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Acta Biochimica et Biophysica Sinica 2005年第9期37卷 588-592页

作者： Dong-Ning pAN~(1,2) Jin-Jun LI~(2,3) Lin WEI~2 Ming YAO~(2,3) Da-Fang WAN~(2,3*) Jian-ren GU~(1,2,3*) 1 Shanghai medical college,Fudan university,Shanghai 200032,china 2 National Laboratory for Oncogenes and related Genes,Shanghai Cancer Institute,Shanghai 200032,china 3 medical School of Shanghai Jiaotong university,Shanghai 200032,china Shanghai medical college Fudan UniversityShanghai 200032 China National Laboratory for Oncogenes and related Genes Shanghai Cancer InstituteShanghai 200032 China medical School of Shanghai Jiaotong universityShanghai 200032 China

The expression product of ct120a,a novel gene isolated from human chromosome 17p13.3in our laboratory,was predicted to have seven transmembrane domains and could cause malignanttransformation of mouse NIH3T3 *** existed an mrNA splicing variant of ct120a,namely ct120b,which had a 96-nucleotide deletion and produced an in-frame loss of 32 amino acids from codon 136 tocodon 167 of *** CT120B protein was predicted to have six transmembrane *** thisstudy,we observed that the green fluorescent protein-tagged CT120B was localized on plasma membraneand in cytoplasm in SpC-A-1 *** expression of CT120B/A in normal lung tissue and in lung cancercells was also *** showed that the stable CT120B overexpression in SpC-A-1 cells resultedin a reduction of cell growth rate,and inhibited tumorigenecity and anchorage-independent growth in *** functions of CT120A and CT120B for cell growth appeared *** suggested that thedelayed G1/S phase transition might contribute to the inhibitory activities of CT120B on cell growth and thatthe deleted 32 amino acids missing in CT120B might be essential for the oncogenetic activities of CT120A.

关键词： CT120B alternative splicing cell growth lung cancer

CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation

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Acta pharmacologica Sinica 2007年第1期28卷 66-72页

作者： Zhen-zhong ZHENG Zheng-xiang LIU~2 Department of Cardiology,Tongji Hospital,Tongji medical college,Huazhong university of Science and Technology,Wuhan 430030,china Department of Cardiology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effects of CD 151 on the activity of endothelial NO syn-thase(eNOS),and ECV304 migration,proliferation and tube ***:pAAV-CD 151 and pAAV-anti-CD 151 were constructed and used to transientlytransfect ECV304 mediated with Lipofectamine *** transfection,the ex-pression of CD 151 was measured by Western *** migration assay wasperformed using Boyden transwell;proliferation assay was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5,diphenyltetrazolium bromide(MTT)method,andtube formation test was examined on matrigel,eNOS activity was assayed by L-[~3H]citrulline production from L-[~3H]*** involvement of eNOS was ex-plored using an eNOS inhibitor(L-NAME)and the effects in the process ***:CD 151 promotes cell migration,proliferation and tube *** addition,CD151 increases eNOS ***,cell migration,prolifera-tion and tube formation induced by CD 151 are inhibited when L-NAME is used,which indicates that there is an involvement of eNOS in CD151-induced cellmigration,cell proliferation and tube ***:CD 151 promotesECV304 migration,proliferation and tube *** mechanism is that CD151increases eNOS *** result also suggests that eNOS is involved in theangiogenic effects of CD151.

关键词： CDI51 endothelial NO synthase(eNOS) cell migration cell proliferation tube formation

反义封闭NGAL基因表达对SHEEC食管癌细胞微丝骨架的影响

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生物化学与生物物理进展 2004年第5期31卷 409-415页

作者：林珏龙许丽艳李恩民蔡唯佳牛永东方昆阳熊华淇沈忠英曾毅汕头大学医学院中心实验室汕头515031 汕头大学医学院肿瘤病理研究室汕头515031 汕头大学医学院生物化学与分子生物学教研室汕头515031 中国预防医学科学院病毒学研究所北京100052

为了研究反义封闭NGAL基因表达对SHEEC食管癌细胞微丝骨架以及肿瘤细胞生物学行为的影响 ,以不同长度NGAL基因片段反义表达载体和硫代修饰反义寡核苷酸单链片段转染SHEEC食管癌细胞 ,通过G4 18筛选 ,建立一系列旨在封闭SHEEC食管癌细胞N... 详细信息

为了研究反义封闭NGAL基因表达对SHEEC食管癌细胞微丝骨架以及肿瘤细胞生物学行为的影响 ,以不同长度NGAL基因片段反义表达载体和硫代修饰反义寡核苷酸单链片段转染SHEEC食管癌细胞 ,通过G4 18筛选 ,建立一系列旨在封闭SHEEC食管癌细胞NGAL基因表达的亚细胞克隆 .在细胞内F 肌动蛋白 (F actin)及DNA荧光双标记基础上 ,通过流式细胞术、激光共聚焦显微镜扫描术等技术手段检测封闭反义NGAL基因表达后 ,SHEEC食管癌细胞中F actin和DNA含量、F actin形态结构以及肿瘤细胞生物学行为的变化特征 .结果显示 ,反义封闭NGAL基因表达后 ,SHEEC食管癌细胞F actin的含量明显降低 ,与永生化食管上皮细胞SHEE相近 ,但细胞分裂增殖指数未见明显变化 .表明反义封闭NGAL基因表达对SHEEC食管癌细胞的微丝骨架有明显影响 ,而对SHEEC食管癌细胞的分裂增殖影响不明显 .激光共聚焦显微镜扫描观测显示 ,反义封闭NGAL基因表达可使SHEEC食管癌细胞F actin分布均匀 ,F actin小体减少 ,细胞间连接重新建立 ,结构较紧密 ,主要形态结构特征与SHEE细胞趋于一致 .提示反义封闭NGAL基因表达可对SHEEC食管癌细胞的微丝骨架F actin产生明显影响 ,推测癌细胞的微丝骨架F

关键词：微丝骨架 F-肌动蛋白 NGAL基因 SHEEC细胞细胞周期

In Vitro Study on Vertical Transmission of the HIV-1 gag Gene via Human Sperm

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In Vitro Study on Vertical Transmission of the HIV-1 gag Gen...

广东省环境诱变剂学会、广东省预防医学会卫生毒理专业委员会2010年学术会议

作者： Dian Wang~(1△),Xiang-Jin Kang~(1△),Lian-Bing Li~2,Qing-Dong Xie~1,Yu-Sen Gao~3,Tian-Hua Huang~(1*) 1 research Center for reproductive Medicine,shantou university medical college,shantou 515041,china. 2 Chongqing key laboratory of birth defects and reproductive health,Chongqing,400020,china. 3 The First Affiliated Hospital,shantou university medical college,shantou 515041,china

HIV/AIDS is a major public health problem *** explore the feasibility of HIV vertical transmission via human sperm,plasmid construction and transfection,interspecific in vitro fertilization of zona-free hamster ova by human sperm,fluorescence in situ hybridization(FISH),rT-pCr and immunofluorescence assay(IF) were *** positive FISH signals for HIV-1 gag DNA were observed in the nucleus and chromosome of transfected human sperm,male pronucleus of zygote and in nuclei of blastomeres of two-cell embryo,indicating that HIV-1 gag gene could be transmitted via sperm membrane and integrate into sperm *** the other hand,human sperm carrying target gene could achieve normal fertilization,and replication of sperm-mediated target gene was synchronized with that of the host *** rT-pCr,the positive bands for target gene were observed in the transfected human sperm and two-cell *** results further confirm that target gene can be transcribed into mrNA in human sperm and embryonic *** IF,the positive signals for HIV-1 p24 gag protein was observed in the two-cell embryos containing sperm-mediated target gene and not in the transfected human sperm,which indicated that sperm-mediated target gene could be translated to make HIV-1 p24 gag protein in embryonic cells but not in sperm *** results provided evidences for a possible vertical transmission of HIV-1 gag gene to embryo via a fertilizing sperm in vitro.

关键词： HIV-1 gag gene human sperm integration expression vertical transmission

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非线性代数方程组的扩WESOLVE算法

四川大学学报（自然科学版） 2001年第6期38卷 824-826页

作者：李永彬四川大学数学学院

将WSOLVE算法和扩WE相对单纯分解算法结合起来 ,得到扩WESOLVE分解算法。

关键词：扩WE相对单纯分解算法非混合分解算法 WrSOLVE分解算法非线性代数方程组

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巴氯芬治疗顽固性呃逆

中国新药与临床杂志 2001年第6期20卷 432-433页

作者：陈协辉吴贤仁汕头大学医学院第一附属医院内二科广东汕头515041

目的 :观察巴氯芬对顽固性呃逆的疗效。方法 :80例顽固性呃逆病人分为 2组 ,其中治疗组42例 (男性 2 8例 ,女性 14例 ,年龄 48a±s 8a)采用巴氯芬治疗。最小剂量是 10mg ,bid。最大剂量为 15mg ,tid。小剂量开始 ,逐步递增。疗程 4d。3 ... 详细信息

目的 :观察巴氯芬对顽固性呃逆的疗效。方法 :80例顽固性呃逆病人分为 2组 ,其中治疗组42例 (男性 2 8例 ,女性 14例 ,年龄 48a±s 8a)采用巴氯芬治疗。最小剂量是 10mg ,bid。最大剂量为 15mg ,tid。小剂量开始 ,逐步递增。疗程 4d。3 8例对照组 (男性 2 4例 ,女性 14例 ,年龄 44a± 11a)采用氯丙嗪、苯妥英、硝苯地平、东莨菪碱等常规治疗。结果 :治疗组总有效率 98% ,对照组总有效率 5 8% (p 2 1% (p p <0 .0 1) ,不良反应少。结论 :巴氯芬是治疗顽固性呃逆较为有效的药物。

关键词：巴氯芬氯丙嗪呃逆药物疗法治疗

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论实践的工具意义和伦理意义

山东大学学报（哲学社会科学版） 2002年第5期 54-56页

作者：贾澜汕头大学医学院广东汕头515031

实践作为一个重要的哲学范畴具有丰富的内涵 ,是工具意义与伦理意义的统一。人类社会生活在本质上是实践的 ,而人性以及人类生活的复杂性和丰富性必然使得实践意义具有深刻性和丰富性 ;中外哲学史对实践活动的认识成果给我们进一步反思... 详细信息

实践作为一个重要的哲学范畴具有丰富的内涵 ,是工具意义与伦理意义的统一。人类社会生活在本质上是实践的 ,而人性以及人类生活的复杂性和丰富性必然使得实践意义具有深刻性和丰富性 ;中外哲学史对实践活动的认识成果给我们进一步反思人类这一重要的活动提供了无穷启示 ;时代的飞速发展 ,社会生活所发生的越来越深刻的变化。

关键词：实践工具伦理丰富性

Effect of Ozone produced from Antibody-catalyzed Water Oxidation on pathogenesis of Atherosclerosis

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Acta Biochimica et Biophysica Sinica 2006年第6期38卷 417-422页

作者： Ke-Jun pENG~1,Yu-Shan HUANG~1,Li-Na AN~1,Xiao-Qun HAN~1,Jing-Ge ZHANG~1, Qiu-Lin WANG~2,Jing SUN~1,and Shu-ren WANG~(1*) ~1 Department of pathophysiology,West china preclinical medical and Forensic medical college,Sichuan university,Chengdu 610041,china ~2 The First Department of Internal Medicine,The First Hospital Affiliated to Chengdu medical college,Chengdu 610500,china Department of pathophysiology West China Preclinical Medical and Forensic Medical College Sichuan UniversityChengdu 610041 China The First Department of Internal Medicine The First Hospital Affiliated to Chengdu Medical CollegeChengdu 610500 China

recent studies have suggested that antibodies can catalyze the generation of unknown oxidantsincluding hydrogen peroxide （H2O2） and ozone （O3） from singlet oxygen （1O2） and *** study is aimedto detect the effect of antibody-catalyzed water oxidation on *** results showed that bothH2O2and O_ were produced in human leukemia THp-1 monocytes incubated with human immunoglobulin Gand phorbol myristate *** the THp-1 monocytes incubated with human immunoglobulin G,phorbolmyristate acetate and low density lipoprotein the intracellular total cholesterol,free cholesterol,cholesterylester and lipid peroxides clearly increased,and a larger number of foam cells were observed by oil red *** accumulation of all intracellular lipids was significantly inhibited by vinylbenzoic acid,and onlyslightly affected by *** findings suggested that the production of O3,rather than H2O2,might beinvolved in the pathogenesis of atherosclerosis through the antibody-catalyzed water oxidation pathway.

关键词： antibody ozone THp-1 monocyte foam cell atherosclerosis

Endogenous expression of Neuregulin-1 as a potential modulator of prolactin secretion in GH3 cells

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Endogenous expression of Neuregulin-1 as a potential modulat...