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Chemoenzymatic Synthesis of Ramosetron Hydrochloride

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Chemical Research in Chinese Universities 2012年第1期28卷 70-72页

作者： LIU Qing-wen1 , XU Hao1, TIAN Hua1, ZHENG Liang-yu2 and ZHANG Suo-qin1 1. College of Chemistry, 2. key laboratory for molecular {3. and engineering, {5. of education, jilin University, changchun 13.012, P. R. China jilin Univ Minist Educ Key Lab Mol Enzymol & Engn Changchun 130012 Peoples R China jilin Univ Minist Educ Coll Chem Changchun 130012 Peoples R China

A new method for the synthesis of ramosetron hydrochloride, the most wildly used 5.HT3.receptor antagonist, was reported. The intermediate 8 was obtained in a high e.e. value via lipase catalyzed hydrolytic kinetic resolution. Then enantiomerically pure ramosetron hydrochloride was synthesized in two steps with excellent yield （the total yield is 24.6%）. The method provides a new environment friendly and atom economy way to synthesize enantiomerically pure ramosetron.

关键词： Ramosetron hydrochloride Chemical enzymatic synthesis Atom economy

Expression,Purification and Crystal Structure of a Truncated Acylpeptide Hydrolase from Aeropyrum pernix K1

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Acta Biochimica et Biophysica Sinica 2005年第9期37卷 613-617页

作者： Hai-Feng ZHANG Bai-Song ZHENG~1 Ying PENG Zhi-Yong LOU Yan FENG~* Zi-He RAO~* laboratory of Structural Biology,Department of Biological Sciences and Biotechnology and Protein Sciences laboratory of ministry of {3.,Tsinghua University,Beijing 100084,China 1 key laboratory for molecular enzymology and engineering of ministry of education,{5. University,changchun 13.023.China laboratoryofStructuralBiology DepartmentofBiologicalSciencesandBiotechnologyandProteinSciencesLaboratoryofM

Acylpeptide hydrolase （APH） catalyzes the N-terminal hydrolysis of Nα-acylpeptides to releaseNα-acylated amino acids. The crystal structure of recombinant APH from the thermophilic archaeon Aeropyrumpernix K1 （apAPH） was reported recently to be at a resolution of 2.1 （?） using X-ray diffraction.A truncatedmutant of apAPH that lacks the first short α-helix at the N-terminal,apAPH-△（1-21）,was cloned,expressed,characterized and *** from biochemical experiments indicate that the optimum temperature ofapAPH is decreased by 15. with the deletion of the N-terminal α-***,the enzyme activity at theoptimal temperature does not *** suggests that this N-terminal α-helix is essential for ***,the crystal structure of apAPH-△（1-21） has been determined by molecular replacement to 2.5.（?）. Acomparison between the two structures suggests a difference in thermostability,and it can be concluded thatby adding or deleting a linking structure （located over different domains）,the stability or even the activity ofan enzyme can be modified.

关键词： acylpeptide hydrolase Aeropyrum pernix K1 crystal structure

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极端酶的研究进展

生物化学与生物物理进展 2002年第6期29卷 847-850页

作者：翁樑冯雁吉林大学分子酶学工程实验室长春130023

极端酶具有超常的生物学稳定性 ,能够在极端温度、pH、压力和离子强度下表现出生物学活性 ,因此极端酶为生物催化和生物转化提供了良机 .新的极端物种的发现、基因组序列的确定及基因工程技术的应用 ,加快了发现和制备新酶的进程 .蛋白... 详细信息

极端酶具有超常的生物学稳定性 ,能够在极端温度、pH、压力和离子强度下表现出生物学活性 ,因此极端酶为生物催化和生物转化提供了良机 .新的极端物种的发现、基因组序列的确定及基因工程技术的应用 ,加快了发现和制备新酶的进程 .蛋白质工程和定向进化技术进一步改善酶的活性和特异性 ,促进了极端酶的工业应用 .对极端酶的研究加深了人们对酶稳定性机制的理解。

关键词：极端酶研究进展异源表达生物催化

Comparison of photovoltaic devices based on MEH-PPV with various molecular weights

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Chinese Science Bulletin 2004年第21期49卷 2259-2261页

作者： KANG Bonan1,2, WANG Liduo1, YANG Yong1 & QIU Yong1 1. key laboratory of Organic Optoelectronics & molecular {3. of ministry of {5., Department of Chemistry, Tsinghua Univer- sity, Beijing 100084, China 2. State key laboratory on Integrated optoelectronics, jilin University Region, changchun 13.023. China key laboratory of Organic Optoelectronics & molecular {3. of ministry of {5. Department of Chemistry Tsinghua University Beijing 100084 China

Polymer photovoltaic devices based on poly (2- methoxy-5.(2′ -ethyl-hexyloxy)-1,4-phenylene vinylene) (MEH- PPV) with three weight-average molecular weights (Mw) have been fabricated with the device structure of ITO/PEDOT/ MEH-PPV/Ca/Ag, and the effect of the molecular weight on photovoltaic properties has been investigated. The experi- mental results show that the high molecular weight of MEH- PPV leads to low series resistance (Rs) and high short-circuit current. The low molecular weight of MEH-PPV leads to high shunt resistance (Rsh) and high open-circuit voltage. When the molecular weight is 6×105. the highest power con- version efficiency was observed.

关键词： MEHPPV 分子量光电器件载流子迁移率串联电阻并联电阻聚(2-甲氧基-5.(2'-乙基-乙氧基)-1,4-亚苯基乙烯撑)

Detection of nucleic acid bases by SERS based on in situ photo-reduced silver colloids

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Detection of nucleic acid bases by SERS based on in situ pho...

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Lei Wu~1,Haoran Xu~1,Zhengqiang Li~1 1.key laboratory for molecular {3. and engineering,the {5. of education,jilin University, changchun,China

In the presence of cyclic organic compounds,and induced by the laser irradiation,the photo-reduced reaction of silver ions could occur and silver colloids could format with the aggregation of silver *** this study,the formation of the colloids induced by laser irradiation was proved by the means of Raman scattering,UV-Vis spectrum and Scanning Electron Microscope(SEM).The Raman signal of nucleic acid bases were slightly

关键词： photo-reduction reaction silver colloidal surface enhanced Raman Scattering detection in situ

Catalytic Versatility of a Novel Mesophilic Bacterial Amylase Secreted by ZW25.0}-1

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Catalytic Versatility of a Novel Mesophilic Bacterial Amylas...

2008年全国生物化学与分子生物学学术大会

作者： Yang Wang Fan Li Ying-jiu Zhang (key laboratory for molecular {3. and engineering,{5. of education,jilin University,changchun 13.021)

INTRODUCTION:A novel bacterial mesophilic amylase,named as oligosaccharide-producing multifunc- tional amylase (OPMA),was discovered and *** was an extraceilular enzyme secreted by ZW25.0}-1 (Fig.1).

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Selection of a Mutant and Optimization of Culture Conditions for the Production of Hyaluronic acid

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Selection of a Mutant and Optimization of Culture Conditions...

国际农业工程大会

作者： Kai-ye DengAssistant Doctor .The key laboratory for Terrain Machine Bionics engineering,{3. of education, {5., jilin 13.025. China jilin AgriculturalUniversity, {5., jilin 13.118, China Lei Yu, Assistant DoctorThe key laboratory for molecular {13. and engineering of {3. ofeducation, {5., jilin 13.025. China jilin Agricultural University, {5., jilin 13.118, China Yu Li, Professorjilin Agricultural University, {5., jilin 13.118, China Lu-quan Ren, ProfessorThe key laboratory for Terrain Machine Bionics engineering, {3. ofeducation, {5., jilin 13.025. China

＜正＞ Hyaluronic acid （Hyaluronan, HA for short）, is a linear polysaccharide composed of repeating disaccharide units of N-acetyl-glucosamine and D-glucuronic acid. The uronic acid and the amino sugar are linked together by alternating beta-1,4 and beta-1,3.glycosidic bonds （Phrem, 1983.. HA exists in many mammalian connective tissues such as joints, vitreous bodies, umbilical cords, cartilages, skins and rooster combs, which contain the polymer at a higher

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Expression of an Intrabody against Human Cyclin Dl Provides Antitumor Efficacy on Hepatocellular Carcinoma both in vitro and in vivo

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Expression of an Intrabody against Human Cyclin Dl Provides ...

“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Bingzhi Li,Dan Yang,Hongliang Xu,Wenhan Wang,Yan Wu,Guiying Li key laboratory for molecular {3. and engineering of the {5. of education,jilin University,changchun,13.021,China

Cyclin D1 is a rate-limiting key factor in controlling the transition from Gl to S in cell *** and overexpression of Cyclin D1 occurs in tumorigenesis of several types of human cancer including hepatocellular carcinoma(HCC).It becomes a potential target for the treatment of ***,by linking appropriate intracellular trafficking signal sequences to their coding genes,can be directed to all intracellular compartments to neutralize and block the function of target *** study was designed to use an intrabody against cyclin D1 to inhibit the growth and proliferation of HCC cells through blocking and inhibiting the biological activity of cyclin D1 overexpressed in cancer *** expression plasmid pER-ADK habouring an endoplasmic reticulum(ER)-retained scFv gene against human Cyclin D1(ER-ADκ) was *** pER-ADK was transfected into HepG2 *** RT-PCR and western blotting showed the anti-cyclinD1 intrabody was expressed efficiently in HepG2 *** assay showed that ER-ADk was localized in the endoplasmic *** results suggested that ER-ADk could recognize and combine its target protein cyclinD1 of HepG2 *** assay showed that ER-ADk significantly inhibited the growth of HepG2 cells(P1).The results of FACS showed that anti-cyclin D1 intrabody arrested most of HepG2 cells at G1 phase and distinctly induced apoptosis of HepG2 cells(P1).ER-ADk expression also suppressed the growth of subcutaneous tumors in nude mice in vivo after the intratumoral injection of *** study suggested that anti-cyclin D1 intrabody inhibits the growth and proliferation of HCC cells both in vitro and in vivo by blocking cyclin D1 activity and it might be a potential approach for hepatocellular carcinoma therapy.

关键词： cyclin D1 intrabody HCC gene therapy

Induction of apoptosis by excess of 2-selenium-bredged-Beta-cyclodexarin through depleting glutathione in cancer cells

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Induction of apoptosis by excess of 2-selenium-bredged-Beta-...

第九届全国酶学学术讨论会暨邹承鲁诞辰85.年纪念会

作者： keyan Zheng Xiaofang Fei Shaowu Lv Ganglin Yan Guimin Luo key laboratory for {3. enzymology and {5. of the ministry of education,jilin University,changchun 13.021,China College of Life Science,jilin University,changchun 13.021,China

Object:Investigation of the biological effect of 2-selenium-bridged β-cyclodextrin (2-SeCD) on cancer cells and its action mechanism.2-SeCD is a glutathione peroxidase mimic synthesized *** low concentration of2-SeCD... 详细信息

关键词： HeLa cells redox status glutathione protein thiols caspase