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A characterization for a graphic sequence to be potentially C_r-graphic

粮食安全与现代农业气象业务发展——2008年全国农业气象学术年会

作者： Kemin Yao~1,Ning Hu~1,Wanlong Chen~1,Renzhong Li~1,Qianhua Yuan~2,Feng Wang~3,Qian Qian~4,Shirong Jia~5 1 college of Applied Meteorology,Nanjing university of Information science and Technology,Nanjing,210044,china 2 college of life science and agriculture,hainan university,haikou 570228,china 3 Rice Research Institute,Guangdong Academy of Agricultural sciences,Guangzhou,510640,china 4 National Rice Research Institute,Chinese Academy of Agricultural sciences,Hangzhou,310006,china 5 Bioteehnology Research Institute,Chinese Academy of Agricultural sciences,Beijing 100081,china.

Both rice pollen flow and gene flow model have been established based on the Gaussian Plume Model,the 3 locations×3 years field experimental data of transgene flow to common rice cultivars(Oryza sativa L.),male sterile(ms) lines and common wild rice(*** L.),and the 32 years historical meteorology data during the rice flowering period collected from 38 meteorological stations in southern china. A concept of gene flow coefficient(GFC) is proposed,that is the ratio of the transgene flow frequency(G%) obtained from field experiments against the aggregated pollen dispersal ratio(P) or frequency(P%) calculated based on the pollen flow model. The maximum distances of gene flow(MDGF) to traditional rice cultivars,ms lines,and common wild rice either at a threshold value of 1.0%or 0.1%are determined. The MDGF and its spatial distribution in southern china show that the gene flow pattern is significantly affected by the monsoon climate,the topography,and the outcrossing ability of recipients. We believe that the information provided in this study will be useful for the risk assessment of transgenic rice in other rice growing regions.

关键词： Transgenic rice Pollen flow model Gene flow coefficient Gene flow model Cultivated rice Male sterile lines Common wild rice.

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science china Mathematics 2010年第11期53卷 2893-2905页

作者： YIN JianHua Department of Mathematics, college of Information science and Technology, hainan university, haikou 570228, china 1. Department of Mathematics College of Information Science and Technology Hainan University Haikou 570228 China

Let r 3, n r and π = (d1, d2, . . . , dn) be a graphic sequence. If there exists a simple graph G on n vertices having degree sequence π such that G contains Cr (a cycle of length r) as a subgraph, then π is said to be potentially Cr-graphic. Li and Yin (2004) posed the following problem: characterize π = (d1, d2, . . . , dn) such that π is potentially Cr-graphic for r 3 and n r. Rao and Rao (1972) and Kundu (1973) answered this problem for the case of n = r. In this paper, this problem is solved completely.

关键词： graph cycle degree sequence

Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape

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Journal of Integrative agriculture 2024年第3期23卷 863-875页

作者： hainan Liu Maosong Pei Charles Ampomah-Dwamena Yaxin Shang Yihe Yu Tonglu Wei Qiaofang Shi Dalong Guo college of Horticulture and Plant Protection Henan University of Science and TechnologyLuoyang 471000China Henan Engineering Technology Research Center of Quality Regulation and Control of Horticultural Plants Luoyang 471000China The New Zealand Institute for Plant&Food Research Limited(PFR) Auckland 1010New Zealand

The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated *** study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.

关键词： grape postharvest softening folic acid alternative splicing Pectinesterase 2 alternative 3'splice site(A3SS)

Effect of D-allethrin on proteinase activity in the midgut of the spider Pardosa pseudoannulata detected by bulk acoustic wave impedance technique

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Progress in Natural science:Materials International 2007年第12期17卷 1521-1526页

作者： Wang Zhi1, Fu Xiuqin2, Rao Liqun1 and Lu Xiangyang1(1. college of Bioscience & Biotechnology, Hunan agriculture university, Changsha 410128, china 2. college of life science, Hunan Normal university, Changsha 410081, china) college of Bioscience & Biotechnology Hunan Agriculture University Changsha 410128 China college of life science Hunan Normal University Changsha 410081 China

The piezoelectric bulk acoustic wave (BAW) impedance analysis method was employed to monitor in situ the proteinase-catalyzed hydrolyzation of casein and the effect of pesticide D-allethrin on the proteinase activity in the midgut of the spider Pardosa pseudoannulata, and the results obtained were verified by ultraviolet spectroscopy. The hydrolyzation was significantly increased in the presence of low-dose D-allethrin, whereas significantly inhibited in the presence of high-dose D-allethrin, and a correlation was found precisely between the enzyme activity reflected by the final frequency shift after the hydrolyzation and the concentration of D-allethrin, with a significant frequency response observed even at a 1.5×10-5 volume ratio of D-allethrin to distilled water. The present work may have presented a novel method feasible for study on the mechanism of excitability of spiders under low-dose D-allethrin pesticide and quick detection of proteinase activity.

关键词： Pardosa pseudoannulata D-allethrin proteinase activity ultraviolet spectroscopy piezoelectric bulk acoustic wave impedance method.

Large-scale information entropy analysis of important sites in mature and precursor miRNA sequences

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science china(life sciences) 2009年第8期52卷 771-779页

作者： DU JiangFeng1,2,WU YongJun2,ZHANG YinXia3,WU Lei1,2,WANG XiaoLong3,TAO ShiHeng1,2 1 Bioinformatics Center,Northwest science and Technology university of agriculture,Yangling 712100,china 2 college of life sciences,Northwest science and Technology university,Yangling 712100,china 3 college of science,Northwest science and Technology university,Yangling 712100,china Bioinformatics Center Northwest Science and Technology University of Agriculture Yangling 712100 China college of life sciences Northwest Science and Technology University Yangling 712100 China college of science Northwest Science and Technology University Yangling 712100 China

In order to find evidence of consistent sequence conservation or the base correlation degree in miRNA,some important sites in the sequences of reported miRNA and their precursors(pre-miRNA) were investigated via information entropy *** different groups of sites were obtained from special locations(head,tail) in miRNAs of different sources according to taxonomy(animal,plant and virus) and then analyzed by measuring the single base information redundancy(D1(L)) and the adjacent base related information redundancy(D2(L)).The results showed that D2(L) has more information than D1(L),though D1(L) changes roughly consistently with D2(L) in each *** pre-miRNAs are more conservative than those belonging to animals or *** addition,U is dominant in most sites compared with other *** was also found that in the middle of several groups,there were sites where miRNAs were cut down from pre-miRNAs by Enzyme Dicer which were significantly *** phenomenon shows that the conservatism is an aspect of the of miRNA and may be involved in the recognition and cutting by the *** results provided another perspective for understanding more about the primary structure of pre-miRNA.

关键词： miRNA conservative information entropy precursor evolution base correlation degree

Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

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Journal of Integrative Plant Biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan Liu Yan Bai Zhisong Xu hainan Zhao Haiming Zhao Jinsheng Lai Ya Liu Weibin Song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service Center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

Singularity Detecting with the SecondGeneration Wavelet

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Wuhan university Journal of Natural sciences 2004年第6期9卷 871-874页

作者： XU Bing-lian 1,2 , YI Xu-ming 1 1.School of Mathematics and Statistics, Wuhan university, Wuhan 430072, Hubei, china 2.college of science,Nanjing agriculture university,Nanjing 210095,Jingsu,china School of Mathematics and Statistics Wuhan University Wuhan 430072 Hubei China

Adopting the lifting scheme, introduced by Sweldens, a new wavelet is constructed. It overcomes the shortcoming of the classical wavelets that has no flexibility in the number of vanishing moment. Two examples are given to prove the second-generation wavelets's potentialities in the singularity detection of signal: a wavelet with vanishing moment and symmetry can be constructed according to the problem. Key words biorthogonal wavelet - lifting scheme - secondgeneration wavelets - singularity CLC number O 174.2 Foundation item: Supported by the National Natural science Foundation of china (19602014)Biography: XU Bing-lian (1977-), female, Master candidate, research direction: the wavelets application.

关键词： biorthogonal wavelet lifting scheme secondgeneration wavelets singularity

New procedure for the differential display of mitochondria RNA

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Chinese science Bulletin 1999年第16期44卷 1502-1505页

作者： LING Xingyuan, ZHOU Peijiang and ZHU Yingguo1. Institute of Genetics , college of life science , Wuhan university , Wuhan 430072, china 2. The Center of Biotechnology , Anhui agriculture university , Hefei 230036, china Corresponding author Institute of Genetics College of Life Science Wuhan University Wuhan China The Center of Biotechnology Anhui Agriculture University Hefei China

A novel approach for the differential display of mitochondria RNA has been described . The total mitochondria RNAs are ligated to a single-strand linker with T4 RNA ligase and then reversely transcripted into the first strands of cDNA. The first strands of cDNA that are ligated to another single-strand linker also with T4 RNA ligase can be directly used as the tem-

关键词： rice mitochondria RNA. mRNA differential display. amplification fragment length polymorphism (AFLP ) .

Construction and characterization of the transformation-competent artificial chromosome (TAC) libraries of Leymus multicaulis

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science china(life sciences) 2008年第7期51卷 604-613页

作者： XU YueYu 1,2,3,ZHOU YuLei 4,5 ,SONG LinLin 6 ,ZHANG Yan 3 &ZHAO MaoLin 1 1 Beijing Agro-Biotechnology Research Center,Beijing Academy of agriculture and Forestry science,Beijing 100097,china 2 Department of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,china 3 college of life science,Capital Normal university,Beijing 100037,china 4 college of life science,Zhongkai university of agriculture and Technology,Guangzhou 510225,china 5 college of Grassland science,Gansu Agricultural university,Lanzhou 730070,china 6 Department of Biology,Henan Institute of science and Technology,Xinxiang 453003,china 1. Beijing Agro-Biotechnology Research Center Beijing Academy of Agriculture and Forestry Science Beijing 100097 China 2. Department of Chemistry and Chemical Engineering Hunan Institute of Engineering Xiangtan 411104 China 3. college of life science Capital Normal University Beijing 100037 China 4. college of life science Zhongkai University of Agriculture and Technology Guangzhou 510225 China 5. college of Grassland science Gansu Agricultural University Lanzhou 730070 China 6. Department of Biology Henan Institute of Science and Technology Xinxiang 453003 China

Transformation-competent artificial chromosome system is able to clone and transfer genes efficiently in *** order to clone genes highly tolerant to barley yellow dwarf virus(BYDV),Aphids,drought and salt from Leymus multicaulis,the two TAC genomic libraries I and II were constructed in vector pYLTAC17 and pYLTAC747H/sacB,which contain about 165000 and 236000 recombinant clones *** genome coverage of the two libraries was totally estimated to be about 3―5 haploid genome equivalents,as size selection of genomic DNA fragments was approximately from 9 to 300 *** of the genomic libraries were collected as bulked pools each containing 500 clones or so,stored in twelve 96-deep-well plates and then were gridding in triplicate onto a high-density colony hybridization filter with a 3×3 pattern using a GeneTAC?G3 arraying robot after being transferred manually into three 384-well *** 2501 and 2890 clones of Library in pYLTAC17 and in pYLTAC747H/sacB were stored individually in fourteen 384-well plates and then were automatically gridding in duplicate onto a high-density colony hybridization filter with a 6×6 pattern after a replication of *** positive clones were detected by using the probe glutahione reductase gene of *** libraries constructed here can be used to isolate genomic clones containing target genes,and to carry out genome walking for positional *** the target TAC clones were isolated,they could be immediately transferred into plant genomes with the Agrobacterium system.

关键词： Leymus multicaulis megabase-size DNA transformation-competent artificial chromosome(TAC) genomic library