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从文献计量学、科学计量学到科学技术计量学——关于科学技术计量学的概念及其研究内容

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图书馆论坛 2003年第6期23卷 151-153页

作者：罗式胜中山大学信息管理系广东广州510275

文献计量学分支学科体系的成功建立为科学计量学的发展提供了坚实的基础。不过从目前国内外的科学计量学研究趋势来看 ,其研究较多是集中在科学领域 ,关于技术领域的计量学研究比较单薄。为此 ,文章试论述科学技术计量学的概念及其研究... 详细信息

文献计量学分支学科体系的成功建立为科学计量学的发展提供了坚实的基础。不过从目前国内外的科学计量学研究趋势来看 ,其研究较多是集中在科学领域 ,关于技术领域的计量学研究比较单薄。为此 ,文章试论述科学技术计量学的概念及其研究的内容等若干问题。

关键词：文献计量学科学计量学科学技术计量学科研投入科研评价布拉德福定律高技术产业

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色彩管理的体系机理与模型分析

计算机科学 2004年第12期31卷 1-5页

作者：柴啸龙罗笑南中山大学计算机应用研究所

彩色图像在不同I/O设备中的色彩表达基于不同色域机制,即使基于相同色域的不同设备常常又有较大的特性差异,因此在不同图像设备和系统平台间提供高品质的色彩再现一直困扰着工业界。该文对目前各种色彩管理体系机理及模型进行了系统的分... 详细信息

彩色图像在不同I/O设备中的色彩表达基于不同色域机制,即使基于相同色域的不同设备常常又有较大的特性差异,因此在不同图像设备和系统平台间提供高品质的色彩再现一直困扰着工业界。该文对目前各种色彩管理体系机理及模型进行了系统的分析,评价和总结,对典型方法分析归纳了主要思想和机理。对研究和应用现状进行了系统总结。最后通过对目前存在问题的分析,指出今后研究出路及应用趋势。

关键词： I/O设备色域彩色图像系统平台色彩再现色彩管理方法分析工业界模型分析体系

A Population-based Study of Lens Opactities

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Eye science 1996年第3期16卷 115-117页

作者： Jingjing Xu, Qiang Yu, Siping Zhu, Qing LiuZhongshan Ophthalmic Center, sun yat-sen university of Medical {1.s,guangzhou 51.060,China Zhongshan Opthalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

Purpose: Age-related lens opacity is the main cause of vision disablitity in elderly population all over the world. This study was designed to investigate the prevalence and incidence of lens ***: 1.1. eyes of 932 adults over 45 year-old were sampled randomly from 4 villeges within Doumen, a county located in the south of Guangdong provience, China. The procedure of the examination was performed by ophthalmologists. The lens opacities were graded according to a standarized photo of the Lens Opacities Classification System II (LOGS- II ).Results: The prevalence of lens opacities in nuclear color, nuclear opacity, cortical opacity and subcapsular opacity was 1.. 1. , 28.,6% , 30. 3% and 8. 7% , respectively. The frequency of all four types of opacities increased with age. The prevalence of lens opacities in four types was higher in female than that in male. Conclusion: The data we got are important for prevalence of lens opacities of population-based study as well as basic information for

关键词： lens opacities epidemiology

Interaction between CI~- channels and CRAC-related Ca2+signaling during T lymphocyte activation and proliferation

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Acta Pharmacologica Sinica 2006年第4期 437-446页

作者： Guan-lei WANG Yan QIAN Qin-ying QIU Xiu-jian LAN Hua HE Yong-yuan GUAN~2 department of Pharmacology,Zhongshan Medical College,{1.,guangzhou 51.089.China

Aim:To test the hypothesis that Cl~- channel blockers affect T cell proliferationthrough Ca2+-release-activated Ca2+(CRAC)signaling and examine the effects ofthe combination of a CRAC channel blocker and a Cl~- channel blocker on con-canavalin A(ConA;5 mg/mL)-induced Ca2+signaling,gene expression and cellu-lar proliferation in human peripheral T ***:[~3H]Thymidineincorporation,Fura-2 fluorescent probe,RNase protection assay,and reversetranscription-polymerase chain reaction were ***:The Cl~- channelblocker 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid(DIDS)inhibited ConA-induced Ca2+influx,interleukin-2 mRNA expression and T lymphocyte prolifera-tion in a concentration-dependent manner,and also enhanced the inhibitory ef-fects of 1.{beta-[3-(4-methoxyphenyl)propoxyl]-4-methoxyphenethyl}-1.-imida-zole(SK&F96365)on the above key events during T cell activation.A combina-tion of DIDS(1.μmol/L)and SK&F96365(1.μmol/L)significantly diminished ConA-induced ClC-3 mRNA expression by 64%,whereas DIDS(1.μmol/L)or SK&F96365(1.μmol/L)alone decreased ConA-induced ClC-3 mRNA expression by only 1.%and 9%,***:These results suggest that there is an interac-tion between CRAC-mediated Ca2+signaling and DIDS-sensitive Cl~- channelsduring ConA-induced T cell activation and ***,the DIDS-sensitive Cl~- channels may be related to the C1.-3 Cl~- channels.

关键词： T lymphocTytes lymphocyte activation chloride channels calcium ion channels interleukin-2

Local Electroretinogram and Pattern Visual Evoked Potential in Maculopathies

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Eye science 1996年第1期16卷 40-45页

作者： De-zheng Wu, Guangwei Luo, Yi HuangZhongshan Ophtalmic Center, sun yat-sen university of Medical {1.s, guangzhou 51.050, China department of Ophthalmology, Xiang Fan Centre Hospital, Hubei 441.21. China Zhongshan Ophtalmic Center Sun Yat-sen University of Medical Sciences Guangzhou China.

Purposes: To observe the characteristics of local electroretinogram (LERG) in normal subjects and patients with maculopathies, and to evaluate the applied worth of LERG and pattern visual evoked potential (PVEP) in maculopathies. Methods: LERGs at 5° and 1.° macular regions were recorded from 27 normal subjects (54 eyes). The factors of age, different eyes and stimulate areas for LERG influence were observed. Meanwhile, the LERG and PVEP were recorded from 25 patients (35 eyes) with maculopathies for making contrast study. Results: In normal subjects, there was no significant influence of age to LERG. As the stimulated areas increased, the a- and b-wave amplitudes of LERG increased. In the patients with maculopathies, the a- and b-wave amplitudes of LERG at 5°, 1.°and 1.° macular regions were significantly lowered and the mean values of P1.latency were prolonged and N1.P1.amplitudes of VEP were lowered, comparing with the control group. In the nearing stimulated area (5°LERG and 1.. 9×1.°PVEP.),

关键词： maculopathy local electroretinogram (LERG) pattern visual evoked potential (PVEP)

小鼠β_2m反义核酸重组荧光蛋白表达载体的构建及表达研究

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上海免疫学杂志 2002年第5期22卷 304-307,343页

作者：孟瑛李树浓黄绍良中山医科大学病理生理学教研室广州510089 中山医科大学孙逸仙纪念医院儿科广州510120

本研究应用分子生物学技术 ,构建小鼠 β2 m反义核酸重组荧光蛋白表达载体pEGFP β2 mAN ,经过酶切、PCR鉴定 ,以及序列分析证实克隆的正确性。然后用脂质体转染NIH3T3细胞 ,经过RT PCR及Westernblot检测 ,观察重组质粒对细胞β2 mmRNA... 详细信息

本研究应用分子生物学技术 ,构建小鼠 β2 m反义核酸重组荧光蛋白表达载体pEGFP β2 mAN ,经过酶切、PCR鉴定 ,以及序列分析证实克隆的正确性。然后用脂质体转染NIH3T3细胞 ,经过RT PCR及Westernblot检测 ,观察重组质粒对细胞β2 mmRNA和蛋白表达的影响 ,同时在荧光显微镜下直接观察细胞转染的结果。结果证明pEGFP β2 mAN已成功导入NIH3T3细胞中 ,且有效表达 ,在荧光显微镜下可见梭形绿色荧光细胞 ;与同步转染的小鼠 β2 m正、反义核酸表达载体pcDNA3 β2 mSN、pcDNA3 β2 mAN的转染结果一起进行分析 ,转染pcDNA3 β2 mSN细胞的 β2 mmRNA和蛋白表达水平升高 ,而转染pcD NA3 β2 mAN和pEGFP β2 mAN的细胞 β2 mmRNA和蛋白表达水平降低。小鼠 β2 m反义核酸重组荧光蛋白表达载体pEGFP β2 mAN的转染结果显示 :它不但可以降低NIH3T3细胞 β2 m基因的表达 ,而且为观察脂质体转染效果提供了直观。

关键词：基因 MHC I类 β2m 反义核酸细胞转染移植排斥

Microtubule stability and MAP1. upregulation control neuritogenesis in CAD cells

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Acta Pharmacologica Sinica 2006年第9期27卷 1119-1126页

作者： Wen LI~(2,5)Jin-tang XIA~3 Yue FENG~(4,5)2 Laboratory of Surgery,The First Affiliated Hospital,sun yat-sen university,{1. 51.080,China 3 department of General Surgery,The First People's Hospital of {1.,{1. 51.1.0,China 4 department of Pharmacology,Emory university School of Medicine,USA Laboratory of Surgery The First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 Chin

Aim:To study the role of microtubule dynamics and microtubule associated pro-tein 1.(MAP1.)in regulation of the neurite extension in CAD catecholaminergicneuronal cell ***:The neuritogenesis of the CAD cells was abolishedby inhibiting microtubule polymerization with nocodazole and by blocking micro-tubule depolymerization with ***1. and tubulin protein expression wasdetected by Western *** staining of tubulins was observedby fluorescent and confocal ***:Microtubule dynamics wasessential for CAD neurite *** analysis revealed that neurite exten-sion was much more sensitive to nocodazole than to taxol,suggesting a func-tional requirement for highly active microtubule assembly.A remarkableupregulation of MAP IB protein was detected during neurite extension accompa-nied with increased microtubule ***:Upregulation of MAP1.leads to the stabilization of newly formed microtubules in the developing neurites,which in turn promotes neurite extension.

关键词： microtubule stability microtubule associated protein 1. neuritogenesis

Oridonin induces apoptosis via PI3K/Akt pathway in cervical carcinoma HeLa cell line

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Acta Pharmacologica Sinica 2007年第11期28卷 1819-1826页

作者： Hong-zhen HU~2,Yue-bo YANG~3,Xiang-dong XU~4,Hong-wei SHEN~5,Yi-min SHU~6,Zi REN~7,Xiao-mao LI~3,Hui-ming SHEN~3,Hai-tao ZENG~(3,8)~2 department of Gynecology and Obstetrics,Yijishan Hospital,Wannan Medical College,Wuhu 241001,China ~3 department of Gynecologyand Obstetrics,the Third Affiliated Hospital,sun yat-sen university,guangzhou 510080,China ~4 department of Surgery,the First AffiliatedHospital,sun yat-sen university,guangzhou 510080,China ~2 department of Gynecology and Obstetrics,the First Affiliated Hospital,sunyat-sen university,guangzhou 510080.China ~6 department of Obstetrics and Gynecology,IVF Program,Stanford university MedicalCenter,Palo Alto,CA 94304,USA ~7 department of Gynecology and Obstetrics,Guangdong Provincial People's Hospital,guangzhou510080.China ment of Gynecology and Obstetrics Yijishan HospitalWannan Medical College Wuhu 241001 China. zenghaitao@***.

Aim:To investigate the apoptosis-inducing effect of oridonin,a diterpenoid iso-lated from Rabdosia rubescens,in the human cervical carcinoma HeLa cell ***:A morphological analysis,nuclear condensation,and fragmentation ofchromatin were monitored using Hoechst 33342 *** viability was as-sessed using the 3-(4,5-dimethylthiazol-(2)-yl)-2,5-diphenyl tetrazolium bromide(MTT) *** apoptosis and the apoptosis-related activation in the HeLa cellline were evaluated by flow cytometry and Western ***:Oridoninsuppressed the proliferation of the HeLa cell line in a dose-and *** treatment downregulated the activation of protein kinase B(Akt),the expression of forkhead box class O (FOXO) transcription factor,andglycogen synthase kinase 3 (GSK3).Oridonin also induced the release of cyto-chrome c accompanied by the activation of caspase-3 and poly-adenosine diphos-phate-ribose polymerase *** addition,Z-D(OMe)-E(OMe)-V-D(OMe)-FMK (z-DEVD-fmk),an inhibitor of caspases,prevented caspase-3 activation andabrogated oridonin-induced cell ***,oridonin treatment of the HeLa cellline downregulated the expression of the inhibitor of the apoptosis ***:Our results showed that oridonin-induced apoptosis involved sev-eral molecular *** may suppress constitutively activated targetsof phosphatidylinositol 3-kinase (Akt,FOXO,and GSK3) in the HeLa cell line,inhibiting the proliferation and induction of caspase-dependent apoptosis.

关键词： cervical carcinoma oridonin apoptosis Akt

Modeling for cell crowding effects on the adhesion of flowing cells

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Modeling for cell crowding effects on the adhesion of flowin...