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Science China(Technological Sciences) 2011年第S1期54卷 54-63页

作者： LI RuoNan 1,CHEN QiuWen 1,2 & Duan Chen 1,3 1 research Center for Eco-environmental Science,Chinese Academy of Sciences,Beijing 100085,China 2 China Three Gorges University,Yichang 443002,China 3 Hydraulics department,Changjiang River Scientific research Institute,Wuhan 430010,China 1. research Center for Eco-environmental Science Chinese Academy of Sciences Beijing 100085 China 2. China Three Gorges University Yichang 443002 China 3. Hydraulics department Changjiang River Scientific Research Institute Wuhan 430010 China

Hydropower development changes the river hydrological regime,thereby altering river ecosystem *** important measure for reducing degradation of ecosystem is to determine ecological flow and implement it by reservoir *** paper described a method to calculate river ecological flow based on fish habitat conservation and applied it to Jinping River Bend of Yalong *** chongi was selected as target species,and the fish habitat model coupling with water environmental model was developed according to the relationship between fish and water environment ***,improved hydraulic habitat suitability index(IHHS) and habitat fragment index(HFI) varying with discharge were discussed in this *** area representing average annual flow was taken as a reference,and then ecological hydrographs under different habitat conservation levels were calculated considering season variation and life stages of *** to the natural condition,the model results indicated that the reservoir operation clearly influenced the habitat of the target *** was proposed to ensure flow releases to maintain 70%-90% habitat in section after joint while about 60% before joint.

关键词： river habitat habitat assessment ecological flow ecological hydrograph

Vascular Basement Membrane-derived Multifunctional Peptide,a Novel Inhibitor of Angiogenesis and Tumor Growth

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Acta Biochimica et Biophysica Sinica 2006年第7期38卷 514-521页

作者： Jian-Guo CAO~(1,2)Shu-Ping PENG~1 Li SUN~2 Hui LI~2 Li WANG~2 Han-Wu DENG~(1*) 1 department of Pharmacology,School of pharmaceutical Sciences,Central South University,Changsha 410078,China 2 Cancer research Institute of Nanhua University,Hengyang 421001,China departmentofPharmacology SchoolofPharmaceuticalSciencesCentralSouthUniversityChangsha410078China CancerresearchInstituteofNanhuaUniversity Hengyang421001China

Vascular basement membrane-derived multifunctional peptide(VBMDMP)gene(fusion geneof the human immunoglobulin G3 upper hinge region and two tumstatin-derived fragments)obtained bychemical synthesis was cloned into vector pUC19,and introduced into the expression vector pGEX-4T-1 toconstruct a prokaryotic expression vector *** VBMDMP produced inEscherichia coli has been shown to have significant activity of antitumor growth and antimetastasis inLewis lung carcinoma transplanted into mouse C57B1/*** the present study,we have studied the ability ofrVBMDMP to inhibit endothelial cell tube formation and proliferation,to induce apoptosis in vitro,and tosuppress tumor growth in *** experimental results showed that rVBMDMP potently inhibited prolif-eration of human endothelial(HUVEC-12)cells and human colon cancer(SW480)cells in vitro,with noinhibition of proliferation in Chinese hamster ovary(CHO-K1)*** also significantly inhibitedhuman endothelial cell tube formation and suppressed tumor growth of SW480 cells in a mouse *** results suggest that rVBMDMP is a powerful therapeutic agent for suppressing angiogenesisand tumor growth.

关键词： recombinant vascular basement membrane-derived multifunctional peptide angiogenesis inhibitor therapeutic action

Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis

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Science China Chemistry 2010年第4期53卷 812-819页

作者： LI ZhiYong1, ZHANG QianYun2, ZHAO LiXia1, LI ZhenJia3, HU GuoMao3, LIN Jin-Ming2 & WANG Shan4 1State Key Laboratory of Environmental Chemistry and Ecotoxicology, research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China 2department of Chemistry, Tsinghua University, Beijing 100084, China 3Beijing Chemclin Biotech co., ltd., Beijing Academy of Science and Technology, Beijing 100094, China 4School of Chemistry and Chemical Engineering, Xianyang Normal college, Xianyang 712000, China 1. State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China2. Department of Chemistry Tsinghua University Beijing 100084 China3. Beijing Chemclin Biotech Co. Ltd. Beijing Academy of Science and Technology Beijing 100094 China4. School of Chemistry and Chemical Engineering of Xianyang Normal College Xianyang 712000 China

A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was carried out by a magnetic plate adaptor and the luminol-hydrogen peroxide (H2O2)-HRP system was employed for the chemiluminescence detection. The proposed method combined the advantages of the micro-plate reactor and magnetic particle separation technology with the linear range of 5-250 ng mL·1. The detection limit of CEA was 0.61 ng mL·1. The coefficient of the variation was less than 7% and 13% for intra-assay and inter-assay precision, respectively. compared with the commercial micro-plate chemiluminescent kit, the proposed method showed a good correlation.

关键词： micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker

Mitogen-activated protein kinase-dependent apoptosis in norcan-tharidin-treated A375-S2 cells is proceeded by the activation of protein kinase C

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中华医学杂志(英文版) 2005年第3期000卷 23-28页

作者： AN Wei-wei, WANG Min-wei, Tashiro Shin-ichi, Onodera Satoshi and Ikejima TakashiChina-Japan research Institute of Medical and pharmaceutical Sciences, Shenyang pharmaceutical University, Shenyang 110016, China department of Pharmacology, Shenyang pharmaceutical University, Shenyang 110016, China department of Clinical and Biomedical Sciences, Showa pharmaceutical University, Tokyo 19-8543, Japan China-Japan research Institute of Medical and pharmaceutical Sciences Shenyang Pharmaceutical University Shenyang 110016 China department of Pharmacology Shenyang Pharmaceutical University Shenyang 110016 China department of Clinical and Biomedical Sciences Showa Pharmaceutical University Tokyo 19-8543 Japan

Background We have reported that norcantharidin (NCTD) induces human melanoma A375-S2 cell apoptosis and that the activation of caspase and the mitochondrial pathway are involved in the apoptotic process. This study aimed at investigating the roles of mitogen-activated protein kinase (MAPK) and protein kinase C (PKC) in A375-S2 cell apoptosis induced by NCTD.

关键词： norcantharidin · A375-S2 cells · mitogen-activated protein kinase · protein kinase C · staurosporine

A preliminary study on synaptogenesis between spinal motor neuron and superior cervical ganglion neuron in vitro

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A preliminary study on synaptogenesis between spinal motor n...

Prediction of petroleum exploration risk and subterranean spatial distribution of hydrocarbon accumulations

中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Can WEN~1,Shu-Rong LI~2,Bing-Yin SU~3 1 department of Neurobiology,Third Military Medical University,Chongqing 400038,China 2 department of Pathology,chengdu Medical college,chengdu 610083,China 3 department of Human Anatomy and Histo-embryology,chengdu Medical college,sichuan key laboratory of development and regeneration,chengdu 610083,China

＜正＞Spinal motor neuron shares the same neurotransmitter（ACh） with sympathetic preganglionic *** spinal motoneurons establish synapses with sympathetic postganglionic neurons? In this study,superior cervical ganglion neuron（SCGN） were labeled by Dil,and SMN-SCGN synaptic model were established by ***-culture was double-immunofluorescently labeled by synatophysin（p38） antibody and ChAT antibody to illustrate the synaptogenesis in the presynaptic neurons,followed by quantitative *** blot and RT-PCR were also performed to confirm the changes of p38 content in the *** spontaneous postsynaptic current（mSPC） was recorded in SCGN so as to observe the influence of co-culture and verify the formation of functional *** found:（1） Primary culture of SMN and SCGN achieved satisfactory growth *** SMN culture,neurons were got aging on the 14th day,In contrast,SCGN still kept excellent growth state on the 16th ***-fluorescent staining of NF200,ChAT or GFAP and Hoechst for nucli revealed that neuronal purity was up to 87.8%,with the purity of SMN being 70.9%and purity of SCGN being 95%.（2） In SCGN culture group,neurons could be labelled by Dil on day 1,and the red fluororescence could last many days without obvious negative influence on the survival of SCGN.（3） SMN and SCGN could be co-cultured on the cover-slip that have been microlandized;quantitative studies showed that co-culture groups significantly outnumbered either single culture group in the number of synapse.（4） Results of western blot indicated that the content of p38 was greatly increased in co-culture group compared with SMN culture group.（5） RT-PCR experiment showed that the level of p38 mRNA was significantly higher in co-culture group than either single culture group or the summation of the two groups.（6） On day 4 in vitro,mSPC was detected in SCGN in the co-culture group,while no similar postsynaptic current was detected in mere SCGN culture *** c

关键词： spinal motor neuron superior cervical ganglion neuron synaptogenesis miniature spontaneous postsynaptic current

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Petroleum Science 2011年第1期8卷 17-23页

作者： Xie Hongbing Guo Qiulin Li Feng Li Jianzhong Wu Na Hu Suyun Liang Kun School of Energy Resources China University of GeosciencesBeijing 100083China research Institute of Petroleum Exploration ＆ development PetroChinaBeijing 100083China Science ＆ Technology Management department China National Petroleum CorporationBeijing 100007China

Investigation of spatial distribution of oil and gas resource and accurate prediction of the geographic location of its undiscovered resource is significant for reducing exploration risk and improving exploration benefit.A new method for predicting spatial distribution of oil resource is discussed in this *** consists of prediction of risk probability in petroleum exploration and simulation of hydrocarbon abundance. Exploration risk probability is predicted by multivariate statistics,fuzzy mathematics and information processing techniques.A spatial attribute database for sample wells was set up and the Mahalanobis distance and Fuzzy value of given samples were ***,the Bayesian formula was used to calculate the hydrocarbon-bearing probability at the area of exploration ***,a hydrocarbon probability template is formed and used to forecast the probability of the unknown area. The hydrocarbon abundance is simulated based on Fourier integrals,frequency spectrum synthesis and fractal ***,the fast Fourier transformation（FFT） is used to transform the known hydrocarbon abundance from the spatial domain to the frequency domain,then,frequency spectrum synthesis is used to produce the fractal frequency spectrum,and FFT is applied to get the phase information of hydrocarbon-bearing ***,the frequency spectrum simulation is used to calculate the renewed hydrocarbon abundance in the play. This method is used to predict the abundance and possible locations of the undiscovered petroleum accumulations in the Nanpu Sag of the Bohai Bay Basin,*** prediction results for the well-explored onshore area of the northern Nanpu Sag agree well with the actual *** the less-explored offshore areas in the southern Nanpu Sag,the prediction results suggest high hydrocarbon abundance in Nanpu-1 and Nanpu-2,providing a useful guiding for future exploration.

关键词： Hydrocarbon resources abundance risk forecast Nanpu sag frequency spectrum simulation fractal theory

After the Termination of Foreign Capital's Super National Treatment

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China's Foreign Trade 2010年第23期000卷 30-31页

作者： Lu Zhiming researcher with the development research department of Bank of communications researcher with the development research department of Bank of communications

The 'super national treatment' enjoyed by foreign capital has finally been put to an end,a factor which has undoubtedly been closely related to the development of China's macro-

关键词：国民外资待遇改革开放宏观经济优惠政策中国资本

In situ absorption and metabolism of stilbene glycoside in rat

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Journal of pharmaceutical Analysis 2010年第4期22卷 223-232页

作者： Chun-Ying Wang1,Zi-Jian Zhang2,Li-Hong Gong3,Lan-Tong Zhang11.department of pharmaceutical Analysis,School of Pharmacy,Hebei Medical University,Shijia-zhuang 050017 2.Hebei Traditional Chinese Medical Hospital,Shijiazhuang 050011 3.department of Chemistry,research Institute of Sports Science,Shijiazhuang 050017,China department of pharmaceutical Analysis School of Pharmacy Hebei Medical University Shijiazhuang 050017 China Hebei Traditional Chinese Medical Hospital Shijiazhuang 050011 China department of Chemistry Research Institute of Sports Science Shijiazhuang 050017 China

Stilbene glycoside(TSG)has been shown to have many beneficial *** is therefore essential to understand the absorption and metabolism of TSG in *** determined the recovery of TSG and its metabolites(TSG sulfate/glucuronides)in rat gastric contents,gastric mucosa,portal vein plasma,celiac arterial plasma,bile,and urine after administration of 15 mg of TSG in 0.5 mL physiological saline or incubation for 20 min in situ in the stomach of *** 20 min,(64.0±9.8)% of the administered TSG disappeared from the stomach;later,TSG was recovered in both free and conjugated forms in plasma and bile,but not in *** the other hand,only free TSG was detected in the gastric contents and mucosa;it was also detected in the portal vein plasma as(48.1±3.5)% of the total TSG(all forms of TSG).However,the proportion of free TSG in the celiac arterial plasma and bile decreased to 4%-10%.In addition,the proportion of free TSG to total TSG in the liver microsome incubation mixture after TSG was incubated in liver microsome at 37 ℃ for 30 min was very low [(10.6 ± 2.6)%].These results indicate that TSG could be quickly absorbed from the rat stomach,conjugated in liver and excreted in *** novel information would be helpful for the use of TSG as a beneficial natural product which may improve its proposed efficacy in preventing chronic diseases.

关键词： in situ absorption metabolism stilbene glycoside rat

Enhancing the capacitances of electric double layer capacitors based on carbon nanotube electrodes by carbon dioxide activation and acid oxidization

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Science China(Technological Sciences) 2010年第5期53卷 1234-1239页

作者： LI ChenSha1,2,QIAO YingJie1,WU JianJun2,WANG DaZhi2 & YUAN DianBo2 1 department of Materials Science,Harbin Engineering University,Harbin 150001,China 2 department of Mechanical Engineering,Tsinghua University,Beijing 100084,China 1. department of Materials Science Harbin Engineering University Harbin 150001 China2. Department of Mechanical Engineering Tsinghua University Beijing 100084 China

Polarizable electrodes of electric double layer capacitors(EDLCs) were made from carbon nanotubes(CNTs).Effect of carbon dioxide activation together with acid oxidation for the electrodes on the characteristics and performances of electrodes and EDLCs was *** dioxide activation changed the microstructure of the electrodes,increased the effective surface area of CNTs and optimized the distribution of apertures of the *** oxidization modified the surface characteristics of *** on the polarizable electrodes treated by carbon dioxide activation and acid oxidization,the performances of EDLCs were greatly *** specific capacitance of the electrodes with organic electrolyte was increased from 21.8 F/g to 60.4 F/g.

关键词： carbon nanotube electric double layer capacitor scan electron microscopy

BATE curve in assessment of clinical utility of predictive biomarkers

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Science China Mathematics 2012年第8期55卷 1529-1552页

作者： ZHOU XiaoHua 1,2,3,& MA YunBei 4,5 1 Northwest HSR&D Center of Excellence,VA Puget Sound Health Care System,Seattle,WA 98198,USA 2 department of Biostatistics,University of Washington,Seattle,WA 98198,USA 3 Beijing International Center for Mathematical research,Peking University,Beijing 100871,China 4 department of Operations research and Financial Engineering,Princeton University,Princeton,NJ 08540,USA 5 School of Statisitcs,Southwest University of Finance and Economics,chengdu 611130,China 1. Northwest HSR&D Center of Excellence VA Puget Sound Health Care System Seattle WA 98198 USA2. Department of Biostatistics University of Washington Seattle WA 98198 USA3. Beijing International Center for Mathematical Research Peking University Beijing 100871 China4. Department of Operations Research and Financial Engineering Princeton University Princeton NJ 08540 USA5. School of Statisitcs Southwest University of Finance and Economics Chengdu 611130 China

In this paper, for time-to-event data, we propose a new statistical framework for casual inference in evaluating clinical utility of predictive biomarkers and in selecting an optimal treatment for a particular patient. This new casual framework is based on a new concept, called Biomarker Adjusted Treatment Effect （BATE） curve. The BATE curve can be used for assessing clinical utility of a predictive biomarker, for designing a subsequent confirmation trial, and for guiding clinical practice. We then propose semi-p^rametric methods for estimating the BATE curves of biomarkers and establish asymptotic results of the proposed estimators for the BATE curves. We also conduct extensive simulation studies to evaluate finite-sample properties of the proposed estimation methods. Finally, we illustrate the application of the proposed method in a real-world data set.

关键词： predictive biomarker cutoff points interaction BATE curve time-to-event outcome