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Construction of AFLP-based genetic linkage maps for the Chinese shrimp Fenneropaeneus chinensis

Chinese science Bulletin 2008年第8期53卷 1205-1216页

作者： TIAN Yi1,2,3,4, KONG Jie1↑ & WANG WeiJi1 1 Yellow Sea fisheries Research Institute, Chinese Academy of Fishery science, Qingdao 266071, china 2 college of life science and technology, dalian fisheries university, dalian 116023, china 3 Institute of Oceanology, Chinese Academy of sciences, Qingdao 266071, china 4 Graduate university of Chinese Academy of sciences, Beijing 100049, china Yellow Sea fisheries Research Institute Chinese Academy of Fishery Science Qingdao China college of life science and technology Dalian Fisheries University Dalian China Institute of Oceanology Chinese Academy of Sciences Qingdao China Graduate university of Chinese Academy of sciences Beijing China

Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in china. A genetic linkage map is essential for improving the efficiency of its breeding by marker-as- sisted selection and identifying commercially important genes. Linkage maps of F. chinensis were constructed with an F2 mapping population (110 progenies) using amplified fragment length polymor- phic (AFLP) marker in this study. Fifty-five AFLP primer combinations produced 532 AFLP markers fitting for map strategy in mapping family. The markers with 3:1 segregating ratios were analyzed using F2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. The maps of male, female and common were constructed. The female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 cM. All mark- ers were linked with the linkage groups. Segregation distortion was observed for 6 of 103 markers in the female map. The average distance between markers was 14.53 cM and ranged from 4.4 to 24.8 cM. The male map included 144 markers that formed 35 linkage groups. Ten markers remained unlinked in male map. Segregation distortion was observed for 7 of 144 markers in the male map. The total dis- tance of male map covered 1617 cM. The average distance between markers was 16.36 cM. The male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in Chinese shrimp. The common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cM. Two markers remained unlinked. No distorted markers of 216 markers were shown in the common map. The distance between markers was 10.42 cM. An average estimated genome size for the Chinese shrimp was 2420 cM, which was consistent with the relative size of the Penaeid genome. The distribution of AFLP markers was relatively even in chromosomes of Chi- nese shrimp maps. The linkage analysis

关键词： AFLP 海洋渔业虾中国

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The single nucleotide polymorphisms of chicken melanocortin-4 receptor(MC4R)gene and their association analysis with carcass traits

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science china(life sciences) 2006年第6期49卷 560-566页

作者： QIU Xuemei1,2,LI Ning1,DENG Xuemei3,ZHAO Xingbo3,MENG Qingyong1 & WANG Xiuli2 1. State Key Laboratory for Agrobiotechnology,china Agricultural university,Beijing 100094,china 2. college of life science and technology,dalian fisheries university,dalian,116023,china 3. college of Animal science and technology,china Agricultural university,Beijing 100094,china State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of life science and technology Dalian Fisheries University Dalian 116023 China State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100094 China college of Animal science and technology China Agricultural University Beijing 100094 China college of life science and technology Dalian Fisheries University Dalian 116023 China

Mutations of the melanocortin-4 receptor (MC4R) gene are associated with the appetite, obesity; growth in pig, mice; human. But little is known about the function of chicken MC4R gene. In this study, F2 chicken resource population derived from broilers crossing to Silky was screened for the polymorphisms of the MC4R gene using PCR-single strand conformation polymorphism (PCR-SSCP); DNA sequencing methods. Four single nucleotide polymorphisms (SNPs) sites were found. The mutation (C→T) in the 5′ regulation region of chicken MC4R gene results in one more NF-E2; cap transcription factor binding sites in the mutation allele than in the wild allele. One missense mutation (G→A) occurs in the coding region (61nt), which changes the glycine to arginine. Moreover, in the coding region there are 2 synonymous mutations, one G→T mutation at 315nt; one C→T mutation at 336nt. Least square analysis of the SNPs; carcass traits showed that BB, DD; FF genotypes are significantly associated with body weight, carcass weight (or half carcass weight),; leg muscle weight (P>0.05 or P>0.01). But no significant association between the genotypes; abdominal fat weight is found. The results present the evidence that the chicken MC4R gene can be selected as the major candidate gene for the carcass traits such as body weight; growth.

关键词： melanocortin-4 receptor, SNPs, chicken, carcass traits.

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牙鲆对海水中铅积累排放规律研究

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海洋学报 2004年第3期26卷 109-114页

作者：赵元凤吕景才吴益春宋晓阳王凡刘长发周永赵冲郑伟农业部海洋水产增养殖学与生物技术重点开放实验室辽宁大连116023 大连水产学院生命科学与技术学院辽宁大连116023 大连水产学院辽宁大连116023

研究了海水中铅在牙鲆内脏、肌肉、鳃组织中吸收、积累和排放规律及海水中总有机碳浓度对铅吸收的影响.结果表明,铅浓度为0 5mg/dm3时,各组织中铅蓄积量随暴露时间增加而增大,第9天均达到吸收平衡,此时铅蓄积量(mg/kg)内脏中的649 8大... 详细信息

研究了海水中铅在牙鲆内脏、肌肉、鳃组织中吸收、积累和排放规律及海水中总有机碳浓度对铅吸收的影响.结果表明,铅浓度为0 5mg/dm3时,各组织中铅蓄积量随暴露时间增加而增大,第9天均达到吸收平衡,此时铅蓄积量(mg/kg)内脏中的649 8大于鳃中的237 4大于肌肉中的59 71.染毒13d后将牙鲆移入清洁海水中排放的结果表明,随排放时间增加各组织中铅蓄积量明显下降,第10天时各组织中铅排出率为内脏团的84 85%大于鳃的61 26%大于肌肉的57 03%.海水中总有机碳浓度对铅蓄积量有明显影响,随着总有机碳浓度升高,各组织中铅蓄积量明显下降,表明海水总有机碳能降低铅的生物有效性.

关键词：铅牙鲆积累排放

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菲律宾蛤仔家系的建立及早期生长发育

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水产学报 2010年第1期34卷 32-40页

作者：闫喜武霍忠明张跃环杨凤张国范大连水产学院生命科学与技术学院中国科学院海洋研究所

采用不平衡巢式设计建立33个菲律宾蛤仔家系(11个父系半同胞家系和33个全同胞家系),并对各家系蛤仔的卵径、受精率、孵化率及生长、存活和变态的相关指标进行了分析。结果表明:各家系蛤仔的卵径、受精率无显著差异(P>0.05,n=90),但孵化... 详细信息

采用不平衡巢式设计建立33个菲律宾蛤仔家系(11个父系半同胞家系和33个全同胞家系),并对各家系蛤仔的卵径、受精率、孵化率及生长、存活和变态的相关指标进行了分析。结果表明:各家系蛤仔的卵径、受精率无显著差异(P>0.05,n=90),但孵化率有明显差异(P2生长最快比生长最慢的F2平均壳长大28.24%,且差异显著(P24%。稚贝期间,40日龄I1生长最快比生长最慢的B3平均壳长大78.29%,且差异显著(P2存活率最高比平均存活率高94.14%,E3存活率最低比平均存活率低72.65%。稚贝期间,40日龄时各家系稚贝的存活率较高,都在85%以上。变态期间,同胞家系出现变态延迟及变态规格小型化现象。附着变态时间从第15天延迟至22天,变态平均规格为(193.18±12.15)μm,K1变态最小规格为(183.00±14.42)μm,以K为父本的3个家系变态规格普遍偏小,表现出父本效应。各家系变态率不同,存在明显差异,E3家系变态率最高为(94.33%±0.58%),B2家系变态率最低为(7.33%±2.08%)。综合33个同胞家系的各项指标,I1早期生长抗逆性状最优,可进一步作为优良品种选育的育种材料。

关键词：菲律宾蛤仔家系生长发育

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Multi-objective dynamic detection of seeds based on machine vision

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Progress in Natural science:Materials International 2007年第2期17卷 217-221页

作者： XUN Yi, ZHANG Junxiong, LI Wei and CAI Weiguo ( college of Engineering, china Agricultural university, Beijing 100083, china college of science, dalian fisheries university, dalian 116023, china) college of Engineering China Agricultural University Beijing 100083 China college of sciences Dalian Fisheries University Dalian 116023 China

An approach to inspecting massive numbers of moving seeds was studied based on the techniques of dynamic inspection and machine vision. A progressive scanning CCD camera with external trigger function was used for real-time capture of dynamic images of seeds. The methods based on R channel of RGB (Red, Green and Blue) and region-dependent segmentation were adopted to reduce the data size of image processing and improve the efficiency of seeds inspection. All the seeds were sorted into four grades according to their morphological characteristics, such as surface area, perimeter, major axis, minor axis, circularity and eccentricity. The detection experiments indicated that the eligible ratio of the classifications was about 81.90% by this real-time inspection system.

关键词： machine vision seed sorting multi-objective morphological parameter.

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Fuzzy Probabilistic Analysis of Seismic Stability of Coastal Embankment

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china Ocean Engineering 1996年第1期11卷 99-105页

作者： He Guangne Yang Bin Wen Nai Professor, dalian university of technology, dalian 116023 Engineer, Design and Research Institute of Shanghai Harbour Engineering, Shanghai 200032 . Lecturer, dalian fisheries college, dalian 116023 dalian university of technology Dalian 116023 Design and Research Institute of Shanghai Harbour Engineering Shanghai 200032 dalian fisheries college Dalian 116023

The stability of slope is affected by a number of factors, some of which have not only random property but also fuzzy characteristic. Therefore, the analysis of slope stability is really an uncertain problem. The customary safety factor does not in reality reflect stability scientifically, quantitatively and practically. In order to obtain more practical results, the slope stability is treated as a fuzzy random event for the evaluation of its fuzzy probability. Finally, the seismic stability of an existing coastal embankment is analyzed by means of the suggested fuzzy probabilistic method. It may be seen that the results of analysis can more fully represent the numerical assessment of the degree of slope seismic stability.

关键词： coastal embankment seismic stability fuzzy probabilistic analysis

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Up-conversion emissions of Er^(3+)-Yb^(3+) codoped Al_2O_3 nanoparticles by the arc discharge synthesis method

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science china(Physics,Mechanics & Astronomy) 2009年第7期52卷 1043-1046页

作者： DONG Bin1, CAO BaoSheng1, FENG ZhiQing1, WANG XingJun2, LI ChengRen3 & HUA RuiNian4 1 School of science, dalian Nationalities university, dalian 116600, china 2 School of Electronics Engineering and Computer science, Peking university, Beijing 100871, china 3 college of Physics and Electronic technology, Liaoning Normal university, dalian 116029, china 4 college of life science, dalian Nationalities university, dalian 116600, china School of science Dalian Nationalities University Dalian China School of Electronics Engineering and Computer science Peking University Beijing China college of Physics and Electronic technology Liaoning Normal University Dalian China college of life science Dalian Nationalities University Dalian China

The Er3+-Yb3+ codoped Al2O3 nanoparticles with an average particle size of about 50 nm have been synthesized by an arc discharge synthesis method. The green and red up-conversion emissions centered at about 526, 547 and 677 nm, corresponding respectively to the 2H11/2→4I15/2, 4S3/2→4I15/2 and 4 F9/2→4I15/2 transitions of Er3+, were detected by a 978-nm semiconductor laser diode excitation. The Annealing has evident effect on the up-conversion emissions of the samples: The red up-conversion emission is noticeable before annealing; however, the green up-conversion emission becomes predominant after annealing. The mixture of (Er,Yb)3Al5O12 and α-(Al,Er,Yb)2O3 phases is more favorable for green up-conversion emissions due to an enhancement of the ESA (I) of 4I11/2+a photon→4F7/2 and ET (III) of 2F5/2(Yb3+)+4I11/2(Er3+)→2F7/2(Yb3+)+4F7/2(Er3+) processes. The two-photon absorption up-conversion process is involved in the green and red up-conversion emissions. The results have proved that arc discharge synthesis is a new promising preparation technology for optical materials.

关键词： up-conversion Er3+-Yb3+ codoped Al2O3 arc discharge synthesis

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Functions for patch test in finite element analysis of the Mindlin plate and the thin cylindrical shell

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science china(Physics,Mechanics & Astronomy) 2009年第5期52卷 762-767页

作者： CHEN WanJi 1,2 ,WANG JinZhi 1,3 &ZHAO Jie 1 1State Key Laboratory for Structural Analysis of Industrial Equipment,dalian university of technology,dalian 116023,china 2Institute for Structural Analysis of Aerocraft,Shenyang Institute of Aeronautical Engineering,Shenyang 110136,china 3 college of science,Institute of dalian Nationalities,dalian 116022,china State Key Laboratory for Structural Analysis of Industrial Equipment Dalian University of Technology Dalian China Institute for Structural Analysis of Aerocraft Shenyang Institute of Aeronautical Engineering Shenyang China college of science Institute of Dalian Nationalities Dalian China

Current patch test for Mindlin plate element only satisfies the zero shear deformation *** patch test of non-zero constant shear for Mindlin plate problem cannot be *** shell element, the patch test does not even *** on the theory of enhanced patch test proposed by Chen W J (2006),the authors proposed the enhanced patch test function for Mindlin plate and thin cylindrical shell *** enhanced patch test function can be used to assess the convergence of the Mindlin plate and cylindrical thin shell elements.

关键词： Mindlin plate thin cylindrical shell finite element enhanced patch test test function

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Nonsingularity in second-order cone programming via the smoothing metric projector

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science china Mathematics 2010年第4期53卷 1025-1038页

作者： WANG Yun 1,& ZHANG LiWei 2 1 college of Information sciences and Engineering,Shandong Agricultural university,Tai’an 271018,china 2 Department of Applied Mathematics,dalian university of technology,dalian 116024,china college of Information sciences and Engineering Shandong Agricultural University Tai’an China Department of Applied Mathematics Dalian University of Technology Dalian China

Based on the differential properties of the smoothing metric projector onto the second-order cone,we prove that,for a locally optimal solution to a nonlinear second-order cone programming problem,the nonsingularity of the Clarke's generalized Jacobian of the smoothing Karush-Kuhn-Tucker system,constructed by the smoothing metric projector,is equivalent to the strong second-order sufficient condition and constraint nondegeneracy,which is in turn equivalent to the strong regularity of the Karush-Kuhn-Tucker ***,this nonsingularity property guarantees the quadratic convergence of the corresponding smoothing Newton method for solving a Karush-Kuhn-Tucker ***,the analysis does not need the strict complementarity condition.

关键词： second-order cone programming problem smoothing metric projector B-subdifferential Clarke’s generalized Jacobian smoothing Newton method

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A New Navigation Strategy in Hypermedia Integral Ware Authoring System

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计算机工程 2002年第S1期28卷 141-144页

作者： FU Xianping1 , JIANG Jianping2(1. college of Computer science and technology, dalian Maritime university, dalian 116026,china 2. college of Information Engineering, dalian Maritime university, dalian 116026,china ) college of Computer science and technology Dalian Maritime University Dalian 116026China college of Information Engineering Dalian Maritime University Dalian 116026China

After analyzing the general navigation mechanisms and discussing the knowledge point card linking model which is the base of the navigation mechanism used in the Hypermedia Integral ware Authoring System(HIAS), the pa... 详细信息

关键词： Hypermedia Integral ware Navigation strategy

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