Effects of Atractylodes Macrocephala on the Cytomembrane Ca^(2+)-activated K^+ Currents in Cells of Human Pregnant Myometrial Smooth Muscles

作     者：章小莉 汪琳 徐龙 邹丽 Xiaoli ZHANG1,Lin WANG2,Long XU3,Li ZOU1 1Department of Gynecology and Obstetrics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China 2Department of Histology and Embryology,the Medical College of Wuhan University,Wuhan 430060,China 3Department of Gastroenterology,the People’s Hospital of Wuhan University,Wuhan 4300,China

作者机构：Department of Gynecology and ObstetricsUnion HospitalTongji Medical CollegeHuazhong University of Science and Technology Wuhan 430022China Department of Histology and Embryologythe Medical College of Wuhan University Wuhan 430060China Department of Gastroenterologythe People's Hospital of Wuhan University Wuhan 4300China 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2008年第28卷第2期

页      面：200-203页

核心收录：

学科分类：0710[理学-生物学] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

主　　题：atractylodes macrocephala interleukin-6 patch clamp, Ca^2+-activated K^+ currents myometrial smooth muscle cells premature delivery 

摘      要：The study examined the inhibitory effect of Atractylodes macrocephala （AM） on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the Ca^2＋-activated K^＋ currents of pregnant human myometrial smooth muscle cells with or without the treatment with intedeukin-6. Single cells were acutely isolated from pregnant human myometrial smooth muscles. Whole-cell Ca^2＋-activated K^＋ currents were recorded by using an Axopatchl-D amplifier. The cells were divided into three groups： group A in which AM was added into perfusate, group B, in which interleukin-6 was added into perfusate） and group C in which AM was added into perfusate after addition of interleukin-6. IL-6 10 ng/mL inhibited BKca by 36.9%±13.7% as compared with control （P〈0.01）. AM at 2 mg/mL raised BKca by 36.7%±22.6% or 45.2%±13.7% with or without the treatment of IL-6, respectively （P〈0.01）. It is concluded that AM was able to enhance the BKca of pregnant human myometrial smooth muscle cells treated or untreated with interleukin-6 and its effect on the BKca IL-treated cells was stronger that its effect on BKca of untreated cells. Our results suggested that AM can help to maintain the membrane potentials and the resting status of pregnant human myometrial smooth muscle cells.