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Normality and shared values concerning differential polynomials

中国蚕学会第六届青年学术研讨会

作者： DENG Xiao-Juan 1,2,YANG Wan-Ying 2,HUANG Ya-Dong3,CAO Yang2,WEN Shuo-Yang 4,XU Pei-Lin 1,XIA Qing-You 5 (1 The Key Laboratory of Gene Engineering of Ministry of Education,Sun Yat-sen university,guangzhou 510275,china 2 department of sericulture science,college of animal science,south china agricultural university,guangzhou 510642,china 3 Biopharmaceutical Research and Development Center,Jinan university,guangzhou 510632,china 4 department of Entomology,college of Natural Resource and Environment,south china agricultural university,guangzhou 510642,china 5 Key Sericultural Laboratory of Agriculture Ministry,college of sericulture and Biotechnology,southwest university,Chongqing 400716,china)

Drosomycin（Drs） encodes an inducible 44-residue of antifungal peptide in Drosophila melanogaster. Additional six genes,Dro1,Dro2,Dor3,Dro4,Dro5 and Dro6,are clustered with Drs into a multi-gene family on the 3L chromosome arm. To understand how does each gene of the family respond to microbial infection,we investigated the expression pattern of the drosomycin gene family by microbe-free injury and the microbial challenge. Among these seven genes,Drs,Dro2,Dro3,Dro4 and Dro5,were constitutionally expressed. Dro2,Dro3 and Dro5 were up-regulated by the simple injury. Only the Drs was strongly up-regulated by infection of microbes. Nevertheless Dro1 and Dro6 were not transcribed at all. Furthermore,two transcription start sites of Drs and Dro2,each of Dro3,Dro4 and Dro5,were identified by rapid amplification of cDNA ends. The presence of the NF-κB binding sites in regulatory region of Drs,Dro2,Dro3 and Dro5 genes also indicated the importance of these sites for the inducibility of Drs,Dro2,Dro3 and Dro5 gene. Taking the nested phylogenic information of ORFs into account,we suggested that genes of the drosomycin family have evolved into quite divergent against various stimulations during three duplication events.

关键词： Drosomycins Antifungal peptide Transcription Regulatory motifs Drosophila melanogaster

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science china Mathematics 2010年第3期53卷 749-754页

作者： LEI ChunLin 1 & FANG MingLiang 2, 1 department of Applied Mathematics, south china agricultural university, guangzhou 510642, china 2 Institute of Applied Mathematics, south china agricultural university, guangzhou 510642, china 1. department of Applied Mathematics South China Agricultural University Guangzhou 510642 China2. Institute of Applied Mathematics South China Agricultural University Guangzhou 510642 China

Let F be a family of functions meromorphic in a domain D, let P be a polynomial with either deg P≥3 or deg P = 2 and P having only one distinct zero, and let b be a finite nonzero complex number. If, each pair of fun... 详细信息

关键词： meromorphic function normality shared value

Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis

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Progress in Natural science:Materials International 2008年第7期18卷 819-824页

作者： Junfang Lin a,b, Mingyao Zheng a, Jie Wang a,b, Wei Shu c, Liqiong Guo a,b,* a department of Biotechnology, college of Food sciences, south china agricultural university, guangzhou 510640, china b Institute of Biomass Research, south china agricultural university, guangzhou 510640, china c Test and Experimental Center, south china agricultural university, guangzhou 510640, china department of Biotechnology College of Food Sciences South China Agricultural University Guangzhou 510640 China Institute of Biomass Research South China Agricultural University Guangzhou 510640 China Test and Experimental Center South China Agricultural University Guangzhou 510640 China

An efficient transformation method mediated by PEG-protoplasts was developed for the newly commercial edible mushroom Pleu- rotus nebrodensis. Two plasmids were used to co-transform protoplasts of P. nebrodensis. One plasmid is pAN7-1 containing a positive selectable marker gene hph conferring hygromycin B resistance. Another plasmid is pBlue-GFP containing a reporter gene gfp conferring green fluorescent protein. PCR and southern blot analysis showed that hph gene or/and gfp gene were integrated into the genome of P. nebrodensis transformants. The transformation efficiency of the positive selectable marker gene hph was 3 transformants per microgram of plasmid pAN7-1 DNA, which was about 30 times higher than that previously reported in thoroughly studied Pleurotus species such as Pleurotus ostreatus. The transformation efficiency of the reporter gene gfp was 9 transformants per microgram of plasmid pBlu-GFP DNA. The co-transformation efficiency was 23.68%. This is the first report that a "reporter" gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species.

关键词： Pleurotus nebrodensis Protoplast-mediated transformation Green fluorescent protein GFP expression

H~m convergence rates of solutions of GBBM equations in multi-dimensional spaces

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Progress in Natural science:Materials International 2009年第9期19卷 1053-1057页

作者： Shaomei Fang a,*, Boling Guo b a department of Mathematics, south china agricultural university, guangzhou 510642, china b Institute of Applied Physics and Computational Mathematics, Beijing 100088, china [a]department of Mathematics South China Agricultural University Guangzhou 510642 China [b]Institute of Applied Physics and Computational Mathematics Beijing 100088 China

In this paper, we study the decay rates of the generalized Benjamin-Bona-Mahony equations in n-dimensional space. By using Fourier analysis for long wave and by applying the energy method for short wave, we obtain the Hm convergence rates of the solutions when the initial data are in the bounded subset of the phase space HmeRnTen P 3T. The optimal decay rates are obtained in our results and are found to be the same as the Heat equation.

关键词： Generalized Benjamin–Bona–Mahony equations Fourier analysis Energy method Optimal convergence rates

Effects and Mechanisms of P and K Nutrients on Yield and Protein Content of Fodder Rice

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agricultural sciences in china 2002年第4期1卷 432-437页

作者： TANG Xiang-ru and YU Tie-qiao( department of Agronomy, south china agricultural university, guangzhou 510642 , P.R. china college of Plant science and Technology, Hunan agricultural university, Changsha 410128 , P.R. china) department of Agronomy South China Agricultural University Guangzhou 510642 P. R. China college of Plant science and Technology Hunan Agricultural University Changsha 410128 P.R. China college of Plant science and Technology Hunan Agricultural University Changsha 410128 P.R. China

Effects and mechanisms of P and K nutrients on yield and protein content of Weiyou 56, a fodder hybrid rice combination,were studied through pot experiment and biochemical analysis. The results showed that the increase of P and K nutrients enhanced the activities of PEP carboxylase (PEPC), glutamine synthase (GS) and sucrose phosphate synthase (SPS) in leaves, sucrose synthase (SS), ADP glucose pyrophosphorylase (ADPGP) and GS in grains, and the chlorophyll content in leaves, soluble sugar and starch content in grains, protein N and total N content in leaves and grains. Howerer, they decreased soluble sugar content in leaves and led to an increase of protein content in brown rice, biomass, grain yield and harvest index. Excessive P nutrients slightly reduced SPS and ADPG activity in leaves and grains respectively.

关键词： Superphosphate Potassium chloride Fodder rice Yield Protein content Enzyme activity

Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(Life sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of Agriculture and Forestry science,Beijing 100097,china 2 department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3 college of Life science,Capital Normal university,Beijing 100037,china 4 college of Life science,Zhongkai university of Agriculture and Technology,guangzhou 510225,china 5 college of Grassland science,Gansu agricultural university,Lanzhou 730070,china 6 department of Biology,Henan Institute of science and Technology,Xinxiang 453003,china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. college of Life science Capital Normal University Beijing 100037 China 4. college of Life science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. college of Grassland science Gansu Agricultural University Lanzhou 730070 China 6. department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library

Effects of Citral on Aspergillus flavus Spores by Quasi-elastic Light Scattering and Multiplex Microanalysis Techniques

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Acta Biochimica et Biophysica Sinica 2004年第4期36卷 277-283页

作者： Man LUO1,2, Li-Ke JIANG2, Yao-Xiong HUANG3, Ming XIAO4, Bo LI4, and Guo-Lin ZOU1* 1college of Life sciences, Wuhan university, Wuhan 430072, china 2college of Life sciences, Anhui agricultural university, Hefei 230036, china 3 Institute of Biomedical Engineering, Ji’nan university, guangzhou 510632, china 4Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering, the Institute of Biodiversity science, Fudan university, Shanghai 200433, china college of Life sciences Wuhan UniversityWuhan 430072 China college of Life sciences Anhui Agricultural UniversityHefei 230036 China Institute of Biomedical Engineering Ji'nan UniversityGuangzhou 510632 China Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering the Institute of Biodiversity Science Fudan UniversityShanghai 200433 China

Citral refined from Litsea cubeba oil has been found to have a strong influence on fungi, especially Aspergillus flavus. Multiplex microanalysis and quasi-elastic light scattering techniques were applied to study the effects of citral on Aspergillus flavus spores from the levels of membrane, organelle and intracellular macromolecule. It was found that citral injured the wall and the membrane of A. flavus spore, resulting in decrease of its elasticity. After entering the cell, citral not only influenced the genetic expression of mitochondrion reduplication and its morphology, but also changed the aggregation of protein-like macromolecules. As a result, cells, organelles and macromolecules lost their normal structures and functions, eventually leading to the loss of germination ability of A. flavus spores. Since Litsea cubeba oil as food additive and antifungal agent is safe and less poisonous, it is important to elucidate the inhibitory mechanisms of Litsea cubeba oil on the germination ability of A. flavus spore.

关键词： citral Aspergillus flavus spore multiplex microanalysis techniques quasi-elastic light scattering technique

Nitrogen and Phosphorus Runoff Losses from Orchard Soils in south china as Affected by Fertilization Depths and Rates

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Pedosphere 2008年第1期18卷 45-53页

作者： ZENG Shu-Cai SU Zhi-Yao CHEN Bei-Guang WU Qi-Tang OUYANG Ying college of Forestry South China Agricultural University Guangzhou 510642 (China) college of Natural Resources and Environment South China Agricultural University Guangzhou 510642 (China) department of Water Resources St. Johns River Water Management District P.O. Box 1429 Palatka FL 32178-1429 (USA)

Fertilizers are heavily applied in orchards of the hilly and mountainous topography of south china and may increase nutrient loadings to receiving waters.A simple runoff collecting system was used to measure the effects of different fertilization treatments on total N and P concentrations of surface runoff in a Chinese chestnut (Castanea mollissima Blume) orchard in Dongyuan County,Guangdong Province,*** such orchards,fertilizer was typically applied in two short furrows or pits on either side of each *** included three application depths (surface,10cm and 20 cm),and three application rates (low,median and high).Results showed that 90.5% of the runoff water samples had a total N concentration higher than 0.35 mgL^(-1) and 54.2% had a total P concentration higher than 0.1 mgL^(-1).Fertilizer application at all depths and at all but the lowest rate significantly increased total N and P concentrations in runoff *** with chemical compound fertilizer at a soil depth of 20cm produced significantly lower (P20cm and an application rate≤72 kg N ha^(-1) or 119 kg P ha^(-1) for compound organic fertilizer was suggested to substantially reduce N and P runoff losses from hillslope orchards and to protect receiving waters in south china.

关键词： fertilization depth fertilization rate runoff total N total P

The effect of chromium-riched yeast on growth, feed utilization and tissue composition in tilapia Oreochromis niloticus × O. aureus

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The effect of chromium-riched yeast on growth, feed utilizat...