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Meta-analysis of the relationship between Epstein-Barr virus infection and clinicopathological features of patients with gastric carcinoma

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science china(Life sciences) 2010年第4期53卷 524-530页

作者： LI ShuYing1,2, DU HaiJun3, WANG Zhan3, ZHOU Ling3, ZHAO XiaoYu1 & ZENG Yi3 1 college of Life sciences, Hebei University, Baoding 071002, china 2 department of pathogenic biology, faculty of biological science, north china coal medical college, tangshan 063000, china 3 National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese CDC), State Key Laboratory for Infectious Disease Prevention and Control (SKLID), Beijing 100052, china 1. college of Life sciences Hebei University Baoding 071002 China2. Department of Pathogenic Biology Faculty of Biological Science North China Coal Medical College Tangshan 063000 China3. National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention (Chinese CDC) State Key Laboratory for Infectious Disease Prevention and Control (SKLID) Beijing 100052 China

Epstein-Barr virus (EBV) infection has been causally associated with occurrence of many malignant neoplasms. EBV-encoded small RNAs (EBERs) have been detected from about 10% of gastric carcinoma tissue cells, suggesting that EBV infection is associated with the development of gastric carcinoma. The present study pooled the data from the papers concerning EBV-related gastric cancers and performed a meta-analysis of 22 research papers. Among these papers, a total of 5475 cases with gastric cancer were enrolled, of whom 411 cases were found EBV-positive, with the EBV-positive rate being 7.5%. Among the EBV-positive gastric cancer cases, the detection rate was 11.1% in males and 3.0% in females. Compared with EBV-negative gastric cancer, EBV-positive gastric cancer had less lymph node metastasis. Based on the histological typing, of the EBV-positive gastric cancers, the diffuse type was 8.1%, and intestinal type was 8.0%. The examined specimen types included stored paraffin blocks and fresh surgically removed specimens, their EBV positive rates were 7.9% and 6.5% respectively. In terms of geographical distribution, the detection rate of EBV-positive gastric cancer was 9.4% in America, 6.1% in Asia and 9.1% in Europe. Meta-analysis showed that EBV infection occurred only in gastric cancer tissue cells and was significantly associated with the patients’ gender, lymph node metastases, and the location where tumor tissue generated and geographical distribution (P0.05). These results suggested that EBV-positive gastric cancer has distinct clinicopathological features.

关键词： Epstein-Barr virus gastric carcinoma meta-analysis in situ hybridization

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Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

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Progress in Natural science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, SUI Senfang and YANG Mengsu (State Key Laboratory of Biomembrane, department of biological sciences and Biotechnology, Tsinghua University, Beijing 100084, china department of Chemistry, college of Chemistry & Environmental science, Hebei University, Baoding 071002, china department of biology and Chemistry, City University of Hong Kong, Hong Kong, china) State Key Laboratory of Biomembrane Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China. department of Chemistry College of Chemistry & Environmental Science Hebei University Baoding 071002 China. department of biology and Chemistry City University of Hong Kong Hong Kong China

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

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Tissue-specific expression of GFP reporter gene in germline driven by GATA-2 promoter and enhancers in zebrafish

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Chinese science Bulletin 2000年第1期45卷 31-34,97页

作者： MENG Anming & LIN Shuo1. Laboratory of Developmental biology, department of biological sciences and Biotechnology, Tsinghua University, Beijing 100084, china 2. Institute of Molecular Medicine and Genetics, medical college of Georgia, Augusta, GA 30912, USA Laboratory of Developmental biology Department of Biological Sciences and Biotechnology Tsinghua University Beijing China Institute of Molecular Medicine and Genetics Medical College of Georgia Augusta USA

GATA-2, a transcription factor, is expressed in several types of blood cells and in the central nervous system (CNS), and regulates the differentiation of these cells. We have obtained five zebrafish transgenic germlines that carry and express the green fluorescent protein (GFP) gene ligated to various 5’ flanking sepuences of zebrafish GATA-2 gene. The spatial pattern of GFP expression varies, mainly depending on which regulatory sequence is used, among the germlines. In some of the germlines, the expression of GFP is restricted to the CNS and the enveloping layer (EVL) cells, while in some other lines GFP is observed only in the CNS. it is noted that the intensity of GFP in the transgenic fish remain unchanged after a six-generation passage of the transgenes. The transgenic fish could find its uses in the future in generating tissue-specific, even cellspecific mutant fish and in functional study of related genes through transgenesis.

关键词： zebrafish transgenic GATA-2 GFP germline.

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Metallomics:An integrated biometal science

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science china Chemistry 2009年第12期52卷 2055-2070页

作者： GE RuiGuang1,2 & SUN HongZhe1 1 department of Chemistry and Open Laboratory of Chemical biology,The University of Hong Kong,Hong Kong,china 2 Laboratory of Integrative biology,college of Life sciences,Sun Yat-Sen University,Guangzhou 510006,china department of Chemistry and Open Laboratory of Chemical biology The University of Hong Kong Hong Kong China Laboratory of Integrative biology College of Life Sciences Sun Yat-Sen University Guangzhou 510006 China

Metallomics is an emerging scientific area integrating the research fields related to the understanding of the molecular mechanisms of metal-associated life processes and the entirety of metal and metalloid species within a cell or tissue type. In metallomics,metalloproteins,metalloenzymes and other metal-containing biomolecules in a biological system are referred to as metallomes,similar to genomes and proteomes in genomics and proteomics,respectively. This review discusses the concept of metallomics with a focus on analytical techniques and methods,particularly the so-called hyphenated techniques which combine a high-resolution separation technique (gel electrophoresis/laser ablation,chromatography or capillary electrophoresis) with a highly sensitive detection method such as elemental (inductively coupled plasma,ICP) or molecular (electron spray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI)) mass spectrometry,or nuclear X-ray fluorescence/absorption spectrometry. The applications of these advanced analytical methods in the identification of metallo-/phospho-/seleno-proteins,probing of relationships between structure and function of metal-loproteins,and study of clinically used metallodrugs will be selectively outlined,along with their advantages and limitations.

关键词： metallomics metallome metallodrug metalloprotein chemical speciation

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Identification and Characterization of a Rat Novel Gene RSEP4 Expressed Specifically in Central Nervous System

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Acta Biochimica et Biophysica Sinica 2004年第7期36卷 501-507页

作者： Xi-Dao WANG1,2, Ling-Wei KONG1, Zhi-Qin XIE1, Yu-Qiu ZHANG3, Zhi-Xin LIN2, Zhi-Qi ZHAO3,Lei YU4, and Nai-He JING1* 1 Laboratory of Molecular Cell biology, Laboratory of Stem Cell biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, Shanghai 200031, china School of Life science and Biotechnology, 2 Shanghai Jiaotong University, Shanghai 200030, china Institute of Neurobiology, Fudan University, Shanghai 200433, china 3 4 department of Cell biology, Neurobiology and Anatomy, college of Medicine, University of Cincinnati, Cincinnati, Ohio, USA Laboratory of Molecular Cell biology and Stem Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai China

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

关键词： central nervous system (CNS) rat northern blot in situ hybridization

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Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica(Phaeophyta)

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Acta Oceanologica Sinica 2012年第6期31卷 139-148页

作者： XUAN Jinsong 1,4,FENG Yanbin 1,WENG Manli 1,ZHAO Ge 1,SHI Jinfeng 1,YAO Jianting 2,WANG Xiuliang 2,GUO Baotai 3,QIAO Lixian 3,DUAN Delin 2,WANG Bin 1 1 The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental biology,Chinese Academy of sciences,Beijing 100101,china 2 The State Key Laboratory of Marine biology,Institute of Oceanology,Chinese Academy of sciences,Qingdao 266071,china 3 college of Life sciences,Qingdao Agricultural University,Qingdao 266109,china 4 department of biological science and Engineering,School of Chemical and biological Engineering,University of science and Technology Beijing,Beijing 100083,china 1. The State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing 100101 China 4. department of biological science and Engineering School of Chemical and Biological Engineering University of Science and Technology Beijing Beijing 100083 China 2. The State Key Laboratory of Marine biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China 3. college of Life sciences Qingdao Agricultural University Qingdao 266109 China

A high quality cDNA library was constructed from the brown alga Laminaria japonica,with the titer of 1.2×10 5 pfu/*** average insert size of the cDNA library is about 1.6 *** the cDNA library,591 cDNA clones were randomly selected and *** a result,574 EST(expressed sequence tag) sequences were *** of the 574 ESTs were submitted to the dbEST database section of GenBank with the accession numbers from CX942625 to *** cDNA library was screened with a α-32 p labeled 453 bp T P S gene probe,which is a partial sequence yielded from Porphyra *** positive cDNA clones were screened and the sequencing data showed that these four cDNA clones covered majority of *** TPS cDNA *** PCR amplification,sequencing and assembling,the entire ORF(open reading frame) sequence of the T P S gene was obtained,which was named *** encodes a protein containing 908 amino acids with a calculated molecular mass of 101 674 *** LjTPS gene was successfully expressed in *** and *** LjTPS gene has potential application both in plant breeding to stress tolerance and in deciphering the T P S gene function and mechanism to stress tolerance.

关键词： cDNA library comparative study expressed sequence tag(EST) analysis Laminaria japonica trehalose-6-phosphate synthase(TPS) T P S gene

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Immunization of mice with cells from juvenile worms of Schistosoma japonicum provides immunoprotection against schistosomiasis

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science china(Life sciences) 2007年第6期50卷 822-830页

作者： ZENG TieBing1,2, CAI LiTing1, ZENG QingRen1, YANG ShengHui1, YU Rong1, LI YanQin1, FANG HuiLong1, LI BenWen3 & ZHANG ShunKe1 1 Cell & Molecular biological Experiment Center and department of Parasitology, Xiangya School of Medicine, Central South Uni- versity, Changsha 410013, china 2 Institute of pathogenic biology, medical college, Nanhua University, Hengyang 420001, china 3 Infectious Diseases Division, Washington University School of Medicine, Washington 63110, USA Cell & Molecular biological Experiment Center department of Parasitology Xiangya School of Medicine Central South University Changsha 410013 China Institute of pathogenic biology Medical College Nanhua University Hengyang 420001 China Infectious Diseases Division Washington University School of Medicine Washington 63110 USA

To validate the protective efficacy against schistosomiasis by immunization with cells from juvenile Schistosoma japonicum in a murine model; to analyze possible factors related to protection, in this study, two independent repeated vaccination trials were performed. After three subcutaneous vaccinations, in trial one, in the absence of adjuvant, primary juvenile worm cells (pJCs) from S. japonicum induced remarkable average reductions in worm burden (54.3%), liver eggs per gram (LEPG) load (59.8%) as well as egg granulomas size (66.5%) compared to PBS control group (P2 type of immune response while cACs elicited a Th2-type response. Our data indicated that immunization with both primary; cultured cells from S. japonicum juvenile worms provided high immunoprotection, for which the physical character of immunogens, stage-specific parasite; the type of immune response induced might be responsible, suggesting that vaccination with whole cells from S. japonicum larvae is a promising approach to produce protective immunity against schistosomiasis.

关键词： Schistosoma japonicum, juvenile worm cell, cultured cell, immunoprotection, immune response

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Investigation of HGV and TTV infection in sera and saliva from non-hepatitis patients with oral diseases

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World Journal of Gastroenterology 2002年第5期8卷 857-862页

作者： Jie Yan department of pathogenic biology,college of medical science,Zhejiang University,Hangzhou 310031,Zhejiang Province,china Li-Li Chen department of Stomatology,The Second Affiliated Hospital,college of medical science,Zhejiang University,Hangzhou 310009,Zhejiang Province,china Yong-Liang Lou Xiao-Zhi Zhong Wenzhou medical college,Wenzhou 325000,Zhejiang Province,china department of pathogenic biology College of Medical Science Zhejiang University Hangzhou 310031 Zhejiang Province China. yanchen@***

AIM: To determine the frequencies of HGV and TTV infectionsin serum and saliva samples of non-hepatitis patients withoral diseases in Hangzhou area, and to understand thecorrelation between detected results of HGV RNA and/or TTVDNA in sera and in saliva from the same patientsMETHODS: RT-nested PCR for HGV RNA detection andsemi-nested PCR for nv DNA detection were performed inthe serum and saliva samples from 226 non-hepatitis patientswith oral diseases, and nucleotide sequence ***: Twenty-seven (11.9 %) and 21 (9.3 %) of the226 serum samples were only positive for HGV RNA andlrv DNA, respectively. 10 (4.4 %) and 9 (3.9 %) of the226 saliva samples were only positive for HGV RNA andTTV DNA, respectively. And 7 (3.1%) of the serum samplesand 2 (0.9 %) of the saliva samples showed the positiveamplification results for both HGV RNA and Irv DNA. 12saliva samples from the 34 patients (35.3 %) with HGV orHGV/TTV viremia and 11 saliva samples from the 28 patients(39.3 %) with TTV or HGV/TTV viremia were HGV RNAdetectable, respectively, including two patients positive forboth HGV RNA and TTV DNA in serum and saliva *** saliva samples from the 226 patients were found to beHGV RNA or nv DNA detectable while their serum sampleswere negative for HGV or TTV. Homologies of the nucleotidesequences of HGV and TTV amplification products from theserum and saliva samples of the two patients comparedwith the reported sequences were 88.65-91.49 % and65.32-66.67 %, respectively. In comparison with thenucleotide sequences of amplification products betweenserum and from saliva sample from any one of the twopatients, the homologies were 98.58 % and 99.29 % forHGV, and were 98.65 % and 98.20 % for rTV, ***: Relatively high carrying rates of HGV and/or TTV in the sera of non-hepatitis patients with oral diseasesin Hangzhou area are demonstrated. Parts of the carriersare HGV and/or TTV positive in their saliva. The results ofthis study indicate that dentis

关键词：非肝病口腔患者庚型肝炎血清唾液输血传播病毒肝炎 RT-PCR

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Cell cycle-dependent gene networks relevant to cancer

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Progress in Natural science:Materials International 2008年第8期18卷 945-952页

作者： Yun Xiao a,1, Xia Lia,b,*,1, Yueying Yang a, Yadong Wangc, Shaoqi Rao a,b a college of Bioinformatics science and Technology, and Bio-pharmaceutical Key Laboratory of Heilongjiang Province and State, Harbin medical University, Harbin 150086, china b Biomedical Engineering Institute of Capital medical University, Beijing 10004, china c department of Computer science, Harbin Institute of Technology, Harbin 150080, china college of Bioinformatics science and Technology and Bio-pharmaceutical Key Laboratory of Heilongjiang Province and State Harbin Medical University Harbin 150086 China Biomedical Engineering Institute of Capital medical University Beijing 10004 China department of Computer science Harbin Institute of Technology Harbin 150080 China

The analysis of sophisticated interplays between cell cycle-dependent genes in a disease condition is one of the largely unexplored areas in modern tumor biology research. Many cell cycle-dependent genes are either oncogenes or suppressor genes, or are closely associated with the transition of a cell cycle. However, it is unclear how the complicated relationships between these cell cycle-dependent genes are, especially in cancers. Here, we sought to identify significant expression relationships between cell cycle-dependent genes by analyzing a HeLa microarray dataset using a local alignment algorithm and constructed a gene transcriptional network specific to the cancer by assembling these newly identified gene-gene relationships. We further characterized this global network by partitioning the whole network into several cell cycle phase-specific sub-networks. All generated networks exhibited the power-law node-degree distribution, and the average clustering coefficients of these networks were remarkably higher than those of pure scale-free networks, indicating a property of hierarchical modularity. Based on the known protein-protein interactions and Gene Ontology annotation data, the proteins encoded by cell cycle-dependent interacting genes tended to share the same biological functions or to be involved in the same biological processes, rather than interacting by physical means. Finally, we identified the hub genes related to cancer based on the topological importance that maintain the basic structure of cell cycle-dependent gene networks.

关键词： Cell cycle Microarray HeLa cell Gene network

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Characterization of Function of Three Domains in Dishevelled-1: DEP Domain is Responsible for Membrane Translocation of Dishevelled-1

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Cell Research 2004年第4期14卷 324-330页

作者： Wei Jun PAN, Shu Zhao PANG, Tao HUANG, Hui Yun GUO, Dianqing WU, Lin LI Laboratory of Molecular Cell biology, Institute of Biochemistry and Cell biology, Shanghai Institutes for biological sciences, Chinese Academy of sciences, Graduate School of the Chinese Academy of sciences, 320 Yueyang Rd, Shanghai 200031 college of Life science, Jilin University, Changchun 130061, china. department of Genetics and Developmental biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA. 1Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2college of Life science Jilin University Changchun 130061 China. 1Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031. 2college of Life science Jilin University Changchun 130061 China. 3department of Genetics and Developmental biology University of Connecticut Health Center Farmington Connecticut 06030 USA. 1Laboratory of Molecular Cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences 320 Yueyang Rd Shanghai 200031.

Wnt signaling plays an important role in embryogenesis and tumorgenesis. Although the mechanism about how Wnts transduce their signaling from receptor frizzled (Fz) to cytosol has not been understood, dishevelled (Dvl) protein was considered as the intersection of Wnt signal traffic. In this study, we characterized the function of three domains (DIX, PDZ and DEP) of Dvl-1 in canonical Wnt signal transduction and Dvl-1 membrane translocation. It was found both DIX and DEP domain were sufficient to block Wnt-3a-induced LEF-1 transcriptional activity and free cytosol β-catenin accumulation; whereas PDZ domain and a functional mutant form of DEP domain (DEP-KM) had no effect on canonical Wnt signaling. In addition, when cotransfected with Fz-7, DEP domain, but not DIX, PDZ or DEP-KM, translocated and co-localized with Fz-7 to the plasma membrane, which was similar to Dvl-1. Furthermore, it was DEP domain that could block Fz-7-induced membrane translocation of Dvl-1 via a possible competitive mechanism. Thes

关键词： Wnt frizzled-7 dishevelled DIX PDZ DEP membrane translocation signal transduction

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