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Protein expression profile in the differentiation of rat bone marrow stromal cells into Schwann cell-like cells

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science china(Life sciences) 2009年第3期52卷 267-277页

作者： LI WenTing1,SUN HuaLin2,XU ZengLu1,DING Fei2 & GU XiaoSong2 1 department of anatomy,histology and embryology,peking Union medical College,Institute of basic medical science,Chinese Academy of medical sciences,beijing 100005,china 2 Jiangsu Key Laboratory of Neuroregeneration,Nantong university,Nantong 226001,china department of anatomy Histology and Embryology Peking Union Medical College Institute of Basic Medical Science Chinese Academy of Medical Sciences Beijing China Jiangsu Key Laboratory of Neuroregeneration Nantong University Nantong China

During the last decade,increasing evidence suggested that bone marrow stromal cells(MSCs) have the potential to differentiate into neural *** studies have reported that MSCs showed morphological changes and expressed a limited number of neural proteins under experimental ***,no proteomic studies on MSCs differentiated into Schwann cell-like cells have been *** this study,we isolated MSCs from adult Sprague-Dawley rat femur and tibia bone marrows and induced the cells in vitro under specific *** using two-dimensional gel electrophoresis(2-DE),we compared the protein profiles of MSCs before and after induced *** obtained 792 protein spots in the protein profile by 2-DE,and found that 74 spots changed significantly before and after the differentiation using PDQuest software,with 43 up-regulated and 31 *** analyzed these 74 spots by a matrix assisted laser desorption ionization-time of flight mass spectrometry(MALDI-TOF-MS) and by database searching,and found that they could be grouped into various classes,including cytoskeleton and structure proteins,growth factors,metabolic proteins,chaperone proteins,receptor proteins,cell cycle proteins,calcium binding proteins,and other *** proteins also include neural and glial proteins,such as BDNF,CNTF and *** results may provide valuable proteomic information about the differentiation of MSCs into Schwann cell-like cells.

关键词： one marrow stromal cells(MSCs) differentiation Schwann cell-like cells proteomics

Effect of Combination Regimen of Low-dose Gossypol Acetic Acid with Steroid Hormones on Expression of Protein Kinase C alpha (PKC-α) and Cyclin D1 in Rat Testes

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Journal of Reproduction and Contraception 2012年第4期23卷 199-208页

作者： Jin-hui SHAO Zeng-lu XU Xiao-jing QIAN Fang LIU Hui HUANG department of anatomy Histology and Embryology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College department of histology and embryology School of Medicine Hubei University of Art and Science

Objective To investigate the contraceptive mechanism of combination regimen of low- dose gossypol acetic acid （GA） with steroid hormones [desogestre/ethinylestradiol/ testosterone undeeanote（DSG/EE/TU）]. Methods Adult male rats were randomly divided into four groups. Group GH： rats were fed orally with gossypol acetic acid （GA, 12.5 mg/kg） and desogestrel （DSG, 0.125 mg/kg）/ ethinylestradiol （EE, 0.025 mg/kg）/testosterone undecanoate （TU, 100 mg/kg per day, qd X4 weeks or 10 weeks）; group G： a single dose of GA （12.5 mg/kg per day, qd X 4 weeks or I0 weeks）; group H： the same dosage of DSG/EE/TUas in group GH; group C： rats were treated with vehicle （1% methyl cellulose） as the control. Expression of protein kinase C alpha （PKC-a） and cyclin D1 in rat testes were tested mainly by immunohistochemistry （IHC） and Western blotting. Results IHC results showed that protein PKC- a was expressed mainly in interstitial tissue of testis among seminiferous tubule. The expression of PKC-oc in groups H and GH at week 10 was decreased greatly compared with that in group C. The protein cyclin D1 was expressed mainly in residual body of seminiferous tubule eavosurface and interstitial tissue among seminiferous tubule of testis. Western blotting results showed that the expression of PKC-a in groups H and GH at week 10 was decreased significantly compared with that in group C （P〈0.05）. The expression of cyclin D1 in groups G, H or GH at week 10 rose significantly compared with that in group C （P 〈0.05）.Conclusion The administration of low-dose gossypol acetic acid with steroid hormones for 10 weeks can decrease the expression of PKC-a greatly.

关键词： gossypol steroid hormones protein kinase C alpha (PKC-a) cyclin D 1 rat testes

Antifertility Effects of Orally Administration of Low Dose Gossypol Acetic Acid Combined with Methyltestosterone Plus Ethinyl Estradiol on Male Rat

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Journal of Reproduction and Contraception 2008年第4期19卷 201-210页

作者： Zhanjun YANG Wei-san YE Lan WANG Yan GUO She-pu XUE department of Human anatomy BaoTou Medical College Science and Technology University Inner Mongolia Baotou 014010 China department of histology and embryology School of Basic Medicine Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100005 China

Objective To investigate the feasibility and optimal regimen of orally administration of low dose gossypol acetic acid （GA） combined with methyltestosterone （MT） plus ethinyl estradiol （EE） for contraception in males.Methods Wistar male rats were randomly assigned into four groups, 20 in each group. Animals in group A or B were administered daily with 1% methyl cellulose or GA （12 mg/kg） suspended in 1% methyl cellulose, respectively. Rats in group C or D took firstly GA 12 mg/kg＋MT 20 mg/kg＋EE 0.1 mg/kg or MT 20 mg/kg＋EE 0.1 mg/kg, in asuspension with 1% methyl cellulose, via gastric intubation. After the infertilities were initiated（6 weeks for group C, 8 weeks for group D）, GA was served alone while MT＋EE were withdrawn in rats of groups C and D. The treatment was ceased after 18 weeks and some males from group C were permitted to recover. Fertility testing, 10 males per group, was served for determining infertility or restoration of fertility in treated rats. Examinations of histology and biochemistry in treated rats were used to examine the morphologic influences on sperm, testis, epididymides and viscera, and biochemical changes in blood. The growth and development of F1 generation of the rats would also be tested in a series of behavioral tests.Results Ten rats from group C were infertile at week 6 after treatment, and the fulfilled infertility was maintained with low-dose GA （12 mg/kg） only daily. Six weeks after cessation of treatment, all of treated males recovered their fertility. However, 8 of 10 rats from group D were in sterility at 6th week of treatment and all at 8th week of treatment, but the infertility could not be kept with the similar dose GA alone later on. Moreover, no adverse effects were found in our present experiments.Conclusion Administration of oral low dose GA combined with MT and EE as loading dose could successfully induce infertility in short term, whereafter the efficacy could completely be maintained by similar low dose of

关键词： gossypol methyltestosterone ethinyl estradiol antifertility effect rats

p53基因在人皮肤血管瘤不同时期的表达及意义

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华中科技大学学报（医学版） 2005年第5期34卷 647-649页

作者：杨虹张端莲余瑛刘昱华中科技大学同济医学院人体解剖和组织胚胎学系武汉大学医学院人体解剖与组织胚胎学系武汉430071

目的探讨p53基因在血管瘤发生、发展及退化过程中的表达状况及其意义。方法采用免疫组织化学方法(SP法)检测人皮肤血管瘤增生期、退化期及正常皮肤组织中p53基因的表达水平,并结合第Ⅷ因子相关抗原的免疫组织化学染色证实表达p53基因的... 详细信息

目的探讨p53基因在血管瘤发生、发展及退化过程中的表达状况及其意义。方法采用免疫组织化学方法(SP法)检测人皮肤血管瘤增生期、退化期及正常皮肤组织中p53基因的表达水平,并结合第Ⅷ因子相关抗原的免疫组织化学染色证实表达p53基因的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织p53基因表达的平均吸光度和平均阳性面积。结果增生期血管瘤内皮细胞p53基因表达水平高于退化期,差异有极显著性意义(P0.05)。结论p53基因通过促进血管生成而在血管瘤的形成过程中起一定作用。

关键词：血管瘤 p53基因血管内皮细胞生长因子

停服棉酚后恢复的大鼠精母细胞蛋白质合成的研究

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解剖学报 1991年第3期22卷 274-278页

作者：叶百宽张世馥 C.S.Teng 北京中医学院组织学胚胎学教研室北京100029 中国医学科学院基础医学研究所美国北卡罗来那州立大学

经不同时间(3～12周)给大鼠喂服棉酚后应用~3H-精氨酸参入技术,测定睾丸内恢复的第一世代粗线期精母细胞(RPS)的细胞蛋白和核内碱性蛋白合成量的变化。结果表明,与未服棉酚的对照组粗线期精母细胞(CPS)相比较,RPS的细胞蛋白~3H-精氨酸... 详细信息

经不同时间(3～12周)给大鼠喂服棉酚后应用~3H-精氨酸参入技术,测定睾丸内恢复的第一世代粗线期精母细胞(RPS)的细胞蛋白和核内碱性蛋白合成量的变化。结果表明,与未服棉酚的对照组粗线期精母细胞(CPS)相比较,RPS的细胞蛋白~3H-精氨酸参入量有轻度的抑制,抑制程度从12％到26％;而核碱性蛋白合成活性有明显减少,其减少程度从46％到61％。从正常的粗线期精母细胞提取的碱性蛋白作凝胶电泳分离,表明大部分碱性蛋白是核小体联接蛋白和核心蛋白,即组蛋白H_1、H_(2A)、H_(2B)、H_3和H_4,还有少量精子特异蛋白BP和X_1。喂服棉酚后的RPS,其精子特异蛋白BP和X_1以及核心组蛋白H_(2A)和H_4有明显的减少。这表明棉酚影响了PRS核碱性蛋白与BNA量之间的比例,从而改变了细胞的正常生理功能。本文讨论了这种比例与核小体联接和染色质结构的直接关系。

关键词：棉酚精母细胞蛋白

Neuropeptide S promotes active waking through activation of histaminergic and orexinergic neurons

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The inhibitory effect of botulinum toxin type A on pyloric smooth muscle contractile response to substance P in rats in vitro

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Peng ZHAO,Yu-Feng SHAO,Min ZHANG,Kun FAN,Xiang-Pan KONG,Yi-Ping HOU~* department of Neuroscience,anatomy,histology and embryology,Key Laboratory of Preclinical Study for New Drugs of Gansu Province,school of basic medical sciences,Lanzhou university,Lanzhou 730000,china

＜正＞Objective The effect of neuropeptide S（NPS） on characteristics and mechanisms in the regulation of sleep-waking cycle are unclear,though the initial findings that NPS promotes arousal associated to locomotor activity and a genuine anxiolytic-like action have recently been *** hypothesized that NPS activated posterior hypothalamic arousal systems involved histaminergic（HAergic） neurons in tuberomammilary nucleus（TMN） and orexinergic（OXergic） neurons in perifornical（PeF） and lateral hypothalamic（LH） area,where NPSR mRNA has been reported to be highly expressed,to regulate sleep-wakefulness *** present studies were designed to provide more information regarding the NPS-induced sleep-waking characteristics and potential targets in posterior hypothalamus by analyzing each state across sleep-waking cycle,especially focusing on active waking（aW） and quiet waking（qW） states since the rather unique behavioral profile challenges the common idea that anxiolytics are also sedative or that stimulants are also anxiogenic,during the 24 h period after a range dose of NPS intracerebroventricular （ICV） injection and by observing earlier genes c-fos *** Four stainless-steel screws as electroencephalogram（EEG） electrodes were screwed into the animals skull and three silver wire as electromyogram （EMG） electrodes were inserted into the dorsal cervical neck muscles to monitor the sleep-wake cycle,and the EEG and EMG signals were amplified and digitalized with a resolution of 256 and 128 Hz respectively using a CED 1401 MKⅡ.After sleep-waking recording,c-fos immunereactive（Fos-IR）,histidine decarboxylase（HDC）-IR,orexin-A （OX-A）-IR,and dual labeling of Fos-IR+HDC-IR and Fos-IR+OX-A-IR neurons were respectively labeled to identify the active sites in the brain for the NPS effects on *** The sleep-wake profile after an ICV injection of NPS at 10:00 h was examined,when rats with saline injection spend most of their time in *** given at 0.1 a

关键词： neuropeptide S sleep-wake states EEG power spectra immunohistochemistry histamine orexin

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The inhibitory effect of botulinum toxin type A on pyloric s...

Effect of inhibition of GABA uptake in the tuberomamillary nucleus on sleep-waking states following the ventrolateral preoptic area lesion in rats

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Yu-Feng SHAO,Yan-Feng SONG,Yin-Xiang RENG,Xiang-Wen LIU,Yi-Ping HOU~* department of Neuroscience,anatomy,histology and embryology,Key Laboratory of Preclinical Study for New Drugs of Gansu Province,school of basic medical sciences,Lanzhou university,Lanzhou 730000,china

Objective Our previous studies has demonstrated that botulinum toxin type A(BTX-A)intrasphincteric injection through guide cannula in free move rats dose-dependently caused an inhibition of slow wave in amplitude but not in frequency,a diminishment of spike activity in amplitude and spike burst of pyloric myoelectrical activity, and a reduction of substance P(SP)content and cell number of SP-immunoreactivity in *** data suggest that botulinum toxin may inhibit not only the acetylcholine(ACh)release,but also SP release from the autonomic and enteric nervous terminals in ***,a major excitatory non-cholinergic neurotransmitter,depolarizes the membrane potential and thus induces contraction in gastrointestinal smooth *** is an undecapeptide belonging to the tachykinin family and can induce strong contractions in pylorus via neurokinin 1 receptor(NK1R),the preferred receptor for ***,no experimental evidence that BTX-A inhibits the pyloric contractile response induced by SP release from nervous terminals or SP dosed has been documented until *** aim of this study was to determine the inhibitory effect of BTX-A on pyloric smooth muscle contractile response to substance P in rats in vitro. Methods The effect of BTX-A on contractile tension and frequency of pyloric muscle strips incubated with Krebs bicarbonate buffer with electric field stimulation(EFS,100 V,4 Hz,0.5 ms pulse width duration)were detected after atropine(1 uM)abolishing cholinergic action and compared with the effect of[D-Arg,D-Phe,D-Trp,Leu]-SP (APTL-SP),a NK1R *** pyloric smooth muscle contractile response to SP(1 uM)added at intervals of 30 min under a range dose of BTX-A(from 4 to 10 U/mL)incubation for 4 h was measured,and the distributions of SP and NK1R cells in pylorus were examined by *** BTX-A(10 U/mL)as APTLSP (1μM)further decreased EFS-induced pyloric contractile tension(from 1.524±0.029 to 1.362±0.027,P<0.01) and frequency(from

关键词： botulinum toxin type A substance P NK1-receptor antagonist pyloric smooth muscle electric field stimulation rat

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Effect of inhibition of GABA uptake in the tuberomamillary n...

慢性复合应激对大鼠学习和记忆功能及海马内PKA-C_β表达的影响

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Min ZHANG,Kun FAN,Peng ZHAO,Yu-Feng SHAO,Xiang-Pan KONG,Qiong YAN,Yi-Ping HOU~* department of Neuroscience,anatomy,histology and embryology,Key Laboratory of Preclinical Study for New Drugs of Gansu Province,school of basic medical sciences,Lanzhou university,Lanzhou 730000,china

＜正＞Objective Neurons containing gamma-aminobutyric acid（GABA） play an important role in the regulation from wakefulness to *** evidences have demonstrated that the interaction between GABAergic neurons in ventrolateral preoptic area（VLPO） and histaminergic（HA） neurons in the tuberomammillary nucleus（TMN） play a key role for regulating sleep-wakefulness *** neurons also in the TMN have been recently documented. However,GABAergic neurons in TMN have not received much attention for their role in sleep-wakefulness cycle. The present study was to investigate the effect of inhibition of GABA uptake in the TMN nucleus on sleep-waking states following the VLPO lesion and further understand the role of the GABAergic neurons in the TMN in sleep-wakefulness *** Thirty adult and male SD rats were used in this *** anesthesia,4 EEG screw electrodes were implanted into the skull and 3 flexible EMG wire electrodes were placed into the nuchal muscles. After 24 h basal record of sleep-wake cycle,microliter syringes containing ibotenic acid（IA,Sigma,***,USA） solution（10 nmol/0.5μL/side） were bilaterally injected into the VLPO for specific cells *** VLPO lesion, nipecotic acid（NPA,Sigma,***,USA）,a GABA uptake inhibitor（1 mmol/0.5μL/side）,was bilaterally injected into the TMN at 10:00 or 22:00 h for observing the changes of sleep-waking states in light period（8:00-20:00 h） and dark period（20:00-8:00 h） on day 8,10,12 and 14,*** recording,histological Nissl staining and immunohistochemical Fos and glutamate decarboxylase（GAD） staining were respectively performed for determining the loss number of cell and GABA neurons in the *** immunoreactivity（IR） and histidine decarboxylase （HDC）-IR were performed determining the distributions and morphologic characteristics of GABAergic and HAergic neurons in the ***（1） Effect of VLPO lesion on sleep-waking cycle and cortical EEG *** VLPO lesion,the sleep-wa

关键词： GABAergic neuron GABA uptake inhibitor histaminergic neuron lesion glutamate decarboxylaseimmunoreactivity histidine decarboxylase-immunoreactivity tuberomammillary nucles ventrolateral preoptic area

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华中科技大学学报（医学版） 2005年第3期34卷 257-261页

作者：洪小平刘能保张敏海李晓恒王西明刘少纯刘向前孙臣友关中晖华中科技大学同济医学院基础医学院解剖学系华中科技大学同济医学院基础医学院生物化学与分子生物学系武汉430030 哥伦比亚大学神经生物学与行为学中心美国纽约ny10032

目的研究慢性复合应激对成年雄性大鼠学习记忆功能和海马内蛋白激酶A(PKACβ)表达的影响。方法将成年雄性Wistar大鼠随机分为对照组和应激组,应激组动物每天交替暴露于复合应激原中达6周,并测定应激第3周和第6周时两组动物的血清皮质酮... 详细信息

目的研究慢性复合应激对成年雄性大鼠学习记忆功能和海马内蛋白激酶A(PKACβ)表达的影响。方法将成年雄性Wistar大鼠随机分为对照组和应激组,应激组动物每天交替暴露于复合应激原中达6周,并测定应激第3周和第6周时两组动物的血清皮质酮水平。应激结束后,用Morris水迷宫和Y迷宫测试大鼠空间学习记忆成绩,并采用免疫组织化学方法分析海马各亚区PKACβ的表达。结果应激组大鼠的血清皮质酮水平在第3周明显高于对照组,但在第6周两组差别不明显。在Morris水迷宫测试中,应激组动物寻找隐蔽平台的潜伏期明显短于对照组;Y迷宫测试中,应激组动物寻找安全区的正确率也显著高于对照组。应激组大鼠的海马CA1和CA3区PKACβ的表达水平较对照组明显上调,而齿状回(DG)内PKACβ的表达在两组中无明显差别。结论慢性复合应激可增强大鼠海马依赖的学习记忆能力,PKA可能参与了其增强机制。

关键词：慢性复合应激学习与记忆蛋白激酶A 皮质酮海马