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Partial Rescue of pos5 Mutants by YEF1 and UTR1 Genes in Saccharomyces cerevisiae

Acta Biochimica et Biophysica Sinica 2006年第5期38卷 293-298页

作者： Yong-Fu LI~1 Feng SHI~(2.)1 School of Food Science and Technology2.The key laboratory of Industrial Biotechnology,{2. of education,Southern Yangtze university.Wuxi 2.4036,china School of Food Science and Technology Ministry of Education Southern Yangtze UniversityWuxi 214036 China The key laboratory of Industrial Biotechnology Ministry of Education Southern Yangtze UniversityWuxi 214036 China

Three NAD kinase homologs,encoded by UTR1,POS5 and YEF1 genes,are found in the yeastSaccharomyces cerevisiae and proven to be important sources of NADPH for the ***5p,existing in themitochondrial matrix,is critical for higher temperature endurance and mitochondrial functions,such asglycerol usability and arginine *** constructing the high-copy expression plasmids ofYEF1 and UTR1,which contained the green fluorescent protein reporter tag at their 3’ terminus,and introducingthem into POS5 gene deletion mutants(***5,utr1pos5,yef1pos5 and utr1yef1pos5),the high-copy YEF1and UTR1 plasmids carrying transformants for pos5 mutants were *** temperature sensitivityand growth phenotype on media with glycerol as the sole carbon source,or on media without arginine,*** showed the partial rescue of mitochondrial dysfunctions and temperature sensitivity ofpos5 mutants by the high-copy YEF1 gene,and of glycerol growth defect and temperature sensitivity by thehigh-copy UTR1 gene,which confirmed the potential supplying ability of Yeflp and Utrlp for mitochondrialNADP(H)and implied the weak transport of NADP from cytosol to ***,even throughthe green fluorescent protein reporter label,the subcellular localization of Yef1p and Utr1p in yeast cells couldnot be observed,which indicated the low expression level of these two NAD kinase homologs.

关键词： NAD kinase Saccharomyces cerevisiae mitochondrial function temperature sensitivity subcellular localization

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SHRIMP Zircon U-Pb Ages and Geochemical Characteristics of the Neoproterozoic Granitoids in the Huangling Anticline and Its Tectonic Setting

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Journal of Earth Science 2012年第5期23卷 659-675页

作者：魏运许彭松柏蒋幸福彭中勤彭练红李志宏周鹏曾雄伟 wuhan Institute of Geology and Minerals resources Wuhan Center of Geological Survey Faculty of Earth Sciences China University of Geosciences Three Gorges Research Center for Geo-hazard Ministry of EducationChina University of Geosciences State key laboratory of Geological Processes and Mineral {2. China University of Geosciences

SHRIMP zircon U-Pb dating of the Neoproterozoic Maoping （茅坪） series （Sandouping （三斗坪） rock suite） granites exposed in the southern part of the Huangling （黄陵） anticline shows that the formation time of Sandouping biotite-hornblende tonalite intrusion, Jinpansi （金盘寺） hornblende-biotite tonalite intrusion, and Longtanping （龙潭坪） monzogranite are 863±9, 842.10, and 844±10 Ma, respectively. Their geochemical features include A/CNK=0.98-1.06, from metaluminous to weakly peraluminous, δ=1.37-1.53, Sm/Nd=0.17-0.2., and RbN/YbN=1.1-3.62. These indicate that the granite rocks are supersaturated SiO2.calc-alkaline granitoids. The characteristic of Sr-Nd isotopic composition is that the values of εNd（t） and εsr（t） are -12.4 to -11.0 and 2..2.32.2. respectively. It also suggests that the material source of the granite rocks mainly originated from the crust, and they formed in a volcanic arc tectonic environment. These facts suggest that the occurrence of Neoproterozoic granitoids in the southern part of the Huangling anticline should be related to an arc environment along an active continental margin caused by southward subduction of oceanic crust beneath the northern Yangtze craton, and the formation age is not later than 863 Ma.

关键词： Huangling anticline Yangtze plate Neoproterozoic granitoid SHRIMP zircon U-Pbdating geochemistry tectonic setting.

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Energy analysis for damage and catastrophic failure of rocks

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Science china(Technological Sciences) 2011年第S1期54卷 199-209页

作者： XIE HePing 1,2.LI LiYun 2.1,JU Yang 2.PENG RuiDong 2.& YANG YongMing 2.1 Sichuan university,Chengdu 610065,china 2.State {2. laboratory of Coal resources and Safe Mining,Beijing {2. laboratory of Fracture and Damage Mechanics of Rock and Concrete,china university of Mining and Technology,Beijing 100083,china Sichuan university Chengdu China State key laboratory of Coal {2. and Safe Mining Beijing Key Laboratory of Fracture and Damage Mechanics of Rock and Concrete China University of Mining and Technology Beijing China

The development history and current state of studies on the characteristics and mechanisms of deformation and failure of rock materials were briefly reviewed from the viewpoint of *** main scope and the achievable objectives of the energy-based research system were *** was validated by experiments that the damage process of rocks can be well described by the rock damage evolution equation established based on energy *** was found from the uniaxial compression and biaxial compression tests that only a small proportion of the total input energy in hard rocks is dissipated before peak load and a large proportion in soft rocks is dissipated before peak *** both hard and soft rocks,the energy dissipated after peak load accounts for a greater *** energy would be required for rock failure under equal biaxial compression than under unequal biaxial *** total absorbed energy is different for rock failure under high-rate loading and low-rate *** fragmented failure pattern usually corresponds to higher energy *** mesoscopic analysis on the damage and failure of bedded salt rocks showed that the energy dissipation is prominent and the total absorbed energy for rock failure is low when cracks propagate in the weak mud interlayer while it is contrary when cracks propagate in the salt *** energy accumulation,transfer,dissipation and release during the failure process of tunnel with impending failure under disturbance were analyzed theoretically based on the elastoplastic mechanics ***,the spatial distribution of energy dissipation and energy release of fractured rocks under unloading was simulated *** was demonstrated that energy is likely to be released from the weakest surface under compression,which triggers the global failure of rocks.

关键词： rock deformation failure energy accumulation energy dissipation energy release

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松辽盆地北部姚家组底界面特征及其动力学背景

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石油学报 2010年第2期31卷 187-195页

作者：宋鹰任建业阳怀忠佟殿君雷超中国地质大学构造与油气资源教育部重点实验室湖北武汉430074 中国地质大学资源学院湖北武汉430074 教育部含油气盆地构造研究中心中国地质大学分中心湖北武汉430074

以松辽盆地北部地震资料精细构造解释和钻井资料分析为基础,对其姚家组底界面特征展开了详细的研究。结果表明:在该界面之下的地层内发育了4组近NNE走向长距离延伸的微幅褶皱带,并且这4组褶皱带的隆起幅度由东向西逐渐减小;沿该界面发... 详细信息

以松辽盆地北部地震资料精细构造解释和钻井资料分析为基础,对其姚家组底界面特征展开了详细的研究。结果表明:在该界面之下的地层内发育了4组近NNE走向长距离延伸的微幅褶皱带,并且这4组褶皱带的隆起幅度由东向西逐渐减小;沿该界面发育了东薄西厚的红色古土壤层;古生物资料也显示界面上下有明显的物种组合突变现象。地球物理、沉积、古生物等证据揭示,姚家组底界面是发育在盆地裂后热沉降期内的一个具有挤压背景的区域性角度不整合面。磁性地层、古生物和同位素年龄均显示该界面的发育时代为晚白垩纪(距今约88.5Ma),与中生代期间欧亚板块与太平洋板块间一次重大的板块构造运动学重组事件一致。由此推断,松辽盆地北部姚家组底界面是晚白垩纪板块构造重组事件在松辽盆地内的响应界面,至此开始,松辽盆地进入了构造反转演化阶段。

关键词：松辽盆地姚家组底界面板内应力构造反转晚白垩纪板块构造

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Mechanism for calcite dissolution and its contribution to development of reservoir porosity and permeability in the Kela 2.gas field,Tarim Basin,china

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Science china Earth Sciences 2008年第4期51卷 567-578页

作者： YU BingSong1,3, DONG HailLiang2.3 & RUAN Zhuang1,3 1 State key laboratory of Geological Processes and Mineral {2., china university of geosciences, Beijing 100083, china 2.Department of Geology, Miami university,Oxford, OH 45056, USA 3 Geomicrobiology laboratory, china university of geosciences, Beijing, 100083, china State key laboratory of Geological Processes and Mineral {2. China University of Geosciences Beijing China Geomicrobiology laboratory China University of Geosciences Beijing China Department of Geology Miami University Oxford USA

This study is undertaken to understand how calcite precipitation and dissolution contributes to depth-related changes in porosity and permeability of gas-bearing sandstone reservoirs in the Kela 2.gas field of the Tarim Basin, Northwestern china. Sandstone samples and pore water samples are col-lected from well KL2.1 in the Tarim Basin. Vertical profiles of porosity, permeability, pore water chem-istry, and the relative volume abundance of calcite/dolomite are constructed from 3600 to 4000 m below the ground surface within major oil and gas reservoir rocks. Porosity and permeability values are in-versely correlated with the calcite abundance, indicating that calcite dissolution and precipitation may be controlling porosity and permeability of the reservoir rocks. Pore water chemistry exhibits a sys-tematic variation from the Na2.O4 type at the shallow depth (3600-3630 m), to the NaHCO3 type at the intermediate depth (3630―3695 m),and to the CaCl2.type at the greater depth (372.―3938 m). The geochemical factors that control the calcite solubility include pH, temperature, pressure, Ca2. concen-tration, the total inorganic carbon concentration (ΣCO2., and the type of pore water. Thermodynamic phase equilibrium and mass conservation laws are applied to calculate the calcite saturation state as a function of a few key parameters. The model calculation illustrates that the calcite solubility is strongly dependent on the chemical composition of pore water, mainly the concentration difference between the total dissolved inorganic carbon and dissolved calcium concentration (i.e., [ΣCO2. -[Ca2.]). In the Na2.O4 water at the shallow depth, this index is close to 0, pore water is near the calcite solubility. Calcite does not dissolve or precipitate in significant quantities. In the NaHCO3 water at the intermedi-ate depth, this index is greater than 0, and pore water is supersaturated with respect to calcite. Massive calcite precipitation was observed at this depth interval and

关键词： calcite dissolution porosity thermodynamic Tarim Basin

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Mining subsidence control by solid backfilling under buildings

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中国有色金属学会会刊：英文版 2011年第S3期21卷 670-674页

作者： ZHA Jian-feng1, 2. 3, GUO Guang-li1, 2. FENG Wen-kai3, WANG Qiang1, 2.1. key laboratory for Land Environment and Disaster Monitoring of the State Bureau of Surveying and Mapping, {2. university of Mining and Technology, Xuzhou 2.1116, {2. 2. Jiangsu key laboratory of resources and Environmental Information Engineering, {2. university of Mining and Technology, Xuzhou 2.1116, {2. 3. State key laboratory of Geohazard Prevention and Geoenvironment Protection, Chengdu university of Technology, Chengdu 610059, {2. key laboratory for Land Environment and Disaster Monitoring of the State Bureau of Surveying and Mapping China University of Mining and Technology Xuzhou 221116 China Jiangsu key laboratory of {2. and Environmental Information Engineering China University of Mining and Technology Xuzhou 221116 China State key laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology Chengdu 610059 China

Solid backfilling mining can reduce the buildings' damage caused by mining greatly. The reduction of subsidence value, the slow advancing speed and the subsidence caused by backfilling body compaction are the main reasons that solid backfilling mining velocity decreases significantly. Based on the research of mechanism, some principles on subsidence control of solid backfilling mining under buildings were proposed. The equivalent mining height was designed according to the fortification criteria of buildings and their attachment structures, which enables the ground movement and deformation caused by mining to be less than the corresponding fortification criteria.

关键词： solid backfilling mining subsidence control mining under buildings

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Wave tectono-sedimentary processes in Tarim basin

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Science china Earth Sciences 2005年第11期48卷 1949-1959页

作者： JIN Zhijun1,2. ZHANG Yiwei1 & CHEN Shuping1 1. key laboratory for Hydrocarbon Accumulation, {2. of education, china university of Petroleum, Beijing 102.49, china 2. Exploration and Production Research Institute, china Petroleum & Chemical Corporation, Beijing 100083, china key laboratory for Hydrocarbon Accumulation {2. of education China University of Petroleum Beijing China Exploration and Production Research Institute China Petroleum & Chemical Corporation Beijing China

Based on the unconformities, the formation times of structures, and geological wave filtering applied to basin fills of typical wells around the Manjiaer area in the Tarim basin, the wave tectono-sedimentary processes in the Tarim Basin are discussed. The results of geological wave filtering of basin fills of typical wells show that, in time domain, the evolution of the Tarim basin was controlled by various wave processes with the periods of 740±Ma, 2.0±Ma, 100±Ma and 30±Ma. The analyses of basin fills, basin subsidence and unconformities show that the trends of the sedimentary facies belts, depocenters, and centers of subsidence migrated and interchanged periodically with periods of 2.0±Ma. These show the propagation of wave movements in space domain. The wave evolution of the Tarim basin is of significance to understanding the formation of the oil and gas reservoirs in the basin.

关键词： Tarim basin, tectonics, sedimentation, crustal undation, wave evolution

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Quick image-processing method of UAV without control points data in earthquake disaster area

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中国有色金属学会会刊：英文版 2011年第S3期21卷 523-528页

作者： LI Chang-chun1, ZHANG Guang-sheng1, LEI Tian-jie2. 3, GONG A-du2. 3 1. School of Surveying & Land Information Engineering, Henan Polytechnic university, Jiaozuo 454000, china 2. {2. laboratory of Environmental Change and Natural Disaster, ministry of education, Beijing Normal university, Beijing 100875, china 3. ministry of Civil Affairs/ministry of education of china Academy of Disaster Reduction and Emergency Management, Beijing Normal university, Beijing 100875, china School of Surveying & Land Information Engineering Henan Polytechnic University Jiaozuo 454000 China key laboratory of Environmental Change and Natural Disaster Ministry of Education Beijing Normal University Beijing 100875 China ministry of Civil Affairs/ministry of {2. of china Academy of Disaster Reduction and Emergency Management Beijing Normal University Beijing 100875 China

The methods of unmanned aerial vehicles images splicing rapidly and rectifying without control points data, only using auxiliary data recorded by unmanned aerial vehicle (UAV) system, were researched. Two algorithms were proposed, including UAV images auto- splicing rapidly after image blocking and UAV images rectifying based on pseudo center points of auxiliary data. When SIFT algorithm for UAV images splicing is applied, the accuracy is very high, but the efficiency is very low. The splicing efficiency is improved greatly based on SIFT algorithm after image blocking. The results show that rectifying accuracy is very high and has no visual dislocation when pseudo center points of auxiliary data is applied.

关键词： unmanned aerial vehicles SIFT algorithm blocking quickly splicing rectification

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A study on odd-parity high-lying states of the Sm atom with three-color resonant excitation

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Science china(Physics,Mechanics & Astronomy) 2011年第6期54卷 1124-1130页

作者： LI Ming 1,2.DAI ChangJian 1,2.& XIE Jun 1,2.1 key laboratory of Display Materials and Photoelectric Devices,{2. of education,Tianjin 300191,china 2.School of Science,Tianjin university of Technology,Tianjin 300384,china key laboratory of Display Materials and Photoelectric Devices Ministry of Education Tianjin China School of Science Tianjin University of Technology Tianjin China

The odd-parity high-lying states of the Sm atom are investigated systematically by a three-color resonant excitation scheme with two different excitation *** two intermediate states,4f 6 6s7s 7 F 0 and 4f 6 6s7s 7 F 1 are employed for paths I and II,***-seven bound states are detected with paths I and II in the energy range between 44188 and 45515 cm 1,while 64 autoionizing states are found in the energy range from 4552. to 45761 cm *** only the level energies of all observed states,but also the line widths of autoionizing states are *** possible impact of configuration interaction on the line shape of autoionizing states is also *** addition,the total angular momentum is assigned uniquely to most of the states,whereas the information about the relative line intensity of all states is also presented.

关键词： high-lying states odd-parity the Sm atom resonant excitation

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Test of magnetic susceptibility and grain-size age models of loess

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Progress in Natural Science:Materials International 2007年第11期17卷 1312-1317页

作者： Chen Yimeng1,2.3, Feng Zhaodong4 and Gong Huili1,2.1. MOE and key laboratory of {2. Environment and GIS of Beijing, Beijing 100037, china 2. key laboratory of 3D information Acquisition and Application, The Capital Normal university, Beijing 100037, china 3. Department of Economics & Management of Huizhou university, Huizhou 516007, china 4. key laboratory of Western china’s Environmental Systems, ministry of education, Lanzhou university, Lanzhou 730000, china) key laboratory of {2. Environment and GIS of Beijing Beijing 100037 China key laboratory of 3D information Acquisition and Application Capital Normal University Beijing 100037 China Department of Economics and Management Huizhou University Huizhou 516007 China key laboratory of Western {2.'s Environmental Systems Lanzhou University Lanzhou 730000 China

Ages of the stratigraphic boundary MIS1/2.and MIS3/4 of the Yuanbu loess section in Linxia are used as the basis of the nodal control age. The age of MIS1/2.and MIS3/4 are obtained from the latest international research result—the climatic events recorded in the stalagmite in the Hulu Cave in Nanjing, that MIS1/2.is 11.5 kaB.P. and MIS3/4 is 59.8 kaB.P.. The ages of the two climatic events contain three nodal age control models (Model 1: 0 kaB.P.—59.8 kaB.P.; Model 2. 0 kaB.P.—11.5 kaB.P. and 11.5 kaB.P.—59.8 kaB.P.; Model 3: 11.5 kaB.P.—59.8 kaB.P.), which are used as the nodal control age separately. The deposition times of various stratigraphic horizons are calculated by using the magnetic susceptibility age model and grain-size age model, and then compared with each other. In addition, the AMS14C age, OSL age and the ages of YD and H events are compared with the ages of the corresponding horizons calculated by the three models of nodal control ages. From the analyses of lithologic characters and climatic stages it has been found that both the magnetic susceptibility age model and the grain-size age model have some defects. Because the accurate control ages are selected as the nodal points of the glacial period or interglacial period, the stratigraphic deposition times determined by the high resolution of magnetic susceptibility age model and grain-size age model approximate to the actual ages. As for the relative accuracy of the two age models, the magnetic susceptibility age model is more accurate than the grain-size age model.

关键词： loess nodal control age magnetic susceptibility age model grain-size age model test

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