Genetic diversity between mulberry cultivated and wild species by ISSR and SSR markers analysis

作     者：Zhao Weiguo~(1,3) Zhou Zhihua~2 Wang Sibao~3 Miao Xuexia~3 Pan Yile(?) Huang Yongping~3 ~1Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang City,Jiangsu Province 212018,China.~2Shanghai Jiao Tong University,Shanghai 200000,China.~3Shanghai Institute of Plant Physiology and Ecology,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences,300 Fenglin Road,Shanghai 200032,China 

会议名称：《中国蚕学会第四届青年学术研讨会》

会议日期：2004年

学科分类：090504[农学-特种经济动物饲养（含：蚕、蜂等）] 0905[农学-畜牧学] 09[农学] 

关 键 词：Genetic diversity DNA polymorphism ISSR SSRs Morus L Introduction 

摘      要：正 In this study,inter-simple sequence repeats （ISSR） and simple sequence repeat （SSR）markers were used to investigate genetic diversity of 27 mulberry accessions including 19cultivated accessions （six ***,three ***,two ***,two ***,one ***,two ***,one *** ***,one *** ***,andone *** ***） and 8 wild accessions （two ***,two ***,two ***,one *** and one ***）.ISSRs and SSRs were compared in terms of theirinformativeness and efficiency in a study of genetic diversity and relationships among 27mulberry *** presented a higher level of polymorphism and greater *** index of genetic diversity both markers indicated that within wild species had highergenetic diversity than within cultivated *** mean genetic similarity coefficients betweenmulberry cultivated and wild species ascribed by ISSR and SSR matrices were 0.7677 and 0.6131,*** all markers a high similarity in dendrogram topologies was obtained althoughsome differences were *** analysis of ISSR and SSR using UPGMA methodrevealed that the wild species are genetically distant from the domesticated species studied *** correlation coefficients of similarity were statistically significant for both marker *** coordinates analysis （PCA） for ISSR and SSR data also supports their UPGMAclustering.