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Selenocysteine tRNAs as Central Components of Selenoprotein Biosynthesis in Eukaryotes

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Biomedical and Environmental Sciences 1997年第2期10卷 116-124页

作者： SANG ICK PARK JIN Mo PARK HAROLD S. CHITTUM EUN SUNG YANG BRADLEY A. CARLSON BYEONG JAE LEE AND DOLPH L. HATFIELD(Laboratory of Experimental Chrcinogenesis, national Cancer insti tute, national institutes of Health, Bethesda, MD 20892USA Laboratory o Laboratory of Experimental Carcinogenesis National Cancer Institute National Institutes of Health Bethesda MD 20892 USA.

Selenocysteine (Sec) tRNAs serve as carrier molecules for the biosynthesis of Sec from serine and to donate Sec to protein in response to specific UGA codons. In this study, we describe the current status of Sec tRNAs in higher animals and further we exarnine: (i) the Sec tRNA population in Drosophila; (ii) transcription of the Sec tRNA in vivo (in Xenopus oocytes) and in vitro (in Xenopus oocyte extracts); (iii) the effect of selenium on the Sec tRNA population in various rat tissues following replenishment of extremely selenium deficient rats with this element; and (iv) the biosynthesis of the modified bases on Sec tRNA in Xenopus oocytes

关键词： tRNA gene Selenocysteine tRNAs as Central Components of Selenoprotein Biosynthesis in Eukaryotes

The release of ^(14)C-chlorsulfuron bound residue by autoclaving method and its identification

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Nuclear Science and Techniques 1999年第2期10卷 105-110页

作者： GUO Jiangfeng SUN Jinhe PING Xiaofei YE Qingfu(instiftrte of Nuclear Agricultural Science, Zhejiang University (Huajiachi campus),Key Las. of Nuclear Agricultural Science, the Ministry of Agriculture, Hangzhou 310029, China national Rice Research insti institute of Nuclear Agricultural Science Zhejiang University (Huajiachi Campus) Ministry of Agriculture Hangzhou 310029 China China national Rice Research institute Hangzhou 310006 China

When individual microorganisms were used to release bound pesticide residues, it was ussually not take into full account whether the autoclavingmethod could lead to the release of bound residues. The soil containing bound 14Cchlorsulfuron residues was treated with an autoclave in this study. The results revealthat the bound 14C-chlorsulfuron residue can be released from the soil when treatedwith the autoclave and the release rate can be remarkably enhanced by adding water into the soil when subjected to such treatment. A TLC analysis showed that thereleased 14C-residue was one of the degraded products of 14C-chlorsulfuron. Afterderivatization and analysis using the GC-MS, the released 14C-residue was tentativelyidentified aS 2-amino-4-hydroXyl-6-methyl-1,3,

关键词：土壤农药残留量释放

A POTENTIAL REDUCTION ALGORITHM FOR MONOTONE VARIATIONAL INEQUALITY PROBLEMS

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Systems Science and Mathematical Sciences 2000年第1期13卷 59-66页

作者： LIANG Xi ming (institute of Industrial Process Control, national Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310027, China) XU Cheng xian (School of Science, Xi’an Jiaotong University, Xi’an 710049, China) HU Jian bo (insti

***"andletF:R"-R"***(VIP)istofindapointx*eKsuchthatThevariationalinequalityproblemmakesitswideappearanceinthestudyofvariousequilibriummodelsineconomics,oper... 详细信息

***"andletF:R"-R"***(VIP)istofindapointx*eKsuchthatThevariationalinequalityproblemmakesitswideappearanceinthestudyofvariousequilibriummodelsineconomics,oper...

关键词： Variational inequality problems interior point methods potential reduction global convergence.

Analysis of tandem repeats in the genome of Chinese shrimp Fenneropenaeus chinensis

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Chinese Science Bulletin 2005年第14期50卷 1462-1469页

作者： KONG Jie1 & GAO Huan2,3 1. Key Laboratory for Sustainable Utilization of Marine Fisheries Re- sources, Ministry of Agriculture, Yellow Sea Fisheris Research insti- tute, Chinese Academy of Fishery Sciences, Qingdao 266071, China 2. institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China 3. The Graduate School of the Chinese Academy of Sciences, Beijing 100039, China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of Agriculture Yellow Sea Fisheris Research Institute Chinese Academy of Fishery Sciences Qingdao China institute of Oceanology Chinese Academy of Sciences Qingdao China The Graduate School of the Chinese Academy of Sciences Beijing China

Through random sequencing, we found a total of 884000 base-pairs (bp) of random genomic sequences in the genome of Chinese shrimp (Fenneropenaeus chinensis). Using bio-soft Tandem Repeat Finder (TRF) software, 2159 tandem repeats were found, in which there were 1714 mi- crosatellites and 445 minisatellites, accounting for 79.4% and 20.6% of repeat sequences, respectively. The cumulative length of repeat sequences was found to be 116685 bp, ac- counting for 13.2% of the total DNA sequence; the cumula- tive length of microsatellites occupied 9.78% of the total DNA sequence, and that of minisatellites occupied 3.42%. In decreasing order, the 20 most abundant repeat sequence classes were as follows: AT (557), AC (471), AG (274), AAT (92), A (56), AAG (28), ATC (27), ATAG (27), AGG (18), ACT (15), C (11), AAC (11), ACAT (11), CAGA (10), AGAA (9), AGGG (7), CAAA (7), CGCA (6), ATAA (6), AGAGAA (6). Dinucleotide repeats, not only in the aspect of the number, but also in cumulative length, were the preponderant repeat type. There were few classes and low copy numbers of repeat units of the pentanucleotide repeat type, which included only three classes: AGAGA, GAGGC and AAAGA. The classes and copy numbers of heptanucleotide, eleven-nucleotide and thirteen-nucleotide primer-number-composed repeats were distinctly less than that of repeat types beside them.

关键词：虾遗传特性遗传基因 DNA

GENETIC DIVERSITY AMONG CHINESE SIKA DEER (CERVUS NIPPON) POPULATIONS AND RELATIONSHIPS BETWEEN CHINESE AND JAPANESE SIKA DEER

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Chinese Science Bulletin 2006年第4期51卷 433-440页

作者： Lü Xiaoping WEI Fuwen LIMing YANG Guang LIU Hai Key Laboratory for Animal Ecology and Conservation Biology Insti-tute of Zoology Chinese Academy of Sciences Beijing 100080China Graduate School of the Chinese Academy of Sciences Beijing 100039China institute of Genetic Resources Nanjing Normal University Nanjing 210097 China

Sika deer (Cervus nippon) is a cervid endemic to mainland and insular Asia and endan-gered. We analyzed variation in the mitochondrial DNA (mtDNA) control region for four subspecies to understand the genetic diversity, population structure and evolutionary history in China. 335 bp were se-quenced and eight haplotypes were identified based on 25 variable sites among the populations. Sika deer in China showed lower genetic diversity, sug-gesting a small effective population size due to habi-tat fragmentation, a low number of founder individu-als, or the narrow breeding program. AMOVA analy-sis indicated that there was significant genetic subdi-vision among the four populations, but no correlation between the genetic and geographic distance. Phy-logenetic analyses also revealed that Chinese sika deer may be divided into three genetic clades, but the genetic structure among Chinese populations was inconsistent with subspecies designations and pre-sent geographic distribution. Including the sequence data of Japanese sika deer, the results indicated that Chinese populations were more closely related to Southern Japanese populations than to the Northern Japanese one, and the Taiwan population was closer to populations of Northeastern China and Sichuan than to those of Southern China.

关键词：中国梅花鹿遗传多样性衔接重复数量日本梅花鹿

A conserved structural motif shared by EIAV gp90 and HIV gp120

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Science Bulletin 2005年第20期 139-140页

作者： LI Huiguang1, TONG Xiao1,2, SHEN Tao1, SHEN Rongxian3, ZHANG Xiaoyan1 & SHAO Yiming1 1. Department of Research on Virology and Immunology, national Cen- ter for AIDS/STD Control and Prevention, CDC, Beijing 100052, China 2. Wuhan institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China 3. Department of Research on EIAV, Harbin Veterinary Research insti- tute, Harbin 150001, China

Both HIV and EIAV belong to the retroviridae family and lentivirus genus. They share considerable simi- larity at gene structural and component level. Hotzel pro- posed that two variable regions and surrounding amino acids of EIAV gp90 might adopt the same topology as V1, V2 and bridging sheet of HIV gp120. These regions map to V3, V4 and surrounding amino acids of EIAV gp90. Based on this proposal, we further analyzed the distribution profile of N-glycosylation site and linear epitope for EIAV gp90 and HIV gp120 at these regions. Our results demonstrated that EIAV gp90 and HIV gp120 are highly similar at these two aspects. Most likely, V3, V4 and surrounding amino acids of EIAV gp90 adopt the same topology structure as V1, V2 and bridging sheet of HIV gp120.

关键词： EIAV HIV N-glycosylation epitope gp120 gp90.

Effect of 7-hydroxystaurosporine on glioblastoma cell invasion and migration

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Acta Pharmacologica Sinica 2005年第4期26卷 492-499页

作者： Qing-hui MENG~2 Li-xin ZHOU~3 Jia-lin LUO~4 Jian-ping CAO~4 Jian TONG~4 Sai-jun FAN~(2,4,5) 2 Department of Oncology, Lombardi Comprehensive Caneer Center,Georgetown University,Washington DC 20057,USA 3 institute of Medicine and Biotechnology,Chinese Academy of Medical Sciences,Beijing 100050,China 4 School of Radiation Medicine and Public Health,Soochow University,Suzhou 215007,China Department of Oncology Lombardi Comprehensive Cancer Center Georgetown University Washington DC USA institute of Medicine and Biotechnology Chinese Academy of Medical Sciences Beijing China School of Radiation Medicine and Public Health Soochow University Suzhou China

Aim:To investigate the effect of 7-hydroxystaurosporine（UCN-01）,a selectiveprotein kinase C（PKC）inhibitor,on cell growth,migration,and invasion in inva-sive human glioblastoma U-87MG ***:PKC activity was determinedbased on the PKC-catalyzed transfer of the32p-phosphate group from[g-32p]ATPinto a PKC-specific peptide *** viability was measured by MTT *** invasion and migration were evaluated by a Boyden chamber assay andscratch wound assay,*** expression was analyzed using West-ern blot *** formation of 3-dimensional cellular aggregates was examinedby a cell-cell aggregation ***:UCN-01 treatment resulted in concen-tration-and time-dependent inhibition of U-87MG cell growth at higher doses（>100 nmol/L）,and reduced cell invasion and migration capability at less cytotoxicdoses（2-myristate-13-acetate（PMA）and ethanol（EtOH）,2 PKC *** expression ofthe tumor suppressor genes BRCA1 and PTEN increased the anti-invasion poten-tial of *** to UCN-01 caused a dose-dependent increase in celladhesion molecule *** effect of UCN-01 on the formation of cell-cellaggregation was significantly reduced by the addition of an ***:UCN-01 inhibits the invasion and migration of humanglioma ***,UCN-01 can have potential clinical applications for thetreatment of human glioma metastasis.

关键词： 7-hydroxystaurosporine protein kinase C cell movement cadherins BRCA1 protein

辽东湾表层沉积物的重金属污染特征与质量评价

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海洋学报 2018年第6期40卷 60-73页

作者：蓝先洪孟祥君梅西徐扬黄龙陈珊珊青岛海洋地质研究所国土资源部海洋环境与油气地质重点实验室山东青岛266071 青岛海洋科学与技术国家实验室海洋矿产资源评价与探测技术功能实验室山东青岛266071

本文研究了辽东湾表层沉积物中重金属的分布及污染特征,评价其生态风险及环境质量。对辽东湾表层沉积物8种重金属(As、Cu、Cd、Cr、Hg、Ni、Pb、Zn)的空间分布进行了研究,采用Hankanson法和Igeo(地累积指数)分析了该海域重金属潜在生态... 详细信息

本文研究了辽东湾表层沉积物中重金属的分布及污染特征,评价其生态风险及环境质量。对辽东湾表层沉积物8种重金属(As、Cu、Cd、Cr、Hg、Ni、Pb、Zn)的空间分布进行了研究,采用Hankanson法和Igeo(地累积指数)分析了该海域重金属潜在生态风险,并评价了该海域的环境质量。在辽东湾葫芦岛附近海域、西南部六股河口东南部海域以及西部近岸海域表层沉积物中As、Cu、Cd、Cr、Hg、Ni、Pb和Zn含量偏高。地累积指数法及生态风险指数法对重金属污染程度评价结果一致,辽东湾生态环境具有潜在危害的重金属主要是Cd、Hg和As,8种重金属潜在生态风险系数由高到低依次为Cd、Hg、Cu、Pb、As、Zn、Ni和Cr,各站位重金属综合潜在生态风险指数均大于150,生态风险总体上处于中等生态风险等级。Cd和Hg是该海域沉积物重金属中主要污染物,局部区域达到中、中-强污染程度。环境质量评价表明,辽东湾表层沉积物重金属引发有害生物效应的可能性不大,但Cd和Hg显著富集,应予以重视。

关键词：辽东湾表层沉积物重金属质量评价

Fokker-Planck方程局域谐振方法近似解的研究

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高能物理与核物理 1989年第9期13卷 833-836页

作者：孙喆民吴锡真冯仁发卓益忠邢静如张树发中国原子能科学研究院国防科技大学

本文通过与精确数值解的比较,在相当大的核温度和粘滞性范围内验证了具有非谐位的Fokker-Planck方程的局域谐振近似的可靠性.

关键词：局域谐振近似解核裂变