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Recombinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice

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Acta Pharmacologica Sinica 2004年第6期25卷 81-86页

作者： Yong WU, Yuan-zhong CHEN2, Hui-fang HUANG, Ping CHEN Fujian Institute of Hematology, Union Hospital, Fujian medical University, Fuzhou 350001,china Fujian Institute of Hematology Union Hospital Fujian Medical University Fuzhou 350001

AIM: To study the preventive effect of recombinant human fibronectin (rhFN) polypeptide on hepatic failure in- duced by endotoxin in mice. METHODS: A cDNA fragment coding for Ile1363-Tyr1725 of human FN was inserted into the PinPoint Xa-3 plasmid, and the constructed plasmid was transformed into E coli BL21 (DE3) cells, and then the expression of rhFN polypeptide in DE3 cells was identified through SDS-PAGE. The target protein from the supernatant of bacteria lysate was purified through biotin-affinity chromatography. The bioactivity of the purified rhFN polypeptide was determined with cell adhesiveactivity. The survival rate was observed in endotoxemia mice injected with rhFN polypeptide. The tissue damage in hepatocyte was detected using histology, ultrastructure, and DNA fragmentation assay. RESULTS: The expression of rhFN polypeptide reached approximately 20 % of the total cellular protein. The adhesion ability of rhFN polypeptide was concentration-dependent. The value of EC50 was 0.8 nmol/L. The survival rate of endotoxemia mice sensitized by D-galactosamine (D-GalN) was 60 % in rhFN polypeptide treated group, while that of endotoxemia mice sensitized by D-GalN was 20 % in the control group (P<0.01). Histopathology showed that less necrosis occurred on the hepatocyte of endotoxemia mice in- jected with rhFN polypeptide compared with saline control. Ultrastructure and DNA fragmentation assay showed that no apoptotic hepatocyte was observed in the liver of rhFN-treated endotoxemia mice. CONCLUSION: Re- combinant fibronectin polypeptide antagonizes hepatic failure induced by endotoxin in mice.

关键词： fibronectins recombinant fusion proteins lipopolysaccharides acetylgalactosamine

含时空关联噪声的非局域及各向异性Kardar-Parisi -Zhang方程的直接标度分析

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物理学报 2002年第5期51卷 994-998页

作者：唐刚马本堃中国矿业大学物理系徐州221008 北京师范大学物理系和理论物理研究所北京100875

将直接标度分析方法推广应用到含时间空间关联噪声的非局域及各向异性Kardar Parisi Zhang方程的动力学标度分析中 ,分别得到了方程在强耦合区和弱耦合区的标度指数值 .

关键词：时空关联噪声非局域各向异性直接标度分析 KPZ方程表面界面动力学粗化生长

腹腔镜结直肠癌根治术操作指南(2023版)

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中华消化外科杂志 2024年第1期23卷 10-22页

作者：中华医学会外科学分会腹腔镜与内镜外科学组中华医学会外科学分会结直肠外科学组中国医师协会外科医师分会结直肠外科专家工作组中国抗癌协会腔镜与机器人外科分会中国医学装备协会腔镜与微创技术分会郑民华张忠涛叶颖江不详上海交通大学医学院附属瑞金医院普通外科胃肠外科、上海市微创外科临床医学中心上海200025 首都医科大学附属北京友谊医院普通外科、国家消化系统疾病临床医学研究中心北京100050 北京大学人民医院胃肠外科北京100044

腹腔镜技术目前已是结直肠癌根治手术治疗的重要手段。近年,随着我国腹腔镜结直肠癌根治手术的进一步发展与普及,手术技术及理念已发生较大变化和更新,全世界范围内不断有高质量临床研究开展和循证医学证据发布。腹腔镜结直肠癌根治手... 详细信息

腹腔镜技术目前已是结直肠癌根治手术治疗的重要手段。近年,随着我国腹腔镜结直肠癌根治手术的进一步发展与普及,手术技术及理念已发生较大变化和更新,全世界范围内不断有高质量临床研究开展和循证医学证据发布。腹腔镜结直肠癌根治手术在手术指征、腔镜显示系统、手术入路、全腔镜手术及腹腔镜下关键解剖标志和操作要点等方面均有较多进展。基于《腹腔镜结直肠癌手术操作指南(2018版)》,结合近年研究热点及高等级循证医学成果,以临床证据为基础,临床问题为导向,中华医学会外科学分会腹腔镜与内镜外科学组、中华医学会外科学分会结直肠外科学组、中国医师协会外科医师分会结直肠外科专家工作组、中国抗癌协会腔镜与机器人外科分会、中国医学装备协会腔镜与微创技术分会组织国内相关领域专家再次修订更新形成《腹腔镜结直肠癌根治术操作指南(2023版)》。

关键词：结直肠肿瘤根治手术腹腔镜检查指南

Cytocompatibility of Self-assembled Hydrogel from IKVAV-containing Peptide Amphiphile with Neural Stem Cells

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Journal of Wuhan University of Technology(Materials Science) 2009年第5期24卷 753-756页

作者：宋玉林 Department of Orthopaedics the Second Affiliated Hospital of Nanchang University

Neural Stem Cells （NSCs） were incubated with self-assembled hydrogel from IKVAV-containing peptide amphiphile （IKVAV-PA） for one week. The cytocompatibility of hydrogel was evaluated. NSCs were seeded in three-dimensional （3D） hydrogels （Experimental group, EG） or surface of coverslips （Control group, CG）, double-labeled with Calcein-AM and PI. A growth curve of cells was obtained according to CCK-8. TEM study of hydrogel revealed a network of nanofibers. NSCs began to proliferate after 24 h of incubation, and formed bigger neurospheres at 48 h in EG than in CG. Cell proliferation activity was higher in EG than in CG （P〈0.05）. The self-assembled Hydrogel had good cytocompatibility and promoted the proliferation of NSCs.

关键词： IKVAV hydrogel cytocompatibility proliferation

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《中国卒中中心报告2022》概要

中国脑血管病杂志 2024年第8期21卷 565-576页

作者：《中国卒中中心报告2022》编写组王陇德《中国卒中中心报告2022》编写组

随着社会老龄化、城市化进程加速和民众不健康生活方式的流行,我国卒中疾病负担呈现快速增长态势。在国家卫生健康委员会的领导下,在全国各级卫生健康主管部门的大力支持以及疾病预防管理机构、医疗卫生机构与广大医务人员的积极参与下... 详细信息

随着社会老龄化、城市化进程加速和民众不健康生活方式的流行,我国卒中疾病负担呈现快速增长态势。在国家卫生健康委员会的领导下,在全国各级卫生健康主管部门的大力支持以及疾病预防管理机构、医疗卫生机构与广大医务人员的积极参与下,我国卒中防治工作取得了显著成效。自2021年至2022年,我国卒中中心的建设以行政为主导,在各级卫生健康行政部门的组织落实和推进下,开展了各医疗机构的资源整合、流程优化和学科协作行动,建立了高效的救治绿色通道,提供了规范化的诊疗服务,并打造了“以患者为中心”的卒中专病诊疗模式,初步实现了极具中国特色的“防、治、管、康、健”五位一体的全流程卒中防治体系。《中国卒中中心报告2022》对卒中流行病学、卒中中心建设进展、卒中防治关键适宜技术开展与其相关指标的分析等方面进行了系统回顾,梳理了2022年卒中中心工作进展并对未来工作进行展望。

关键词：卒中流行病学卒中中心卒中防治关键适宜技术

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新型冠状病毒感染影像学诊断标准

武汉大学学报(医学版) 2024年第10期45卷 1151-1158+1182页

作者：廖美焱刘军刘晋新张寒菲赵伟林琳胡天丽中华医学会放射学分会传染病学组中国研究型医院学会感染与炎症放射专业委员会中国医院协会传染病分会传染病影像学组北京影像诊疗技术创新联盟武汉大学中南医院中南大学湘雅二医院广州医科大学附属市八医院

新型冠状病毒感染（COVID-19）目前为全球性重大的公共卫生事件，诊断需有病原学或血清学证据，疫苗接种者和儿童COVID-19症状轻。影像评估是COVID-19临床诊断、鉴别诊断、疗效监测和预后随访的主要和常用手段，资源有限时影像学检查适... 详细信息

新型冠状病毒感染（COVID-19）目前为全球性重大的公共卫生事件，诊断需有病原学或血清学证据，疫苗接种者和儿童COVID-19症状轻。影像评估是COVID-19临床诊断、鉴别诊断、疗效监测和预后随访的主要和常用手段，资源有限时影像学检查适用于中重度临床特征COVID-19疑似和高概率患者的医学分诊。推荐高分辨率CT评估COVID-19肺损伤及随访，重症患者使用便携式数字X线检查，孕妇和儿童可使用MRI检查评估疗效，COVID-19人工智能诊断可用于发热患者早期筛查。典型COVID-19影像学演变分为早期、进展期、重症期、恢复期四个阶段，早期为胸膜下局限分布的斑片状、亚段或节段性磨玻璃样影（GGO）；进展期病灶范围增大、增多，累及多个肺叶；重症期双肺弥漫性实变或GGO，呈“白肺”表现；恢复期病变吸收。大部分非重患者病变可完全吸收。部分患者出现COVID-19后综合征，3个月后肺部见低密度区，GGO伴马赛克征、条索影、网状或蜂窝样改变、支气管扩张、肺气囊、肺纤维化等表现。临床中型以GGO病变为主，重型/危重型GGO范围增大、实变增多，双肺多叶受累多见。COVID-19肺炎需与其他病毒引起的肺炎、严重急性呼吸综合征、隐源性机化性肺炎、过敏性肺炎等鉴别。

关键词：新型冠状病毒 COVID-19 肺炎呼吸道疾病影像诊断标准

Nonlinear optical properties and superluminal propagation in the ruby

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Chinese Science Bulletin 2010年第6期55卷 473-477页

作者： GAO Feng1,2,3,XU JingJun1,2,3,ZHANG GuoQuan1,2,3 & ZHANG WenDing1,2,3 1 Photonics Center,college of physics Science,Nankai University,Tianjin 300457,china 2 The Key Laboratory of Advanced Technique and Fabrication for Weak-Light Nonlinear Photonics Materials of Ministry of Education,Nankai University,Tianjin 300457,china 3 Tianjin Key Laboratory of Photonics Materials and Technology for Information Science,Nankai University,Tianjin 300457,china Photonics Center College of Physics Science Nankai University Tianjin China The Key Laboratory of Advanced Technique and Fabrication for Weak-Light Nonlinear Photonics Materials of Ministry of Education Nankai University Tianjin China Tianjin Key Laboratory of Photonics Materials and Technology for Information Science Nankai University Tianjin China

In this review article,we summarized the study of the weak light nonlinear properties associated with group velocity change in the *** ruby could be reduced to a two-level system,in which population oscillations could lead to a subluminal *** the modulated TEM00 beam introduced into the ruby,self-phase modulation together with Fraunhofer diffractions and nondegenerate two-wave coupling between different spatial frequency components of the beam occurs at the same *** oscillations,self-phase modulation together with Fraunhofer diffractions and nondegenerate two-wave coupling mechanism competes with each other,which brings a new phenomenon of self-superluminal group velocity propagation in the ruby.

关键词：非线性光学性质超光速传播红宝石自相位调制 TEM00模速度变化光束耦合空间频率

Implication of Expression of Osteopontin and Its Receptor Integrin αvβ3 in the Placenta in the Development of Preeclampsia

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Journal of Huazhong University of Science and Technology(medical Sciences) 2009年第6期29卷 755-760页

作者：夏俊霞乔福元苏放明刘海意 Department of Obstetrics and Gynecology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Obstetrics Shenzhen People’s Hospital the Second Clinical Medical College of Jinan University

To investigate the expression of osteopontin （OPN） and its receptor integrin αvβ3 in the placental tissue from pregnant women complicated with preeclampsia, the expression of OPN and αvβ3 in the placenta of the pregnant women with preeclampsia and healthy pregnant women was detected by immunohistochemistry, Western blotting and RT-PCR. Our results showed that OPN and αvβ3 protein were expressed in the placenta from normal pregnant woman and those with preeclampsia. OPN was located in the placental syncytiotrophoblasts and the cytoplasm of capillary endothelial cells and integrin αvβ3 was mainly expressed on the surface of trophoblast cells. Expression of OPN and integrin αvβ3 in the placental tissue from preeclampsia subjects was significantly lower than that from the control group （P〈0.05）. Compared with the control group, expression of OPN in the placental tissue from preeclampsia group was significantly lower （P〈0.05） but there was no significant difference in the expression of αv and β3 between the preeclampsia group and the controls. It is concluded that OPN and its receptor integrin αvβ3 may be involved in the pathogenesis of preeclampsia.

关键词： osteopontin integrin αvβ3 placenta preeclampsia

Effects of Atractylodes Macrocephala on the Cytomembrane Ca^(2+)-activated K^+ Currents in Cells of Human Pregnant Myometrial Smooth Muscles

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Journal of Huazhong University of Science and Technology(medical Sciences) 2008年第2期28卷 200-203页

作者：章小莉汪琳徐龙邹丽 Department of Gynecology and Obstetrics Union HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430022China Department of Histology and Embryology the Medical College of Wuhan University Wuhan 430060China Department of Gastroenterology the People's Hospital of Wuhan University Wuhan 4300China

The study examined the inhibitory effect of Atractylodes macrocephala （AM） on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the Ca^2＋-activated K^＋ currents of pregnant human myometrial smooth muscle cells with or without the treatment with intedeukin-6. Single cells were acutely isolated from pregnant human myometrial smooth muscles. Whole-cell Ca^2＋-activated K^＋ currents were recorded by using an Axopatchl-D amplifier. The cells were divided into three groups： group A in which AM was added into perfusate, group B, in which interleukin-6 was added into perfusate） and group C in which AM was added into perfusate after addition of interleukin-6. IL-6 10 ng/mL inhibited BKca by 36.9%±13.7% as compared with control （P〈0.01）. AM at 2 mg/mL raised BKca by 36.7%±22.6% or 45.2%±13.7% with or without the treatment of IL-6, respectively （P〈0.01）. It is concluded that AM was able to enhance the BKca of pregnant human myometrial smooth muscle cells treated or untreated with interleukin-6 and its effect on the BKca IL-treated cells was stronger that its effect on BKca of untreated cells. Our results suggested that AM can help to maintain the membrane potentials and the resting status of pregnant human myometrial smooth muscle cells.

关键词： atractylodes macrocephala interleukin-6 patch clamp, Ca^2+-activated K^+ currents myometrial smooth muscle cells premature delivery