蚕生物技术重点开放实验室中国科学院上海生命?

杆状病毒DNA解旋酶是病毒复制所必需的。瞬时表达分析显示 ,家蚕核多角体病毒解旋酶基因启动子属于延迟早期基因启动子。通过PCR技术在该启动子区产生的一系列缺失分析表明 ,解旋酶基因启动子的基础转录调控区主要位于ATG上游 - 5 1 0～... 详细信息

杆状病毒DNA解旋酶是病毒复制所必需的。瞬时表达分析显示 ,家蚕核多角体病毒解旋酶基因启动子属于延迟早期基因启动子。通过PCR技术在该启动子区产生的一系列缺失分析表明 ,解旋酶基因启动子的基础转录调控区主要位于ATG上游 - 5 1 0～ - 4 1 0bp之间。当只保留ATG上游 98bp区段时 ,仍可测到该启动子的基础活性。在病毒因子存在下 ,将启动子区域删除到ATG上游 - 4 1 0bp时 ,对启动子活性影响不大 ;若继续删除 ,则其活性显著下降。据此推测对病毒因子响应的启动子区段应主要位于ATG上游 - 4 1 0～ - 30 9bp之间

关键词：家蚕核多角体病毒解旋酶基因启动子瞬时表达缺失分析

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Transcriptional Activity of Drosophila hsp70 Promoter Enhanced by the Homologous Region 3 from Bombyx mori Nucleopolyhedrovirus in Insect Cells

Transcriptional Activity of Drosophila hsp70 Promoter Enhanc...

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中国蚕学会第四届青年学术研讨会

作者： Shun-Ming Tang Yong-Zhu Yi Ya-Jing Zhou Zhi-Fang Zhang Yi-Ren Li Jia-Lu He Key Laboratory of Silkworm Biotechnology,Ministry of Agriculture,sericultural research institute,chinese academy of agricultural sciences,zhenjiang 212.18,china

＜正＞ Drosophila melanogaster heat shock protein 70 gene promoter （Dhsp 70p） is widely used in transgenicinsect to drive exogenous gene,and the homologous region 3 from Bombyx mori nucleopolyhedrovirus（BmNPVhr3） functions as an enhancer for several *** test whether BmNPVhr3 can enhance the Dhsp7Op’s transcriptional activity,the reporter plasmids,which contain the Dhsp70p,thereporter.（?）-galactosidase gene with SV40 terminator and BmNPVhr3 fragment,are constructed and transfectedinto the insect cell lines （Bm-N cells and Sf-21 cells） by lipofectin-mediated *** results from the transientexpression assay show that BmNPVhr3 increases significantly Dhsp70p’s transcriptional activity both under thenormal condition and under the heat-shock treatment,although the effects are significantly different between inBm-N cells and in sf-21 *** enhancement way of BmNPVhr3 on the Dhsp70p is in anorientation-independent ***,the effects of heat-shock treatment on Dhsp70p alone orDhsp70p/BmNPVhr3 combination present no significant difference,indicating that BmNPVhr3 only enhances thetranscriptional activity of Dhsp70p,but can’t alter the characteristic of the response to the heat *** aboveresults suggest that the Dhsp70p/BmNPVhr3 combination is more effective one to drive exogenous gene fortransgene or stable cell expression system in insects.

关键词： Heat shock protein 70 gene Promoter Enhancer Insect cell line BmNPVhr3 Transient expression

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A Cationic Detergent Enhancing the Replication of Baculovirus and the Activity of BmNPV ie-1 Promoter In vivo and In vitro

A Cationic Detergent Enhancing the Replication of Baculoviru...

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中国蚕学会第三届青年学术研讨会暨浙江省第二届青年学术论坛—蚕桑分论坛

作者： YajingZhou, Qingli Xiao, Zhifang Zhang, Jialu He & Yuanxing Zhang( Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, sericultural research institute, chinese academy of agricultural sciences, zhenjiang, 212.18, jiangsu, china State Key Laboratory of Bioreactor Engineering, East china University of Science and Technology, Shanghai 200237,china)

An experimental study was undertaken to quantify the effects of cetyltriethylammoniumbromide(CTAB)on the replication of Bombyx mori nuclear polyhedrosls virus(BmNPV)and the trnsientexpression activity of luciferase gene under the control of BmNPV ie-1 gene promoter in cultured *** results showed that the cell-released virus(CRV)titer rose about 3.7-fold by adding CTATAB to culturemedia up to 0.1μg ml while the polyhedra insiae the nucleus of cultured cells dropped linearly within arange of CTAB concentrations from 0 to 0.25μg ml when BmN cells were infected with a ***,the CRV titer was increased by approximate 2-fold in the presence of 0.05μg ml ofCTAB while the expression level ofβ-glactosidase was decreased linearly within this experimental rangeof CTAB concentrations by infection with a polyhedrin-negative recombinant BmNPV(rBmNPV)containing β-glactosidase reporter gene under the control of the polyhedrin gene promoter. Whensilkworm larvae at fifth instar were infected with a rBmNPV contalning phytase gene driven by polyhedrinpromoter,the expression level of phytace had no significant changes by feeding CTAB spread onto themulberry leaves to ***,the expression level of phytase dropped drastlcally by directlyinjecting CTAB into the *** level of transient expression of luciferase under the control of the ie-1gene promoter was given more than a 3-fold increase through treating different cell lines with 0.1μg ml(-1) ofCTAB.

关键词： BmNPV CTAB infection insect cells silkworm larvae transfection

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