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Expression and Comparative Analysis of Genes Encoding Outer Membrane Proteins LipL21,LipL32 and OmpL1 in Epidemic Leptospires

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Acta Biochimica et Biophysica Sinica 2005年第10期37卷 7+650-656页

作者： Xiang-Yan ZHANG~(1#) Yang YU~(2,3#) Ping HE~1 Yi-Xuan ZHANG~(2,3) Bao-Yu HU~1 Yang YANG~1 Yi-Xin NIE~4 Xiu-Gao JIANG~4 Guo-Ping ZHAO~(2,3) Xiao-Kui GUO~(1*) 1 Department of Medical Microbiology and Parasitology,Shanghai Jiaotong University,Shanghai 200025,china 2 research center of Biotechnology,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,china 3 Chinese National Human Genome center at Shanghai,Zhangjiang High Tech Park,Shanghai 201203,china 4 institute for Infectious Disease control and Prevention,Chinese center for Disease control and Prevention,Beijing 102206,china Department of Medical Microbiology and Parasitology Shanghai Jiaotong UniversityShanghai 200025 China research center of Biotechnology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China Chinese National Human Genome center at Shanghai Zhangjiang High Tech ParkShanghai 201203 China institute for Infectious Disease control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206 China

Leptospiral outer membrane proteins (OMPs) are highly conserved in different species,andplay an essential role in the development of new immunoprotection and serodiagnosis *** genesencoding LipL21,LipL32 and OmpL1 were cloned from the complete genome sequence of Leptospirainterrogans serovar lai strain Lai and expressed in *** comparison analysis revealed that the threegenes were highly conserved among distinct epidemic leptospires,including three major epidemic speciesLeptospira interrogans,Leptospira borgpetersenii and Leptospira weilii,in *** analysis wasfurther performed to scrutinize 15 epidemic Leptospira reference strains using the antisera of the *** immunoblot assay and reverse transcription-polymerase chain reaction demonstrated that thesethree OMPs were conservatively expressed in pathogenic *** strains and other pathogenic ***,the use of these recombinant OMPs as antigens in enzyme-linked immunosorbent assay (ELISA)for serodiagnosis of leptospirosis was *** recombinant LipL32 and OmpL1 proteins showed ahigh degree of ELISA reactivity with sera from patients infected with *** strain Lai and otherpathogenic *** results may contribute to the identification of candidates for broad-rangevaccines and immunodiagnostic antigens in further research.

关键词： outer membrane protein expression comparative analysis epidemic leptospire

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Precision Gravity center Position Measurement System for Heavy Vehicles

Precision Gravity Center Position Measurement System for Hea...

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亚洲精密工程、微纳技术国际会议

作者： Zhao Xintong, Jiang Hongzhou, Zheng Shutao, Han Junwei harbin institute of technology, Harhin, 150001,china

Knowledge of a vehicle's inertial parameters is essential for safety research and accident reconstruction. A precision measure system is proposed to determine the weight and gravity center for heavy vehicles. Based on a static gravity measuring principle with three measuring points, a hydraulically driven 2-DOF motion platform is developed. The transfer function model is derived for the hydraulically driven system. By means of a degree-of-freedom control scheme, the platform can realize accurate positioning to construct two intersected planes and work out the three-dimensional coordinates of the vehicle gravity center. Experiments demonstrate that the system has less than 0.3% measurement error in weight, and is able to measure the gravity centre accurately with deviation ≤3mm in X and Y direction, and ≤5mm in Z direction.

关键词： Vehicle, Weighting, Gravity center, Measurement

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非线性时间序列高性能仿真算法研究

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系统仿真学报 2009年第15期21卷 4598-4602页

作者：邵晨曦童松桃杨明王子才哈尔滨工业大学控制与仿真中心哈尔滨150001 中国科学技术大学计算机科学与技术系合肥230027

系统相空间重构和几何特征值计算是非线性动力系统仿真的重要过程。因其算法时空复杂度较高而计算耗时,为实时仿真非线性时间序列带来了困难。对相图重构算法和几何特征值计算算法进行了优化,使其时间复杂度有不同程度的降低;进而并行... 详细信息

系统相空间重构和几何特征值计算是非线性动力系统仿真的重要过程。因其算法时空复杂度较高而计算耗时,为实时仿真非线性时间序列带来了困难。对相图重构算法和几何特征值计算算法进行了优化,使其时间复杂度有不同程度的降低;进而并行化这些算法,使其效率进一步提升,最后算法运行时间缩短为原运行时间的1/30。以长程睡眠脑电数据分析和仿真为例,分析结果表明关联维的高低能够反映出睡眠的阶段,据此对非快速眼动睡眠期的仿真预测与实际结果相符。

关键词：非线性时间序列仿真算法算法优化并行算法睡眠脑电

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Global view of Hopf bifurcations of a van der Pol oscillator with delayed state feedback

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Science china(Technological Sciences) 2010年第3期53卷 595-607页

作者： ZHANG Li1,WANG HuaiLei1 & HU HaiYan1,2 1 MOE Key Lab of Mechanics and control of Aerospace Structures,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,china 2 School of Aerospace Engineering,Beijing institute of technology,Beijing 100081,china MOE Key Lab of Mechanics and control of Aerospace Structures Nanjing University of Aeronautics and Astronautics School of Aerospace Engineering Beijing Institute of Technology

This paper presents both analytical and numerical studies on the global view of Hopf bifurcations of a van der Pol oscillator with delayed state *** on a detailed analysis of the stability switches of the trivial equilibrium of the system,the stability charts are given in a parameter space consisting of the time delay and the feedback *** center manifold reduc-tion and the normal form method are used to study Hopf bifurcations with respect to the time *** gain an insight into the persistence of a Hopf bifurcation as the time delay varies farther away from its critical value,the method of multiple scales is used to obtain the global view of Hopf bifurcations with respect to the time *** the analytical results of Hopf bifurca-tions and global view of those bifurcations are validated via a collocation scheme implemented on *** most important discovery in this paper is the well-structured global view of Hopf bifurcations for the system of concern,showing the generality of the persistence of Hopf bifurcations.

关键词： Hopf bifurcation global view center manifold reduction method of multiple scales collocation scheme

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Nonlinear H∞ Normal Matrix Attitude control of Spacecraft

Nonlinear H∞ Normal Matrix Attitude Control of Spacecraft

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2009中国控制与决策会议

作者： CUI Ping-Yuan~(1,2),ZHU Hong-Yu~1 1.Deep Space Exploration research center,harbin institute of technology,harbin 150080,Heilongjiang,china 2.School of Astronautics,Beijing institute of technology,Beijing 100081,china

The attitude control problem for rigid spacecraft with disturbances is addressed.A feedback control law for rigid spacecraft attitude was proposed by combining the normal matrix design approach for robust control systems,the feedback perturbation description for systems uncertainties,and the Hamilton-Jacobi-Isaacs partial differential inequality for nonlinear H *** control law is built based on the normal matrix control theory,and the tuning guidelines are established by using the Hamilton-Jacobi-Isaacs *** control law is H solution for the linearized attitude dynamics and nonlinear H solution for the nonlinear attitude motion of attitude tracking ***, no additional assumption about the inertia of spacecraft is needed in the designing *** simulation are performed to demonstrate the effectiveness of the control law proposed.

关键词： Spacecraft Attitude control Robustness Hamilton-Jacobi-Isaacs partial differential inequality Normal matrix Feedback perturbation

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Robust Stability of Genetic Regulatory Networks with Interval Time-varying Delays Under Intrinsic and Extrinsic Noises

Robust Stability of Genetic Regulatory Networks with Interva...

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第二十九届中国控制会议

作者： PAN Wei1,WANG Zidong2,HU Jun3,GAO Huijun3 1.Department of Electronic Science and technology,University of Science and technology of china,Hefei 230027,P.R.china 2.Department of Information Systems and Computing,Brunel University,Uxbridge,Middlesex,UB8 3PH,United Kingdom3.Space control and inertial technology research center,harbin institute of technology,harbin 150001,P.R.china

Gene regulation is inherently a stochastic process due to intrinsic and extrinsic noises which cause the fluctuations and uncertainties of kinetic *** the other hand,time delays are usually inevitable due to different biochemical reactions in the genetic regulatory networks（GRNs）which are also affected by ***,in this paper,we propose a GRN model that is subject to additive and multiplicative noises as well as time-varying *** time-varying delay is assumed to belong to an interval and no restriction on the derivative of the time-varying delay is needed,which allows the delay to be a fast time-varying *** stochastic stability of such GRNs with disturbance attenuation is analyzed by applying the control theory and mathematical *** on the Lyapunov method,new stability conditions are derived in the form of linear matrix inequalities（LMIs）that are dependent on the upper and lower bounds of time *** example is employed to illustrate the applicability and usefulness of the developed theoretical results.

关键词： Robust stability Genetic regulatory network noise Lyapunov-Krasovskii functional Interval time-varying delay

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Optimal Filtering for Delay System with Multiplicative Noise

Optimal Filtering for Delay System with Multiplicative Noise

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第25届中国控制会议

作者： Lu Xiao, Zhang Huanshui, Wang Wei , Wang Wei research center of Information and control, Dalian University of technology, Dalian 116023, china Shenzhen Graduate School, harbin institute of technology, Shenzhen 518055, china

＜正＞The paper deals with the Kalman filtering problem for linear discrete-time systems with parametric uncertainty of multiplicative noise in both instantaneous and delayed measurements. Although the problem may be addressed by applying an existing Kalman filtering technique to an augmented system, the computation of the Kalman filter could be demanding. To improve the efficiency of computation, we propose a new approach through the re-organization of innovation. The calculation of the optimal filter involves two Riccati equations associated with the Kalman filter of a delay-free system and the instantaneous measurement, respectively. The Riccati equations have the same dimension as the system.

关键词： Multiplicative noise Innovation Delayed measurements

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Safety Protection control for a Class of Linear Systems via Switching Strategy

Safety Protection Control for a Class of Linear Systems via ...

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第三十届中国控制会议

作者： CHEN Chao~1,YU Daren~2,BAO Wen~2,ZHAO Jun~1 1.State Key Laboratory of Integrated Automation for Process Industries,Northeastern University, Shenyang 110819,P.R.china 2.harbin institute of technology,Hypersonic technology research center,harbin 150001,P.R.china

＜正＞For a class of linear systems,which can be considered as the simplified hypersonic air-breathing propulsion model,focusing on the safe boundaries existing during the working progressing,this paper studies the output regulation/ safety protection switching control *** remarks are given to the switching control based on minimum switching law studied in our previous work,and to make the designing of the switching controller more systematic,a novel reference command switching strategy is proposed,where a discontinuous controller is adapted,and the asymptotic tracking problem is transformed into the asymptotic stability problem of the linear switched and impulsive *** stability of the switching control system is illustrated,and the principle guaranteeing asymptotic tracking is ***,impacts of switching condition and size of jumping step on the response performances are studied through simulation and it is pointed out that reference commands switching can improve both overshooting and setting time,the ability of tracking commands safely and quickly is *** addition,the design of PI controller and the switching law can be completed separately, and the switching control law is utility.

关键词： Hypersonic Air-Breathing Propulsion Safety Protection Command Switching control Discontinuous controller Tracking Linear Switched and Impulsive System

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Construction of Prophylactic Human Papillomavirus Type 16 L1 Capsid Protein Vaccine Delivered by Live Attenuated Shigella flexneri Strain sh42

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Acta Biochimica et Biophysica Sinica 2005年第11期37卷 743-750页

作者： Xiao-Feng YANG Xin-Zhong QU Kai WANG Jin ZHENG L(?)-Sheng SI Xiao-Ping DONG Yi-Li WANG Key Laboratory of Biomedical Information Engineering of Ministry of Education,institute for Cancer research,Xi'an Jiaotong University,Xi'an 710061.china National institute for Viral Disease control and Prevention,Chinese center for Disease control and Prevention,Beijing 100052,china Department of Obstetrics and Gynecology,First Hospital of Xi'an Jiaotong University,Xi'an 710061,china Key Laboratory of Biomedical Information Engineering of Ministry of Education Institute for Cancer Research Xi'an Jiaotong UniversityXi'an 710061 China Department of Obstetrics and Gynecology First Hospital of Xi'an Jiaotong UniversityXi'an 710061 China National institute for Viral Disease control and Prevention Chinese Center for Disease Control and PreventionBeijing 100052 China

To express human papillomavirus(HPV)L1 capsid protein in the recombinant strain of Shigellaand study the potential of a live attenuated Shigella-based HPV prophylactic vaccine in preventing HPVinfection,the icsA/virG fragment of Shigella-based prokaryotic expression plasmid pHS3199 was *** type 16 L1(HPV16L1)gene was inserted into plasmid pHS3199 to form the pHS3199-HPV16L1construct,and pHS3199-HPV16L1 was electroporated into a live attenuated Shigella strain *** analysis showed that HPV16L1 could be expressed stably in the recombinant strain *** test results were negative,which showed that the sh42-HPV16L1 lost ***,the at-tenuated recombinant strain partially maintained the invasive property as indicated by the HeLa cell *** IgG,IgA antibody against HPV16L1 virus-like particles(VLPs)were detected in the sera,intestinal lavage and vaginal lavage from animals immunized by *** number of antibody-secreting cells in the spleen and draining lymph nodes were increased significantly compared with the *** from immunized animals inhibited murine hemagglutination induced by HPV16L1 VLPs,whichindicated that the candidate vaccine could stimulate an efficient immune response in guinea pig's *** may be an effective strategy for the development of an HPV prophylactic oral vaccine.

关键词： human papillomavirus type 16 attenuated Shigella flexneri vaccine cervical cancer prophylactic

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Corrosion and electrochemical behavior of as-cast Mg-5Y-7Gd-1Nd-0.5Zr magnesium alloys in 5%NaCl aqueous solution

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Progress in Natural Science:Materials International 2011年第4期21卷 314-321页

作者： Xin ZHANG~(1,2,3),Kui ZHANG~1,Xing-gang LI~1,Cong WANG~4,Hong-wei LI~(2,3),Chang-shun WANG~(2,3),Xia DENG~1 1.State Key Laboratory for Fabrication and Processing of Nonferrous Metals, Beijing General research institute for Nonferrous Metals,Beijing 100088,china 2.Beijing Engineering research center for Advanced Manufacturing and Evaluation of Special Vehicle Parts,Beijing 100072,china 3.institute of technology,Beijing North Vehicle Group Corporation,Beijing 100072,china 4.Fengtai Military Representative Office,Beijing Military Representative Office,PLA,Beijing 100072,china State Key Laboratory for Fabrication and Processing of Nonferrous Metals Beijing General Research Institute for Nonferrous Metals Beijing 100088 China Beijing Engineering research center for Advanced Manufacturing and Evaluation of Special Vehicle Parts Beijing 100072 China institute of technology Beijing North Vehicle Group Corporation Beijing 100072 China Fengtai Military Representative Office Beijing Military Representative Office PLA Beijing 100072 China

The corrosion mechanism of the as-cast Mg-5Y-7Gd-1Nd-0.5Zr magnesium alloy immersed in 5%NaCl aqueous solution was investigated by electrochemical test and *** electrochemical results indicate that the corrosion resistance of Mg-5Y-7Gd-1Nd-0.5Zr alloy in 5%NaCl aqueous solution gradually deteriorates with immersion time increasing from 2 h,24 h,60 h,to 108 h,which is consistent with the SEM observation of corrosion *** Cl-anion leads to the initiation and development of the corrosion pits,and Mg-5Y-7Gd-1Nd-0.5Zr alloy exhibits filiform type of attack under significant anodic control of magnesium solution *** corrosion potential becomes noble with increasing immersion time.

关键词： magnesium alloys Mg-5Y-7Gd-1Nd-0.5Zr corrosion electrochemical behavior

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