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Changes in Autoantibodies against β1-Adrenoceptor and M2-Muscarinic Receptor during Development of Renovascular Hypertension in Rats

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Acta Biochimica et Biophysica Sinica 2004年第12期36卷 793-797页

作者： Jian-Ming ZHI*, Rong-Fang CHEN1, Heng WU2, Hui-Rong LIU2, and Rong-Rui ZHAO2 department of Physiology, shanghai second medical university, shanghai 200025, china 1department of toxicology, second military medical university, shanghai 2.04.3, china 2 department of Physiology, Shanxi medical university, Taiyuan 030001, china department of Physiology Shanghai Second Medical UniversityShanghai 200025 China department of toxicology Second Military Medical UniversityShanghai 200433 China department of Physiology Shanxi Medical UniversityTaiyuan 030001 China

In an experimental rat’s renovascular hypertension model, we studied the genesis of anti- cardiac β1-adrenoceptor and M2-muscarinic receptor autoantibodies in relation to the changes in immunological function during the development of renal hypertension. The biological activities of these autoantibodies were also examined. It was shown that after two weeks of operation both the frequency of occurrence and the titre of autoantibodies to cardiac β1-adrenoceptor and M2-muscarinic receptor were significantly increased as compared with the control of pre-treatment. The increased autoantibodies lasted for several weeks and then automatically decreased gradually to the pre-clipping level at 10 weeks. Meanwhile the ratio of CD4 /CD8 + + was also undergone an initial increase followed by gradual recovery and correlated well with the changes in antibody titre. The biological effects of these autoantibodies displayed an “agonistic-like” activities on the beating frequency of cultured neonatal cardiomyocyte. It is suggested that autoimmune mechanisms are involved in the pathogenesis of renal hypertension and the cardiac receptor autoantibodies might be one of the mechanisms leading to cardiac dysfunction.

关键词： autoantibody renal hypertension cardiac receptor immunology

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Construction,Expression,and Characterization of a Recombinant Annexin B1-Low Molecular Weight Urokinase Chimera in Escherichia coli

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Acta Biochimica et Biophysica Sinica 2004年第3期36卷 184-190页

作者： Hong-Li YAN, Wei-Ting WANG1, Yan HE , Zhuan-You ZHAO1,Yuan-Jian GAO, Yi ZHANG, and Shu-Han SUN department of medical Genetics, second military medical university, shanghai 2.04.3, china 1department of Pharmacology, Tianjin Institute of Medicine, Tianjin 300193, china department of medical Genetics Second Military Medical University department of Pharmacology Tianjin Institute of Medicine

To produce a thrombi-targeting plasminogen activator, low molecular weight single-chain urokinase gene (scuPA32k) was spliced with the full-length cDNA of annexin B1 gene (anxB1) by overlap extension method. The fused gene anxB1scuPA was ligated into pET28a vector, transformed into E. coli BL21-RIL, and then induced to express under the control of T7 promoter. The AnxB1ScuPA protein ex- pressed amounted to 22% of the total bacterial proteins. The product was refolded, and then purified by using DEAE Sepharose fast flow ion-exchange column and Superdex S-200 gel-filtration column. HPLC analysis revealed that the final purity is about 95%. The specific activity of AnxB1ScuPA, measured as amidolytic activity, reached 100,000 IU/mg. It had a similar S2444 catalytic efficiency (kcat/Km) to ScuPA32k, and also showed high activated-platelet membrane-binding activity and anticoagulant activity, indicating that the chi- mera fully retained the components of enzymatic and membrane-binding activities of the parent molecules. In vivo test revealed that, the dogs administered with AnxB1ScuPA had less reperfusion time, higher reperfu- sion ratio, and less bleeding effects than those with urokinase. These findings indicated that AnxB1ScuPA might have advantages over current available thrombolytic agents.

关键词： annexin B1 thrombolytic agent low molecular weight single-chain urokinase chimera

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CYP2E1 Pst Ⅰ/Rsa Ⅰ polymorphism and colorectal cancer risk:A meta-analysis

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World Journal of Gastroenterology 2010年第23期16卷 2949-2953页

作者： Guo-Wu Zhou,Jia Hu,Qiang Li department of Respiration,Changhai Hospital,second military medical university,shanghai 2.04.3,china department of Oncology,Changhai Hospital,second military medical university,shanghai 2.04.3,china department of Respiration Changhai Hospital Second Military Medical University Shanghai 200433 China.

AIM:To clarify the association between CYP2E1 PstⅠ/RsaⅠ polymorphism and susceptibility to colorectal ***:A meta-analysis based on 10 eligible casecontrol studies involving 4979 cases and 6012 controls was carried out to summarize the data on the association between CYP2E1 RsaⅠ/PstⅠ polymorphism and colorectal cancer ***:In comparison of the homozygote c2c2 and c2 carriers(c1c2 + c2c2) and the homozygous wild-type genotype(c1c1),no association was found between CYP2E1 RsaⅠ/PstⅠ polymorphism and colorectal cancer risk [odds ratio(OR)=1.24(95% CI:0.93-1.66) for c2c2;OR = 1.02(95% CI:0.88-1.19) for c2 carriers].In stratif ied analysis,Caucasians with c2c2 homozygote appeared to have an increased risk of colorectal cancer(OR=2.67,95% CI:1.03-6.89,P=0.043),no signif icant associations were found in other ***:c2c2 homozygote of CYP2E1 PstⅠ/RsaⅠ polymorphism may be associated with the increased risk of colorectal cancer in Caucasians,which needs further investigations.

关键词： Cytochrome P450 2E1 PstⅠ/RsaⅠ polymorphism Colorectal cancer Cancer susceptibility Metaanalysis

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The cell cycle related apoptotic susceptibility to arsenic trioxide is associated with the level of reactive oxygen species

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Cell Research 2004年第1期14卷 81-85页

作者： Fei GAO~1, Jing YI~1, Jing Qi YUAN~2, Gui Ying SHI~1, Xue Ming TANG~(1,*) ~1department of Cell Biology, shanghai second medical university, shanghai 200025, china ~2department of Automation, shanghai Jiaotong university, shanghai 200031, china. Tang XM Shanghai Med Univ 2 Dept Cell Biol Shanghai 200025 Peoples R China

Double staining flow cytometry was performed using 7-amino actinomycin D and 6-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, to detect the level fluctuation of reactive oxygen species （ROS） during the cell cycle of normal NB4 cells. Our results showed that NB4 cells possessed higher level of ROS in G2/M phase than in G1 and S phases. Double staining flow cytometry, with TdT mediated dUTP nick end labeling （Tunel） and propidium iodide （PI）, indicated that As2O3 （2μM） could induce apoptosis in NB4 cells prevailingly from G2/M phase, and this efficacy was enhanced upon co-administration of 2, 3-dimethoxy-1, 4-naphthoquinone （DMNQ）（2.5μM） which could produce the endogenous ROS. These results suggested that different ROS level in different cell cycle phases of NB4 cells might determin the selective induction of G2/M apoptosis and the cells’ susceptibility to apoptosis by As2O3.

关键词： arsenic trioxide apoptosis cell cycle reactive oxygen species (ROS).

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The convergent point of the endocytic and autophagicpathways in leydig cells

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Cell Research 1999年第4期9卷 243-253页

作者： YI JING XUE MING TANG(department of Biophysics and Cell Biology, shanghai second medical university, shanghai 200025, china) department of Biophysics and Cell Biology Shanghai Second Medical University Shanghai 200025 China

INTRODUCTIONEndocytosisandalltophagocytosis(alltophagy)arethemajorpathwaysformaterialstobetransportedtolysosolnesillanimalcells,theformerbeingresponsibleforuptakeofextracellular***... 详细信息

INTRODUCTIONEndocytosisandalltophagocytosis(alltophagy)arethemajorpathwaysformaterialstobetransportedtolysosolnesillanimalcells,theformerbeingresponsibleforuptakeofextracellular***...

关键词： Autophagocytosis endocytosis endosomes lysosomes.

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Complete resection of isolated pancreatic metastatic melanoma:A case report and review of the literature

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World Journal of Gastroenterology 2010年第36期16卷 4621-4624页

作者： Miao-Xia He,Hui Jiang,Jian-Ming Zheng,department of Pathology,Changhai Hospital,second military medical university,shanghai 2.04.3,china Bin Song,Xian-Gui Hu,Yi-Jie Zhang,department of General Surgery,Changhai Hospital,second military medical university,shanghai 2.04.3,china department of Pathology Changhai Hospital Second Military Medical University Shanghai 200433 China. jmzheng1962@***

Isolated metastatic melanoma of the pancreas is very ***,there is very limited experience with surgical resection of pancreatic *** potential benefit of metastasectomy can improve the quality of life and survival time of *** present a case of a 39-year-old Chinese male with a solitary pancreatic tumor which was considered a cystic benign lesion for *** and immunohistochemistry showed that the tumor in pancreatic tail was a metastasis from a malignant melanoma of the *** other metastastic foci were found in *** tumor was completely resected with combined distal pancreatectomy and *** patient has survived 25 mo without any signs of local recurrence or other metastatic lesions after operation,indicating that complete surgical resection of a solitary metastatic melanoma of the pancreas can prolong the survival time of patients.

关键词： Melanoma Metastasis Pancreas Solitary tumor Resection

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Telomere and telomerase in oncology

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Cell Research 2002年第1期12卷 1-7页

作者： JIAO MU, Li XIN WEI International Joint Cancer Institute, second military medical university, shanghai 2.04.3, china International Joint Cancer Institute Second Military Medical University Shanghai 200433 China

Shortening of the telomeric DNA at the chromosome ends is presumed to limit the lifespan of human cells and elicit a signal for the onset of cellular senescence. To continually proliferate across the senescent checkpoint, cells must restore and preserve telomere length. This can be achieved by telomerase, which has the reverse transcriptase activity. Telomerase activity is negative in human normal somatic cells but can be detected in most tumor cells. The enzyme is proposed to be an essential factor in cell immortalization and cancer progression. In this review we discuss the structure and function of telomere and telomerase and their roles in cell immortalization and oncogenesis. Simultaneously the experimental studies of telomerase assays for cancer detection and diagnosis are reviewed. Finally, we discuss the potential use of inhibitors of telomerase in anti-cancer therapy.

关键词： Telomere telomerase cancer telomerase assay inhibitor.

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Apoptosis in oncology

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Cell Research 2001年第1期11卷 1-7页

作者： FAN XIAO QIANG, YA JUN GUO (International Joint Cancer Institute, The second military medical university, shanghai 2.04.3, china) 1International Joint Cancer Institute The Second Military Medical University Shanghai 200433 China 1International Joint Cancer Institute The Second Military Medical University Shanghai 200433 China

Apoptosis is a complex process involving a large array of genes and mutation of any of these genes may lead to malignancy formation. Re-acquirement of FasL by tumor cells may enable them to evade the surveillance of immune system and thus contributes to the growth of tumor. Apart from traditional therapies, inducing apoptosis of tumor cell by new methods employing death receptor ligands and making use of Fas counterattack is also being developed.

关键词： Apoptosis mitochondria death receptor death receptor ligand tumor therapy.

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About the evaluation of drug combination

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Acta Pharmacologica Sinica 2004年第2期 20-21页

作者： Zheng-jun JIN1department of Pharmacology, shanghai second medical university, shanghai 200025, china

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Cloning, Expression, Purification and Crystallization of NHR3 Domain from Acute Myelogenous Leukemia-related Protein AML1-ETO

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 566-570页

作者： Hai-Tao YANG , Dong-Hui WU1 , Xiao-Yu XUE, Wen-Xue LIANG1, Xiao-Yu MIAO,Hai PANG , and Sai-Juan CHEN1Laboratory of Structural Biology, department of Biological Sciences and Biotechnology & Protein Sciences Laboratory of MOE, Tsinghua university, Beijing 100084, china shanghai Institute of Hematology, State Key Laboratory of medical Genomics,1 shanghai second medical university, shanghai 200023, china Laboratory of Structural Biology Department of Biological Sciences and Biotechnology & Protein Sciences Laboratory of MOE Tsinghua University shanghai Institute of Hematology State Key Laboratory of Medical Genomics Shanghai Second Medical University

The t(8;21) translocation is one of the most frequent chromosome abnormalities in acute myeloid leukemia. This translocation creates a fusion between the acute myelogenous leukemia 1 (AML1, a transcription factor) gene on chromosome 21 and the eight-twenty-one (ETO, a zinc finger nuclear protein) gene on chromosome 8, leading to the repression of certain AML1 target genes. We cloned NHR3 domain coding fragment into vector pGEX-6p-1 using PCR and obtained recombinant plasmid pGEX-6p-1-NHR3, which can be induced to stably overexpress fusion protein in E. coli. Through the purification on GST affinity chromatography column and PreScission protease cleavage, a large amount of NHR3 protein with high purity was obtained. In order to avoid possible interference of some strong negative charged molecules, NHR3 protein was further purified by Mono Q anion exchange chromatography. The NHR3 crystals were grown with hanging drop/vapor diffusion method and the first crystals appeared after four weeks at 18 °C in 0.2 M Tris-sodium citrate dihydrate, 0.1 M sodium cacodylate, pH 6.5, and 30% iso-propanol (V/V). ESI mass spectrum showed that the molecular weight of this domain was in agreement with its primary structure sequence prediction, and circular dichroism spectral data (190–250 nm) showed that NHR3 had a well-defined secondary structure of 25.9% α-helix, 23.2% random coil and 50.9% turn, which was consistent with GOV4 software prediction.

关键词： AML1-ETO NHR3 crystallization ESI mass spectrum circular dichroism spectrum glutathione S-transferase (GST)

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