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Study on low-temperature lipase of psychrophilic bacterium 2-5-1.-1.isolated from deep sea of Southern ocean

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Acta oceanologica Sinica 2003年第4期22卷 643-649页

作者： Lin Xuezheng1.Yang Xiuxia2,Bian Ji1.Huang Xiaohang1.1. First Institute of oceanography,State oceanic Administration,qingdao 266061.china 2. Fishery College, ocean university of china,qingdao 266003,china State ocean Adm Inst Oceanog 1 Qingdao 266061 Peoples R China ocean Univ {1. Fishery Coll Qingdao 266003 Peoples R China

A strain of psychrophilic bacterium, 2-5-1.-1. which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern ocean. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 1. min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2+ and Zn2+.

关键词： Southern ocean deep sea psychrophilic bacteria low-temperature lipase

Annealing effect on InP vertical porous arrays

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Science china(Technological Sciences) 2010年第6期53卷 1562-1566页

作者： SU Ge1.LIU TianZhong2,LIU Wei1.& CAO LiXin1.1.Institute of Material Science and engineering,{1.,qingdao 2661.0,china 2 qingdao Institute of BioEnergy and Bioprocess Technology,Chinese Academy of Sciences,qingdao 2661.1.china 1. Institute of Material Science and engineering Ocean University of China Qingdao 266100 China2. Qingdao Institute of BioEnergy and Bioprocess Technology Chinese Academy of Sciences Qingdao 266101 China

InP vertical porous arrays were produced using electrochemical etching at room *** as-etched InP samples were annealed in an ultra high vacuum ***-sectional analysis of the porous layer was conducted using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX).Annealing in vacuum was found to meliorate the structural quality of the porous *** results showed the composition change of the porous *** controlling the annealing process parameters,the content ratio of phosphorus (P) to indium (In) is *** property of the samples was also investigated at room *** with the sample without annealing treatment,Raman spectrum from the annealed sample showed red-shifted LO and TO peaks together with sharpened LO peak and shortened TO peak.

关键词： InP vertical porous arrays annealing effect Raman property electrochemical etching

Investigation of 'chazi'(damaged-enamels)on Chenghua doucai Porcelains

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Science china(Technological Sciences) 2009年第6期52卷 1722-1729页

作者： DU Feng1.& SU BaoRu2 1.Institute of Chemistry & Molecular engineering,{1. university of Science & Technology,{1.,china 2 College of Information Science & Technology,{1. university of Science & Technology,{1.,china Institute of Chemistry & Molecular engineering Qingdao University of Science & Technology Qingdao China College of Information Science & Technology Qingdao University of Science & Technology Qingdao China

At the end of the 1.50s,Sun Yingzhou considered that the 'chazi'(damaged colour)on Chenghua doucai imperial porcelain as a defect was originally produced in firing process during Chenghua period(1.65―1.87).Researchers in china have accepted his *** any fragment with the 'chazi' has never been found in several tons of Chenghua imperial sherds excavated from Ming imperial kiln site in *** china,few scholars have questioned the formation of 'chazi',and further investigated *** researchers at the Research Laboratory in the British Museum,employing unde-structive EDXRF,have analyzed the chemical composition of the 'chazi'(damaged colour)on a Chen-ghua doucai stem cup in the museum,and they have confirmed that the 'chazi'(damaged colour)was caused by the fire of the Jianfu palace in the Forbidden City in *** on their research,the authors studied the colour chemical principle of the 'chazi's' formation *** to the ceramic technology principle,scientific reasoning for the 'chazi' was carried *** the help of the history of Qing court,the brainteaser of the 'chazi' was totally uncovered,the misunderstanding for the 'chazi' has also been *** research also suggests that employing modern science and technology is a direction to study Chinese ancient ceramic further.

关键词： Chenghua doucai porcelains court eunuchs the set on fire at the Jianfu palace ’chazi’(damaged-enamels) the British Museum colour chemical principle archaeology using science and technology

Glass inclusions in volcanic rocks in the Okinawa Trough back-arc basin: constraints on magma genesis and evolution

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Acta oceanologica Sinica 2001年第3期20卷 383-390页

作者： Yu Zenghui, Zhai Shikui, Li Xianzhong 1. ocean {1. of qingdao, qingdao 266003, china ocean {1. of qingdao Qingdao 266003 China

The major element compositions of glass inclusions in plagioclase and pyroxene phenocrysts of basalt and pumice in the Okinawa Trough back-arc basin are determined by electron microprobe. The results indicate that basalt and pumice are cognate and respectively represent the products at early stages of magmatism and at late stage of crystal fractionation. The initial magma in the trough is rich in H2O. The variation of H2O content in magma may play an important role in the magma evolution. Plagioclase is the mineral crystallized throughout the whole magmatic process and accumulates in the zoned magma chamber. From these features it can be inferred that the initial magma in the Okinawa Trough, whose opening began in recent years, is seriously affected by fluid or other materials carried by subducting slab and the geochemical feature of volcanic rocks is in some degree similar to that of lavas in island-arc environments.

关键词： Glass inclusion magma genesis and evolution Okinawa Trough

Self-assembly structure formation on patterned InP surfaces

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Science china(Technological Sciences) 2009年第9期52卷 2732-2736页

作者： SU Ge1.2,CAO LiXin1.& LIU TianZhong3 1.Institute of Material Science and engineering,{1.,qingdao 2661.0,china 2 Nanoscale Physics Research Laboratory,School of Physics and Astronomy,the university of Birmingham,Edgbaston,Birmingham B1. 2TT,UK 3 College of Food Science and engineering,{1.,qingdao 266003,china Institute of Material Science and engineering Ocean University of China Qingdao 266100 China Nanoscale Physics Research Laboratory School of Physics and Astronomy the University of Birmingham Edgbaston Birmingham B15 2TT UK College of Food Science and engineering Ocean University of China Qingdao 266003 China

Self-assembly of polystyrene spheres guided by patterned n-type InP substrates has been investigated. InP surfaces were patterned using a variety of methods including wet chemical etching,sputter coating,thermal evaporation,and photo lithography. The self-assembly of polystyrene spheres depended on the appearance of patterns and was affected by the deposition techniques (sputter coating and thermal evaporation) of Au micro-squares. SEM and AFM were used to characterize the surface morphologies.

关键词： self-assembly polystyrene spheres pattern InP surface

Modeling of suspended sediment transport with wave-induced longshore current in Huanghe (Yellow) River Delta

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Acta oceanologica Sinica 2009年第3期28卷 65-74页

作者： LI Changliang LIANG Bingchen ZHANG Jing College of Petroleum engineering China University of Petroleum（East China） Qingdao266555 China engineering College Ocean University of China Qingdao 266100 China

A three-dimensional suspended sediment model （SED） developed by the present authors is coupled with the combinatorial model of COHERENS （Luyten et al., 1.99）（the three-dimensional coupled hydrodynamical-ecological model for Regional and Shelf Seas） and SWAN （Holthuijsen et al., 2004）（the third generation wave model）. SWAN is regarded as a subroutine of COHERENS and gets time- and space-varying current velocity and surface elevation from COHERENS. COHERENS gets time- and space- varying wave relevant parameters provided by SWAN. Effects of wave on current are applied in bottom shear stress, wave-induced depth-dependent radiation stress and surface drag coefficient calculation. At the same time, the damping function of suspended sediment on turbulence is introduced into COHERENS. So the sediment model SED has feedback on circulation model COHERENS. The SED obtains current associated parameters from COHERENS. Then a coupled hydrodynamic-sediment model COHERENS-SED being able to account for interaction between wave and current is obtained. COHERENS-SED is adopted to simulate three-dimensional suspended sediment transport in the Huanghe River delta. In terms of simulation results, there is obvious difference between top and bottom layer of wave-induced longshore current. The values of time series of sediment concentration gotten by COHERENS-SED have, generally, an accepted agreement extent with measurement. Significant wave heights and wave periods obtained by COHERENS-SED show that wave simulation case with currentts effect can give better agreement extent with measurement than case without current＇s effect. In the meantime, suspended sediment concentration distributing rule obtained by COHERENS-SED is similar to former researches and measurement.

关键词： Huanghe River delta suspended sediment wave-induced longshore current COHERENS SWAN COHERENS-SED

Physiological and ultralstructural changes of Chlorella sp. induced by UV-B radiation

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Progress in Natural Science:Materials International 2005年第8期15卷 678-683页

作者： YU Juan1. TANG Xuexi1., ZHANG Peiyu 1.2, TIAN Jiyuan1.and DONG Shuanglin1.1. Division of Life Science and Technology, ocean university of china, {1. 266003, china 2. College of Life Sciences, Qufu Normal university, Qufu 2731.53, china) Division of Life Science and Technology Ocean University of China Qingdao 266003 China. College of Life Sciences Qufu Normal University Qufu 2731653 China

In order to investigate the mechanisms of enhanced UV-B radiation on algae, the effects of UV-B radiation on the physiological and ultrastructural changes of Chlorella sp. were examined. The results showed that UV-B radiation could inhibit the growth and photosynthesis of microalgae. UV-B radiation at lower doses increased the photosynthetic pigment (chlorophyll a (Chla) and carotenoid (Car)) contents, while at higher doses of UV-B radiation Chla and Car contents were decreased. The ultrastructure of Chlorella sp. without exposure to UV-B showed that the thylakoid lamellae were clear and regular, the stroma of its chloroplast was apparent and clear. The globules with photosynthetic pigments and the cristae of mitochondria were clearly seen. After exposure to UV-B radiation at dose of 2.88 kJ/m2, the thylakoid lamellae of Chlorella sp. were lost and dissolved, the globules which contained photosynthetic pigments in chloroplast were bleached; some mitochondria cristae were dissolved; slight plasmolysis was found in some Chlorella sp. cells. After exposure to 5.76 kJ/m2 UV-B radiation, the thylakoid was in disarray and disintegration, plasmolysis was found in most cells, and the cell wall was broken and began to fall out. Many blank areas were observed in cells, mitochondria were seriously deformed and most of the mitochondria cristae were dissolved. Also, globules containing photosynthetic pigments in chloroplast were bleached and some empty globules were found in chloroplast. Therefore, UV-B radiation could damage cell structure of Chlorella sp., and this damage increased with the dose of UV-B radiation they exposed to.

关键词： UV-B radiation physiological changes ultrastructure Chlorella sp.

Construction and application of formal ontology for mine

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中国有色金属学会会刊：英文版 2011年第S3期21卷 577-582页

作者： CHENG Gang 1. 2, ZHANG Yin-ling1. 2, WANG Fei 1.,2, ZHANG Zhen-hui 3, GUO Yu-xiang1. 2 1. Key Laboratory of Mine Spatial Information Technologies of State Bureau of Surveying and Mapping, Henan Polytechnic university, Jiaozuo 454003, {1. 2. School of Surveying and land Information engineering, Henan Polytechnic university, Jiaozuo 454003, {1. 3. qingdao Branch of Naval Aeronautical engineering Institute, qingdao 266041. {1. Henan Polytech Univ Key Lab Mine Spatial Informat Technol State Bur Surveying & Mapping Jiaozuo 454003 Peoples R China Henan Polytech Univ Sch Surveying & Land Informat Engn Jiaozuo 454003 Peoples R China Naval Aeronaut Engn Inst Qingdao Branch Qingdao 266041 Peoples R China

Digital mine is the only way for the development of mining industry in china. Due to lack of appropriate standards and norms, and different awareness in the field of digital mine among academia and industry insiders, the meaning for digital mine is still unclear. Starting from the nature of mining and removing of views of specialized fields, this paper constructs formal ontology for digital mine and proposes the four levels for it. The ontology clarifies the concept world for digital mine, defines the meaning of concepts and relations clearly, provides a reference for the standard construction for digital mine and provides a unified semantic framework for the integration of heterogeneous mine data. Meanwhile, it can provide formal reasoning knowledge for expert system of digital mine and improve the intelligence and automation while the machine automatically interpreting and processing mine spatial data.

关键词： Digital mine formalization ontology concept world semantic share

Characteristics of sediment transport in the Zhoushan Archipelago sea area

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Acta oceanologica Sinica 2009年第5期28卷 116-127页

作者： HU Rijun 1.2 , WU Jianzheng 1.2 ? , LI Guangxue 1.2 , ZHU Longhai 1.2 , MA Fang 3 1.College of Marine Geoscience, ocean university of china, {1. 2661.0, china 2 Key Lab of Submarine Geosciences and Prospecting Techniques, Ministry of Education, ocean university of china, {1. 2661.0, china 3 North china Sea Branch of the State oceanic Administration, {1. 266033, china ocean Univ {1. Coll Marine Geosci Qingdao 266100 Peoples R China ocean Univ {1. Key Lab Submarine Geosci & Prospecting Tech Minist Educ Qingdao 266100 Peoples R China State ocean Adm N China Sea Branch Qingdao 266033 Peoples R China

Based on the field data including tidal current, suspended sediment concentration, grain size of surface sediments, the transport mechanism and movement trend of sediments are analyzed using the method of flux decomposition and Grain Size Trend Analysis. The results show that the concentration and flux of suspended sediments decrease sharply from the Hangzhou Bay to the offshore area. The suspended sediment transport is mainly controlled by advection transport including average current transport and Stokes drift-induced transport and the gravitational circulation transport. The surface sediments are transported from the entrance of the Hangzhou Bay to the offshore area in general; meanwhile, the sediment transport has two obvious transport trends in the offshore area. The interaction of tidal currents, residual currents,the East china Sea coastal current, Taiwan Warm Current and wind waves appear to play important roles in the sediment transport. Furthermore, the sediment distribution and transport are significantly affected by the Zhoushan Archipelago.

关键词： the Zhoushan Archipelago suspended sediments surface sediments sediment transport dynamic mechanism